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氨基葡萄糖/DNA复合纳米材料的构建及其对Raw264.7细胞功能的影响

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陆军军医大学学报 2024年第13期46卷 1494-1501页

作者：徐宇航陈跃向青张硕辛龚道辉吴迪王关嵩钱航陆军军医大学(第三军医大学)第二附属医院呼吸与危重症医学科重庆400037

目的构建一种无镁离子组装的新型氨基葡萄糖/DNA复合纳米材料,验证氨基葡萄糖能否介导组装DNA纳米管(nanotube,NT),并评价其对体外Raw264.7细胞功能的影响。方法采用梯度退火法,使用Y1、Y2和Y3这3条DNA单链,以氨基葡萄糖(glucosamine,Gl... 详细信息

目的构建一种无镁离子组装的新型氨基葡萄糖/DNA复合纳米材料,验证氨基葡萄糖能否介导组装DNA纳米管(nanotube,NT),并评价其对体外Raw264.7细胞功能的影响。方法采用梯度退火法,使用Y1、Y2和Y3这3条DNA单链,以氨基葡萄糖(glucosamine,GlcN)为介质组装DNA NT,构建氨基葡萄糖/DNA复合纳米材料(NT^(GlcN))。使用原子力显微镜(atomic force microscopy,AFM)观察其纳米结构,激光动态光散射(dynamic light scattering,DLS)测量该纳米材料的尺寸。选用Raw264.7细胞系,采用CCK-8法测试氨基葡萄糖或NT^(GlcN)的细胞毒性,流式细胞术和激光共聚焦考察细胞对该纳米结构的摄取效率,RT-qPCR测定NT^(GlcN)和NTMg(镁离子介导组装的DNA纳米管)对脂多糖(lipopolysaccharides,LPS)诱导的巨噬细胞炎症因子(IL-1β、IL-6)表达水平的影响。结果氨基葡萄糖可以成功介导NT^(GlcN)的合成,稳定性较好。AFM表征结果表明,NT^(GlcN)为管状颗粒,均匀地分散在云母表面上;DLS测量表明NT^(GlcN)的直径为(15.26±3.86)nm。细胞实验显示:较之NTMg,巨噬细胞对NT^(GlcN)有更高的细胞摄取效率,NT^(GlcN)处理后细胞存活率更高(P<0.05);NT^(GlcN)处理后,LPS诱导的巨噬细胞炎症因子表达降低(P<0.05)。结论本研究构建的氨基葡萄糖/DNA复合纳米材料具有良好的稳定性、生物相容性和细胞摄取效率;能降低氨基葡萄糖的细胞毒性,并能通过降低Raw 264.7细胞炎症因子的表达来抑制细胞炎症反应。

关键词： DNA纳米结构氨基葡萄糖巨噬细胞

Functionalizing DNA nanostructures with natural cationic amino acids

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Bioactive Materials 2021年第9期6卷 2946-2955页

作者： Dong wang Chunfa Chen Qian Liu Qianwen Zhao Di Wu Yue Yuan Chaowang Huang Xiaorong Sun Chunji Huang David Tai Leong guansong wang Hang Qian Institute of Respiratory Diseases Xinqiao HospitalThird Military Medical University183 Xinqiao StreetChongqing400037China Laboratory of Pharmacy and Chemistry And Laboratory of Tissue and Cell BiologyLab Teaching&Management CenterChongqing Medical UniversityChongqing400016China Basic Medical College Third Military Medical UniversityChongqing400038China Department of Chemical and Biomolecular Engineering National University of SingaporeSingapore Department of Respiratory Medicine Jinling HospitalNanjingJiangsu210002China

Complexing self-assembled DNA nanostructures with various functional guest species is the key to unlocking new and exciting biomedical *** guest species not only induce magnesium-free DNA to self-assemble into defined structures but also endow the final complex nanomaterials with new ***,we propose a novel strategy that employs naturally occurring cationic amino acids to induce DNA self-assembly into defined *** L-arginine and L-lysine can readily induce the assembly of tile-based DNA nanotubes and DNA origami sheets in a magnesium-free *** self-assembly processes are demonstrated to be pH-and concentration-dependent and are achieved at constant ***,the assembled DNA/amino acid complex nanomaterials are stable at a physiological temperature of 37◦*** L-arginine with its D form enhances its serum *** preliminary examination of this complex nanomaterial platform for biomedical applications indicates that DNA/amino acids exhibit distinct cellular uptake behaviors compared with their magnesium-assembled *** nanomaterial mainly clusters around the cell membrane and might be utilized to manipulate molecular events on the *** study suggests that the properties of DNA nanostructures can be tuned by complexing them with customized guest molecules for a designed *** strategy proposed herein might be promising to advance the biomedical applications of DNA nanostructures.

关键词： DNA nanostructures Noncanonical DNA self-Assembly Amino acids Isothermal self-assembly

Construction of core@double-shell structured energetic composites with simultaneously enhanced thermal stability and safety performance

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Defence Technology（防务技术） 2024年第4期34卷 134-142页

作者： Peng wang Wen Qian Ruolei Zhong Fangfang He Xin Li Jie Chen Li Meng Yinshuang Sun guansong He Institute of Chemical Materials China Academy of Engineering PhysicsMianyang 621900China State Key Laboratory of Environment-friendly Energy Materials School of Materials and ChemistrySouthwest University of Science and TechnologyMianyang 621010China

The poor thermal stability and high sensitivity severely hinder the practical application of hexanitrohexaazaisowurtzitane(CL-20).Herein,a kind of novel core@double-shell CL-20 based energetic composites were fabricated to address the above *** coordination complexes which consist of natural polyphenol tannic acid(TA) and Fe~Ⅲ were chosen to construct the inner shell,while the graphene sheets were used to build the outer *** resulting CL-20/TA-Fe~Ⅲ/graphene composites exhibited simultaneously improved thermal stability and safety performance with only 1 wt% double-shell content,which should be ascribed to the intense physical encapsulation effect from inner shell combined with the desensitization effect of carbon nano-materials from outer *** phase transition(ε to γ) temperature increased from 173.70 ℃ of pure CL-20 to 191.87℃ of CL-20/TA-Fe~Ⅲ/graphene ***,the characteristic drop height(H_(50)) dramatically increased from 14.7 cm of pure CL-20 to112.8 cm of CL-20/TA-Fe~Ⅲ/graphene composites,indicating much superior safety performance after the construction of the double-shell *** general,this work has provided an effective and versatile strategy to conquer the thermal stability and safety issues of CL-20 and contributes to the future application of high energy density energetic materials.

关键词： CL-20 Double-shell structure Thermal stability Safety performance Tannic acid Graphene sheets

Metformin inhibits dedifferentiation and proliferation of rat pulmonary arterial smooth muscle cells under hypoxia partly in AMPK-dependent way

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Metformin inhibits dedifferentiation and proliferation of ra...

Regular Observation of De-Acclimatization and Randomized Controlled Research of Diagnostic Criteria of High Altitude De-Acclimatization Syndrome among Different Plateau Migrants Crowd after Their Return to the Plain

中华医学会呼吸病学年会——2013（第十四次全国呼吸病学学术会议）

作者： Li Bai Zubin Yu Hongbo Tu Yafei Li Guisheng Qian guansong wang Department of Respiratory Disease Xinqiao HospitalThird Military Medical University Department of Thoracic Surgery Xinqiao HospitalThird Military Medical University Department of Epidemiology Third Military Medical University

Background and purposes The biguanide drug Metformin has been widely used as antidiabetic drug for treatment of hyperglycemia in patients with type 2 diabetes mellitus but has been recently shown to inhibit hypoxia-induced pulmonary remodeling and pulmonary hypertension(PH).However,the underlying mechanism remains poorly *** previous study has demonstrated the acquisition of proliferative activity of pulmonary arterial smooth muscle cells(PASMCs)

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Occupational Diseases and Environmental Medicine 2014年第4期2卷 86-100页

作者： Qiquan Zhou Shengyue Yang Zhencai Yuan Yinhu wang Xuefeng Zhang Wei Gao Zifu Shi Youli Yang Yunhong Wu Yong Fan Fuling wang guansong wang Department of High Altitude Disease College of High Altitude Military Medicine Third Military Medical University Chongqing China Key Laboratory of High Altitude Medicine of Ministry of Education and Key Laboratory of High Altitude Medicine of PLA Chongqing China Department of Respiratory Medicine Fourth Hospital of Chinese PLA Xining China Xianyang Hospital of China Railway First Group Co. Ltd. Xianyang China Research Institute of High Altitude Diseases 18th Hospital of Chinese PLA Yecheng China Research Center of High Altitude Medicine People’s Hospital of Golmud City Golmud China Department of Thoracic Surgery Lanzhou General Hospital of Lanzhou Command Lanzhou China The 68303 Troop Hospital of Chinese PLA Wuwei China Department of Clinical Laboratory Tenth Hospital of Chinese PLA Wuwei China Department of Endocrinology Hospital of Chengdu Office of Tibet Autonomous Region Chengdu China Research Institute of Respiratory Diseases Xinqiao Hospital Third Military Medical University Chongqing China

Objective: The objective of this study was to investigate the diagnostic methods of high altitude de-acclimatization syndrome and to formulate diagnostic criteria. Methods: This study was conducted using epidemiological surveys and a multi-center randomized controlled clinical trial. A total of 3011 subjects were studied, and the following indices were collected after their return to low altitude areas from the plateau: general health status, blood, urine and stool samples, myo-cardial enzyme levels, liver and kidney function, nerve function, sex hormone levels, microalbuminuria, electrocardiogram (ECG), echocardiography, pulmonary function, and hemorheological markers. These data were compared to those of randomized healthy subjects in the same age range who lived at the same altitude to determine the characteristics of high altitude de-acclimatization syndrome. Based on these characteristics, diagnostic criteria for high altitude de-acclimatization syndrome were formulated. Results: This study demonstrated that the incidence of high altitude de-acclimatization syndrome was 84.36%. Sixty percent of the cases were mild, 30% were medium, and 10% were severe. The incidence was higher among those who returned to a place of lower altitude, resided at a high altitude for a longer period of time, or engaged in heavy labor while at high altitude. Patients with high altitude de-acclimatization syndrome manifested hematological abnormalities and abnormal ventricular function, notably a right ventricular diastolic function, which recovered to baseline function after one to five years. Exposure to long-term hypoxia often caused obvious changes in cardiac morphology, i.e., left and right ventricular hypertrophy, particularly within the right ventricle. In addition, patients with high altitude de-acclimatization syndrome often presented with low blood pressure, low pulse pressure, and microalbuminuria. A few patients presented with occult blood in their feces. The diagnosis of h

关键词： Plateau Migrants Low Altitude High Altitude De-Acclimatization Syndrome Diagnostic Criteria Multi-Center Study

甲基-β-环糊精对肺泡Ⅱ型上皮细胞增殖和TGF-β/Smad信号通路的影响

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第三军医大学学报 2011年第8期33卷 780-784页

作者：王勤王建春李玉英王关嵩第三军医大学新桥医院全军呼吸内科研究所全军呼吸病研究重点实验室重庆400037

目的观察甲基-β-环糊精(methyl-β-cyclodextrin,MβCD)破坏小窝(caveolae)对肺泡Ⅱ型上皮细胞(AECⅡ)增殖和TGF-β/Smad信号通路的影响。方法分离培养大鼠AECⅡ,免疫荧光双标检测小窝蛋白-1(caveolin-1)和TGF-β受体Ⅰ(TβR-Ⅰ)表达... 详细信息

目的观察甲基-β-环糊精(methyl-β-cyclodextrin,MβCD)破坏小窝(caveolae)对肺泡Ⅱ型上皮细胞(AECⅡ)增殖和TGF-β/Smad信号通路的影响。方法分离培养大鼠AECⅡ,免疫荧光双标检测小窝蛋白-1(caveolin-1)和TGF-β受体Ⅰ(TβR-Ⅰ)表达和分布关系。以MβCD(5 mmol/L)破坏AECⅡ细胞膜Caveolae,设空白对照组,非离子去污剂法提取脂筏检测TβR-Ⅰ与Caveolin-1在细胞膜上的分布;Western blot检测Caveolin-1和磷酸化Smad2(pSmad2)表达;四甲基偶氮唑盐(MTT)法检测细胞增殖能力。结果荧光双标和脂筏提取结果提示TβR-Ⅰ主要分布于Caveolae区域,MβCD破坏Caveolae后,TβR-Ⅰ重新分布于细胞膜上非脂筏区域;MβCD干扰组Caveolin-1蛋白表达(24.53±3.24)%较正常对照组(54.83±5.67)%显著下调(P<0.01),而TGF-β/Smad信号通路下游分子pSmad2蛋白表达(10.93±1.11)%较对照组(8.36±0.64)%上调(P<0.05);MβCD干扰组细胞增殖率(31.00±4.18)%较对照组(49.20±4.44)%显著降低(P<0.01)。结论 MβCD破坏Caveolae结构可抑制AECⅡ增殖,其机制可能与TβR-Ⅰ在非脂筏区域的聚集和Caveolin-1下调导致的TGF-β/Smad信号通路增强有关。

关键词：肺泡Ⅱ型上皮细胞小窝小窝蛋白-1 转化生长因子β

SN50对脂多糖作用下小鼠Ana-1细胞TNFα表达的抑制作用的研究

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中国现代医学杂志 2009年第16期19卷 2409-2412,2416页

作者：肖波王建春王关嵩徐静第三军医大学新桥医院呼吸内科研究所重庆400037

目的研究核因子κB(NF-κB)抑制剂SN50对脂多糖(LPS)作用下小鼠巨噬细胞(Ana-1)炎性因子TNFαmRNA和蛋白表达的影响及其对NF-κBp65亚基核移位的抑制作用。方法用MTT法检测SN50对细胞增殖活性的影响,行RT-PCR和ELISA法检测巨噬细胞经LP... 详细信息

目的研究核因子κB(NF-κB)抑制剂SN50对脂多糖(LPS)作用下小鼠巨噬细胞(Ana-1)炎性因子TNFαmRNA和蛋白表达的影响及其对NF-κBp65亚基核移位的抑制作用。方法用MTT法检测SN50对细胞增殖活性的影响,行RT-PCR和ELISA法检测巨噬细胞经LPS作用和SN50处理后TNFαm-RNA和蛋白表达变化,用Western-Blot检测NF-κBp65亚基核移位情况。结果在LPS作用下炎性因子TNFα强烈升高,并呈现时间依赖性,SN50干预后可明显抑制NF-κBp65亚基核移位和TNFα表达,其中以早期使用SN50效果最佳。结论SN50可以通过抑制NF-κBp65亚基核移位来降低LPS作用下巨噬细胞的TNFα的产生。

关键词： SN50 巨噬细胞脂多糖 TNFα NF—κB

社会主义在当代中国的理论创新:理论工作者的使命与责任

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长江论坛 2006年第1期 94-95页

作者：王观松中共武汉市委党校

回首150多年马克思主义的发展历程,科学社会主义由理论变成现实,社会主义从一国发展推进到多国,国际共产主义运动经历过高潮与低潮交替,胜利与挫折并存,从而使科学社会主义的发展呈现波澜起伏、蜿蜒曲折的历史图景.马克思主义之所以能... 详细信息

回首150多年马克思主义的发展历程,科学社会主义由理论变成现实,社会主义从一国发展推进到多国,国际共产主义运动经历过高潮与低潮交替,胜利与挫折并存,从而使科学社会主义的发展呈现波澜起伏、蜿蜒曲折的历史图景.马克思主义之所以能够以强大的生命力发展到今天,科学社会主义之所以能够开拓出崭新的局面而生机盎然,其原因固然很多,但是有一点是不可忽视的,那就是马克思主义的坚定信仰者、科学社会主义的勇敢实践与开拓者们一代又一代人的连续不断与时俱进的理论创新,即对科学社会主义理论的不断发展与创新,这正是科学社会主义事业走向胜利、兴旺发达的重要前提与根本原因.

关键词：科学社会主义理论理论创新理论工作者当代中国国际共产主义运动马克思主义责任社会主义事业发展与创新与时俱进

受体相互作用蛋白140在脓毒症急性肺损伤大鼠肺泡巨噬细胞中的表达及其作用

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第三军医大学学报 2015年第9期37卷 851-857页

作者：雷传江焦艳王关嵩戢福云王建春第三军医大学新桥医院全军呼吸内科研究所全军呼吸病研究重点实验室重庆400037

目的探讨受体相互作用蛋白140（receptor interacting protein140,RIP140）在脓毒症急性肺损伤（acute lung injury,ALI）大鼠肺泡巨噬细胞中的表达及其在炎症反应过程中的作用。方法 36只雄性SD大鼠按随机数字表法分为正常对照组、假... 详细信息

目的探讨受体相互作用蛋白140（receptor interacting protein140,RIP140）在脓毒症急性肺损伤（acute lung injury,ALI）大鼠肺泡巨噬细胞中的表达及其在炎症反应过程中的作用。方法 36只雄性SD大鼠按随机数字表法分为正常对照组、假手术组和盲肠结扎穿孔术（CLP）致脓毒症肺损伤3、6、12、24 h组。HE染色观察肺组织病理变化;免疫组化检测RIP140在肺组织的表达分布;Western blot及免疫荧光观察肺泡巨噬细胞RIP140的表达变化及定位;RT-q PCR测定肺泡巨噬细胞IL-1β、TNF-α、IL-6 mRNA水平变化。结果 CLP组可见明显肺损伤病理改变且随时间进展逐渐加重。RIP140在脓毒症肺损伤大鼠肺组织中主要表达于炎性渗出细胞,且随时间进展,表达RIP140的细胞数量逐渐增多。CLP术后6 h大鼠肺巨噬细胞RIP140蛋白表达水平较正常对照组开始升高（P〈0.05）,至12~24 h维持一较高水平,且主要表达于细胞核。CLP术后3 h大鼠肺巨噬细胞IL-1β、TNF-α、IL-6 mRNA水平较正常对照组开始升高（P〈0.05）,至12~24 h维持一较高水平,下调肺泡巨噬细胞RIP140的表达可明显抑制IL-1β、TNF-α、IL-6 mRNA的表达。结论脓毒症肺损伤大鼠肺泡巨噬细胞可通过上调RIP140表达增强肺组织炎症反应,从而参与早期急性肺损伤的炎症应答。

关键词：受体相互作用蛋白140 急性肺损伤肺泡巨噬细胞脓毒症