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隐球壳属(Cryptosphaeria)引起的杨树溃疡病

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山东林业科技 2006年第1期36卷 52-53页

作者：赵桂华吴玉柱陈啸寅谢春芹管斌江苏农林职业技术学院句容212400 山东省林业科学研究院

Cryptosphaeria在欧、美是一类重要病原菌,在我国亦有报道,不仅能引起杨树病害,而且还能引起杨木的腐朽和变色,对杨树的生长潜在着很大的威胁。作者评述了该病的研究历史、分布、危害和发病规律等。

关键词： Cryptosphaeria 杨树溃疡病隐球壳属病原菌

P wave velocity structure of upper and middle crust beneath the Three Gorges reservoir dam and adjacent regions

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Science China Earth Sciences 2009年第4期52卷 567-578页

作者： LI Qiang zhao Xu CAI JinAn LIU RuiFeng LONG guihua AN YanRu National Earthquake Infrastructure Service China Earthquake AdministrationBeijing 100037China China Earthquake Network Center China Earthquake AdministrationBeijing 100045China Institute of Earthquake Science China Earthquake AdministrationBeijing 100036China Institute of Geology and Geophysics Chinese Academy of SciencesBeijing 100029China

The three-dimensional P wave velocity structures of upper and middle crust beneath the Three Gorges reservoir dam and adjacent regions were reconstructed by means of the seismic tomography, using the absolute P-arrivals from the events observed by the Three Gorges seismic network. Our preliminary study of the deep structure shows: (1) the lithology of the Huangling anticline, integral and highly so-lidified, exhibits a uniformity. Down to a depth of 20 km, the Huangling anticline still shows high- velocity anomalies. Note that the characteristics of the velocity images with the different depths are not the same; (2) the depth of the sedimentary rocks beneath the entire Zigui basin is not constant, which is less than 6 km; (3) the top boundary of the crystalline basement varies from 0 to 7 km, whereas the bottom one between 14 and 16 km; (4) the partially molten substances of the upper mantle are located in the Badong-Zigui and its adjacent regions as well as nearby Peishi area. The development and evo-lution of the current tectonics are associated with the cooling and solidifying process of the molten substances within the upper mantle; (5) the different velocity structures of the deep crust in both east and west side of the Jiuwanxi fault are to be investigated in order to understand the origin of gravita-tional gradient zone in the Three Gorge areas; and (6) the velocities within the upper crust would be affected by reservoir water permeability.

关键词： Three Gorges reservoir upper and middle crust three-dimensional velocity structure

Effect of slope position on leaf and fine root C,N and P stoichiometry and rhizosphere soil properties in Tectona grandis plantations

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Journal of Forestry Research 2023年第6期34卷 1997-2009页

作者： Qingqing Zhang Zaizhi Zhou Weiwei zhao guihua Huang Gaofeng Liu Xiaofei Li Junduo Wu Research Institute of Tropical Forestry Chinese Academy of ForestryGuangzhou 510520People’s Republic of China Experimental Center of Tropical Forestry Chinese Academy of ForestryPingxiang 532600People’s Republic of China

Little is known about C-N-P stoichiometries and content in teak(Tectona grandis)plantations in South China,which are mostly sited on hilly areas with lateritic soil,and the effect of slope position on the accumulation of these elements in trees and rhizosphere *** we analyzed the C,N,P content and stoichiometry in leaves,fine roots and rhizosphere soils of trees on the upper and lower slopes of a 12-year-old teak *** Kraft classification system of tree status was used to sample dominant,subdominant and mean trees at each slope *** results showed that the C,N and P contents in leaves were higher than in fine roots and rhizosphere *** lowest C/N,C/P and N/P ratios were found in rhizosphere soils,and the C/N and C/P ratios in fine roots were higher than in *** accumulation in leaves,fine roots and rhizosphere soils were significantly influenced by slope position and tree class with their interaction mainly showing a greater effect on rhizosphere *** C content and C/N ratio,fine root C and P contents,and C/N and C/P ratios all increased distinctly with declining slope *** contents of organic matter(SOM),ammonium(NH4+-N),nitrate-nitrogen(NO3--N)and available potassium(AK)in rhizosphere soils were mainly enriched on upper slopes,but exchange calcium(ECa),available phosphorus(AP),and pH were relatively *** in the C,N and P stoichiometries in trees were mainly attributed to the differences in rhizosphere soil properties.N and P contents showed significant positive linear relationships between leaf and rhizosphere soil,and C content negative linear correlation among leaves,fine roots and rhizosphere *** properties of rhizosphere soils,particularly C/N and NH4+-N,had significant effects on the leaf nutrients in trees on the upper ***,rhizosphere soil properties mainly influenced fine root nutrients on the lower slope,and soil AK was the major influencing ***,these

关键词： Tectona grandis Stoichiometric ratio Rhizosphere soil Dominant trees Nutrient accumulation

全麻患者气管插管套囊压力和术后气道并发症的现况调查及影响因素分析

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临床麻醉学杂志 2018年第8期34卷 733-738页

作者：赵桂华翟晶雯徐江叶张印龙葛明月殷姜文王胜代志刚董希玮徐桂萍新疆石河子大学医学院第一附属医院麻醉科 832000 新疆生产建设兵团第五师医院麻醉科新疆生产建设兵团总医院麻醉科新疆维吾尔自治区人民医院麻醉科

目的调查新疆地区全麻患者气管插管套囊压力及术后插管相关并发症的现状,并分析引起不适套囊压力的影响因素。方法采用整群抽样及单纯随机抽样方法,抽取2017年2—5月新疆地区3家三级甲等医院拟行全身麻醉气管插管并符合纳入标准的患者... 详细信息

目的调查新疆地区全麻患者气管插管套囊压力及术后插管相关并发症的现状,并分析引起不适套囊压力的影响因素。方法采用整群抽样及单纯随机抽样方法,抽取2017年2—5月新疆地区3家三级甲等医院拟行全身麻醉气管插管并符合纳入标准的患者作为研究对象。登记相关信息,于气管插管后采用一次性压力换能器测量套囊压力,拔管后24h内随访患者,记录术后气管插管相关并发症的发生情况。根据测压结果将患者分为套囊压力正常组(20~30cmH_2O)与套囊压力异常组(30cmH_2O),通过Logistic回归分析引起套囊压力过高或过低的危险因素。结果共纳入研究对象430例,气管导管套囊压力为(53.3±20.5)cmH_2O,仅有82例(19.1%)患者套囊压力在推荐范围。术后气道并发症主要有:咽喉痛305例(70.9%),声音嘶哑159例(37.0%),咳嗽147例(34.2%),血丝痰145例(33.7%)。Logistic回归分析显示,BMI≥27kg/m^2(OR=11.000,95%CI 1.064~113.731)、术前气道特殊情况(吸烟、哮喘等,OR=2.809,95%CI1.300~6.070)、麻醉科医师职称(住院医师,OR=60.224,95%CI 18.853~192.380;主治医师,OR=7.364,95%CI 3.112~17.426)和工龄(≤5年,OR=68.500,95%CI 15.253~307.619;6~10年,OR=10.400,95%CI 4.430~24.417)是导致患者出现不适套囊压力升高的危险因素。结论临床工作中套囊压力远高于推荐值,插管相关并发症发生率高,而术前患者肥胖、有吸烟史或合并哮喘、气管炎,麻醉科医师职称较低、工作时间较短是导致气管导管套囊压力异常的危险因素。

关键词：套囊压力气道并发症现况调查危险因素

青海省格尔木市雪山峰地区铜金矿成矿条件浅析

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科技视界 2018年第20期 218-220页

作者：陈巧妹王艳李桂花赵成云青海省第四地质矿产勘查院青海西宁810029 青海省地质矿产测试应用中心青海西宁810001 青海世安矿业勘查开发有限公司青海西宁810000

预查区位于东昆仑山脉西段,大地构造位置地处南昆仑结合带。区内主要出露地层为中-新元古代万保沟群、奥陶纪纳赤台群、三叠纪闹仓坚沟组,构造整体呈北西向和北西西向展布,中酸性侵入岩主要以隐伏体形式存在。雪山峰地区地球化学异常明... 详细信息

预查区位于东昆仑山脉西段,大地构造位置地处南昆仑结合带。区内主要出露地层为中-新元古代万保沟群、奥陶纪纳赤台群、三叠纪闹仓坚沟组,构造整体呈北西向和北西西向展布,中酸性侵入岩主要以隐伏体形式存在。雪山峰地区地球化学异常明显:1:20万化探资料显示雪山峰地区有AS191乙3综合异常,1:5万水系沉积物资料显示该地区有AS1、AS2综合异常。预查区内发现的乌腊德西沟南侧铜矿化点与AS2综合异常套合较好。综合认为,预查区铜金矿成矿地质条件良好。

关键词：雪山峰地区铜金矿成矿条件

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原因不明发热患者尿标本细菌L型的研究

山东医科大学学报 1998年第2期36卷 125-127页

作者：侯桂华赵丽贾继辉李华芬闫世坤丛华山东医科大学微生物学教研室山东医科大学实验中心

研究了４１例原因不明发热患者尿标本中的细菌Ｌ型的分布、超微结构及相关因素。结果表明，细菌Ｌ型的阳性率达６５．８５％，阳性率女性明显高于男性；细菌类型主要为金黄色葡萄球菌和大肠杆菌。由透射电镜可见，Ｌ型菌细胞壁部分缺失... 详细信息

研究了４１例原因不明发热患者尿标本中的细菌Ｌ型的分布、超微结构及相关因素。结果表明，细菌Ｌ型的阳性率达６５．８５％，阳性率女性明显高于男性；细菌类型主要为金黄色葡萄球菌和大肠杆菌。由透射电镜可见，Ｌ型菌细胞壁部分缺失。药敏试验结果表明，头孢唑啉和头孢噻甲羧肟对检出菌及其Ｌ型均有较强地抑制作用；而氨苄青霉素，丁胺卡那霉素、ＳＭＺｃｏ和红霉素对Ｌ型作用较差。

关键词：原因未明热尿细菌L型发热

刚地弓形虫感染对小鼠脑组织转录本m^(6)A甲基化修饰的影响

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中国寄生虫学与寄生虫病杂志 2024年第1期42卷 27-35,47页

作者：解晓曼孙航代莉莎朱文菊王利磊谢环环董宏杰张俊梅王琦周贝贝赵桂华徐超尹昆山东省寄生虫病防治研究所山东第一医科大学(山东省医学科学院)济宁272033

目的分析刚地弓形虫感染对小鼠脑组织转录本的N6-甲基腺苷(m6A)甲基化修饰水平的影响。方法20只雌性C57BL/6J小鼠随机分为TgCtwh6感染组(7只)、LHG感染组(7只)和对照组(TgCtwh6感染组、LHG感染组的对照各3只)。TgCtwh6感染组、LHG感染... 详细信息

目的分析刚地弓形虫感染对小鼠脑组织转录本的N6-甲基腺苷(m6A)甲基化修饰水平的影响。方法20只雌性C57BL/6J小鼠随机分为TgCtwh6感染组(7只)、LHG感染组(7只)和对照组(TgCtwh6感染组、LHG感染组的对照各3只)。TgCtwh6感染组、LHG感染组分别灌胃接种中国Ⅰ型wh6株(TgCtwh6)和中国Ⅲ型LHG株刚地弓形虫感染小鼠脑组织悬液0.2ml(20个包囊/鼠),对照组灌胃等量的生理盐水。分别在接种后15、30和45 d,用抽签法随机抽取感染组小鼠各1只,麻醉后处死,取脑组织,于显微镜下分别记录大脑皮层区、海马区和嗅球区的包囊数。感染后45 d每组分别取3只小鼠的全脑组织,提取总RNA,制备基因组文库,进行转录组测序,筛选差异甲基化位点(DML),统计感染组和对照组的mRNA的差异m6A甲基化位点及其所在转录本;对甲基化位点所在转录本进行基因本体功能注释(GO)分析,京都基因与基因组百科全书(KEGG)富集分析,对甲基化差异转录本进行基因集富集分析(GSEA)。选取甲基转移酶样3(METTL3)、肥胖相关蛋白(FTO)和YTH结构域3(YTHDF3)等3种m6A甲基化修饰蛋白基因,以甘油醛-3-磷酸脱氢酶基因为内参,实时荧光定量逆转录PCR(qRT-PCR)检测其相对转录水平。结果感染组小鼠均感染成功。感染后45 d,大脑皮层、海马和嗅球的包囊数分别为(5676±10)、(4773±9)、(243±10)个。感染组和对照组共检出760650个甲基化位点,其中GGACA、GGACC、GGACT、ACGAT等4种m6A模体的甲基化水平分别占16.8%(127923/760650)、4.6%(35164/760650)、3.8%(28983/760650)和0.4%(3122/760650);共检出高甲基化位点所在转录本127016条,其中GGACA、GGACC、GGACT和ACGAT所在的转录本分别为71727、27754、24556和2979条。感染组与对照组小鼠脑组织转录组中ACGAT、GGACA、GGACC、GGACT等4种m6A模体的DML位点总数为9233,其中高甲基化位点4832个,低甲基化位点4851个。GO分析结果显示,DML所在转录本显著富集于生物过程中的细胞过程、细胞组分中的细胞和分子功能中的结合等功能。DML所在转录本的KEGG富集分析结果显示,分子间相互作用网络主要富集在溶酶体、剪接体和多巴胺能突触信号通路等途径中。对生物过程的GO富集分析结果显示,DML所在的转录本中,生物过程相关的部分转录本主要富集于蛋白糖基化、神经元凋亡负调控、蛋白质的入核转运等过程。GSEA分析结果显示,对照组和两个感染组的组间差异甲基化高分富集于成纤维细胞增殖负调控通路和Hippo信号通路相关的转录本子集中,其中筛选出ENSMUST00000105393、ENSMUST00000006523、ENSMUST00000055261和ENSMUST00000038658等4个关键转录本,分别编码共刺激因子配体(ICOSL)、富含半胱氨酸肠蛋白1(CRIP1)、MOB激酶激活剂1A201(MOB1A201)和MOB1A202,与对照组相比,TgCtwh6感染组、LHG感染组的这4个基因表达均上调。qRT-PCR结果显示,TgCtwh6感染组、LHG感染组小鼠脑组织mettl3 mRNA相对表达水平分别为5.47±1.09、1.63±0.06,与对照组(1.01±0.11)比较差异均有统计学意义(t=4.05、5.03,均P<0.05);ythdf3 mRNA相对表达水平分别为3.57±0.08、1.80±0.25,与对照组(1.01±0.11)比较差异均有统计学意义(t=18.95、2.85,均P<0.05);fto mRNA相对表达水平分别为0.41±0.04、0.60±0.12,与对照组(1.00±0.06)比较差异有统计学意义(t=7.67、2.99,均P<0.05);qRT-PCR结果与转录组测序获得的转录趋势一致。结论弓形虫慢性感染可导致小鼠脑组织转录本m6A甲基化修饰水平升高,并可能通过对icosl、crip1和mob1a等关键差异转录本的修饰调控,造成宿主脑组织内与精神行为相关的生物学进程和信号通路的改变。

关键词：刚地弓形虫 m6A修饰转录本脑包囊纳米孔三代测序

Anisotropic coherence induced nonuniform amplification in N_(2)^(+)

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Science China(Physics,Mechanics & Astronomy) 2024年第12期67卷 17-23页

作者： Hongqiang Xie Qian Zhang Hongbin Lei Na Wang Yuhan Zheng Zhiming Chen guihua Li Zengxiu zhao School of Science East China University of TechnologyNanchang 330013China Department of Physics National University of Defense TechnologyChangsha 410073China School of Science East China Jiaotong UniversityNanchang 330013China

The continuous progress in N_(2)^(+) lasing recently stimulates a great deal of interest in nonlinear and quantum optics of molecular ions,while a complete description of the ionic polarization is still lacking to *** this work,we are dedicated to constructing the fundamental ionic polarization theory where several ubiquitous strong-field processes including ionization,electronic couplings and molecular alignment jointly determine the spatial arrangement of *** the model,the elusive polarization of N_(2)^(+) lasing can be well *** results show that the different electronic transition rules for strong-field ionization and resonant couplings result in peculiar population distributions of various electronic states of N_(2)^(+) in ***,the spatial nonuniformity of population distribution can be aggravated or mitigated during field-free evolutions of coherent molecular rotational wave ***,when a follow-up resonant seed pulse interacts with the prepared ionic system,the anisotropic quantum coherence determining the polarization of subsequent N_(2)^(+) lasing can be *** qualitative agreement between experiments and simulations confirms the validity of the proposed *** findings provide critical insights into the polarization and radiation mechanisms of molecular ions constructed via ultrafast laser pulses.

关键词： anisotropic coherence air lasing nonuniform amplification

可视喉镜联合支气管封堵器与双腔支气管导管在胸科手术麻醉气管插管规范化培训教学中的应用

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中华医学教育探索杂志 2023年第1期22卷 124-127页

作者：段薇娜孔倩孙倩袁敏赵桂华汪华新马莉吴晓静武汉大学人民医院麻醉科武汉430060

目的:探讨可视喉镜联合支气管封堵器与可视喉镜联合双腔支气管导管对麻醉专业住院医师规范化培训学员在胸外科气管插管教学中的应用。方法:将住院医师规范化培训的学员分为对照组和试验组进行临床教学,每组25人。试验组采用可视喉镜联... 详细信息

目的:探讨可视喉镜联合支气管封堵器与可视喉镜联合双腔支气管导管对麻醉专业住院医师规范化培训学员在胸外科气管插管教学中的应用。方法:将住院医师规范化培训的学员分为对照组和试验组进行临床教学,每组25人。试验组采用可视喉镜联合支气管封堵器,对照组采用可视喉镜联合双腔支气管导管。比较两组插管时间、纤支镜定位时间、插管即刻患者血流动力学变化程度、插管成功率、术后并发症发生率及学员考核成绩。采用GraphPad Prism 6.0软件进行t检验和卡方检验。结果:试验组学员气管插管时间[(95.3±10.1)vs.(137.5±13.5)]和定位时间[(100.8±11.7)vs.(155.4±15.3)]均短于对照组(P<0.001),试验组学员插管即刻患者血流动力学变化小(P<0.001)、插管成功率高(92%vs.68%)(P<0.001)、并发症低(P<0.001)、学员考核成绩高(P<0.001)。结论:胸外科手术麻醉教学中气管插管时应用支气管封堵器比双腔支气管导管更能缩短气管插管时间,降低患者插管时血流动力学变化,减轻并发症发生率,以及提高气管插管成功率和增强学员自信心。

关键词：支气管封堵器双腔支气管导管气管插管教学