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Selective enrichment of hepatocytes from mouse embryonic stem cells with a culture system containing cholestatic serum

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Acta Pharmacologica Sinica 2007年第12期28卷 1931-1937页

作者： Jun MIN~(2,3),Chang-zhen SHANG~2,Ya-jin CHEN,Lei ZHANG,Lu LIU,Xiao-geng DENG,Mei YANG,Dong-ping CHEN,JunCAO,Er-wei SONG,Ji-sheng CHENDepartment of Hepatobiliary Surgery,The Second Affiliated Hospital of Sun Yat-sen University,guangzhou 510120,china Department of Hepatobiliary Surgery The Second Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510120China

Aim:There is increasing evidence indicating that embryonic stem (ES) cells arecapable of differentiating into hepatocyte-like cells in ***,it is neces-sary to improve the differentiation efficiency so as to promote the ***,we report an efficient culture system to support hepatocytedifferentiation from ES cells by utilizing cholestatic ***:One weekafter the induction of E14 mouse ES cells into hepatocytes with sodium butyrate,cholestatic serum was added into the culture system at various concentrationsand hepatocyte-like cells were induced to *** morphological andphenotypic markers of hepatocytes were characterized using light microscopy,immunocytochemistry,and RT-PCR,*** function of glycogen stor-age of the differentiated cells was detected by Periodic acid-Schiff (PAS) reaction,and the ratio of hepatic differentiation was determined by counting the albuminand PAS-positive ***:In the presence of conditional selective mediumcontaining cholestatic serum,numerous epithelial cells resembling hepatocyteswere *** RT-PCR analysis showed that undifferentiated ES cells didnot express any hepatic-specific markers;however,in the presence of sodiumbutyrate and conditional selective medium containing cholestatic serum,hepaticdifferentiation markers were *** staining showed thatthose ES-derived hepatocytes were α-fetoprotein,albumin,and cytokeratin 18positive,with the ability of storing *** determination of the hepaticdifferentiation ratio showed that the application of cholestatic serum efficientlyenriched ES-derived hepatocyte-like cells by inducing lineage differentiation andenhancing lineage ***:The conditional selective mediumcontaining cholestatic serum is optimal to selectively enrich hepatocyte-like cellsfrom mixed differentiated ES cells,which may provide a novel method to improvethe hepatic differentiation ratio of ES cells.

关键词： embryonic stem cells sodium butyrate hepatocyte cholestatic serum differentiation

骨髓增生异常综合征合并自身免疫性疾病5例分析

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中国实用内科杂志 2005年第2期25卷 152-153页

作者：赖琳詹俊刘建平中山大学附属第二医院消化内科广州510120

目的　探讨骨髓增生异常综合征 (MDS)合并自身免疫性疾病的特点。方法　对 1997- 0 1～ 2 0 0 2 -0 6中山大学附属第二医院消化科 6 0例MDS患者的临床资料进行分析和随访观察。结果　 6 0例MDS患者中 ,5例合并自身免疫性疾病 ,其中格雷... 详细信息

目的　探讨骨髓增生异常综合征 (MDS)合并自身免疫性疾病的特点。方法　对 1997- 0 1～ 2 0 0 2 -0 6中山大学附属第二医院消化科 6 0例MDS患者的临床资料进行分析和随访观察。结果　 6 0例MDS患者中 ,5例合并自身免疫性疾病 ,其中格雷夫斯病 3例 ;合并自身免疫性疾病患者MDS的分型以环形铁粒幼细胞性难治性贫血 (RAS)最多见 (4例 )。结论　MDS可以合并格雷夫斯病 ,而体液免疫异常可能是MDS合并自身免疫性疾病的一个重要环节。FAB分型与是否合并自身免疫性疾病之间可能并无相关。

关键词：自身免疫性疾病 MDS 骨髓增生异常综合征患者格雷夫斯病随访观察 FAB分型合并目的重要环节

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恙虫病113例临床分析

中国实用内科杂志 2006年第S2期26卷 29-30页

作者：冯素玲罗泽如李建国中山大学附属第二医院呼吸内科广东广州510120 广东省人民医院东病区呼吸内科广东广州510080

目的探讨恙虫病的临床特点。方法对1991—2005年收治的113例恙虫病病例进行回顾性分析。结果发热占100%,焦痂或溃疡占80.5%(91/113),皮疹占6.2%(7/113),淋巴结肿大占75.2%(85/113),合并肝损害占75.2%(85/113),肺损害占47.8%(54/113),血... 详细信息

关键词：恙虫病恙虫病东方体外斐反应

Impact of functional electrical stimulation on the early neuronal plasticity in the penumbra of rats with permanent focal cerebral ischemia

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Impact of functional electrical stimulation on the early neu...

新生儿感染性休克中全身炎性反应综合征新、旧诊断标准比较

跟踪先进康复治疗技术，提升与国际同步发展速度——中国康复医学会第七次全国康复治疗学术会议

作者： Dong-mei Jin~a,Zhi-qiang Zhuang~(aΔ),Tie-bin Yan~(a*),Yuan Peng~b,Yun Xiang~a,Yan-nan Fang~c and Raymond KY Tong~d a Department of Rehabilitation Medicine,Sun Yat-sen Memorial Hospital,Sun Yat-sen University, guangzhou,510120,china b Department of Rehabilitation Medicine,First People's Hospital in guangzhou City,guangzhou,510000, china c Department of Neurology,First Affiliated Hospital,Sun Yat-sen University,guangzhou,510080,china Department of Health Technology and Informatics,The Hong Kong Polytechnic University,Hong Kong, china

Objectives:This study investigated the neural mechanisms of functional electrical stimulation(FES)on motor behaviors,synaptic ultrastructures and protein expression of synaptophysin in the penumbra of rats with cerebral infarction. Methods:Following surgery of middle cerebral artery occlusion(MCAO),SD rats were randomly allocated to FES,placebo or sham-operated *** evaluations were assessed at baseline and each time point described *** ultrastructures in the penumbra were observed under transmission electron microscope and protein expression levels of synaptophysin in the penumbra were also measured by Western blot. Results:Compared with the rats in placebo groups,rats in FES group exhibited a significantly improved functional performance in prehensile traction test at the time point of 7d and 14 d(P<0.05),although the performance scores were still lower than that of rats in sham-operated groups at the corresponding time points. With prolonged treatment,the regressive ultrastructures of synapses in the penumbra of the infracted hemisphere of the rats in FES groups improved more markedly than that of placebo *** compared with the protein expression levels of synaptophysin in the penumbra of placebo group,the ones in FES group were up-regulated significantly at the time point of 3d,7d and 14d(P<0.05). Conclusion:Results from this study indicated that FES applied at the early stage of cerebral infarction could facilitate the functional recovery of the paralyzed limbs and activate the neuronal plasticity in the penumbra of the rats with cerebral ischemia.

关键词： Functional electrical stimulation Cerebral ischemia Penumbra Synaptophysin Plasticity

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实用儿科临床杂志 2008年第13期23卷 1017-1018,1035页

作者：陈晓文陈克正吕回陶莉广州市儿童医院新生儿科广州510120

目的探讨全身炎性反应综合征（SIRS）新、旧诊断标准对新生儿危重病的指导意义及其间的差异。方法以新生儿休克分度标准、新生儿SIRS新、旧诊断标准及新生儿脏器衰竭诊断标准为基础,对117例新生儿感染性休克进行临床分析。应用SPSS 13.... 详细信息

目的探讨全身炎性反应综合征（SIRS）新、旧诊断标准对新生儿危重病的指导意义及其间的差异。方法以新生儿休克分度标准、新生儿SIRS新、旧诊断标准及新生儿脏器衰竭诊断标准为基础,对117例新生儿感染性休克进行临床分析。应用SPSS 13.0软件进行统计学分析。结果感染性休克患儿117例中轻度休克20例,中度休克83例,重度休克14例。男90例,女27例。以出生3d内发病为多,多于入院3d内死亡,原发疾病以败血症为主。117例中,符合SIRS旧诊断标准者53例,符合率45.3%;符合SIRS新诊断标准者49例,符合率41.9%。在符合SIRS诊断标准的患儿中,符合2~4项诊断项目的例数及死亡数,新、旧标准间差异均无统计学意义,新、旧标准的符合率及病死率间的差异无统计学意义（Pa〉0.05）。无论新标准还是旧标准,SIRS诊断符合率最高者为体温改变,发生心率改变者较少。117例多伴2、3个脏器衰竭,以呼吸系统衰竭最常见,新、旧标准中符合相同脏器衰竭个数中的病死率的差异亦无统计学意义（P〉0.05）。结论从临床各方面比较,SIRS新、旧诊断标准对危重病判断的符合率均不高,说明SIRS新诊断标准并不比旧标准优越,提示需进一步完善新生儿SIRS诊断标准。

关键词：感染性休克全身炎性反应综合征诊断标准婴儿,新生

Aurora Kinase Small Molecule Inhibitor Destructs Mitotic Spindle,Suppresses Cell Growth,and Induces Apoptosis in Oral Squamous Cancer Cells

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Aurora Kinase Small Molecule Inhibitor Destructs Mitotic Spi...

第八次全国口腔颌面—头颈肿瘤会议

作者： Caobing Pan~1,Hongzhang Huang~1,Min Yan~2,Jine Yao~2,Jie Xu~2,Zijie Long2,Quentin Liu~2, (1.Department of Maxillofacial Surgery,Second Affiliated Hospital,Sun Yat-sen University,guangzhou 510120,china 2.State Key Laboratory of Oncology in South china,Cancer Center,Sun Yat-sen University,guangzhou 510060,china)

Mitotic Aurora kinases are required for accurate chromosome segregation during cell *** expression of Aurora-A (Aur-A) kinase results in centrosome amplification,aberrant spindles,and consequent *** the present study,we showed that Aurora kinase inhibitory small molecule VX-680 inhibited histone H3 phosphorylation at Ser10,a known in vivo substrate residue of Aurora kinase,in oral squamous cell carcinoma(OSCC) KB *** addition,monopolar spindle structures,typical abnormalities induced by inhibition of Aur-A,were generated in VX-680-treated *** of Aurora kinase led to reduced KB cell growth,as assessed by MTT *** blot analysis revealed that VX-680 caused cleavage of two critical apoptotic associated proteins, PARP and *** contrast,expression of cell survival factor Bcl-2 was reduced by VX-680 treatment in a dose-dependent ***,nuclear characteristic of DNA fragmentation,indicative of apoptotic cell death,was clearly observed in these OSCC cells with Aurora kinase inhibitory *** together,we showed that Aurora kinase inhibitory VX-680 led to apoptotic cell death in OSCC cells,suggesting a novel therapeutic target in oral cancer.

关键词： Aur-A,biomarker,molecular target,apoptosis The abbreviations used are:Aur-A,Aurora-A OSCC,Oral squamous cell carcinoma EGFR,Epidermal growth factor receptor BRCA1,Breast Cancer 1 TPX2,Target protein for xklp2 TACC,Transforming acidic coiled-coil Flt3,Fms-like tyrosine kinase 3 Abl Abelson tyrosine kinase

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对我国农村信用社生态位经营策略的思考

沈阳大学学报 2004年第5期16卷 26-28页

作者：李喜梅广东金融学院广东广州510120

借鉴"生态位"理论,认为我国农村信用社应将自身定位于服务农村,并要利用贴近农民的优势,为农村农民的经济发展提供有特色的金融产品和服务。

关键词：生态位农村信用社经营策略

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婴儿肺切除手术24例分析

新医学 2007年第11期38卷 718-720页

作者：杨盛春刘威夏园生梁建华广州市儿童医院胸外科 510120

目的:探讨婴儿肺切除手术的病因、手术方法、疗效和并发症。方法:分析24例婴儿肺切除手术患儿的临床资料。结果:24例中先天性肺囊肿10例、先天性大叶性肺气肿4例、先天性囊性腺瘤样畸形3例、肺隔离症1例,肺部感染合并脓胸6例。根据患儿... 详细信息

目的:探讨婴儿肺切除手术的病因、手术方法、疗效和并发症。方法:分析24例婴儿肺切除手术患儿的临床资料。结果:24例中先天性肺囊肿10例、先天性大叶性肺气肿4例、先天性囊性腺瘤样畸形3例、肺隔离症1例,肺部感染合并脓胸6例。根据患儿情况行患侧肺叶、肺楔型或肺段切除术,其中5例同时行脓胸纤维板剥离术。24例患儿术程均顺利。术后并发支气管胸膜瘘、气胸、肺出血各1例,经治疗后好转;1例先天性肺囊肿于术后3日死于肺功能不全;其余20例术后恢复良好。生存的23例于术后8～15日出院。20例获得随访2个月～11年,除1例先天性大叶性肺气肿出现轻度气促外,其他病例无明显异常。结论:婴儿肺切除手术主要的病因为先天性疾病与严重肺部感染,应根据患儿实际情况选择肺切除手术的方式,并重视治疗术后并发症,处理得当手术疗效颇佳。

关键词：肺切除手术婴儿肺畸形,先天性肺脓肿感染

Brain Tumour Surgery By Using Magnetoencephalography Localization

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Brain Tumour Surgery By Using Magnetoencephalography Localiz...