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Spy chemistry enables stable protein immobilization on iron oxide nanoparticles with enhanced magnetic properties

Journal of Materials Science & Technology 2023年第30期161卷 161-169页

作者： Chu Shi Jingyi Sheng guangxiang si Yan Li Yan Shen Ning Gu Jiangsu Key Laboratory for Biomaterials and Devices School of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210009China School of Biomedical Engineering and Informatics Nanjing Medical UniversityNanjing 211166China Medical School Nanjing UniversityNanjing 210093China

Iron oxide nanoparticles(IONPs)modified with functional proteins hold great promise in the biomedical ***,conventional protein modification strategies,such as adsorption and covalent coupling,are either unstable or nonspecific,or may result in the changes of protein structure and ultimately the loss of protein *** of active proteins on small-sized IONPs with a particle size of less than 30 nm is especially difficult due to their high surface ***,we developed a universal modifica-tion method based on Spy chemistry for rapid and stable protein immobilization on small-sized IONPs,which only requires the presence of active groups on the surface of nanoparticles that can couple with *** short,the SpyCatcher peptides were first coated on the surface of IONPs by cross-linking with activated groups,and then the SpyTag peptide fused with a model protein(enhanced green fluo-rescent protein,EGFP)was engineered(SpyTag-EGFP)and directly coupled to SpyCatcher-modified IONPs by self-assembly,which is spontaneous and robust while avoiding the effect of chemical reactions on functional protein *** obtained EGFP-functionalized IONPs exhibited enhanced and stable green fluorescence and improved magnetic *** addition,the cell internalization efficiency of EGFP-functionalized IONPs was significantly increased as compared to unmodified IONPs,providing an ideal solution for efficient cell labeling and *** conclusion,here we report a rapid and easy strategy for EGFP immobilization on IONPs based on Spy chemistry,which could be further adapted to other functional proteins in the ***-modified IONPs and SpyTag-X(arbitrary functional fusion proteins)hold great potential to be applied as a versatile platform for protein immobilization on IONPs and enable its multifunctional application in the future.

关键词： Iron oxide nanoparticles Spy chemistry Protein immobilization Magnetic resonance imaging Cell labeling

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Iron oxide nanoparticles:A promising approach for diagnosis and treatment of cardiovascular diseases

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Nano Research 2023年第11期16卷 12453-12470页

作者： Xue Bao Yu Mao guangxiang si Lina Kang Biao Xu Ning Gu Cardiovascular Disease Research Center Nanjing Drum Tower HospitalAffiliated Hospital of Medical SchoolMedical SchoolNanjing UniversityNanjing 210093China State Key Laboratory of Bioelectronics Jiangsu Key Laboratory for Biomaterials and DevicesSchool of Biological Sciences and Medical EngineeringSoutheast UniversityNanjing 210096China

Despite advances in diagnostic and therapeutic technologies for cardiovascular diseases(CVDs),it remains a leading cause of mortality and morbidity *** underscores the urgency for innovative approaches aiming at early and precise detection and treatment of CVDs to reduce the disease *** oxide nanoparticles(IONPs),with their unique magnetism and bioproperties,have shown great potential in this *** this review,we will begin with a brief overview of the synthesis and properties of *** will then focus on the latest applications of IONPs in CVDs,including diagnosis and *** use of IONPs in the integration of diagnosis and treatment for CVDs is a promising field,and will be addressed in a separate *** translational potential and challenges of IONPs will also be *** conclusion,ongoing research and development of IONP-based strategies are highly likely to address current challenges effectively,and offer more personalized and efficient options for the diagnosis and treatment of CVDs.

关键词： atherosclerosis cardiovascular disease diagnosis iron oxide nanoparticles theranostic treatment

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Extracellular magnetic labeling of biomimetic hydrogel-induced human mesenchymal stem cell spheroids with ferumoxytol for MRI tracking

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Bioactive Materials 2023年第1期19卷 418-428页

作者： Sen Yan Ke Hu Miao Zhang Jingyi Sheng Xueqin Xu Shijia Tang Yan Li Sheng Yang guangxiang si Yu Mao Yi Zhang Feimin Zhang Ning Gu State Key Laboratory of Bioelectronics Jiangsu Key Laboratory for Biomaterials and DevicesSchool of Biological Science and Medical EngineeringSoutheast UniversityNanjing210009JiangsuChina Laboratory of Oral Regenerative Medicine Technology School of Biomedical Engineering and InformaticsDepartment of Biomedical EngineeringNanjing Medical UniversityNanjing211166JiangsuChina Jiangsu Key Laboratory of Oral Diseases Affiliated Hospital of StomatologyNanjing Medical UniversityNanjing210029JiangsuChina Department of Biomedical Engineering School of MedicineTsinghua UniversityBeijing100084China Department of Colorectal Surgery The First Affiliated Hospital of Nanjing Medical UniversityNanjing210000JiangsuChina

Labeling of mesenchymal stem cells(MSCs)with superparamagnetic iron oxide nanoparticles(SPIONs)has emerged as a potential method for magnetic resonance imaging(MRI)tracking of transplanted cells in tissue repair studies and clinical *** of MSCs using clinically approved SPIONs(ferumoxytol)requires the use of transfection reagents or magnetic field,which largely limits their clinical *** overcome this obstacle,we established a novel and highly effective method for magnetic labeling of MSC spheroids using *** conventional methods,ferumoxytol labeling was done in the formation of a mechanically tunable biomimetic hydrogel-induced MSC ***,the labeled MSC spheroids exhibited strong MRI T2 signals and good ***,the encapsulated ferumoxytol was localized in the extracellular matrix(ECM)of the spheroids instead of the cytoplasm,minimizing the cytotoxicity of ferumoxytol and maintaining the viability and stemness properties of biomimetic hydrogel-induced MSC *** demonstrates the potential of this method for post-transplantation MRI tracking in the clinic.

关键词： Superparamagnetic iron oxide nanoparticles MSC labeling Mechanically tunable biomimetic hydrogel Spheroids MRI tracking

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Unveiling the next generation of MRI contrast agents:current insights and perspectives on ferumoxytol-enhanced MRI

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National Science Review 2024年第5期11卷 385-404页

作者： guangxiang si Yue Du Peng Tang Gao Ma Zhaochen Jia Xiaoyue Zhou Dan Mu Yan Shen Yi Lu Yu Mao Chuan Chen Yan Li Ning Gu Jiangsu Key Laboratory for Biomaterials and Devices School of Biological Science and Medical EngineeringSoutheast University Key Laboratory for Bio-Electromagnetic Environment and Advanced Medical Theranostics School of Biomedical Engineering and InformaticsNanjing Medical University Department of Radiology the First Affiliated Hospital of Nanjing Medical University MR Collaboration Siemens Healthineers Ltd. Department of Radiology Affiliated Nanjing Drum Tower Hospital of Nanjing University Medical School School of Mathematical Sciences Capital Normal University Nanjing Key Laboratory for Cardiovascular Information and Health Engineering Medicine Institute of Clinical Medicine Nanjing Drum Tower HospitalMedical SchoolNanjing University

Contrast-enhanced magnetic resonance imaging(CE-MRI) is a pivotal tool for global disease diagnosis and management. since its clinical availability in 2009, the off-label use of ferumoxytol for ferumoxytol-enhanced MRI(FE-MRI) has significantly reshaped CE-MRI practices. Unlike MRI that is enhanced by gadolinium-based contrast agents, FE-MRI offers advantages such as reduced contrast agent dosage, extended imaging windows, no nephrotoxicity, higher MRI time efficiency and the capability for molecular imaging. As a leading superparamagnetic iron oxide contrast agent, ferumoxytol is heralded as the next generation of contrast agents. This review delineates the pivotal clinical applications and inherent technical superiority of FE-MRI, providing an avant-garde medical-engineering interdisciplinary lens, thus bridging the gap between clinical demands and engineering innovations. Concurrently, we spotlight the emerging imaging themes and new technical breakthroughs. Lastly, we share our own insights on the potential trajectory of FE-MRI, shedding light on its future within the medical imaging realm.

关键词： ferumoxytol contrast agent contrast-enhanced MRI ferumoxytol-enhanced MRI

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纳米磁热复温玻璃化低温保存大鼠肾脏的研究

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真空科学与技术学报 2021年第9期41卷 883-890页

作者：冯海靠张淼毛宇司广祥李艳顾宁东南大学生物科学与医学工程学院江苏省生物材料与器件重点实验室南京210009

纳米磁热复温有望解决器官玻璃化低温保存复温的难题。本文测试了氧化铁纳米颗粒在VS55低温保护剂溶液中的胶体稳定性及磁热性能。结果表明,纳米颗粒VS55溶液分散体系即使在玻璃化冷冻磁热复温后,仍在较长时间内保持胶体稳定,且磁热性... 详细信息

纳米磁热复温有望解决器官玻璃化低温保存复温的难题。本文测试了氧化铁纳米颗粒在VS55低温保护剂溶液中的胶体稳定性及磁热性能。结果表明,纳米颗粒VS55溶液分散体系即使在玻璃化冷冻磁热复温后,仍在较长时间内保持胶体稳定,且磁热性能随交变磁场电流、纳米颗粒浓度的增加而提升。磁共振成像结果显示通过灌注能够基本实现纳米颗粒在大鼠肾脏组织中的均匀分布与完全洗脱。肾脏总体结构在玻璃化冷冻磁热复温后保持完整,但磁共振成像显示其内部血管网络可能受到一定程度的损伤。研究结果说明,通过进一步提升磁场强度或纳米颗粒的磁热性能,优化灌注加载流程,有望实现更加快速均匀的复温,从而在不久的将来解决器官玻璃化保存的复温难题。

关键词：磁性纳米颗粒磁热复温玻璃化保存大鼠肾脏灌注

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Effect of BNNTs/matrix interface tailoring on toughness and fracture morphology of hierarchical siC_f/siC composites

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Journal of Advanced Ceramics 2019年第4期8卷 555-563页

作者： guangxiang ZHU Qian FENG Jinshan YANG Jianbao HU Hongda WANG Yudong XUE Qingliang SHAN Shaoming DONG State Key Laboratory of High Performance Ceramics and Superfine Microstructure Shanghai Institute of CeramicsChinese Academy of SciencesShanghai 200050China Structural Ceramics and Composites Engineering Research Center Shanghai Institute of CeramicsChinese Academy of SciencesShanghai 200050China University of Chinese Academy of Sciences Beijing 100049China Analysis and Testing Center Donghua UniversityShanghai 201600China

A thin BN interphase is applied on BNNTs surface to tailor the interfacial bonding between BNNTs and siC matrix in hierarchical siCf/siC composites. The thickness of BN interphase ranging from 10 to 70 nm can be optimized by chemical vapor deposition after BNNTs are in situ grown on siC fiber surface. Without BN interphase, the fracture toughness of hierarchical si Cf/si C composites can be impaired by 13.6% due to strong interfacial bonding. As long as BN interphase with 30–45 nm thickness is applied, the interfacial bonding can be optimized and fracture toughness of hierarchical composites can be improved by 27.3%. It implies that tailoring BNNTs/matrix interface by depositing a layer of BN interphase is in favor of activating energy dissipation mechanisms at nanoscale induced by BNNTs.

关键词： boron nitride nanotubes(BNNTs) interface nanocomposites toughness and toughening

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一种基于修饰技术的改进QC-LDPC码构造方法

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半导体光电 2016年第2期37卷 248-251页

作者：周光香袁建国高文春孙思重庆邮电大学光通信与网络重点实验室重庆400065

基于修饰技术提出了一种改进的准循环低密度奇偶校验(QC-LDPC)码的构造方法。该方法构造的QC-LDPC码具有较低的编码复杂度,其校验矩阵围长至少为6,避免了四环的出现,具有良好的围长特性。仿真分析表明:通过该构造方法构造的码率为93.7%... 详细信息

基于修饰技术提出了一种改进的准循环低密度奇偶校验(QC-LDPC)码的构造方法。该方法构造的QC-LDPC码具有较低的编码复杂度,其校验矩阵围长至少为6,避免了四环的出现,具有良好的围长特性。仿真分析表明:通过该构造方法构造的码率为93.7%的QC-LDPC(3969,3717)码在降低其编码复杂度的情况下,拥有与其对应的未应用修饰技术的QC-LDPC(3969,3719)码相媲美的纠错性能;并且在相同条件下,QC-LDPC(3969,3717)码的纠错性能要好于利用随机构造方法构造的PEG-LDPC(3969,3720)码,以及ITU-T G.975中已广泛用于光通信系统中的RS(255,239)码和LDPC(32640,30592)码,更适合于光通信系统。

关键词：准循环低密度奇偶校验码修饰技术净编码增益(NCG) 光通信

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MONTE-CARLO siMULATION USED FOR COMPOsiTION DETERMINATION OF THIN PSC FILM ON si SUBSTRATE

MONTE-CARLO SIMULATION USED FOR COMPOSITION DETERMINATION OF...

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The Third Chinese-Japanese Electron Microscopy Seminar

作者： JIN guangxiang DIN Zejun WU Ziqin Fundamental Physics Center,University of Science and Technology of China,Hefei,Anhui,China

Introduction Quantitative microanalysis of thin films on substrates is an important and challenging problem,especially in cases where an element is in common with the *** work has been done by Reuter with some success...

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周围神经损伤后背根神经节神经元合成抗氟化物酸性磷酸酶的变化及其保护作用

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中华实验外科杂志 1998年第3期15卷 267-268页

作者：刘红光洪光祥王发斌何善述同济医科大学附属协和医院手外科武汉430022 同济医科大学生化教研室

目的研究周围神经损伤后脊髓背根神经节神经元合成抗氟化物酸性磷酸酶(FRAP)的变化,并探索从大鼠胚胎肢芽中提取的活性物质(ELBE)对它的保护作用。方法随机切断si)大鼠的坐骨神经,建立一侧单盲端硅胶管囊模型,单纯损伤组内不加任何成分,... 详细信息

目的研究周围神经损伤后脊髓背根神经节神经元合成抗氟化物酸性磷酸酶(FRAP)的变化,并探索从大鼠胚胎肢芽中提取的活性物质(ELBE)对它的保护作用。方法随机切断si)大鼠的坐骨神经,建立一侧单盲端硅胶管囊模型,单纯损伤组内不加任何成分,ELBE保护组内注满ELBE,然后定量测量术后1天,4天,1周,2周,4周,6周背侧脊髓组织内FRAP的含量。结果 FRAP于损伤后4天开始下降,2周内下降最快,以后逐渐减慢;用ELBE保护后FRAP在损伤后1周才明显下降,且幅度较小,2周后即趋于平稳。结论 ELBE能提高神经损伤后神经节神经元的活性,减轻神经元损伤的程度,减少死亡的数量,因而有利于神经再生。

关键词：感觉神经元 FRAP 胚胎肢芽周围神经损伤

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改性HM沸石分子筛催化α-一异丙基萘异构化反应

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化学反应工程与工艺 2011年第4期27卷 338-342页

作者：陈倩文靳海波何广湘杨索和郭志武北京化工大学化学工程学院北京100029 北京石油化工学院化学工程学院北京102617

采用正硅酸乙酯化学液相沉积法对HM分子筛进行改性,并考察了正硅酸乙酯用量、反应温度和液时空速等对α-—异丙基萘异构化制备β-—异丙基萘反应的影响。结果表明,改性后的HM分子筛的骨架结构基本不变,但分子筛孔径减小,内表面酸性适当... 详细信息

采用正硅酸乙酯化学液相沉积法对HM分子筛进行改性,并考察了正硅酸乙酯用量、反应温度和液时空速等对α-—异丙基萘异构化制备β-—异丙基萘反应的影响。结果表明,改性后的HM分子筛的骨架结构基本不变,但分子筛孔径减小,内表面酸性适当降低。以正硅酸乙酯用量为0.6 mL/g改性得到的HM分子筛为催化剂,在反应温度为290℃,液时空速1.0mL/min和压力为1 0 MPa的条件下进行α-—异丙基萘异构化制β-—异丙基萘,α-—异丙基萘转化率为41.87%,β-—异丙基萘选择性为99 31%。采用van't Hoff方程计算得α-—异丙基萘异构化制备β-—异丙基萘的反应热为17.384 kJ/mol。

关键词：异构化异丙基萘化学液相沉积 HM沸石分子筛

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