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Donor liver natural killer cells alleviate liver allograft acute rejection in rats

Hepatobiliary & Pancreatic Diseases International 2011年第4期10卷 386-392页

作者： Jian-Dong Yu,Tian-Zhu Long,Guo-Lin Li,Li-Hong Lv,Hao-Ming Lin,Yong-Heng Huang,Ya-Jin Chen and Yun-Le Wan Department of Hepatobiliary Surgery,Sun Yat-Sen Memorial Hospital,Sun Yat-Sen University,guangzhou 510120,china Department of Breast Surgery,guangzhou Women and Children’s Medical Center,guangzhou 510120,china [a] Department of Hepatobiliary Surgery Sun Yat-Sen Memorial Hospital Sun Yat-Sen University Guangzhou 510120 China [b] Department of Breast Surgery Guangzhou Women and Children's Medical Center Guangzhou 510120 China

BACKGROUND:Liver enriched natural killer (NK) cells are of high immune ***,the function of donor liver NK cells in allogeneic liver transplantation (LTx) remains ***:Ten Gy of whole body gamma-irradiation (WBI) from a 60 Co source at 0.6 Gy/min was used for depleting donorderived leukocytes,and transfusion of purified liver NK cells isolated from the same type rat as donor (donor type liver NK cells,dtl NKs) through portal vein was performed immediately after grafting the irradiated ***-transplant survival observation on recipients and histopathological detection of liver grafts were adoptive to evaluate the biological impact of donor liver NK cells on recipients’ survival in rat ***:Transfusion of dtl NKs did not shorten the survival time among the recipients of spontaneous tolerance model (BN to LEW rat) after rat LTx,but prolonged the liver graft survival among the recipients depleted of donor-derived leukocytes in the acute rejection model (LEW to BN rat).Compared to the recipients in the groups which received the graft depleted of donor-derived leukocytes,better survival and less damage in the allografts were also found among the recipients in the two different strain combinations of liver allograft due to transfusion of dtl ***:Donor liver NK cells alone do not exacerbate liver allograft acute ***,they can alleviate it,and improve the recipients’ survival.

关键词： liver transplantation natural killer cell donor transfusion graft rejection

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Promoter polymorphism of MRP1 associated with reduced survival in hepatocellular carcinoma

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World Journal of Gastroenterology 2010年第48期16卷 6104-6110页

作者： Jing Zhao,Bing-Yun Yu,Jin-E Yang,Key Laboratory of Gene Engineering of the Ministry of Education,School of Life Sciences,Sun Yat-sen (Zhongshan) University,guangzhou 510275,Guangdong Province,china Dao-Yuan Wang,Department of Medical Oncology,The First Affiliated Hospital of guangzhou Medical College,guangzhou 510120,Guangdong Province,china Key Laboratory of Gene Engineering of the Ministry of Education School of Life Sciences Sun Yat-sen (Zhongshan) University Xin Gang Xi Road 135# Guangzhou 510275 Guangdong Province China. lssyje@***

AIM:To investigate the effect of the G-1666A polymorphism in the multidrug resistance related protein-1 (MRP1) on outcome of hepatocellular carcinoma (HCC). METHODS:A cohort of 162 patients with surgically resected HCC who received no postsurgical treatment until relapse was studied. Genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism analysis. Electrophoretic mobility shift assay (EMSA) was used to evaluate the influence of the G-1666A polymorphism on the binding affinity of the MRP1 promoter with its putative transcription factors. RESULTS:Kaplan-Meier analysis showed that patients with GG homologues had a reduced 4-year disease-free survival compared with those carrying at least one A allele (P = 0.011). Multivariate Cox regression analysis indicated that the-1666GG genotype represented an independent predictor of poorer disease-free survival [hazard ratio (HR) = 3.067,95% confidence interval (CI):1.587-5.952,P = 0.001],and this trend became worse in men (HR = 3.154,95% CI:1.604-6.201,P = 0.001). A similar association was also observed between 4-year overall survival and the polymorphism in men (HR = 3.342,95% CI:1.474-7.576,P = 0.004). Moreover,EMSA suggested that the G allele had a stronger binding affinity to nuclear proteins. CONCLUSION:The MRP1-1666GG genotype predicted a worse outcome and was an independent predictor of poor survival in patients with HCC from Southeast china.

关键词： Multidrug resistance related protein-1 Single nucleotide polymorphism Hepatocellular carcinoma Prognosis

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Effect of hepatitis C virus core protein on modulation of cellular proliferation and apoptosis in hilar cholangiocarcinoma

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Hepatobiliary & Pancreatic Diseases International 2005年第1期4卷 71-74页

作者： Ru-Fu Chen, Zhi-Hua Li, Sheng-Quan Zou and Ji-Sheng Chen guangzhou, china Department of Hepatobiliary Surgery,Hospital of Zhongshan University, guangzhou 510120, china Department of Surgery,Tongji Hospital, Wuhan 430030, china Department of Hepatobiliary Surgery The Second Affiliated Hospital of Zhongshan University Guangzhou 510120 China. chenrf63@***

BACKGROUND: Hepatitis C virus (HCV) is believed to be an important human pathogen causing carcinoma. But the effect of HCV infection on the alteration of cellular pro- liferation and apoptosis and the relationship between the effect and the development of hilar cholangiocarcinoma are largely unknown. The aim of this study was to assess the effect of HCV core protein on proliferation and apoptosis of hilar cholangiocarcinoma. METHODS: HCV core protein (HCV C protein) was de- tected by peroxidase-antiperoxidase assay in surgical speci- mens from 48 patients with hilar cholangiocarcinoma. The apoptosis index ( AI) and PCNA index ( PI) in hilar cholangiocarcinoma were detected by in situ end labeling assay and streptavidin-biotin assay respectively. RESULTS: The expression of HCV C protein was observed in 32 (67.7%) of the 48 specimens of hilar cholangiocarci- noma. The mean ± standard deviation for AI and PI was 3.52%±0.64% and 46.24%±11.46% respectively. The AI of hilar cholangiocarcinoma specimens with HCV C protein expression was significantly lower than that of HCV C pro- tein negative specimens (P<0.01), whereas the PI of HCV C protein positive specimens was significantly higher than that of HCV C protein negative specimens (P<0.01). CONCLUSION: HCV C protein may promote the cellular proliferation of hilar cholangiocarcinoma and inhibit its cel- lular apoptosis.

关键词： hilar cholangiocarcinoma hepatitis C virus core protein apoptosis proliferation

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Efficacy of intramuscular BCG polysaccharide nucleotide on mild to moderate bronchial asthma accompanied with allergic rhinitis: a randomized, double blind, placebo-controlled study

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中华医学杂志(英文版) 2005年第19期000卷 13-21页

作者： LI Jing, LUO Ding-fen, LI Sui-ying, SUN Bao-qing and ZHONG Nan-shan guangzhou Institute of Respiratory Disease, First Affiliated Hospital of guangzhou Medical College, guangzhou 510120, china guangzhou Institute of Respiratory Disease First Affiliated Hospital of Guangzhou Medical College Guangzhou 510120 China

The extensive evidence of Th2 cytokine involvement in the pathogenesis of allergic diseases~ 1,2 suggests a potential target for immunomodulation. Because the Th1 and Th2 cells are reciprocally regulated, several recent studies on Th2-associated diseases including asthma have shown to reverse the Th2 response into a protective Th1 response.~ 3,4

关键词： asthma · allergic rhinitis · Bacillus Calmette-Guérin polysaccharide nucleotide

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Sequence Analysis and Structural Prediction of the Severe Acute Respiratory Syndrome Coronavirus nsp5

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Acta Biochimica et Biophysica Sinica 2005年第7期37卷 473-479页

作者： Jia-Hai LU Ding-Mei ZHANG Guo-Ling WANG Zhong-Min GUO Juan LI Bing-Yan TAN Li-Ping OU-YANG Wen-Hua LING Xin-Bing YU Nan-Shan ZHONG~1 The Public Health School of Sun Yat-Sen University,guangzhou 510080,china 1 guangzhou Institute of Respiratory Diseases,guangzhou Medical College,guangzhou 510120,china The Public Health School of Sun Yat-Sen University Guangzhou 510080 China

The non-structural proteins (nsp or replicase proteins) of coronaviruses are relativelyconserved and can be effective targets for *** studies have been conducted into the function of thesevere acute respiratory syndrome coronavirus (SARS-CoV) *** this study,bioinformatics methodswere employed to predict the secondary structure and construct 3-D models of the SARS-CoV GD *** and sequential comparison was performed to analyze the mutation trend of the polymerasensp5 gene during the epidemic process using a nucleotide-nucleotide basic local alignment search tool(BLASTN) and a protein-protein basic local alignment search tool (BLASTP).The results indicated that thensp5 gene was steady during the epidemic process and the protein was homologous with other coronavirusnsp5 *** protein encoded by the nsp5 gene was expressed in COS-7 cells and analyzed by sodiumdodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE).This study provided the foundation forfurther exploration of the protein's biological function,and contributed to the search for anti-SARS-CoVdrugs.

关键词： severe acute respiratory syndrome (SARS) severe acute respiratory syndrome coronavirus (SARS-CoV) non-structural protein (nsp) replicase secondary structure 3-D structure

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Experimental study of gene therapy with angiostatin gene in pancreatic cancer

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Hepatobiliary & Pancreatic Diseases International 2002年第3期1卷 452-457页

作者： Sheng-Li Nie Shi-Zhen Yuan the Department of Gastroenterology, Sun Yat-Sen Memorial Hospital, Medical College, Sun Yat-Sen University, guangzhou 510120, china Department of Gastroenterology Sun Yat-Sen Memorial Hospital Medical College Sun Yat-Sen University Guangzhou 510120 China. victorynie@***

Objective: To study the effects of angiostatin (AS) gene mediated by liposome on human pancreatic cancer cell line SW1990. Methods: Angiostatin gene was cloned into the eu- karyotic expression vector pRC/CMV. The recombi- nant of pRC/CMV-AS was introduced into the pan- creatic cancer cell line, SW1990. The mechanism of anti-tumor was studied and tested. Results: The eukaryotic expression vector pRC/ CMV-AS was identified by the restriction digest. pRC/CMV-AS was stably integrated into the target cells and expressed by Western blot and drug-sensi- tivity tests, and inhibited the vascular endothelial cells proliferation in vitro. In addition, the effects of the angiostatin vector on reducing the volume of tumors implanted in nude mouse models were also noted. Conclusion: This study demonstrated that the recom- binant pRC/CMV-AS mediated by liposome may play a potential role in the treatment of pancreatic cancer in the future.

关键词： angiostatin gene therapy pancreatic cancer vector angiogenesis liposome antiangiogenesis dormancy therapy

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深化少儿图书馆的读者工作

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图书馆论坛 2003年第4期23卷 121-123页

作者：李雯广州少年儿童图书馆广州510120

从延伸服务、读书活动和信息服务三方面 ,探讨如何完善少儿图书馆的读者服务工作 ,通过深化读者服务创造效益 ,以效益促进事业的发展。

关键词：少儿图书馆读者工作服务延伸读书活动信息服务效益

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Outcome assessment of stroke convalescence using multiple scales based on different dimensions

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Neural Regeneration Research 2006年第8期1卷 710-713页

BACKGROUND: Convalescence is an important stage of stroke treatment. A lot of patients have somatic and mental disorders at various degrees. The primary standard can only reflect partial conditions of somatic disorder; in addition, multiple dimensions of patients at the phase of stroke convalescence are further observed by using a lot of standards, such as signs and symptoms of traditional Chinese medicine, daily activity and psychological status. OBJECTIVE: To analyze the outcome assessments of the cases of stroke convalescence measured with different criteria consisting of various dimensions by a cross-sectional investigation of the condition of stroke convalescent patients. DESIGN: Scale evaluation. SETTING: Departments of Clinical Epidemiology Exploratory Development and Neurology, the Second Affiliated Hospital of guangzhou University of Traditional Chinese Medicine; National Center for Training of Design, Measurement and Evaluation in Clinical Research,guangzhou University of Traditional Chinese Medicine. PARTICIPANTS: A total of 194 stroke convalescent patients treated in the Second Affiliated Hospital of guangzhou University of Traditional Chinese Medicine from July 26, 2000 to February 28, 2001 were taken as subjects of the study. There were 126 males and 68 females aged from 40 to 89 years, and the illness course ranged from 14 to 181 days. All patients met diagnosis-treatment criteria of stroke (the second version)[DTCS(V2.0)] and various kinds of diagnostic points of cerebrovascular diseases; moreover, all patients provided confirmed consents. METHODS: They were assessed by assessment methods including the following assessment instruments: DTCS(V2.0), self-designed scale of traditional Chinese medicine (TCM) symptoms (28 symptoms and physical signs were scored as 0, 1, 2 marks from none to severity), modified Edinburgh-Scandinavia stroke scale (a total of 45 marks, 0 to 15 marks as mild defect, 16 to 30 as moderate defect, 31 to 45 as severe defect), mod

关键词： Outcome assessment of stroke convalescence using multiple scales based on different dimensions ADL

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网络环境下少儿图书馆读者服务工作的思考

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图书馆论坛 2003年第1期23卷 102-104页

作者：周丽冰广州少儿图书馆广东广州510120

文章分析了少儿图书馆读者服务工作中存在的问题。

关键词：少儿图书馆读者服务工作藏书建设服务创新网络化建设网络环境

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关于少儿图书馆数字化建设的思考

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图书馆论坛 2002年第4期22卷 55-56,60页

作者：钟燕玲广州少年儿童图书馆广东广州510120

在简要介绍国外、国内数字图书馆研究和建设方面的基础上。

关键词：数字图书馆少儿图书馆数字化建设

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