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Journal of Integrative Agriculture 2024年第7期23卷 2178-2195页

作者： Peng Liu Langlang Ma Siyi Jian Yao He guangsheng yuan Fei Ge Zhong Chen Chaoying Zou Guangtang Pan Thomas Lübberstedt Yaou Shen State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China/Maize Research Institute Sichuan Agricultural UniversityChengdu 611130China Department of Agronomy Iowa State UniversityAmesIA 50011USA

Genetic transformation has been an effective technology for improving the agronomic traits of ***,it is highly reliant on the use of embryonic callus(EC)and shows a serious genotype *** this study,we performed genomic sequencing for 80 core maize germplasms and constructed a high-density genomic variation map using our newly developed pipeline(MQ2Gpipe).Based on the induction rate of EC(REC),these inbred lines were categorized into three *** low-REC germplasms displayed more abundant genetic diversity than the high-REC *** integrating a genome-wide selective signature screen and region-based association analysis,we revealed 95.23 Mb of selective regions and 43 REC-associated *** variants had phenotypic variance explained values ranging between 21.46 and 49.46%.In total,103 candidate genes were identified within the linkage disequilibrium regions of these REC-associated *** genes mainly participate in regulation of the cell cycle,regulation of cytokinesis,and other functions,among which MYB15 and EMB2745 were located within the previously reported QTL for EC *** leaf area-associated variants with large effects were closely linked to several REC-related loci,implying a potential synergistic selection of REC and leaf size during modern maize breeding.

关键词： maize genetic transformation embryonic callus selective signal association analysis

Genome assembly of the maize inbred line A188 provides a new reference genome for functional genomics

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The Crop Journal 2022年第1期10卷 47-55页

作者： Fei Ge Jingtao Qu Peng Liu Lang Pan Chaoying Zou guangsheng yuan Cong Yang Guangtang Pan Jianwei Huang Langlang Ma Yaou Shen Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region Maize Research InstituteSichuan Agricultural UniversityChengdu 611130SichuanChina Berry Genomics Corporation Beijing 100015China

The current assembled maize genomes cannot represent the broad genetic diversity of maize *** more genome sequences is critical for constructing a pan-genome and elucidating the linkage between genotype and phenotype in *** we describe the genome sequence and annotation of A188,a maize inbred line with high phenotypic variation relative to other lines,acquired by single-molecule sequencing and optical genome *** assembled a 2210-Mb genome with a scaffold N50 size of 11.61 million bases(Mb),compared to 9.73 Mb for B73 and 10.2 Mb for *** on the B73_Ref Gen_V4 genome,295 scaffolds(2084.35 Mb,94.30%of the final genome assembly)were anchored and oriented on ten *** analysis revealed that~30%of the predicted A188 genes showed large structural divergence from B73,Mo17,and W22 genomes,which causes high protein divergence and may lead to phenotypic variation among the four inbred *** a line with high embryonic callus(EC)induction capacity,A188 provides a convenient tool for elucidating the molecular mechanism underlying the formation of EC in *** our new A188 genome with previously reported QTL and RNA sequencing data revealed eight genes with large structural variation and two differentially expressed genes playing potential roles in maize EC induction.

关键词： Maize Embryonic callus A188 Genome assembly Single-molecule sequencing

文化多元与邂逅:电影作品中的华裔社区地方营造

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世界地理研究 2022年第6期31卷 1192-1203页

作者：林琳袁广盛王敏北京大学城市规划与设计学院深圳518055 华南师范大学地理科学学院广州510631

经过艺术加工的影视作品反映现实的同时也高于现实,不断重构、书写现实空间。本研究援引邂逅地理及地方感等理论,以5部华裔社区空间题材的电影作品为主要分析对象,以文本分析法为主要研究方法,探讨五部影片中关于华裔社区的选择再现及... 详细信息

经过艺术加工的影视作品反映现实的同时也高于现实,不断重构、书写现实空间。本研究援引邂逅地理及地方感等理论,以5部华裔社区空间题材的电影作品为主要分析对象,以文本分析法为主要研究方法,探讨五部影片中关于华裔社区的选择再现及其背后蕴涵的社会文化意义。研究发现:①影视文本首先从民俗仪式与风水文化两个空间文化符号出发,构建地方性;在此基础上,电影文本以景观符号为切入口再现华裔社区的物质空间,实现新的地方营造。②文化多元的背景下,华裔社区成为“我者”文化与“他者”文化邂逅的重要场所。③非表征理论通过强调人的“身体”与族裔社区的相互关系从而在地方协商和情感融入框架下探索新的邂逅。研究还进一步探讨了电影文本中族裔社区的文化多元性与地方治理的关系,为族裔社区在未来实现更好的地方治理提供参考与建议。

关键词：文化多元邂逅地方营造想象地理再现地方治理

Transcriptomic responses in resistant and susceptible maize infected with Fusarium graminearum

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The Crop Journal 2020年第1期8卷 153-163页

作者： guangsheng yuan Xiujing He Hui Li Kui Xiang Li Liu Chaoying Zou Haijian Lin Jiali Wu Zhiming Zhang Guangtang Pan Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region of Ministry of Agriculture Maize Research InstituteSichuan Agricultural University

Gibberella ear rot(GER)caused by Fusarium graminearum(teleomorph Gibberella zeae)is a common maize disease that not only severely reduces grain yield but also contaminates maize grain with *** investigated the molecular mechanism underlying the host defense responses against pathogen infection using comparative transcriptomic *** injected *** spore suspensions into plants of resistant(IBM-81)and a susceptible(IBM-85)maize inbred line after pollination and performed RNA-seq 48,72,and 96 h after *** 487 and 410 differentially expressed genes(DEGs)were induced in the resistant and susceptible lines across three time points,indicating that a stronger defense response was activated in the resistant than in the susceptible *** them,198 genes commonly induced in the two lines were subjected to pathway analysis,revealing that most of the DEGs were closely associated with defense and a wide range of metabolic *** associated with pathogenesis-related protein 1(PR1)and regulation of salicylic acid were significantly enriched during *** infection,suggesting that these DEGs play dominant roles in maize resistance to *** results provide a resource for future gene discovery and facilitate elucidation of the complex defense mechanisms involved in resistance to GER.

关键词： Gibberella ear rot Fusarium graminearum Molecular mechanism RNA-seq Differentially expressed genes

Study on the Flowering Habit of Ardisia mamillata Hance

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Agricultural Biotechnology 2012年第3期1卷 12-14,17页

作者： Juan DU guangsheng yuan Meng YE College of Landscape Architecture Sichuan Agricultural University Maize research institute Sichuan Agricultural University College of Forestry Sichuan Agricultural University

This study aimed to investigate the flowering habit of Ardisia mamillata Hance, to provide theoretical basis for the crossbreeding of A. mamillata. Re- suits show that A. mamillata is not entomophilous but anemophilous. The florescence lasts 40 d from the last 10 d of June to the first 10 d of August; the florescence of single flower is about 1 -3 d; there are two peaks of individual florescence; the gregarious flowering belongs to abundant centralized pattern, which is conducive to the reproductive success and provides convenience for the crossbreeding of A. mamiUata.

关键词： Ardisia mamillata Hance Flowering phenology Pollination medium

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微分散液滴的光纤检测研究

化工学报 2024年第1期75卷 211-220页

作者：温唯谷袁志宏王凯骆广生清华大学化学工程系北京100084 清华大学化学工程联合国家重点实验室北京100084

微分散是微化工技术的重要组成部分,传统利用显微摄像分析和统计微分散液滴的方法成本高,不易推广。提出了一种基于光纤传感的微分散液滴在线检测技术,该方法基于漫反射光纤、光纤传感器、数据采集卡和LabVIEW程序实现了微通道内液滴生... 详细信息

微分散是微化工技术的重要组成部分,传统利用显微摄像分析和统计微分散液滴的方法成本高,不易推广。提出了一种基于光纤传感的微分散液滴在线检测技术,该方法基于漫反射光纤、光纤传感器、数据采集卡和LabVIEW程序实现了微通道内液滴生成频率和长度的在线检测,满足0.21 mm以上直径液滴的检测,检测频率上限为500 Hz,当液滴通过光纤时间超过15 ms时,还可检测液滴长度。研究结果表明光纤直径和光纤传感器响应时间是影响该方法检测性能的主要因素,与相似平台对比,以较低的成本实现了较高检测性能。

关键词：微通道微分散光电检测光纤传感器液滴检测

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Identification and functional analysis of miRNAs in developing kernels of a viviparous mutant in maize

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The Crop Journal 2013年第2期1卷 115-126页

作者： Haiping Ding Jian Gao Mao Luo Hua Peng Haijian Lin guangsheng yuan Yaou Shen Maojun Zhao Guangtang Pan Zhiming Zhang Institute of Maize Research Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region Ministry of AgricultureSichuan Agricultural University Drug Discovery Research Center of Luzhou Medical College Luzhou Medical College Sichuan Tourism University College of Life and Basic Science Sichuan Agricultural University

Given the important roles of miRNAs in post-transcriptional gene regulation, identification of differentially expressed miRNAs will facilitate the elucidation of molecular mechanisms underlying kernel development. In this study, we constructed a small RNA library to comprehensively represent the full complement of individual small RNAs and to characterize miRNA expression profiles in pooled ears of maize(Zea mays L.) at 10, 15,20, 22, 25 and 30 days after pollination(DAP). At least 21 miRNAs were differentially expressed. The differential expression of three of these miRNAs, i.e., miR528a, miR167a and miR160b, at each stage was verified by qRT-PCR. The results indicated that these miRNAs might be involved in kernel development. In addition, the predicted functions of target genes indicated that most of the target genes are involved in signal transduction and cell communication pathways, particularly the auxin signaling pathway. The expression of candidate germination-associated miRNAs was analyzed by hybridization to a maize genome microarray, and revealed differential expression of genes involved in plant hormone signaling pathways. This finding suggests that phytohormones play a critical role in the development of maize kernels. We found that in combination with other miRNAs, miR528a regulated a putative laccase, a Ring-H2 zinc finger protein and a MADS box-like protein, whereas miR167a and miR160b regulated multiple target genes,including ARF(auxin response factor), a member of the B3 transcription factor family. All three miRNAs are important for ear germination, development and physiology. The small RNA transcriptomes and mRNA obtained in this study will help us gain a betterunderstanding of the expression and function of small RNAs in the development of maize kernel.

关键词： Ear germination-associated miRNAs Microarray hybridization qRT-PCR Zea mays

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浅析话语分析方法在地缘环境解析中的应用

热带地理 2021年第6期41卷 1132-1141页

作者：袁广盛安宁华南师范大学地理科学学院广州510631 华南师范大学亚洲地理研究中心广州510631

话语分析(DiscourseAnalysis)是一种在社会科学中广泛应用的研究方法。文章主要探讨了话语分析在地缘环境解析中的应用现状和前景。首先,对话语分析涉及的概念、理论和路径进行了辨析,展示了话语分析在人文地理学研究中的案例,重点关注... 详细信息

话语分析(DiscourseAnalysis)是一种在社会科学中广泛应用的研究方法。文章主要探讨了话语分析在地缘环境解析中的应用现状和前景。首先,对话语分析涉及的概念、理论和路径进行了辨析,展示了话语分析在人文地理学研究中的案例,重点关注了话语分析在地缘环境解析中的应用。其次,对比了地缘环境解析中的其他常用方法,加深对话语分析方法特点的了解,同时讨论了话语分析在未来地缘环境解析中运用的其他可能。最后,结合当下地理学研究方法不断创新的大背景,讨论了话语分析作为一种相对比较传统的研究方法在地缘政治学,或广泛一点,人文地理学中所扮演的角色和作用。

关键词：话语分析研究方法人文地理地缘政治地缘环境解析

机械通气联合血液灌流治疗重症有机磷中毒患者的效果探讨

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临床医学工程 2023年第3期30卷 289-290页

作者：岳豪杰袁广胜河南省临颍县人民医院急诊重症医学科河南漯河462600

目的探讨机械通气联合血液灌流治疗重症有机磷中毒患者的临床效果。方法选取2017年1月至2022年1月我院收治的80例重症有机磷中毒患者,随机分为对照组(n=40)和观察组(n=40)。两组患者均给予内科综合对症治疗,同时对照组采用机械通气治疗... 详细信息

目的探讨机械通气联合血液灌流治疗重症有机磷中毒患者的临床效果。方法选取2017年1月至2022年1月我院收治的80例重症有机磷中毒患者,随机分为对照组(n=40)和观察组(n=40)。两组患者均给予内科综合对症治疗,同时对照组采用机械通气治疗,观察组采用机械通气联合血液灌流治疗,比较两组的治疗效果。结果治疗后1 d、3 d、5 d,观察组的胆碱酯酶水平均高于对照组(P<0.05)。观察组的意识恢复时间、胆碱酯酶恢复正常时间及住院时间均短于对照组(P<0.05)。观察组的救治成功率高于对照组,死亡率低于对照组(P<0.05)。结论机械通气联合血液灌流治疗重症有机磷中毒效果显著,可有效促进患者预后恢复,降低患者死亡率,值得临床推广。

关键词：机械通气血液灌流重症有机磷中毒治疗效果

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聚乙烯微孔透气膜专用料的制备与性能研究

河南化工 2022年第8期39卷 27-31页

作者：袁广胜刘海育河南省核工业地质局河南核信恒达实业有限公司河南信阳464000

以聚乙烯共混体系为基础树脂,在聚烯烃弹性体(POE)为增韧剂与碳酸钙为致孔剂的作用下,研究了透气膜专用料的不同配比条件对聚乙烯透气膜加工、力学及透气等性能方面的影响,构筑了性能符合微孔透气膜要求的共混专用料。研究结果表明,采用... 详细信息

以聚乙烯共混体系为基础树脂,在聚烯烃弹性体(POE)为增韧剂与碳酸钙为致孔剂的作用下,研究了透气膜专用料的不同配比条件对聚乙烯透气膜加工、力学及透气等性能方面的影响,构筑了性能符合微孔透气膜要求的共混专用料。研究结果表明,采用POE,用量达到15%左右时,对共混体系的增益效果最佳;而采用碳酸钙作为致孔剂时,当其含量从30%增加到50%,透气膜的拉伸强度下降约10%,但孔隙率从27.3%增加到47.2%,透过性能大幅度提高。

关键词：聚乙烯微孔透气膜透气膜专用料聚烯烃弹性体碳酸钙透气膜性能