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Effects of Inoculation with Phosphate Solubilizing Bacteria on the Physiology,Biochemistry,and Expression of Genes Related to the Protective Enzyme System of Fritillaria taipaiensis ***

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Phyton-International Journal of Experimental Botany 2024年第2期93卷 247-260页

作者： Zhifen Shi Fumei Pan Xiaotian Kong Jiaqi Lang Mingyan Ye Qian Wu guangzhi wang Liang Han Nong Zhou College of Pharmacy Chengdu University of Traditional Chinese MedicineChengdu611130China College of Pharmacy Dali UniversityDali671000China College of Food and Biological Engineering Chongqing Three Gorges UniversityChongqing404120China Diqing Tibetan Autonomous Prefecture Institute of Plateau Biology Diqing674400China Chongqing Institute of Medicinal Plant Cultivation Chongqing408435China

Fritillaria taipaiensis *** is a widely used medicinal herb in treating pulmonary *** recent years,its wild resources have become scarce,and the demand for efficient artificial cultivation has significantly *** article is the first to apply phosphate solubilizing bacteria isolated from the rhizosphere soil of *** *** to the cultivation process of *** *** aim is to identify suitable reference strains for the artificial cultivation and industrial development of *** *** by examining the effects of various phosphate solubilizing bacteria and their combinations on photosynthesis,physiological and biochemical properties,and gene expression related to the protective enzyme system in *** *** experiment,conducted in pots at room temperature,included a control group(CK)and groups inoculated with inorganic phosphorussolubilizing bacteria:W1(Bacillus cereus),W2(Serratia plymuthica),W12(Bacillus cereus and Serratia plymuthica),and groups inoculated with organophosphorus-solubilizing bacteria:Y1(Bacillus cereus),Y2(Bacillus cereus),Y12(Bacillus cereus and Bacillus cereus),totaling seven *** to CK,most growth indices in the bacterial addition groups showed significant differences,with W12 achieving the highest values in all indices except the leaf area *** content of photosynthetic pigments,photosynthetic parameters,and osmoregulatory substances increased variably in each bacterial treatment group.W12 exhibited the highest content of chlorophyll a and soluble protein,while W1 had the highest free proline *** activities of peroxidase(POD),superoxide dismutase(SOD),and catalase(CAT)in all inoculated groups were higher than in CK,with significant changes in SOD and CAT *** malondialdehyde(MDA)content in all inoculated groups was lower than in CK,with Y12 being the lowest,at approximately 30%of *** expression corresponding to these three enzymes also increased var

关键词： Fritillaria taipaiensis P.Y.Li phosphate solubiliozing bacteria photosynthesis physiology and biochemistry protective enzymes

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电厂汽机热力系统运行的优化研究

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现代制造技术与装备 2024年第1期60卷 73-75页

作者：王广智国能吉林龙华热电股份有限公司白城热电厂白城137000

以电厂汽机热力系统为对象,深入分析确保系统高效运行的多个优化因素。结合理论与实践探讨汽轮机与发电机组的匹配改进,以提高能量转换效率。研究指出,对汽机热力系统进行细致的能效优化,包括机组能效、疏水系统以及轴封系统和辅助蒸汽... 详细信息

以电厂汽机热力系统为对象,深入分析确保系统高效运行的多个优化因素。结合理论与实践探讨汽轮机与发电机组的匹配改进,以提高能量转换效率。研究指出,对汽机热力系统进行细致的能效优化,包括机组能效、疏水系统以及轴封系统和辅助蒸汽系统,可以显著降低能耗并增强运行稳定性。在系统运行操作方面,特别强调了机组启动流程的优化及气泵启动策略的改进,提高了整个系统的响应速度和可靠性。

关键词：电厂汽机热力系统能效优化

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Chemical characteristics and source apportionment of atmospheric particles during heating period in Harbin, China

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Journal of Environmental Sciences 2014年第12期26卷 2475-2483页

作者： Likun Huang guangzhi wang School of Food Engineering Harbin University of Commerce State Key Laboratory of Urban Water Resource and Environment Harbin Institute of Technology

Atmospheric particles（total suspended particles（TSPs）; particulate matter（PM） with particle size below 10 μm, PM10; particulate matter with particle size below 2.5 μm, PM2.5）were collected and analyzed during heating and non-heating periods in Harbin. The sources of PM10 and PM2.5were identified by the chemical mass balance（CMB） receptor *** indicated that PM2.5/TSP was the most prevalent and PM2.5was the main component of PM210, while the presence of PM10–100was relatively weak. SO-4and NO-3concentrations were more significant than other ions during the heating period. As compared with the non-heating period, Mn, Ni, Pb, S, Si, Ti, Zn, As, Ba, Cd, Cr, Fe and K were relatively higher during the heating period. In particular, Mn, Ni, S, Si, Ti, Zn and As in PM2.5were obviously higher during the heating period. Organic carbon（OC） in the heating period was 2–5 times higher than in the non-heating period. Elemental carbon（EC） did not change much. OC/EC ratios were 8–11 during the heating period, which was much higher than in other Chinese cities（OC/EC： 4–6）. Results from the CMB indicated that 11 pollution sources were identified, of which traffic, coal combustion, secondary sulfate, secondary nitrate, and secondary organic carbon made the greatest contribution. Before the heating period, dust and petrochemical industry made a larger contribution. In the heating period, coal combustion and secondary sulfate were higher. After the heating period, dust and petrochemical industry were higher. Some hazardous components in PM2.5were higher than in PM10, because PM2.5has a higher ability to absorb toxic substances. Thus PM2.5pollution is more significant regarding human health effects in the heating period.

关键词： Atmospheric particles Chemical characteristics Heating period Chemical mass balance

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Chemical characteristics and source apportionment of PM_(10) during a brown haze episode in Harbin, China

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Particuology 2011年第1期9卷 32-38页

作者： Likun Huang Chung-Shin Yuan guangzhi wang Kun wang School of Food Engineering Harbin University of Commerce Harbin 150076 China School of Municipal and Environmental Engineering Harbin Institute of Technology Harbin 150090 China State Key Laboratory of Urban Water Resources and Environments Harbin Institute of Technolngy Harbin 150090 China Institute of Environmental Engineering National Sun Yat-sen University Taiwan 80424 China

This study investigates the correlation between PM10 and meteorological factors such as wind speed, atmospheric visibility, dew point, relative humidity, and ambient temperature during a brown haze episode. In order to identify the potential sources of PMlo during brown haze episode, respirable par- ticulate matter （PM10） was collected during both non-haze days and haze days and further analyzed for metallic elements, ionic species, and carbonaceous contents. Among them, ionic species contributed 45-64% to PM10, while metallic elements contributed 7-21% to PM10 which was smaller than the other chemical constituents. The average OC/EC ratio （42） in haze days was about three times of the average OC/EC ratio （14） in non-haze days. By using chemical mass balance （CMB） receptor model, the major sources were apportioned, including traffics, incinerators, coal combustion, steel industry, petrochemical industry, and secondary aerosols, etc. The contribution to PM10 concentration of each source was calcu- lated for all the samples collected. The results showed that coal combustion was the major source of PM10 in non-haze days and secondary aerosols were the major source in haze days, followed by petrochemical industry, incinerators, and traffics, while other sources had negligible effect.

关键词： PM 10 Chemical analysis Meteorological factors CMB receptor model Source apportionment

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NLRP3炎症小体在姜黄素减轻七氟烷麻醉大鼠术后认知功能障碍中的作用

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中华麻醉学杂志 2024年第2期44卷 172-175页

作者：张江玲杨亚利杜献慧张继兵王广治河南省人民医院麻醉与围术期医学科、郑州大学人民医院郑州450000

目的评价NOD样受体蛋白3(NLRP3)炎症小体在姜黄素减轻七氟烷麻醉大鼠术后认知功能障碍中的作用。方法 SPF级健康雄性SD大鼠40只,17~18月龄,体质量580~600 g,采用随机数字表法分为4组(n=10):对照组(C组)、术后认知功能障碍组(P组)、姜黄... 详细信息

目的评价NOD样受体蛋白3(NLRP3)炎症小体在姜黄素减轻七氟烷麻醉大鼠术后认知功能障碍中的作用。方法 SPF级健康雄性SD大鼠40只,17~18月龄,体质量580~600 g,采用随机数字表法分为4组(n=10):对照组(C组)、术后认知功能障碍组(P组)、姜黄素组(CU组)和姜黄素+NLRP3炎症小体激活剂组(CN组)。采用吸入1.5%七氟烷探查腹腔的方法制备大鼠术后认知功能障碍模型。CU组和CN组大鼠姜黄素混悬液300 mg/kg灌胃,CN组大鼠尼日菌素钠盐5 mg/kg同时灌胃,1次/d,连续6 d,C组和P组大鼠等量生理盐水灌胃。随后采用水迷宫实验测定大鼠穿越原平台位置次数和目标象限停留时间,HE染色法观察海马组织病理学结果,TUNEL染色法检测神经元凋亡情况,Western blot法检测NLRP3、Bcl-2和Bax表达。结果与C组比较,P组大鼠穿越原平台位置次数减少,目标象限停留时间缩短,神经元凋亡率升高,NLRP3和Bax表达上调,Bcl-2表达下调(P<0.05);与P组比较,CU组大鼠穿越原平台位置次数增加,目标象限停留时间延长,神经元凋亡率降低,NLRP3和Bax表达下调,Bcl-2表达上调(P<0.05);与CU组比较,CN组大鼠穿越原平台位置次数减少,目标象限停留时间缩短,神经元凋亡率升高,NLRP3和Bax表达上调,Bcl-2表达下调(P<0.05)。结论 NLRP3炎症小体参与了姜黄素减轻七氟烷麻醉大鼠术后认知功能障碍的过程。

关键词： NLR家族,热蛋白结构域包含蛋白3 姜黄素七氟醚认知障碍

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基于网络药理学与分子对接研究中药乐脉颗粒治疗冠心病心绞痛的活性物质及作用机制

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成都中医药大学学报 2024年第2期47卷 29-38页

作者：汪明君毛洁王伟陈璐王光志成都中医药大学药学院四川成都611137 四川大学华西药业有限公司四川成都610041

目的:基于网络药理学及分子对接技术探究乐脉颗粒治疗冠心病心绞痛的物质基础及作用机制。方法:通过TCMSP、ETCM等数据库及文献检索收集乐脉颗粒的化学成分信息;利用SwissTargetPrediction数据库收集活性成分靶点;疾病靶点通过GeneCards... 详细信息

目的:基于网络药理学及分子对接技术探究乐脉颗粒治疗冠心病心绞痛的物质基础及作用机制。方法:通过TCMSP、ETCM等数据库及文献检索收集乐脉颗粒的化学成分信息;利用SwissTargetPrediction数据库收集活性成分靶点;疾病靶点通过GeneCards、DisGeNET等数据库收集;使用Venny获得交集靶点并绘制韦恩图;运用DAVID数据库进行生物功能注释与富集分析;使用Cytoscape软件构建乐脉颗粒活性成分-冠心病心绞痛靶标网络并进行网络拓扑学分析;通过AutoDock进行分子对接验证。结果:乐脉颗粒7味中药共有176个化学成分及975个靶点,冠心病心绞痛共有234个靶点,疾病与成分共有靶点65个;网络药理学技术分析得到乐脉颗粒通过丹酚酸A、芍药内酯苷、丹参新醌D、亚油酸、拟丹参醛、槲皮素和芍药苷等主要活性成分,参与PI3K-Akt信号通路、HIF-1信号通路、TNF信号通路和钙信号通路等多种信号通路,作用于IL-6、MMP-9、TNF、ICAM-1等关键靶点来影响炎症反应、缺氧反应和血栓形成等病理过程。分子对接结果显示主要成分与核心靶点之间结合情况好。结论:乐脉颗粒治疗冠心病心绞痛具有多成分、多靶点、多通路的特点,其作用机制主要与降低炎症反应,抑制血小板聚集等有关。

关键词：乐脉颗粒冠心病心绞痛网络药理学分子对接作用机制

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急性缺血性卒中不同时间窗影像学评价及应用进展

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中国卒中杂志 2024年第09期19卷 1094-1101页

作者：吴春艳尹雅诗王广志岳奎涛山东第二医科大学附属医院神经内科山东第二医科大学附属医院医学影像中心山东第二医科大学医学影像学院

急性缺血性卒中(acute ischemic stroke,AIS)具有高致残率和高致死率特征,早期识别AIS和准确评估AIS的时间窗,对提高治疗效果具有重要意义。近年来,多模态影像技术指导的治疗时间窗评估在AIS的个体化精细管理中越来越被重视。明确AIS不... 详细信息

急性缺血性卒中(acute ischemic stroke,AIS)具有高致残率和高致死率特征,早期识别AIS和准确评估AIS的时间窗,对提高治疗效果具有重要意义。近年来,多模态影像技术指导的治疗时间窗评估在AIS的个体化精细管理中越来越被重视。明确AIS不同发病时间的影像特征,对早期识别AIS、评估AIS患者发病时间、判断病情动态变化以及指导再灌注治疗有重要意义。目前单一成像模式已经无法满足AIS精细化管理的临床需求,通过合理的多模态影像联合应用,能够更好地实现AIS的早期诊断、发病时间评估和预后评价。本文分别对AIS不同发病时间CT、MRI的影像学特征,多模态影像技术的应用价值和进展,以及机器学习和人工智能技术在AIS诊断和治疗中的潜在价值进行综述。

关键词：急性缺血性卒中影像技术多模态发病至成像时间人工智能

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乌头母根生物碱含量差异的比较转录组学研究

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植物生理学报 2024年第4期60卷 685-696页

作者：胡漪文书剑琴高静罗敏陈丽娟王光志成都中医药大学药学院成都611137

为研究转录组水平上不同种源乌头中主要生物碱含量差异形成的原因,本文选用来自四川北川(BM)、四川青川(QM)及陕西南郑(NM)的乌头母根进行无参转录组测序。结果获得128 015个unigene,平均长度为726 bp, GC%大于44.61%。分别有52 496 (41... 详细信息

为研究转录组水平上不同种源乌头中主要生物碱含量差异形成的原因,本文选用来自四川北川(BM)、四川青川(QM)及陕西南郑(NM)的乌头母根进行无参转录组测序。结果获得128 015个unigene,平均长度为726 bp, GC%大于44.61%。分别有52 496 (41.01%)、41 296 (32.26%)、43 561 (34.03%)、34 145(26.67%)个unigene在Nr、Swiss-Prot、KEGG和KOG数据库中得到注释。BM-vs-NM、BM-vs-QM、NMvs-QM三个比较组中分别筛选得到1 540、1 289、1 828个差异表达基因,这些差异表达基因主要富集在代谢途径、次生代谢物的生物合成、内质网中的蛋白质加工等通路中。进一步分析差异表达基因与主要生物碱含量差异的相关性,结果显示,与不同种源乌头母根中主要生物碱含量差异相关的差异表达基因主要富集在类黄酮生物合成、植物MAPK信号通路、异喹啉生物碱生物合成等代谢通路中。本研究初步筛选了编码13个关键酶的候选基因和17个转录因子,推测其与乌头二萜类生物碱含量相关。研究结果可为乌头的良种选育和分子育种提供参考。

关键词：乌头生物碱转录组差异表达基因

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Assessment of blood and oxygen delivery to flaps of rhesus using near infrared steady-state spectroscopy

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Chinese Science Bulletin 2002年第21期47卷 1797-1802页

作者： TIAN Fenghua DING Haishu CAI Zhigang wang guangzhi ZHAO Fuyun Department of Biomedical Engineering School of MedicineTsinghuaUniversityBeijing 100084China Hospital of Stomatology Peking UniversityBeijing 100081China

Near infrared spectroscopy has been considered as a potentially ideal noninvasive technique for the postoperative monitoring of plastic surgery. In this study, 6 flaps were raised on rhesus monkeys’ forearms. Blood and oxygen delivery to these flaps was monitored following vascular occlusions and inhalation of pure oxygen. Optical fibers were adopted in the probe of the oximeter so that the cutaneous detection could be performed in reflectance mode. Different and repeatable patterns of changes were measured following vascular occlusions on flaps. It is clear that the near infrared spectroscopy is capable of postoperatively monitoring vascular problems in flaps, especially the venous problem. Near infrared spectroscopy showed high sensitivity to detect the dynamic changes in flaps induced by inhalation of pure oxygen in this study. The experimental results indicated that it was potential to assess tissue viability utilizing the dynamic changes induced by some noninvasive stimulation (such as inhaling

关键词： near infrared spectroscopy plastic surgery blood oxy-genation flap.

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Ultrafine nano-copper derived from dopamine polymerization&synchronous adsorption achieve electrochemical purification of nitrate to ammonia in complex water environments

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Chinese Chemical Letters 2024年第8期35卷 383-390页

作者： Xue Zhao Mengshan Chen Dan wang Haoran Zhang guangzhi Hu Yingtang Zhou Faculty of Chemistry and Chemical Engineering Yunnan Normal UniversityKunming 650092China National Engineering Research Center for Marine Aquaculture Marine Science and Technology CollegeZhejiang Ocean UniversityZhoushan 316004China College of Chemistry Nankai University Nankai UniversityTianjing 300071China Institute for Ecological Research and Pollution Control of Plateau Lakes School of Ecology and Environmental ScienceYunnan UniversityKunming 650504China

Electrochemical-nitrate-reduction-reaction(eNitRR)synthesis of ammonia is an effective way to treat ni-trate wastewater and alleviate the pressure of the Haber-Bosch ammonia production *** to develop effective catalysts to electrochemically reduce nitrate to ammonia and purify sewage under com-plex environmental conditions is the focus of current ***,the dopamine polymerization pro-cess and the[(C_(12)H_(8)N_(2))_(2)Cu]^(2+)complex embedding process were run simultaneously in time and space,and ultrafine Cu nanoparticles(Cu/CN)were effectively loaded on nitrogen-doped carbon after heat *** Cu/CN as the catalyst,the ammonia yield rate and Faradaic efficiency of the electrochemical conversion of NO_(3)^(-)to NH_(3)are highly 8984.0μg h^(−1)mg cat.^(−1)and 95.6%,*** in the face of complex water environments,such as neutral media,acidic media,coexisting ions,and actual nitrate wastewater,nitrate wastewater can be effectively purified to form high value-added *** strat-egy of simultaneous embedding increases the exposure rate of Cu sites,and the support of CN is also beneficial to reduce the energy barrier of ^(∗)NO_(3)*** study rationally designed catalysts that are beneficial to eNitRR,and considered the situation faced by practical applications during the research stage,reducing the performance gap between laboratory exploration and industrial applications.

关键词： Electrochemical nitrate reduction reaction Synthetic ammonia Sewage treatment Nano copper Dopamine

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