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HPLC法测定辣椒红色素中苯甲酸的含量

食品与发酵工业 2006年第8期32卷 109-111页

作者：徐公世隋晓杜桂彩青岛大学天然色素研究所

建立了辣椒红色素中苯甲酸含量的高效液相色谱分析法.样品经亚铁氰化钾和乙酸锌溶液沉淀后,再依次经滤纸和0.45 μm的滤膜过滤,滤液进行高压液相色谱分析,色谱柱为ODSC18柱,流动相为乙腈-乙酸铵溶液,流速为0.7 mL/min,检测波长为230 nm... 详细信息

建立了辣椒红色素中苯甲酸含量的高效液相色谱分析法.样品经亚铁氰化钾和乙酸锌溶液沉淀后,再依次经滤纸和0.45 μm的滤膜过滤,滤液进行高压液相色谱分析,色谱柱为ODSC18柱,流动相为乙腈-乙酸铵溶液,流速为0.7 mL/min,检测波长为230 nm,该方法的回收率为98.2%～101.7%,相对偏差在1.56%～2.00%之间.此法不仅精密度高、准确而且操作非常方便.

关键词：高效液相色谱法辣椒红色素苯甲酸

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利用辣椒渣提取不溶性膳食纤维的研究

食品与发酵工业 2006年第4期32卷 130-132页

作者：汪靖超杨宏姚海军杜桂彩青岛大学生物系青岛266071 青岛大学天然色素研究所青岛266071

辣椒渣是红辣椒提取色素和辣椒碱后的产物,用酸碱化学处理法处理辣椒渣,得到不溶性膳食纤维,产率为14%,纤维素含量达到86.79%,产品不含苯甲酸和辣椒碱,Pb和As的含量分别<20mg/kg和2mg/kg。

关键词：辣椒渣水不溶性膳食纤维酸碱法

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玉米黄素糖基化酶在大肠杆菌中的表达与纯化

食品与生物技术学报 2015年第8期34卷 886-890页

作者：汪靖超滕守振陈秀鹏杜桂彩郭群群李荣贵青岛大学生命科学学院山东青岛266071

噬夏孢欧文氏菌合成类胡萝卜素涉及的一系列反应都是在相关酶的催化下完成的,其中催化玉米黄素转化为玉米黄素二葡萄糖苷的玉米黄素糖基化酶由基因crt X编码。PCR扩增出噬夏孢欧文氏菌crt X基因,构建重组质粒,转化大肠杆菌。经镍离子树... 详细信息

噬夏孢欧文氏菌合成类胡萝卜素涉及的一系列反应都是在相关酶的催化下完成的,其中催化玉米黄素转化为玉米黄素二葡萄糖苷的玉米黄素糖基化酶由基因crt X编码。PCR扩增出噬夏孢欧文氏菌crt X基因,构建重组质粒,转化大肠杆菌。经镍离子树脂亲和层析、DEAESepharose FF离子交换层析纯化,得到了电泳纯的重组噬夏孢欧文氏菌玉米黄素糖基化酶,该重组蛋白在体外具有催化玉米黄素转化为玉米黄素二葡萄糖苷的活性。

关键词：噬夏孢欧文氏菌玉米黄素糖基化酶活性

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鼠尾藻多酚的抗凝血活性研究

中药新药与临床药理 2007年第3期18卷 191-194页

作者：李敬魏玉西杜桂彩胡迎芬李丽中国科学院海洋研究所山东青岛266071 青岛大学生物系山东青岛266071

目的探讨鼠尾藻多酚STP-1和STP-2的抗凝血活性。方法体内实验分别以毛细管法、剪尾法测定鼠尾藻多酚对小鼠凝血时间、出血时间的影响;体外实验分别测定鼠尾藻多酚对新西兰兔血浆活化部分凝血活酶时间、血浆凝血酶原时间、血浆凝血酶时... 详细信息

目的探讨鼠尾藻多酚STP-1和STP-2的抗凝血活性。方法体内实验分别以毛细管法、剪尾法测定鼠尾藻多酚对小鼠凝血时间、出血时间的影响;体外实验分别测定鼠尾藻多酚对新西兰兔血浆活化部分凝血活酶时间、血浆凝血酶原时间、血浆凝血酶时间的影响。结果鼠尾藻多酚STP-1在体内、体外均有显著的抗凝血作用,而且中剂量组(1.0mg/mL)抗凝血活性最佳。结论鼠尾藻多酚STP-1在体内、外均有抗凝血活性。

关键词：鼠尾藻多酚抗凝血活性

杀松材线虫海洋真菌ML-5的鉴定及培养条件研究

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青岛大学学报（工程技术版） 2015年第2期30卷 102-107页

作者：李子周长景陈聪聪杜桂彩李丽郭道森青岛大学生命科学学院山东青岛266071

针对危害松属植物的重要病原物松材线虫，本文对采自青岛附近海域的样品进行真菌分离，采用浸渍法对分离得到的35株真菌的培养滤液进行杀线活性测定，从中筛选得到了1株具有高杀松材线虫活性的真菌ML-5，用其培养滤液处理松材线虫72h时... 详细信息

针对危害松属植物的重要病原物松材线虫，本文对采自青岛附近海域的样品进行真菌分离，采用浸渍法对分离得到的35株真菌的培养滤液进行杀线活性测定，从中筛选得到了1株具有高杀松材线虫活性的真菌ML-5，用其培养滤液处理松材线虫72h时，线虫的校正死亡率可达86．51％。通过形态学观察和18SrDNA序列分析，将其鉴定为青霉属（Penicillium）的一种真菌。对ML-5的培养条件进行了研究，确定菌株ML-5在PD培养基中杀线活性最佳的培养条件是：100％海水浓度，初始pH值5．0～6．0，培养温度25℃，培养时间7d。该研究为海洋真菌活性物质的开发及在松萎蔫病防治方面的应用提供了理论依据。

关键词：海洋真菌鉴定青霉菌松材线虫杀线活性培养条件

Empirical Analysis of City Contact in Zhujiang(Pearl) River Delta, China

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Chinese Geographical Science 2014年第3期24卷 384-392页

作者： TONG De LIU Tao LI guicai YU Lei Peking University Shenzhen Graduate School Zhejiang Province Development Planning & Research Institute

In traditional urban geography, city contact research is a classic study element in city research. In general, researchers use the traditional gravity model to characterize the contacts that exist between two cities. The traditional gravity model assumes ideal conditions, but these preconditions and their results often do not exist in realistic conditions. Thus, we used a modified gravity model to characterize the city contacts within a specific region. This model considers factors such as intercity complementarities, government intervention, and the diversity of the transportation infrastructure which is characterized as the transportation distance instead of the traditional Euclidean distance. We applied this model to an empirical study of city contact in the Zhujiang(Pearl) River Delta(PRD) of China. The regression results indicated that the modified gravity model could measure city contact more accurately and comprehensively than the traditional gravity model, i.e., it yielded a higher adjusted R2 value(0.379) than the traditional gravity model result(0.259). Our study also suggests that, in addition to urban-regional and metropolitan development, the complementarities of the basic functions of cities at the administrative and market levels, as well as the corporeal and immaterial levels, play very significant roles in the characterization of city contact. Given the complexity of city contact, it will be necessary to consider more relevant influential factors in the modified gravity model to characterize the features of city contact in the future.

关键词： city contact function complementarities government intervention gravity model Zhujiang (Pearl) River Delta (PRD) China

Copper Indium Sulfide Enables Li-CO_(2)Batteries with Boosted Reaction Kinetics and Cycling Stability

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Energy & Environmental Materials 2023年第5期6卷 372-380页

作者： Lin Chen Jingwen Zhou Junxiang Zhang guicai Qi Bin Wang Jianli Cheng Institute of Chemical Materials China Academy of Engineering PhysicsMianyang 621900China Yangtze Delta Region Institute Institute of Fundamental and Frontiers SciencesUniversity of Electronic Sciences and Technology of ChinaHuzhou 313001China Institute of Fundamental and Frontiers Sciences University of Electronic Sciences and Technology of ChinaChengdu 611731China Department of Chemistry City University of Hong KongKowloon Hong Kong China

High energy density Li-CO_(2)batteries have attracted much attention owing to the"two birds with one stone"feature in fixing greenhouse gas CO_(2)and providing renewable ***,poor reversibility of the discharge product Li_(2)CO_(3)is one of the main problems that limit its application,resulting in poor cycling stability and severe ***,copper indium sulfide(CIS),a semiconducting non-precious metal sulfide,is fabricated as cathode catalysts for high-performance Li-CO_(2)*** with the synergistic effect of bimetallic valence bonding and coordinated electron transfer,Li-CO_(2)batteries using CIS cathodes exhibit high full specific discharge capacity,excellent rate capability and cycle stability,namely it delivers a high specific full discharge capacity of 8878μAh cm^(-2),runs steadily from 10 to 100μA cm^(-2),and performs a stable long-term cycling behavior(>1050 h)under a high energy efficiency of 84%and a low charge voltage of approximately 3.4 V at 20μA cm^(-2)within 100μAh cm^(-2).In addition,a flexible Li-CO_(2)pouch cell is constructed to reveal the potential of employing CIS to fabricate flexible high energy storage devices in practical *** work shows a promising development pathway toward next-generation sustainable energy storage devices.

关键词： copper indium sulfides cycling stability Li-CO_(2)batteries reaction kinetics

Analysis of the Properties and Anti-Seepage Mechanism of PBFC Slurry in Landfill

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Structural durability & Health Monitoring 2017年第2期11卷 169-190页

作者： Guozhong Dai Jia Zhu guicai Shi Yanmin Sheng Shujin Li School of Civil Engineering&Architecture Changzhou Institute of Technology666 Liaohe RoadChangzhou213032P.R.China

As the landfill leachate has strong pollution on the underground water,surface water and *** paper develops the formula of impervious slurry with low permeability,good durability,strong adsorption and retardant based on the bentonite which is modified by polyvinyl *** the simulation experiment,the optimum formula of polyvinyl alcohol is 0.2%.Its osmotic coefficient for 28 days is 0.53×10^-8~1.86×10^-8 cm/s and compressive strength is 0.5~1.5 MPa as *** paper study on the retardant rule of the consolidation of slurry against the pollution in the leachate by self-made percolation *** experiment shows that the retardant rate of the consolidation against inorganic pollutants and organic pollutants is over 85% and the retardant rate against heavy metal ion such as Hg and Pb is above 99%.The slurry has the characteristics of low permeability,high retardant against pollution,good durability and plasticity,no chemical additives,no pollution,wide source of raw materials and good economy which determine it can be used to new landfill or existing landfill,building foundation pit and water conservancy project.

关键词： Waste landfill anti-seepage slurry seepage resistance adsorption property corrosion resistance durability mechanical property

FreestandingMo_(3)N_(2) nanotubes for long-term stabilized 2e−intermediate-based high energy efficiency Li-CO_(2) batteries

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SusMat 2023年第2期3卷 276-288页

作者： guicai Qi Junxiang Zhang Jianli Cheng Bin Wang Institute of Chemical Materials China Academy of Engineering PhysicsChengduChina Institute of Fundamental and Frontier Sciences University of Electronic Science and Technology of ChinaChengduChina

Li–CO_(2) batteries are considered one of the promising power sources owing to ultrahigh energy density and carbon ***,the sluggish reaction kinetics of 4e−discharged process(Li_(2)CO_(3))impede its potential *** of the efficient strategies for developing cathode catalysts is to stabilize 2e−intermediate Li_(2)C_(2)O_(4) and improve reaction ***,longterm catalysts of stabilized Li_(2)C_(2)O_(4) are barely achieved,whereas cycle stability is far from satisfactory ***,non-noble metal–based Mo_(3)N_(2) is synthesized and employed as freestanding cathodes for Li–CO_(2) *** to rich delocalized electrons of Mo^(2+)and reversible electron localization structure,freestanding Mo_(3)N_(2) cathodes exhibit a low charge potential(3.28 V)with an ultralow potential gap(0.64 V),high energy efficiency of up to 80.46%,fast rate capability,and outstanding cycle stability(>910 h).In situ experiments and theoretical calculation verify that Mo_(3)N_(2) stabilizes 2e−Li_(2)C_(2)O_(4) intermediate by the interaction of Mo^(2+)as active sites where Mo^(2+)promotes the transfer of outer electrons to O,prevents its disproportionation to Li_(2)CO_(3),and promotes reaction kinetics,contributing to high energy efficiency and outstanding cycle *** addition,the pouch-cells deliver ultrahigh energy density of up to 6350.7W h kg^(−1) based on the mass of cathode materials.

关键词： carbon-free freestanding high energy efficiency Li-CO_(2)batteries Mo_(3)N_(2)nanotubes