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Alterations in DNA methylation and genome structure in two rice mutant lines induced by high pressure

Science china(Life sciences) 2006年第2期49卷 97-104页

作者： SHEN Sile1,3, WANG Zhenwei3, SHAN Xiaohui2, WANG Hua3, LI Ling3, LIN Xuyun2, LONG Likun2, WENG Kenan4. LIU Bao2 & ZOU Guangtian1 1. National Laboratory of Superhard Materials, Jilin University, Changchun 130012, china 2. institute of Genetics and Cytology, Northeast Normal University, Changchun 130024. china 3. College of Life Science, Jilin University, Changchun 130012, china 4. {4., chinese Academy of sciences, guangzhou 51064., china 1. National Laboratory of Superhard Materials Jilin University Changchun 130012 China 3. College of Life Science Jilin University Changchun 130012 China 2. institute of Genetics and Cytology Northeast Normal University Changchun 130024 China 4. guangzhou institute of geochemistry Chinese Academy of Sciences Guangzhou 510640 China

By using high-pressure treatment, two mutant lines were obtained from agenetically stable japonica rice cultivar Bijing38. Genomic DNA of the mutant lines, together withthe original line (Bijing38), was either undigested or digested by Hpa ⅡIMspⅠ, and then subjectedto molecular analysis using two markers, ISSR and RAPD. Results indicated that changes in the PCRamplification profiles of both markers are apparent in the two mutant lines compared with theoriginal rice cultivar, suggesting that there had been both sequence changes and DNA methylationmodifications in the mutant lines. Southern blot analysis using diverse sequences, including twocellular genes (S2 and S3), a set of retrotransposons (Osr7, Osr36, Tos19 and more), and a MITEtransposon family (mPing and Pong), confirmed the results, and indicated that changes in DNAmethylation pattern, genomic structure, and possible activation of some transposons indeed occurredin the mutant lines. Moreover, these changes are stably maintained through selfed generations and indifferent organs. Thus, our results indicate that it is possible to obtain stable mutants in riceby high pressure treatments, and the molecular basis of the mutants may include both genetic andepigenetic changes. Therefore, high hydrostatic pressure seems a promising approach for plantmutagenesis.

关键词： DNA methylation, genomic changes, high pressure, mutagenesis, rice.

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The Dabieshan Coesite-bearing Eclogite Terrain-A Late Archaean Ultra - high- Pressure Metamorphic Belt

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Acta Geologica Sinica(English Edition) 1996年第1期70卷 46-58页

作者： Cao Ronglong and Zhu Shouhuaguangzhou institute of geochemistry, chinese Academy of sciences, guangzhou, Guangdong guangzhou institute of geochemistry Chinese Academy of Sciences Guangzhou Guangdong

A U -Pb zircon age of 2774.24.Ma for eclogite from the Bixiling rock body of Anhui Province, central china, indicates that the Dabieshan coesite-bearing eclogite was probably formed in the Late Archaean. A phengite Ar-Ar isochron age of 662±13 Ma for the eclogite confines also an upper limit age of its subsequent retrograde metamorphism in the Precambrian. The results of isotopic dating for such type of eclogite coincide with the geological features of its restricted occurrence within the Archaean metamaorphic terrain composed of the Dabie Group. It is believed that the Dabieshan coesite-bearing eclogite terrain might be a Late Archaean ultra-high-pressure metamorphic belt. The Dabie Mountains area was the eastward extension of the southern Qinling structural belt during the Triassic. Both the Dabie Group and the coesite-bearing eclogite hosted therein underwent a late-stage dynamic metamorphic event. The present authors have obtained a muscovite Ar-Ar isochron age of 192.6±2.8 Ma from plagioclase gneiss and a hornblende Ar-Ar plateau age of 230.7±4.6 Ma for the low amphibolite in eclogite respectively, which represent the Indosinian reworking ages of the original metamorphic rocks of the Dabie Group gneiss and coesite-bearing eclogite.

关键词： Dabie Mountains coesite-bearing eclogite zircon U -Pb dating Late Archaean ultra-high-pressure metamorphic belt

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Experimental Study of Partial Melting of Mantle Peridotite -A Discussion about the Genesis of Sih'ca-rich Fluids (Melts)

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Acta Geologica Sinica(English Edition) 1999年第4期73卷 395-403页

作者： LI Jianping guangzhou institute of geochemistry, chinese Academy of sciences, guangzhou, {4., chinaand Kornprobst JACQUES Departement des sciences de la Terre, Universite Blaise Pascal, 610038 Clermont-Ferrand, France chinese Acad Sci Guangzhou Inst Geochem Guangzhou 510640 Peoples R China Univ Blaise Pascal Dept Sci Terre F-610038 Clermont Ferrand France

Experiments on partial melting of mantle lherzolite have been realized at 0.6 and 1.0 GPa and the chemical compositional variations of melts during different melting stages have been first discussed. The results show that the trends of variations in SiO2, CaO, Al2O3, Na2O and TiO2 are different at different melting stages. The melts produced at lower pressure are richer in SiO2 than those at higher pressure. The mantle-derived silica-rich fluids (silicate melts) are polygenetic, but the basic and intermediate-acid silicate melts in mantle peridotite xenoliths from the same host rocks, which have equivalent contents of volatile and alkali components and different contents of other components, should result from in-situ (low-degree) partial melting of mantle peridotite under different conditions (e.g. at different depths, with introduction of C-O-H fluids or in the presence of metasomatic minerals). The intermediate-acid melts may be the result of partial melting (at lower pressure) Opx + Sp + K-Na-rich fluid ±(Am-phi) ± (Phlog) = OI + melt. But the intermediate-acid magmas cannot be produced from the partial melting of normal mantle peridotite unless the crustal materials are introduced to some extent.

关键词： mantle peridotite in-situ partial melting compositional variation of melt genesis of silica-rich fluid (stlicate melt)

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Discovery of seep carbonate nodules as new evidence for gas venting on the northern continental slope of South china Sea

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chinese Science Bulletin 2006年第10期51卷 1228-1237页

作者： CHEN Zhong1,2,3, YAN Wen1,2,3, CHEN Muhong1,3, WANG Shuhong1,2,3, LU Jun1,3, ZHANG Fan1,3, XIANG Rong1,3, XIAO Shangbin1,3, YAN Pin1,2,3 & GU Senchang1,2,3 1. South china Sea institute of Oceanology, chinese Academy of Sci-ences, guangzhou 510301, china 2. guangzhou Center for Gas Hydrate Research, chinese Academy of sciences, guangzhou 510301, china 3. Key Laboratory of Marginal Sea Geology, South china Sea institute of Oceanology and guangzhou institute of geochemistry, chinese Academy of sciences, guangzhou 510301, china South china Sea institute of Oceanology Chinese Academy of Science Guangzhou China guangzhou Center for Gas Hydrate Research Chinese Academy of Sciences Guanzhou China Key Laboratory of Marginal Sea Geology South China Sea Institute of Oceanology and Guangzhou Institute of Geochemistry Chinese Academy of Sciences Guangzhou China

Seep carbonate nodules were firstlycollected from the southwestern Dongsha area onnorthern continental slope of South china Sea formineralogical and geochemical studies. The resultsof X-ray diffraction show that carbonate nodules arecomposed of Fe-rich domolite,siderite and a few cal-cite and aragonite, as well as a small quantity ofnon-carbonate minerals such as goethite, quartz andclay minerals. Fe-rich dolomite and siderite charac-terized by euhedral microcrystalline structure weredirectly precipitated at cold seeps. The δ 13C valuesfor Fe-rich dolomite and siderite in the bulk nodulesvary from ?18.24. to ?36.07‰, and the δ 18O valuesrange from 0.4. to 2.76‰. Their moderate depleted13C reflects that carbon origin is possibly thermoge- netic gas or mixed gas, which is evidence of gasventings in the seafloor. Moreover, massive wormtube fossils related to cold seeps were found on thesurfaces of carbonate nodules, and the conduitsand/or channels in semi-solidified nodules whichwere not filled by carbonate or sediment were alsoobserved. A preliminary conclusion is that activemicro gas venting with a conduit/channel diameterrange from 200 μm to 600 μm possibly exists inmodern seafloor of the carbonate nodule area.

关键词：渗出碳酸盐节碳氧同位素甲烷渗漏天然气水化物

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Experiment of the ^(4.)Ar/^(39)Ar dating of diagenetic illite in gas reservoirs

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chinese Science Bulletin 2004年第S1期49卷 89-93页

作者： WANG Longzhang, DAI Tongmo & PENG Ping’an State Key Laboratory of Organic geochemistry, guangzhou institute of geochemistry, CAS, guangzhou 51064., china Faculty of Resources, {4. University of Geosciences, Wuhan 4.0074. china 1. State Key Laboratory of Organic geochemistry Guangzhou Institute of Geochemistry CAS 510640 Guangzhou China 2. Faculty of Resources Chinese University of Geosciences 430074 Wuhan China

4.Ar/39Ar dating of diagenetic illite has been performed to investigate gas reservoirs in the Sulige Gas Field of the northern Ordos Basin. A series of technical challenges were confronted, including illite purification, Ar recoil loss, and separation of diagenetic illite from detrital illite. Mineral growth ages for diagenetic illite were obtained by this experiment, from which the age of gas emplacement was deduced to be later than 169 Ma.

关键词： 4.Ar/39Ar dating diagenetic illite purification of clay minerals Ar recoil loss time of gas emplacement.

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泮塘五约微改造,广州,广东,中国

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世界建筑 2024年第8期 58-63页

作者： Urban Elephant Architectural Design & Planning (guangzhou) Co.,Ltd. guangzhou Urban Renewal Planning & Design institute Co.,Ltd. 象城建筑规划设计(广州)有限公司广州市城市更新规划设计研究院

广州荔湾泮塘五约更新计划始于2011年底,至今仍在持续。本项目展示了2016-2024.间针对建筑和环境空间的历次设计、改造,反映了广州历史街区改造中出现的城市遗产整体观念,以及更新设计方法、修缮技术的不断尝试和逐渐进步。泮塘五约更... 详细信息

广州荔湾泮塘五约更新计划始于2011年底,至今仍在持续。本项目展示了2016-2024.间针对建筑和环境空间的历次设计、改造,反映了广州历史街区改造中出现的城市遗产整体观念,以及更新设计方法、修缮技术的不断尝试和逐渐进步。泮塘五约更新计划是在城市历史景观视角下,通过对建成遗产与环境、历史与当代生活记忆的阅读、理解,建立景观、遗产与传统文化的整体关联,并通过公众参与式的设计与共同缔造,实现多主体共同愿景下的保护、活化与发展。

关键词：项目展示城市历史景观整体观念广州荔湾更新设计公众参与泮塘共同愿景

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A preliminary analysis on lithospheric thickness and crustal ages in central basin of South china Sea

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Acta Oceanologica Sinica 1997年第1期16卷 85-107页

作者： Chen Xue and Lin Jinfeng South china Sea institute of Oceanology, the chinese Academy of sciences, {4. 510301, china South china Sea institute of Oceanology Chinese Academy of Sciences Guangzhou 510301 China

The models about lithospheric thickness and thermal conduction inside the lithosphere and the top layer of the asthenosphere have been proposed in this study for four type regions: the midoceanic ridge, the extinct spreading ridge, the lithospheric fault fissure and the mouth of the extinct submarine volcanoes which are in deep sea bottom. The solutions of the models are found to be the same. The formulas of temperature distribution inside the lithosphere and the top layer of the asthenosphere, the lithospheric thicknesses to the heat flow and the crustal ages to the heat flow are obtained. The crustal ages and the lithospheric thicknesses of the central basin are calculated. And they are used to draw the lithospheric thicknesses and crustal ages maps of the central basin (in this paper both the central basin and the basin are the central basin of the South china Sea). According to their characteristics, the central basin is divided into three regions. The lithospheric thicknesses, crustal ages and heat flow distribution characteristics are discussed respectively. The formation and evolution of the South china Sea are analysed and it is thought that the South china Sea has undergone three episode-seafloor spreadings.

关键词： Thermal conduction equation lithospheric thickness crustal age evolution

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ABUNDANCE AND DISTRIBUTION OF PLATINUM-GROUP ELEMENTS (PGE) IN PERIDOTITE FROM THE DAGZHUKA OPHIOLITE IN TIBET:IMPLICATIONS FOR MANTLE METASOMATISM

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地学前缘 2000年第S1期7卷 99-100页

作者： Xia Bing 1,2 ,Yu Hengxiang 1,Mei Houjun 1,Qi Lang 2 (1 guangzhou institute of geochemistry, chinese Academy of sciences, guangzhou 51064.,{4. 2 institute of geochemistry, chinese Academy of sciences, Guiyang 550002,{4.)

Information on abundance and distribution of platinum\|group elements (PGE) in peridotite from ophiolite is relevant to understand the nature and evolution of the upper mantle. The ophiolite suite outcropping along the famous suture zone of Yarlung Zangbo River, Tibet, has attracted wide attention of geologists both in china and abroad. The Dagzhuka ophiolite of in the suture zone is thought to display a complete ophiolitic sequence. The peridotite from the Dazhuka ophiolite is composed of fairly fresh or unaltered lherzolite, harzburgite and minor dunite which are the upper mantle residuum formed by 15%～35% partial melting. In this paper we have mainly studied the abundance and distribution of PGE in the harzburgites and lherzolites. The harzburgites and lherzolites preserve total PGE contents of 28 37×10 -9 ～50 67×10 -9 respectively higher than Primitive mantle or the peridotite from some typical ophiolites and Alpine. They all have fractionated chondrite\|normalized PGE patterns with positive slopes from Ir to Pd (Fig.1), and Pd/Ir=1 13～2 4.>1, Pd/Rh=2 23～7 4.>1, and Pd/Pt=0 26～1 16 (average 0 67) higher than Primitive mantle (1 11, 2 35, 0 57) or CI\|chondrite (1 01, 2 73, 0 53). Consequently, the Dagzhuka peridotite are PGE\|enriched, but otherwise possess residual characteristics arising from a minimum of 15% partial melting. It is suggested that mantle residuum by partial melting have low total PGE contents, fractionated chondrite\|normalized PGE patterns with negative or slightly flat slopes from Ir to Pd, and Pd/Ir<1. But, the total PGE contents, chondrite\|normalized PGE patterns and Pt/Ir, Pd/Rh and Pd/Pt values of the Dagzhuka peridotite are completely inconsistent with a residual origin. Partial melting would have partitioned all sulfide in the source into the melt. In fact, no sulfide or silicate melt remained in the Dagzhuka peridotite. Therefore, PGE in Dagzhuka peridotite are not present in sulfides. The PGE\|enrichment and fractionation of the Dagz

关键词： platinum\|group elements(PGE) peridotites Dazhuka ophioli te Tibet

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Lincang Superlarge Germanium Deposit in Yunnan Province,china: Sedimentation,Diagenesis,Hydrothermal Process and Mineralization

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Journal of Earth Science 1998年第2期17卷 37-44页

作者： Zhuang Hanping Lu Jialan Fu Jiamo Liu Jinzhong guangzhou institute of geochemistry, chinese Academy of sciences, guangzhou 51064.

The mineralization is related closely to sedimentation, diagenesis and hydrothermal processes. In this paper, investigations are carried out on coal occurrence, maceral composition, inorganic minerals, trace elements and huminite reflectance. It is concluded that the source of Lincang superlarge deposit is mainly the muscovite granite in the west edge of the basin. During sedimentation, Ge (germanium) was leached out and entered the basin. Ge was adsorbed by lower organism and humic substances in water. Lincang lignite underwent three thermal processes: peatification, early diagenesis and hydrothermal transformation. During peatification, Ge was adsorbed or complexed by humic colloids. During early diagenesis, the Ge associated with humic acids was hard to mobilize or transport. Most of Ge entered the structure of huminite while a small amount of Ge was associated with residual humic acids as complex or humate. During hydrothermal transformation, the heated natural water or deep fluid from basement encountered the coal layer within tectonic weak zone. SO 2- 4. was reduced by coal organic matter. Pyrite and calcite formed. Hydrothermal process did not contribute significantly to mineralization.

关键词： Lincang superlarge germanium deposit sedimentation diagenesis hydrothermal process mineralization.

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Discovery of Permian island-arc basalts in the Cenxi area and the tectonic significance──A piece of evidence for the paleo-Tethyan tectonic belt in western Guangdong and eastern Guangxi

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chinese Science Bulletin 1998年第21期43卷 1838-1842页

作者： ZHANG Boyou 1, ZHAO Zhenhua 1, SHI Manquan 1, YANG Shufeng 2 and CHEN Hanlin 2 1. guangzhou institute of geochemistry, chinese Academy of sciences, guangzhou 51064.,{4. 2. Department of Earth sciences, Zhejiang University, Hangzhou 310027,{4. guangzhou institute of geochemistry Chinese Academy of Sciences Guangzhou China Department of Earth sciences Zhejiang University Hangzhou China

Cenxi Permian basalts are the late Palaeozoic island-arc type basalts first found in South china. REE and trace element characteristics and distribution patterns show that this volcanic formation, which has been isotopically dated at 261 MaB.P. by the 39Ar/ 4.Ar method, is of an active continental marginal island arc type, and that the tectonic environment at that time was an island-arc marginal sea or minor ocean basin related with the paleo-Tethyan tectonic belt in western Guangdong-eastern Guangxi.

关键词： spilite-keratophyre pillow lava Permian continental margin paleo-Tethyan evolution.

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