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Structural measurements and vessel density of spectraldomain optic coherence tomography in early,moderate,and severe primary angle-closure glaucoma

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International Journal of Ophthalmology(English edition) 2023年第7期16卷 1100-1109页

作者： Wei Jiang Nan Jiang gui-Bo Liu Jing Lin Cui Li gui-qiu zhao Department of Ophthalmology the Affiliated Hospital of Qingdao UniversityQingdao 266000Shandong ProvinceChina

AIM:To compare the macular ganglion cell-inner plexiform layer(GCIPL)thickness,retinal nerve fiber layer(RNFL)thickness,optic nerve head(ONH)parameters,and retinal vessel density(VD)measured by spectral-domain optical coherence tomography(SD-OCT)and analyze the correlations between them in the early,moderate,severe primary angle-closure glaucoma(PACG)and normal ***:Totally 70 PACG eyes and 20 normal eyes were recruited for this retrospective *** eyes were further separated into early,moderate,or severe PACG eyes using the Enhanced Glaucoma Staging System(GSS2).The GCIPL thickness,RNFL thickness,ONH parameters,and retinal VD were measured by SD-OCT,differences among the groups and correlations within the same group were ***:The inferior and superotemporal sectors of the GCIPL thickness,rim area of ONH,average and inferior sector of the retinal VD were significantly reduced(all P<0.05)in the early PACG eyes compared to the normal and the optic disc area,cup to disc ratio(C/D),and cup volume were significantly higher(all P<0.05);but the RNFL was not significant changes in early and moderate *** severe group,the GCIPL and RNFL thickness were obvious thinning with retinal VD were decreasing as well as C/D and cup volume increasing than other three groups(all P<0.01).In the early PACG subgroup,there were significant positive correlations between retinal VD and GCIPL thickness(except superonasal and inferonasal sectors,r=0.573 to 0.641,all P<0.05),superior sectors of RNFL thickness(r=0.055,P=0.049).More obvious significant positive correlations were existed in moderate PACG eyes between retinal VD and superior sectors of RNFL thickness(r=0.650,P=0.022),and temporal sectors of RNFL thickness(r=0.740,P=0.006).In the severe PACG eyes,neither GCIPL nor RNFL thickness was associated with retinal ***:The ONH damage and retinal VD loss appears earlier than RNFL thickness loss in PACG *** the PACG disease progressed from the earl

关键词： optic coherence tomography primary angle-closure glaucoma ganglion cell-inner plexiform layer retinal nerve fiber layer optic nerve head retinal vessel density

TREM-1 expression in rat corneal epithelium with Aspergillus fumigatus infection

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International Journal of Ophthalmology(English edition) 2015年第2期8卷 222-227页

作者： Li-Ting Hu zhao-Dong Du gui-qiu zhao Sheng qiu Nan Jiang Jing Lin Qian Wang Qiang Xu Department of Ophthalmology Affiliated Hospital of Qingdao University

AIM: To investigate the expression of triggering receptor expressed on myeloid cells-1(TREM-1) in the aberrant inflammation within the corneal epithelium at early period of fungal ***: A total of 65 Wistar rats were randomly divided into control group, sham group and fungal keratitis(FK) group, in which the cornea was infected by Aspergillus fumigatus(A. fumigatus). After executed randomly at 8, 16, 24, 48 and 72 h after experimental model being established, the severity of keratomycosis in rats was scored visually with the aid of a dissecting microscope and slit lamp. Then corneas in three groups were collected to assess the expression of TREM-1through quantitative reverse transcription-polymerase chain reaction(RT-PCR), immunofluorescence technique and Western blot analysis. The correlation between FK inflammation and expression of TREM-1 was also ***: Corneal inflammation scores increased with time after fungal infection(F =49.74, P =0.000). The inflammation scores in FK group were obviously higher than those in sham group on the whole(F =137.78, P =0.000). Levels of TREM-1 in the infected rat corneal epithelium had elevated at 8h and peaked at 48h(P <0.001,compared with control group). Western blot analysis also showed an obviously elevated TREM-1 level in rat corneal epithelium at 24 h and 48 h after fungal *** technique showed that TREM-1mainly existed in corneal epithelium and infected corneal stoma of rat. TREM-1 protein expression was enhanced after fungal infection. Moreover, severity of FK inflammation was significantly related to TREM-1expression in FK(r =0.942, P =0.000).CONCLUSION: TREM-1 may contribute to amplify theinflammation in the cornea infected with A. fumigatus and play critical roles in the battle against A. fumigatus in the innate immune responses.

关键词： fungal keratitis triggering receptor expressed on myeloid cells-1 Aspergillus fumigatus corneal epithelium

Natamycin in the treatment of fungal keratitis : a systematic review and Meta-analysis

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International Journal of Ophthalmology(English edition) 2015年第3期8卷 597-602页

作者： Sheng qiu gui-qiu zhao Jing Lin Xue Wang Li-Ting Hu zhao-Dong Du Qian Wang Cheng-Cheng Zhu Department of Ophthalmology Medical College Qingdao University Department of Ophthalmology the Affiliated Hospital of Qingdao University

AIM: To review published clinical studies examining the effect of natamycin in the treatment of fungal ***: We selected the publications in CENTRAL,MEDLINE, EMBASE, CNKI, and CBM. This study systematically reviewed published randomized controlled trials(RCTs) that compared natamycin to other antifungal agents, and conducted feasible Meta-analysis of efficacy results using Revman 5.2 ***: We included seven trials which were mainly carried out in developing countries of Asia, with five trials conducted in India, one each in China and Bangladesh. A total of 804 participants were randomized to following comparisons: 2% econazole versus 5%natamycin showed little difference in the effects of treatment of fungal keratitis [RR =0.99, 95% confidence interval(CI), 0.8 to 1.21]; chlorhexidine gluconate versus5% natamycin indicated that the results on healing of the ulcer at 21 d was less conclusive(RR=0.77, 95% CI, 0.55 to 1.08; I2=0%); 1% voriconazole versus 5% natamycin suggested that natamycin treatment appeared to be significantly better outcomes than voriconazole(regression coefficient =-0.18 log MAR; 95% CI,-0.30 to-0.05; P =0.006), especially in Fusarium cases(regression coefficient=-0.41 log MAR; 95% CI,-0.61 to-0.20; P <0.001);natamycin versus fluconazole showed a significant difference in cure rate(χ2=5.048, P <0.05) and natamycin group was more effective than fluconazole in average period of therapy(t =7.94, P <0.01).CONCLUSION: Natamycin was a preferable choice in the treatment of fungal keratitis, especially in the early period of Fusarium cases.

关键词： eye infection fungal natamycin Metaanalysis

Co-regulation of Dectin-1 and TLR2 in inflammatory response of human corneal epithelial cells induced by Aspergillus fumigates

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International Journal of Ophthalmology(English edition) 2016年第2期9卷 185-190页

作者： gui-qiu zhao Xue-Yan qiu Jing Lin Qing Li Li-Ting Hu Qian Wang Hui Li Department of Ophthalmology the Affiliated Hospital of Qingdao University

AIM： To investigate the co-regulation of dendritic cell- associated C-type lectin-1 （Dectin-1）, Toll-like receptor 2 （TLR2）, and relative chemotactic factors in the Telomease- immortalized human corneal epithelial （THCE） cells after exposure to ,Aspergillus fumigatus （Af） hyphae. METHODS： The normal THCE cells were investigated as control. After cultured in vitro with Af hyphae, with or without laminarin and anti-TLR2 antibody for 4, 8, 16 and 24h, THCE cells were harvested. The expression of Dectin-1, TLR2, CXCL1 and CXCL8 mRNA were measured by real-time quantitative polymerase chain reaction at the stimulation of 4, 8 and 16h separately. The protein expression of Dectin-1 and TLR2 were analyzed at 8, 16, and 24h by Western blot. ~ RESULTS： The mRNA CXCL8 increased in THCE expression of CXCL1 and cells after stimulated by Af hyphae. The stimulatory effects on these inflammatory chemokines were shown in a dose-dependent manner and reached the peak at 8h. Af hyphae significantly stimulated the production of Dectin-1 and TLR2 in THCE cells at both mRNA and protein levels. The protein of Dectin-1 and TLR2 gradually increased till 16h. While pretreated with laminarin （a Dectin-1 inhibitor）, the expression of TLR2, CXCL1 and CXCL8 all decreased dramatically at the peak point, interestingly, when pretreated with TLR2 neutralizing antibody, the expression of Dectin -1, CXCL1 and CXCL8 also decreased dramatically at the peak point. CONCLUSION： These findings suggest that Dectin-1 and TLR2 co-regulated with each other after treated with inactive Af hyphae in the THCE cells, and they contribute together to the inflammatory responses by induction of chemokines CXCL1 and CXCL8.

关键词： dendritic cell-associated C-type lectin-1 corneal epithelial cell Toll-like receptor 2 chemokines

Characteristics of ocular abnormalities in gout patients

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International Journal of Ophthalmology(English edition) 2013年第3期6卷 307-311页

作者： Jing Lin gui-qiu zhao Cheng-Ye Che Shan-Shan Yang Qian Wang Chang-gui Li Department of Ophthalmology the Affiliated Hospital of Medical College of Qingdao University

·AIM: To characterize the clinical features of ocular surface in gout patients in coastal area of Shandong Province in China. ·METHODS: A total of 380 consecutive gout patients were examined from January 2011 to May 2011. According to the course of gout, patients were divided into group A (10 years). Group D (control group) was consist of 50 healthy subjects. Eyelids, lateral canthus, medial canthus, palpebral conjunctiva, sclera and cornea, anterior chamber, lens, anterior vitreous were examined by slit lamp to find whether there were deposition of uric acid crystals, ocular vascular tortuosity, redness and subconjunctival hemorrhage. The ophthalmic exams of visual acuity, intraocular pressure, fundus were used to assess any gout-related eye disease. ·RESULTS: Uric acid crystals were found in 3 patients and the positions of the deposite were in corneal stroma, corneal epithelium and superficial stroma, and sclera respectively. The incidence was 0.79% . Dilatated and tortuous blood vessels in conjunctiva and sclera surface were found in 38 (23.8% ), 40 (44.0% ), 58 (45.0% ), 9 (18.0% ) patients in groups A, B, C and D, respectively. The differences between group B and D, group C and D were statistically significant( <0.01, <0.01).Transparent vesicles with metal -like reflected light in subconjunctiva were seen in 26 (16.2%), 29 (31.9%), 41 (31.8%), 2 (4.00%) patients in groups A, B, C and D, respectively. The differences between A and D, B and D, C and D were statistically significant ( <0.05, <0.01, <0.01). Subconjunctival hemorrhage was found in all groups, the difference among the four groups showed no statistically significance. · CONCLUSION: Gout can cause ocular surface abnormalities, such as tophi deposition, subconjunctival transparent vesicles and hemorrhage, and vascular changes. These features have important clinical significance in early detection of the gout and prevention of eye injury. ·

关键词： ocular abnormalities gout tophi

Expression and significance of factors related to angiogenesis in choroidal melanoma

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International Journal of Ophthalmology(English edition) 2011年第1期4卷 49-54页

作者： Qiang Xu gui-qiu zhao Jie zhao Hong Lin Ying-Ying Mou Qing Wang and Wei-Rong Sun Department of Ophthalmology Affiliated Hospital of Qingdao University Medical Collage Qingdao 266003 Shandong Province China

AIM: To investigate expression of factors related to angiogenesis: HIF-1 alpha, iNOS, COX-2 and VEGF in choroidal melanoma and its clinical significance. METHODS: Fifty samples of choroidal melanoma and 15 samples of melanocytic nevi of the eyelid identified by pathology were collected. Immunohistochemistry SP method was used to examine the expression of HIF-1 alpha, iNOS, COX-2 and VEGF in these samples. The comparison among groups was done by SPSS 13.0 software. RESULTS: The positive expression rates of HIF-1 alpha, iNOS, COX-2 and VEGF in choroidal melanoma group were significantly higher than those in eyelid nevi group (chi(2)= 6.5542, 7.7224, 8.5828, 15.1749). The positive expression rate of VEGF was associated with the tumor size (chi(2)= 10.9194), but was not associated with pathological type (chi(2)=2.0712) and the situation of scleral invasion (chi(2)= 5.4289). The positive expression rate of HIF-1 alpha was associated with the tumor size (chi(2)=7.1216) and pathological type (chi(2)=9.0889), but was not associated with the situation of scleral invasion (chi(2)=3.3586). The positive expression rate of iNOS was associated with the tumor size (chi(2)=9.5503), but was not associated with pathological type (chi(2)=1.9450) and the situation of scleral invasion (chi(2)=2.3810). The positive expression rate of COX-2 was associated with the tumor size (chi(2)=7.2970), but was not associated with pathological type (chi(2)=1.8421) and the situation of sclera! invasion (chi(2)= 0.4018). The expression of HIF-1 alpha, iNOS and COX-2 were significantly associated with the expression of VEGF (r = 0.9429, 1, 0.9857). The expression of COX-2 was significantly associated with the expression of iNOS (r=0.9857). The expression of HIF-1 alpha was significantly associated with the expression of COX-2 ( r=0.9857). The expression of HIF-1 alpha was significantly associated with the expression of iNOS (r= 0. 9429). CONCLUSION: The expression of HIF-1 alpha, iNOS and COX-2 protein

关键词： choroidal melanoma immunohistoehemistry hypoxia inducible factor-1a inducible nitric oxide synthase cyclooxygenase-2

Protein tyrosine phosphatase 1B regulates migration of ARPE-19 cells through EGFR/ERK signaling pathway

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International Journal of Ophthalmology(English edition) 2015年第5期8卷 891-897页

作者： zhao-Dong Du Li-Ting Hu gui-qiu zhao Qian Wang Qiang Xu Nan Jiang Jing Lin Department of Ophthalmology Affiliated Hospital of Qingdao University

AIMTo evaluate whether protein tyrosine phosphatase 1B (PTP1B) contributed to initiate human retinal pigment epithelium cells (A)-19 migration and investigate the signaling pathways involved in this ***-19 cells were cultured and treated with the siRNA-PTP1B. Expression of PTP1B was confirmed by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). AG1478 [a selective inhibitor of epidermal growth factor receptor (EGFR)] and PD98059 (a specific inhibitor of the activation of mitogen-activated protein kinase) were used to help to determine the PTP1B signaling mechanism. Western blot analysis verified expression of EGFR and extracellular signal-regulated kinase (ERK) in ARPE-19 cells. The effect of siRNA-PTP1B on cell differentiation was confirmed by immunostaining for α-smooth muscle actin (α-SMA) and qRT-PCR. Cell migration ability was analyzed by transwell chamber *** mRNA levels of PTP1B were reduced by siRNA-PTP1B as determined by qRT-PCR assay. SiRNA-PTP1B activated EGFR and ERK phosphorylation. α-SMA staining and qRT-PCR assay demonstrated that siRNA-PTP1B induced retinal pigment epithelium (RPE) cells to differentiate toward better contractility and motility. Transwell chamber assay proved that PTP1B inhibition improved migration activity of RPE cells. Treatment with AG1478 and PD98059 abolished siRNA-PTP1B-induced activation of EGFR and ERK, α-SMA expression and cell ***1B inhibition promoted myofibroblast differentiation and migration of ARPE-19 cells, and EGFR/ERK signaling pathway played important role in migration process.

关键词： protein tyrosine phosphatase 1B retinal pigment epithelium cell migration epidermal growth factor receptor extracellular signal-regulated kinase

The expressions of metadherin and LEF-1 in mucosa-associated lymphoid tissue lymphoma of ocular adnexal

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International Journal of Ophthalmology(English edition) 2017年第5期10卷 705-710页

作者： Dan Qi Hong Lin Yan Gao Jing Lin Li-Ting Hu gui-qiu zhao Department of Ophthalmology the Affiliated Hospital of Qingdao University Qingdao 266003 Shandong ProvinceChina

AIM：To investigate the expressions of metadherin（astrocyte elevated gene-1, AEG-1） and lymphoid enhancerbinding factor-1（LEF-1） in ocular adnexal mucosaassociated lymphoid tissue（MALT） ***：The expressions of AEG-1 and LEF-1 were detected on specimens harvested from patients suffering from MALT lymphoma and lymphadenosis of ocular adnexal in Ophthalmology Department, Affiliated Hospital of Qingdao University from 2000 to 2015 by immunohistochemical and polymerase chain reaction（PCR） ***：AEG-1 and LEF-1 expressions in MALT lymphoma was respectively higher than that in lymphadenosis, both by immunohistochemical and PCR analysis（P〈0.05）. Diversity of AEG-1 and LEF-1 expressions in different Ann Arbor clinical stages showed a statistically significant result（P〈0.05）. A positive relevance between AEG-1 and LEF-1 was observed in MALT ocular adnexal lymphoma（r=0.435, P=0.016）.CONCLUSION：The over expressions of AEG-1 and LEF-1 at the level of protein and mR NA participates in the tumorigenesis of ocular adnexal MALT lymphoma. They should act as a new biological marker for pathological diagnosis in the future.

关键词： ocular adnexal lymphoma astrocyte elevatedgene- 1 lymphoid enhancer-binding factor- 1

Epidemiological characteristics and risk factors of diabetic retinopathy in type 2 diabetes mellitus in Shandong Peninsula of China

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International Journal of Ophthalmology(English edition) 2011年第2期4卷 202-206页

作者： zhao-Dong Du Li-Ting Hu gui-qiu zhao Yan Ma Zhan-Yu Zhou and Tao Jiang Department of Ophthalmology Affiliated Hospital of Medical College of Qingdao University Qingdao 266003 Shandong Province China

AIM: To determine the epidemiological characteristics and estimate the risk factors of diabetic retinopathy (DR) in patients with type 2 diabetes mellitus (T2DM) in Shandong Peninsula of China. METHODS: The cases of T2DM admitted to Affiliated Hospital of Medical College of Qingdao University, Shandong Province, China, from January 2006 to December 2010 were retrospectively reviewed. The epidemiological characteristics of DR were estimated. The cases were divided into two groups according to degrees of retinopathy: non-DR group and DR group. Logistic regression analysis was used to study the related risk factors of DR. RESULTS: The prevalence of DR in patients with T2DM was 25.08% (834/3326). There was significant difference between the average age for men (59.08 +/- 15.43 years) and for women (62.92 +/- 18.19 years, P=0.0021). The majority of DR occurred in women (female: male ratio=1.76:1, P<0.0001). The incidence rate of DR in urban (489/834) was higher than that in rural area (345/834, P<0.0001). In 834 DR patients, the mean duration of T2DM was 8.90 +/- 4.15 years (range: 0-16 years); 440 people (52.76%) had received varying degrees of health education about prevention and primary care of DM; and 473 people (56.71%) suffered from other DM complications confirmed at the same time. In addition, the incidence rate of monocular (551/3326) and binocular retinopathy (283/3326) were statistically different (P<0.0001). Factors associated (p<0.05) with the presence of DR included old age, lower health educational level, intraocular surgery history, longer duration of T2DM, accompanying with other DM complications, no standard treatment procedure, lower body mass index (BMI) and higher fasting plasma glucose (FPG), glycated hemoglobin A(1)C (HbA(1)C), urine albumin (UA), total cholesterol (TC), low-density-lipoprotein cholesterol (LDL-C). The risk factors (P<0.05) independently associated with the presence of DR were: longer duration of T2DM, lower health educational lev

关键词： diabetic retinopathy type 2 diabetes mellitus risk factors