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Selection of elite lines of BpGH3.5-transgenic Betula platyphylla using growth adaptability analysis

Journal of Forestry Research 2022年第6期33卷 1891-1901页

作者： Chu Wang Yang Wang Jianjun Zou Rusheng Peng Qibin Yu guifeng liu Jing Jiang State Key Laboratory of Tree Genetics and Breeding Northeast Forestry UniversityHarbin 150040People’s Republic of China Jilin Provincial Academy of Forestry Sciences Changchun 130000People’s Republic of China Liaoning Poplar Research Institute Gaizhou 115200People’s Republic of China University of Florida GainesvilleFLUSA

White birch(Betula platyphylla)is precious material for pulpwood and widely distributed in 14 provinces of China.Previous study indicated that inhibited expression of a gene encoding an auxin amide synthase,BpGH3.5,in transgenic plants reduced the level of IAA–amino acid conjugation,resulting in more free IAA,thereby better growth of birch.Utilizing transgenic-B pGH3.5 lines to increase wood production in a wide range of environments is the goal for breeders.In three field trials here,we measured tree height,diameter at breast height,and volume of 16 BpGH3.5-transgenic 7-year-old white birch lines(including 12 antisense strand lines and 4 overexpression lines)and a wild-type white birch line from three sites that varied greatly in their environmental conditions.To select elite BpGH3.5-transgenic lines for each target environment,we used an additive main effects and multiplicative interaction model to analyze genotype by environment interaction,growth adaptability and stability.The selection criteria for elite transgenic lines were set as the average volume plus 0.75 times the standard deviation for the tested lines at each test site.Results showed that the effect of line and site for height was highly significant(P<0.01),and the effect of line×site was significant(P<0.05);selected as the elite lines were FG12,FG13 and FG27 at the Maoershan Experimental Forest Farm,FG13 and FG32 at the Shidaohe Forest Farm,and FG3 and FG31 at the Ecological Experiment Forest Farm.These seven high-yield,stable lines can now be tested in production trials or adjacent trial areas with similar environmental conditions,while the high-yield,unstable lines should be tested in production trials in areas deemed suitable for their growth.These results provide guidance on which released transgenic elite lines will grow best in a wide range of conditions.

关键词： Betula platyphylla BpGH3.5 Afforestation test Growth adaptability

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政府开放数据平台FAIR原则评估指标体系及实证研究

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现代情报 2024年第2期44卷 4-16页

作者：刘桂锋陈书贤刘琼江苏大学科技信息研究所江苏镇江212013 江苏大学图书馆江苏镇江212013

[目的/意义]评估政府开放数据平台FAIR原则的实施效果有助于平台规范标识符、统一元数据标准等,增强政府数据的发现、共享和重用。[方法/过程]采用网络调研法,借鉴国际代表性评估框架,结合调研构建面向政府开放数据平台的FAIR原则评估... 详细信息

[目的/意义]评估政府开放数据平台FAIR原则的实施效果有助于平台规范标识符、统一元数据标准等,增强政府数据的发现、共享和重用。[方法/过程]采用网络调研法,借鉴国际代表性评估框架,结合调研构建面向政府开放数据平台的FAIR原则评估指标体系,并选取国内外8个样本平台作为案例,从可发现、可访问等4个维度展开评估分析,比较FAIR原则实施状况和差异。[结果/结论]从永久性标识符方案、元数据标准规范、数据使用许可等方面提出相应改进策略,完善国内政府开放数据平台数据管理规范,为后续相关平台的建设与利用提供理论指导和实践借鉴。

关键词：政府开放数据数据平台 FAIR原则数据科学开放科学

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Olfactory ensheathing cell transplantation improves sympathetic skin responses in chronic spinal cord injury

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Neural Regeneration Research 2013年第30期8卷 2849-2855页

作者： Zuncheng Zheng guifeng liu Yuexia Chen Shugang Wei Department of Orthopedics Taian Central Hospital Department of Spine and Spinal Cord Taian Rongjun Hospital

Forty-three patients with chronic spinal cord injury for over 6 months were transplanted with bryonic olfactory ensheathing cells, 2-4 × 106, into multiple sites in the injured area under the sur-gical microscope. The sympathetic skin response in patients was measured with an electromyo-graphy/evoked potential instrument 1 day before transplantation and 3-8 weeks after trans-tion. Spinal nerve function of patients was assessed using the American Spinal Injury Association impairment scale. The sympathetic skin response was elicited in 32 cases before olfactory en-sheathing celltransplantation, while it was observed in 34 cases after transplantation. tantly, sympathetic skin response latency decreased significantly and amplitude increased cantly after transplantation. Transplantation of olfactory ensheathing cells also improved American Spinal Injury Association scores for movement, pain and light touch. Our findings indicate that factory ensheathing celltransplantation improves motor, sensory and autonomic nerve functions in patients with chronic spinal cord injury.

关键词： neural regeneration spinal cord injury clinical practice olfactory ensheathing cells cell transplan-tation olfactory ensheathing cell transplantation sympathetic skin response neurological function autonomic nerve paralysis neuroregeneration

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AIGC赋能图书馆阅读推广智慧服务的框架和应用研究

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图书馆学研究 2024年第2期 108-118,107页

作者：刘琼刘桂锋王鹏江苏大学图书馆江苏大学

AIGC技术赋能图书馆阅读推广服务,可以为图书馆阅读推广从数字化向智能化转型注入核心驱动力,推动阅读推广的高质量发展。从阅读推广实践工作和智慧图书馆发展两方面梳理图书馆阅读推广智慧服务的必要性,构建图书馆阅读推广智慧服务的框... 详细信息

AIGC技术赋能图书馆阅读推广服务,可以为图书馆阅读推广从数字化向智能化转型注入核心驱动力,推动阅读推广的高质量发展。从阅读推广实践工作和智慧图书馆发展两方面梳理图书馆阅读推广智慧服务的必要性,构建图书馆阅读推广智慧服务的框架,采用GitHub托管平台和ChatGPT在腾讯QQ聊天软件中部署虚拟数字人,作为“馆员助手”辅助活动实践和阅读文化创作,加入QQ群,作为“虚拟馆员”为读者提供导读和咨询。AIGC工具作为“馆员助手”,能出色地协助完成阅读推广的文本处理和创作工作,但作为“虚拟馆员”与读者进行“一对多”交互的效果较差。图书馆应主动树立AIGC技术的理念意识,建立阅读推广信息库,提升馆员的人工智能素养,推进AIGC与阅读推广服务融合,深化阅读推广的智慧化服务。

关键词： AIGC 阅读推广智慧服务 ChatGPT 虚拟馆员

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期刊论文支撑数据FAIR原则的应用评估与案例分析

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现代情报 2024年第2期44卷 17-29页

作者：刘桂锋王清炫韩牧哲江苏大学科技信息研究所江苏镇江212013 江苏大学图书馆江苏镇江212013

[目的/意义]FAIR原则在科技期刊的应用有助于增强期刊论文支撑数据的可发现、交互、共享与重用。评估FAIR实施情况有利于其推广实施,以期为我国期刊论文的支撑数据共享与重用提供有益参考。[方法/过程]本文在国外FAIR原则评估模型的基础... 详细信息

[目的/意义]FAIR原则在科技期刊的应用有助于增强期刊论文支撑数据的可发现、交互、共享与重用。评估FAIR实施情况有利于其推广实施,以期为我国期刊论文的支撑数据共享与重用提供有益参考。[方法/过程]本文在国外FAIR原则评估模型的基础上,综合考虑各模型的优势和指标设计特点,结合《数据分析与知识发现》期刊论文相关的科学数据特征,构建FAIR原则指标评价体系,基于该体系从4个维度分析调研结果,最后对中文期刊论文的支撑数据FAIR应用提出合理化建议与优化策略。[结果/结论]FAIR原则在期刊论文支撑数据的应用仍需进一步完善,科研人员的数据共享意识及对于FAIR原则的认知度远远不够,建议从宏观和微观两个层面推广FAIR原则及其实施,推动数据更加开放和可重用。

关键词： FAIR原则期刊论文支撑数据数据管理数据科学应用评估案例分析

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Progeny test of tetraploid Betula platyphylla and preliminary selection of hybrid parents

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Journal of Forestry Research 2016年第3期27卷 665-674页

作者： Huanwen Xu Yu liu Jing Jiang guifeng liu Xiyang Zhao State Key Laboratory of Tree Genetics and Breeding Northeast Forestry University

Estimating genetic parameters of parental lines through progeny testing and choosing good hybrid parents are important for genetically improving seed orchard trees.In this study, 24 tetraploid progeny seedlings were used as experimental materials, which came from test cross design：six tetraploid Betula platyphylla lines（Q33, Q13, Q103,Q19, Q83 and Q14） as female parents and four individual B. platyphylla diploid lines（F3, F4, F9 and F11） as male parents were crossed. Variance analysis of height, diameter, height-to-diameter ratio, and internodal distance showed that the differences between hybrid combinations reached highly significant levels. Using multi-objective decisionmaking, we performed a comprehensive assessment of the various hybrid combinations. Using a selection rate of 20 % of the standard, five hybrids were selected;their genetic gains in average height, diameter, height-todiameter ratio, and internodal distance were 20.95, 6.07,13.07 and 8.96 %, respectively. We also analyzed the combining ability and genetic parameter effect values of parents and hybrid combinations. The combined analysis revealed that Q13, Q103, Q33 and Q83 were superior females; F3, F4, and F9 were superior males; and F3 × Q13, F4 × Q83 and F9 × Q33 were superior hybrid combinations. The heights and diameters of these progenies were 22.49 and 11.48 % greater than average,respectively.

关键词： Betula platyphylla Combining ability Testcross

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Expression profiling of the BpIAA gene family and the determination of IAA levels in Betula platyphylla tetraploids

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Journal of Forestry Research 2019年第3期30卷 855-867页

作者： Wendi Xu Su Chen Jing Jiang guifeng liu State Key Laboratory of Forest Genetics and Tree Breeding Northeast Forestry University

Betula platyphylla Sukaczev tetraploids have significantly larger leaf, fruit and stoma (gigantic phenotype) than diploids of the same species;however, the mechanism underlying this difference remains unclear. Tetraploid B. platyphylla transcriptome data have indicated that the expression of genes related to indole-3-acetic acid (IAA) biosynthesis and signal transduction was altered after genome duplication. IAA exerts pleiotropic effects on growth and development by inducing the expression of Aux/IAA. We identified 20 Aux/IAA genes (BpIAA1– BpIAA20) in B. platyphylla distributed across 10 chromosomes. Multiple alignment and motif analyses revealed that nine BpIAA proteins shared all four conserved domains. Phylogenetic analysis indicated that Aux/IAA families were divided into four subfamilies and that there were two pairs of BpIAA sister genes. The BpIAAs were differentially expressed in diploids and tetraploids. Moreover, the expression levels of the nine BpIAA genes were specifically up-regulated in tetraploids from June to September compared with May (except August 5th) in tetraploids, while they were down-regulated in diploids. IAA levels were more than twofold higher in tetraploids than diploids during the vegetative season. These results indicate that genome duplication of B. platyphylla caused the up-regulated of genes involved in IAA synthesis, and the increased concentration of IAA may induce the constitutive expression of 20 BpIAA genes. Therefore, the significant changes in the expression patterns of the BpIAAs contributed to the gigantic phenotype of tetraploids to some extent. Our research sheds light on the phenotypic variations observed in B. platyphylla tetraploids.

关键词： Betula platyphylla Suk. Tetraploid Aux/IAA Expression profile IAA

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基于FAIR原则的数据期刊开放共享与关联模型研究

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现代情报 2024年第2期44卷 30-42,91页

作者：刘桂锋陈帅印韩牧哲江苏大学科技信息研究所江苏镇江212013 江苏大学图书馆江苏镇江212013

[目的/意义]为揭示数据论文与期刊论文关联出版的新形态,对目前数据期刊的开放共享、数据论文与期刊论文之间的关联进行研究,有助于推动科学数据的开放共享发展,促进科学数据的高效流通,使科学数据在多层维度释放数据价值。[方法/过程]... 详细信息

[目的/意义]为揭示数据论文与期刊论文关联出版的新形态,对目前数据期刊的开放共享、数据论文与期刊论文之间的关联进行研究,有助于推动科学数据的开放共享发展,促进科学数据的高效流通,使科学数据在多层维度释放数据价值。[方法/过程]基于FAIR原则,从元数据元素、文献服务等角度出发,构建数据流向视角下数据论文与期刊论文之间的互关联模型,分析数据论文与期刊论文之间的关联过程,并选取代表性数据期刊Data in Brief的数据论文为实例展开模型验证与实践参照。[结果/结论]本文基于“可访问”“可发现”对“开放共享”展开研究;基于“可互操作”和“可重用”对“关联”展开研究。通过构建理论模型、实例验证,厘清数据论文与期刊论文之间的关联模式以及验证理论模型的可行性与合理性。

关键词： FAIR原则数据论文期刊论文数据期刊科学数据数据科学

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社会科学学者中文论文代表作抽选的影响因素

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图书馆论坛 2024年第1期44卷 86-93页

作者：包翔王世鹏刘桂锋江苏大学图书馆江苏大学科技信息研究所

代表作是评价学者学术水平的重要依据,探究学者在代表作抽选时的影响因素,对学者学术水平的展示和代表作制度的实施具有重要意义。文章结合代表作特点,以管理学、法学和图书馆、情报与文献学等3个社会科学学科的学者作为研究对象,归纳... 详细信息

代表作是评价学者学术水平的重要依据,探究学者在代表作抽选时的影响因素,对学者学术水平的展示和代表作制度的实施具有重要意义。文章结合代表作特点,以管理学、法学和图书馆、情报与文献学等3个社会科学学科的学者作为研究对象,归纳学者在代表作抽选过程中的影响因素并设计李克特五级量表,通过在因子分析中提取特征值大于1的主要因素,采用最大方差旋转法归纳修正后的因素,最终构建代表作抽选的多元回归模型。研究发现:代表作的学术属性、新颖属性、社会属性、时间属性、传承属性等5种因素是影响社科学者代表作抽选的主要因素,不同学科学者在代表作抽选时的侧重点不同。

关键词：代表作抽选因子分析回归模型

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Analysis of three types of triterpenoids in tetraploid white birches(Betula platyphylla Suk.) and selection of plus trees

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Journal of Forestry Research 2015年第3期26卷 623-633页

作者： Sui Wang Hui Zhao Jing Jiang guifeng liu Chuanping Yang State Key Laboratory of Tree Genetics and Breeding Northeast Forestry University

Betulin, oleanolic acid, and betulinic acid are naturally occurring pentacyclic triterpenoids that have significant medicinal value. Considerable amounts of these triterpenoids are available in the outer bark of white birch. In this study, we used ultrasound-assisted extraction （UAE） to extract triterpenoids from birch bark rapidly and with high efficiency. Using high performance liquid chro- matography （HPLC）, three types of triterpenoids were separated and detected. We examined the differences among triterpenoids extracted from diploid versus tetra- ploid white birch. Then, we used factor analysis to screen out tetraploid white birches with comprehensively excel- lent performance. The results indicate that the optimum conditions for extraction include the use of ethanol as an extraction solvent, a solid-to-liquid ratio of 0.1 g/10 ml, ultrasonic power set at 100 W, a temperature of 60 ℃ and an extraction time of 15 min. A reversed-phase C18 col- umn （4.6 mm × 250 mm × 5 μm） with a column tem- perature of 30 ℃ and the mobile phase composed of A （acetonitrile） and B （0.1% aqueous phosphoric acid, v/v） at a flow rate of 0.5 ml/min were used, and the detection wavelength was 195 nm. No significant difference wasobserved between diploid and tetraploid white birch in terms of the content of three types of triterpenoids （at a confidence level of 0.05）. As triterpenoid content, height, and DBH （diameter at breast height） are strongly interre- lated, we used factor analysis to evaluate all individuals, and we screened out six plus trees with excellent com- prehensive characters.

关键词： Betula platyphylla Suk Triterpenoids Highperformance liquid chromatography （HPLC） Tetraploid Diploid Factor analysis

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