检索结果-南通市图书馆

图书情报工作 2000年第2期44卷 68-71页

作者：邱瓞曾广州医学院图书馆广州510182

在简要回顾两年来该校图书馆的主要变化的基础上,探讨如何从理论与实践相结合的角度,运用组织发展理论推进内部管理体制改革。

关键词：管理心理学高等教育图书馆管理管理体制

Promoter polymorphism of MRP1 associated with reduced survival in hepatocellular carcinoma

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World Journal of Gastroenterology 2010年第48期16卷 6104-6110页

作者： Jing Zhao,Bing-Yun Yu,Jin-E Yang,Key Laboratory of Gene Engineering of the Ministry of Education,School of Life Sciences,Sun Yat-sen (Zhongshan) University,guangzhou 510275,Guangdong Province,China Dao-Yuan Wang,Department of medical Oncology,The First Affiliated Hospital of guangzhou medical college,guangzhou 510120,Guangdong Province,China Key Laboratory of Gene Engineering of the Ministry of Education School of Life Sciences Sun Yat-sen (Zhongshan) University Xin Gang Xi Road 135# Guangzhou 510275 Guangdong Province China. lssyje@***

AIM:To investigate the effect of the G-1666A polymorphism in the multidrug resistance related protein-1 (MRP1) on outcome of hepatocellular carcinoma (HCC). METHODS:A cohort of 162 patients with surgically resected HCC who received no postsurgical treatment until relapse was studied. Genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism analysis. Electrophoretic mobility shift assay (EMSA) was used to evaluate the influence of the G-1666A polymorphism on the binding affinity of the MRP1 promoter with its putative transcription factors. RESULTS:Kaplan-Meier analysis showed that patients with GG homologues had a reduced 4-year disease-free survival compared with those carrying at least one A allele (P = 0.011). Multivariate Cox regression analysis indicated that the-1666GG genotype represented an independent predictor of poorer disease-free survival [hazard ratio (HR) = 3.067,95% confidence interval (CI):1.587-5.952,P = 0.001],and this trend became worse in men (HR = 3.154,95% CI:1.604-6.201,P = 0.001). A similar association was also observed between 4-year overall survival and the polymorphism in men (HR = 3.342,95% CI:1.474-7.576,P = 0.004). Moreover,EMSA suggested that the G allele had a stronger binding affinity to nuclear proteins. CONCLUSION:The MRP1-1666GG genotype predicted a worse outcome and was an independent predictor of poor survival in patients with HCC from Southeast China.

关键词： Multidrug resistance related protein-1 Single nucleotide polymorphism Hepatocellular carcinoma Prognosis

Conservation-based prediction of the transcription regulatory region of the SCN1A gene

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Progress in Natural Science:Materials International 2009年第12期19卷 1675-1681页

作者： Yue-Sheng Long,Yi-Wu Shi,Wei-Ping Liao~* Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and The Ministry of Education of China,guangzhou 510260,China Institute of Neuroscience and The Second Affiliated Hospital of guangzhou medical college,guangzhou 510260,China [a]Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and The Ministry of Education of China Guangzhou 510260 China [b]Institute of Neuroscience and The Second Affiliated Hospital of guangzhou medical college Guangzhou 510260 China

A challenge in identifying the transcription regulatory region is that the locations of eukaryotic transcriptional elements are often diverse among different ***1A,a disease-related sodium channel gene,has a complex 5’-untranslated region and diverse mRNA transcripts,which might be driven by different *** cross-species sequence comparison and bioinformatics analysis,human 5’- untranslated exons were found to be conserved within the region of 200 kb upstream of the 5’ flanking regions of SCN1A in higher mammals, but not in lower mammals and *** core promoter elements(INR,DPE,and TATA) were found in the regions flanking different 5’-untranslated exons,suggesting that these sequences(from -45 to +35) might be targeted as core *** nucleotide identity rate of these core promoter sequences are different,and the conservation level of the upstream region of each core promoter varies distinctly,implicating different regulatory mechanisms of the four promoters which exist in the nervous system.

关键词： 5’-UTR Gene expression SCN1A Promoter

The Biological Study of the Cultured Human Lens Epithelial Cells in Vitro

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眼科学报 1994年第1期10卷 27-31页

作者： Haike Guo,Shaozhen Li, Xinyuan Cao, Shunwei LiangDepartment of Ophthalmology, The Second Teaching Hospital, guangzhou medical college guangzhou 510260, ChinaZhongshan Ophthalmic Center , Sun Yet-Sen University of medical Sciences guangzhou 510060, China Department of Ophthalmology Second Teaching Hospital Guangzhou Medical College China.

The human lens epithelial cells (HLE) cultured in vitro was established in normal and cataractous lenses. The biological feature, histological characteristics and the ultrastructure of the cultured HLE cells were investigated. The results reveal that the proliferative capacity of the culutured HLE cells is reversely proportional to the donour age; the cultured HLE cells has the limited proliferative capacity in vitro. The relieve of the contact inhibition is the effective trigger of the HLE cell prolife...

关键词： cell culture lens cataract

INHIBITION OF RAT LENS ALDOSE REDUCTASE BY QUERCETAGETIN AND PATULETIN

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眼科学报 1991年第1期7卷 29-30,33页

作者： Shaozhen Li Winifred Mao Xinyuan Cao Shunwei Liang Zhongshan Ophthalmic Center Sun Yat-sen University of medical Sciences guangzhou, China Zhou Ding Naiming Li Department of Chemistry guangzhou medical college guangzhou, China Zhongshan Ophthalmic Center Sun Yat-sen University of Medical Sciences Guangzhou China.

In this paper the results of inhibition of the Aldose reductase(AR) activity on Wistar rat lens by Quercetagetin extracted from Tagetes erects Linn and by Patuletin extracted from Tagetes patula Linn are *** inhibited AR of the rat lens by 93.9% at 10^(-4)M, 76.0% at 10^(-5)M and 13.3% at 10^(-6)M. Patuletin inhibited AR of the rat lens by 100% at 10^(-1)M, 80% at 10^(-5)M and 22.7% at 10^(-6)M respectively. The results show that these two flavones are lens AR Inhibitors, but further ...

关键词： INHIBITION OF RAT LENS ALDOSE REDUCTASE BY QUERCETAGETIN AND PATULETIN RAT AR

Efficacy of intramuscular BCG polysaccharide nucleotide on mild to moderate bronchial asthma accompanied with allergic rhinitis: a randomized, double blind, placebo-controlled study

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中华医学杂志(英文版) 2005年第19期000卷 13-21页

作者： LI Jing, LUO Ding-fen, LI Sui-ying, SUN Bao-qing and ZHONG Nan-shan guangzhou Institute of Respiratory Disease, First Affiliated Hospital of guangzhou medical college, guangzhou 510120, China guangzhou Institute of Respiratory Disease First Affiliated Hospital of Guangzhou Medical College Guangzhou 510120 China

The extensive evidence of Th2 cytokine involvement in the pathogenesis of allergic diseases~ 1,2 suggests a potential target for immunomodulation. Because the Th1 and Th2 cells are reciprocally regulated, several recent studies on Th2-associated diseases including asthma have shown to reverse the Th2 response into a protective Th1 response.~ 3,4

关键词： asthma · allergic rhinitis · Bacillus Calmette-Guérin polysaccharide nucleotide

Sequence Analysis and Structural Prediction of the Severe Acute Respiratory Syndrome Coronavirus nsp5

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Acta Biochimica et Biophysica Sinica 2005年第7期37卷 473-479页

作者： Jia-Hai LU Ding-Mei ZHANG Guo-Ling WANG Zhong-Min GUO Juan LI Bing-Yan TAN Li-Ping OU-YANG Wen-Hua LING Xin-Bing YU Nan-Shan ZHONG~1 The Public Health School of Sun Yat-Sen University,guangzhou 510080,China 1 guangzhou Institute of Respiratory Diseases,guangzhou medical college,guangzhou 510120,China The Public Health School of Sun Yat-Sen University Guangzhou 510080 China

The non-structural proteins (nsp or replicase proteins) of coronaviruses are relativelyconserved and can be effective targets for *** studies have been conducted into the function of thesevere acute respiratory syndrome coronavirus (SARS-CoV) *** this study,bioinformatics methodswere employed to predict the secondary structure and construct 3-D models of the SARS-CoV GD *** and sequential comparison was performed to analyze the mutation trend of the polymerasensp5 gene during the epidemic process using a nucleotide-nucleotide basic local alignment search tool(BLASTN) and a protein-protein basic local alignment search tool (BLASTP).The results indicated that thensp5 gene was steady during the epidemic process and the protein was homologous with other coronavirusnsp5 *** protein encoded by the nsp5 gene was expressed in COS-7 cells and analyzed by sodiumdodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE).This study provided the foundation forfurther exploration of the protein's biological function,and contributed to the search for anti-SARS-CoVdrugs.

关键词： severe acute respiratory syndrome (SARS) severe acute respiratory syndrome coronavirus (SARS-CoV) non-structural protein (nsp) replicase secondary structure 3-D structure

Cloning, expression, and characterization of pollen allergens from Humulus scandens (Lour) Merr and Ambrosia artemisiifolia L

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Acta Pharmacologica Sinica 2005年第10期26卷 1225-1232页

作者： Ai-lin TAO~(2,3), Shao-heng HE~(2,4) Allergy and Inflammation Research Institute, Shantou University medical college, Shantou 515031, China," ~The Second Affiliated Hospital of guangzhou medical college, guangzhou 510260, China Allergy and Inflammation Research Institute Shantou University Medical College Shantou 515031 China

Aim: To clone the pollen allergen genes in Humulus scandens (Lour) Merr (LuCao in Chinese) and short ragweed (Ambrosia artemisiifolia L) for recombinant allergen production and immunotherapy. Methods: The allergen genes were selectively amplified in the weed pollen cDNA pool by using a special PCR profile, with the primers designed by a modeling procedure. Following truncated gene cloning and confirmation of the pollen source, unknown 3’cDNA ends were identified by using the 3’-RACE method. The gene function conferred by the full-length coding region was evaluated by a homologue search in the GenBank database. Recombinant proteins expressed in Escherichia coli pET-44 RosettaBlue cells were subsequently characterized by N-terminal end sequencing, IgE binding, and crossreactivity. Results: Three full-length cDNAs were obtained in each weed. Multiple alignment analysis revealed that the deduced amino acid sequences were 83% identical to each other and 56%-90% identical to panallergen profilins from other species. Five recombinant proteins were abundantly expressed in nonfusion forms and were confirmed by using the N-terminal end sequence identity. Sera from patients who were allergic to A artemisiifolia reacted not only with rAmb a 8(D03) derived from A artemisiifolia, but also with recombinant protein rHum s 1(LCM9) derived from H scandens, which confirmed the allergenicity and cross-reactivity of the recombinant proteins from the 2 sources. Comparison of the degenerate primers used for truncated gene cloning with the full-length cDNA demonstrated that alternative nucleotide degeneracy occurred. Conclusion: This study demonstrates a useful method for cloning homologous allergen genes across different species, particularly for little-studied species. The recombinant allergens obtained might be useful for the immunotherapeutic treatment of H scandens and/or A artemisiifolia pollen allergies.

关键词： pollen allergen profilin gene expression

THE APPLICATION OF FIBRIN GLUE IN THORACIC ANDCARDIOVASCULAR SURGERY

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Chinese Journal of Biomedical Engineering(English Edition) 1997年第3期6卷 197-198页

作者： Zhang Bengu（Thoracic and Cardiovascular Surgery ofSecond Affiliated Hospital of guangzhou medical college）

Arsenious Acid Inducing Differentiation Of Hepatoma Stem Bel-7402

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Arsenious Acid Inducing Differentiation Of Hepatoma Stem Bel...