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Combination of AAV-delivered tumor suppressor PTEN with anti-PD-1 loaded depot gel for enhanced antitumor immunity

Acta Pharmaceutica Sinica B 2024年第1期14卷 350-364页

作者： Yongshun Zhang Lan Yang Yangsen Ou Rui Hu guangsheng Du Shuang Luo Fuhua Wu Hairui Wang Zhiqiang Xie Yu Zhang Chunting He cheng Ma Tao Gong Ling Zhang Zhirong Zhang Xun Sun Key Laboratory of Drug-Targeting and Drug Delivery System of the Education Ministry and Sichuan Province Sichuan Engineering Laboratory for Plant-Sourced Drug and Sichuan Research Center for Drug Precision Industrial TechnologyWest China School of PharmacySichuan UniversityChengdu 610041China Med-X Center for Materials College of Polymer Science and EngineeringSichuan UniversityChengdu 610065China

Recent clinical studies have shown that mutation of phosphatase and tensin homolog deleted on chromosome 10(PTEN)gene in cancer cells may be associated with immunosuppressive tumor microenvironment(TME)and poor response to immune checkpoint blockade(ICB)therapy.Therefore,efficiently restoring PTEN gene expression in cancer cells is critical to improving the responding rate to ICB therapy.Here,we screened an adeno-associated virus(AAV)capsid for efficient PTEN gene delivery into B16F10 tumor cells.We demonstrated that intratumorally injected AAV6-PTEN successfully restored the tumor cell PTEN gene expression and effectively inhibited tumor progression by inducing tumor cell immunogenic cell death(ICD)and increasing immune cell infiltration.Moreover,we developed an anti-PD-1 loaded phospholipid-based phase separation gel(PPSG),which formed an in situ depot and sustainably release anti-PD-1 drugs within 42 days in vivo.In order to effectively inhibit the recurrence of melanoma,we further applied a triple therapy based on AAV6-PTEN,PPSG^(@anti-PD-1)and CpG,and showed that this triple therapy strategy enhanced the synergistic antitumor immune effect and also induced robust immune memory,which completely rejected tumor recurrence.We anticipate that this triple therapy could be used as a new tumor combination therapy with stronger immune activation capacity and tumor inhibition efficacy.

关键词： Triple therapy Adeno-associated virus PTEN PPSG@anti-PD-1 CpG Immunogenic cell death Antitumor immune response Immune memory

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Innovator of in vitro virus culture— Dr. Chen-Hsiang Huang

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Protein & Cell 2011年第10期2卷 782-783页

作者： guangsheng cheng Institute of Microbiology Chinese Academy of SciencesBeijing 100101China

In 1954,American scientists John Franklin Enders,Thomas Huckle Weller and Frederick Chapman Robbins were awarded the Nobel Prize in Medicine and Physiology due to their contribution to the development of the technique to grow polio virus in cultures of human tissues.Their technique later became a powerful tool for the prevention of poliomyelitis in children.At the Nobel lecture on December 11,1954,when they talked about how they developed this technique,the three scientists mentioned that the methods had been used in the past by other workers,in particular Dr.Chen-Hsiang Huang(Fig.1).

关键词： culture prevention mentioned

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Functional genomics of Brassica napus:Progresses,challenges,and perspectives

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Journal of Integrative Plant Biology 2024年第3期66卷 484-509页

作者： Zengdong Tan Xu Han cheng Dai Shaoping Lu Hanzi He Xuan Yao Peng Chen Chao Yang Lun Zhao Qing-Yong Yang Jun Zou Jing Wen Dengfeng Hong Chao Liu Xianhong Ge Chuchuan Fan Bing Yi Chunyu Zhang Chaozhi Ma Kede Liu Jinxiong Shen Jinxing Tu guangsheng Yang Tingdong Fu Liang Guo Hu Zhao National Key Laboratory of Crop Genetic Improvement Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070China Yazhouwan National Laboratory Sanya 572025China

Brassica napus,commonly known as rapeseed or canola,is a major oil crop contributing over 13%to the stable supply of edible vegetable oil worldwide.Identification and understanding the gene functions in the B.napus genome is crucial for genomic breeding.A group of genes controlling agronomic traits have been successfully cloned through functional genomics studies in B.napus.In this review,we present an overview of the progress made in the functional genomics of B.napus,including the availability of germplasm resources,omics databases and cloned functional genes.Based on the current progress,we also highlight the main challenges and perspectives in this field.The advances in the functional genomics of B.napus contribute to a better understanding of the genetic basis underlying the complex agronomic traits in B.napus and will expedite the breeding of high quality,high resistance and high yield in B.napus varieties.

关键词： accelerate breeding Brassica napus functional genomics high resistance high yield

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A friend to man,"Dr.Feifan Tang:a story of causative agent of trachoma,from"Tang's virus"to Chlamydia trachomatis,to"Phylum Chlamydiae

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Protein & Cell 2011年第5期2卷 349-350页

作者： guangsheng cheng Ming Li George FGao Institute of Microbiology Chinese Academy of SciencesBeijing 100101China Beijing Institutes of Life Science Chinese Academy of SciencesBeijing 100101China

“Dr.Feifan Tang(F.F.Tang)was indeed‘a friend to man’as our old English eighteenth-century phrase has it;he loved the Chinese people and was a doughty fighter in the fundamental field of preventive medicine.With all friends here,I salute his memory and I am sure he will never be forgotten in China.”The famous British scientist Joseph Needham wrote this in 1979 in his letter to the then director of the National Vaccine&Serum Institute of China,when he received the obituary notice of Dr.F.F.Tang who had died in 1958.

关键词： has letter agent

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阻塞性睡眠呼吸暂停内型的量化技术

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中华结核和呼吸杂志 2024年第4期47卷 383-388页

作者：李广生彭程石瑜王彦陈宝元天津医科大学总医院呼吸与危重症医学科天津300052

阻塞性睡眠呼吸暂停(OSA)是睡眠中频繁发生呼吸暂停和(或)低通气,导致间歇低氧、高碳酸血症和睡眠结构紊乱,进而导致多系统损害。其病理生理学机制包括解剖结构异常、低觉醒阈值、高环路增益、肌肉反应性差等。由于OSA的潜在机制(即内型... 详细信息

阻塞性睡眠呼吸暂停(OSA)是睡眠中频繁发生呼吸暂停和(或)低通气,导致间歇低氧、高碳酸血症和睡眠结构紊乱,进而导致多系统损害。其病理生理学机制包括解剖结构异常、低觉醒阈值、高环路增益、肌肉反应性差等。由于OSA的潜在机制(即内型)存在个体差异,因而其治疗有效性及其预后也可能因取决于这些特征而不同。了解OSA的内型对于理解哪些患者最有可能通过非无创通气治疗消除OSA至关重要。量化内型是OSA精准治疗的核心,可为临床基于内型精准治疗OSA提供依据。

关键词：阻塞性睡眠呼吸暂停高碳酸血症多系统损害精准治疗无创通气治疗量化技术环路增益低通气

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多尺度行政区平衡视角下耕地空间布局优化

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农业工程学报 2024年第6期40卷 319-329页

作者：赵乐松陈莉珍肖瑶程迎轩聂嘉琦王红梅刘光盛华南农业大学公共管理学院广州510640 自然资源部华南热带亚热带自然资源监测重点实验室广州510700 广东省土地利用与整治重点实验室广州510640 广东省土地调查规划院广州510075

优化耕地空间布局对提升耕地生产能力、促进农业可持续发展具有重要意义。该研究以广东封开县为例,基于“现状耕地调出-可恢复地类调入”的微观调整逻辑,综合运用空间叠加、综合评价及蚁群优化算法,构建了耕地空间布局调整模型,以调入... 详细信息

优化耕地空间布局对提升耕地生产能力、促进农业可持续发展具有重要意义。该研究以广东封开县为例,基于“现状耕地调出-可恢复地类调入”的微观调整逻辑,综合运用空间叠加、综合评价及蚁群优化算法,构建了耕地空间布局调整模型,以调入调出数量平衡为约束条件实现了不同行政区尺度下的耕地布局优化。结果表明:(1)研究区共调出耕地1689.89 hm^(2),占耕地总面积的7.62%,整体上呈现出“全局零散、局部聚集”的分布特征。(2)研究区可恢复地类农业生产适宜性均值为67.04,具有开展作物种植的良好禀赋条件。(3)不同行政区尺度平衡情景下,调入结果在研究区北部空间差异显著,与镇内平衡情景相比,县内平衡情景下,调入耕地的农业生产适宜性均值提高了2.08,综合生态系统服务均值下降了0.67。(4)两种情景下,耕地的连片性、质量及稳定性均得到提升,因不受调入规模和行政单元空间范围限制,县内平衡情景下的提升效果更加明显,适当扩大可恢复地类筛选范围有利于提升整体耕地质量并降低重要生态用地筛选概率。该研究构建的耕地空间布局调整模型具有良好的适用性,可为制定农用地布局优化与整治提升方案提供参考依据。

关键词：土地利用耕地空间布局优化多尺度行政区县内平衡优化算法农业生产适宜性

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综合洗消体系对阻断非洲猪瘟病毒传入猪场的作用

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中国兽医杂志 2024年第2期60卷 152-156,F0003页

作者：张韵金睿妍何佳蔚程光胜丁红雷西南大学动物医学院动物支原体学实验室重庆北碚400715 重庆正大农牧食品有限公司重庆江北400021

切断非洲猪瘟病毒(ASFV)传播途径是防控非洲猪瘟的有效手段。本试验旨在通过在某种猪场建立综合洗消体系以阻断ASFV传入。对拟进入猪场的车辆在出发前和距猪场1 km处清洗和消毒,在猪场门口进行第3次消毒;人员进入猪场前洗澡和隔离,并将... 详细信息

切断非洲猪瘟病毒(ASFV)传播途径是防控非洲猪瘟的有效手段。本试验旨在通过在某种猪场建立综合洗消体系以阻断ASFV传入。对拟进入猪场的车辆在出发前和距猪场1 km处清洗和消毒,在猪场门口进行第3次消毒;人员进入猪场前洗澡和隔离,并将其衣物消毒;输入物资进场前消毒。通过实时荧光定量PCR检测经洗消的车辆、人员、物资和猪场内猪只携带ASFV核酸情况,酶联免疫吸附测定(ELISA)检测猪只的ASFV抗体水平。结果显示,2019、2020、2021和2022年,车辆ASFV检出率分别为2.6%、1.3%、0和0,人员ASFV检出率分别为3.0%、1.1%、0和0.9%,物资ASFV检出率分别为12.5%、0、5.9%和0;4年间,猪场内猪只的ASFV核酸和抗体检测均为阴性。结果表明,通过建立完善的洗消体系并开展ASFV检测,能有效阻断ASFV传入猪场。

关键词：非洲猪瘟病毒实时荧光定量PCR 酶联免疫吸附测定(ELISA) 车辆人员物资

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结合DCNN与短距条件随机场的遥感影像道路提取

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武汉大学学报（信息科学版） 2024年第3期49卷 333-342页

作者：赫晓慧陈明扬李盼乐田智慧周广胜郑州大学地球科学与技术学院河南郑州450052 郑州大学信息工程学院河南郑州450052 中国气象科学研究院郑州大学生态气象联合实验室河南郑州450052

深度卷积神经网络(deep convolutional neural network,DCNN)在高分辨率遥感图像自动道路提取领域被广泛应用,但现有方法难以对预测结果中像素间的上下文关系建模。针对此问题,已有研究利用全连接条件随机场(fully connected conditiona... 详细信息

深度卷积神经网络(deep convolutional neural network,DCNN)在高分辨率遥感图像自动道路提取领域被广泛应用,但现有方法难以对预测结果中像素间的上下文关系建模。针对此问题,已有研究利用全连接条件随机场(fully connected conditional random field,FullCRF)结合上下文信息对语义分割结果进行二次优化,但无法有效改善道路结构不连续问题。为改善道路结构的完整性,提出一种结合DCNN的短距条件随机场模型(short range conditional random field,SRCRF),SRCRF利用DCNN强大的特征提取能力并控制FullCRF的推理范围缓解过度平滑现象,解决道路提取结果中的结构不连续、不完整问题。实验结果表明,在Zimbawe-Roads数据集与cheng-Roads数据集中,SRCRF的F1分数相比DCNN分别上升约4.01%、3.73%,相比FullCRF分别上升约3.25%、2.28%。

关键词：遥感影像道路提取深度学习条件随机场语义分割

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MSCANet: multiscale context information aggregation network for Tibetan Plateau lake extraction from remote sensing images

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International Journal of Digital Earth 2023年第1期16卷 1-30页

作者： Zhihui Tian Xiaoyu Guo Xiaohui He Panle Li Xijjie cheng guangsheng Zhou School of Geoscience and Technology Zhengzhou UniversityZhengzhouPeople's Republic of China Ecometeorology Joint Laboratory of Zhengzhou University and Chinese Academy of Meteorological Science ZhengzhouPeople's Republic of China School of Information Engineering Zhengzhou UniversityZhengzhouPeople's Republic of China

Qinghai-Tibet Plateau lakes are important carriers of water resources in the‘Asian’s Water Tower’,and it is of great significance to grasp the spatial distribution of plateau lakes for the climate,ecological environment,and regional water cycle.However,the differences in spatial-spectral characteristics of various types of plateau lakes,and the complex background information of plateau both influence the extraction effect of lakes.Therefore,it is a great challenge to completely and effectively extract plateau lakes.In this study,we proposed a multiscale contextual information aggregation network,termed MSCANet,to automatically extract Plateau lake regions.It consists of three main components:a multiscale lake feature encoder,a feature decoder,and a Multicore Pyramid Pooling Module(MPPM).The multiscale lake feature encoder suppressed noise interference to capture multiscale spatial-spectral information from heterogeneous scenes.The MPPM module aggregated the contextual information of various lakes globally.We applied the MSCANet to the lake extraction of the Qinghai-Tibet Plateau based on Google data;additionally,comparative experiments showed that the MSCANet proposed had obvious improvement in lake detection accuracy and morphological integrity.Finally,we transferred the pre-trained optimal model to the Landsat-8 and Sentinel-2A dataset to verify the generalization of the MSCANet.

关键词： Remote sensing imagery The Qinghai-Tibet Plateau lake extraction deep learning multiscale feature context information aggregation

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Unveiling the planar deformation mechanisms for improved formability in pre-twinned AZ31 Mg alloy sheet at warm temperature

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Journal of Magnesium and Alloys 2023年第12期11卷 4659-4678页

作者： Xiaohuan Pan Lifei Wang Pengbin Lu Hua Zhang guangsheng Huang Liuwei Zheng Bin Xing Weili cheng Hongxia Wang Wei Liang Kwang Seon Shin Shanxi Key Laboratory of Advanced Magnesium-Based Materials College of Materials Science and EngineeringTaiyuan University of TechnologyTaiyuan 030024China National Engineering Laboratory for Industrial Big-data Application Technology Chongqing Innovation Center of Industrial Big-Data Co.Ltd.Chongqing 400707China School of Materials Science and Engineering Shanghai Jiao Tong UniversityShanghai 200240China Institute for Advanced Studies in Precision Materials Yantai UniversityYantai 264005China National Engineering Research Center for Magnesium Alloys College of Materials Science&EngineeringChongqing UniversityChongqing 400044China Magnesium Technology Innovation Center School of Materials Science and EngineeringSeoul National University1 Gwanak-roGwanak-guSeoul 08826South Korea

To investigate the role of pre-twins in Mg alloy sheets during warm planar deformation, the stretch forming is conducted at 200 ℃. Results suggest the formability of the pre-twinned AZ31 Mg alloy sheet is enhanced to 11.30 mm. The mechanisms for the improved formability and the deformation behaviors during the planar stretch forming are systematically investigated based on the planar stress states. The Schmid factor for deformation mechanisms are calculated, the results reveal that planar stress states extremely affect the Schmid factor for {10-12}twinning. The detwinning is activated and the prismatic slip is enhanced in the pre-twinned sheet, especially under the planar extension stress state in the outer region. Consequently, the thickness-direction strain is accommodated better. The dynamic recrystallization(DRX) type is continuous DRX(CDRX) regardless of the planar stress state. However, the CDRX degree is greater under the planar extension stress state.Some twin lattices deviate from the perfect {10-12} twinning relation due to the planar compression stress state and the CDRX. The basal texture is weakened when the planar stress state tends to change the texture components.

关键词： AZ31 Mg alloy sheet Planar stretch forming Planar stress states Continuous dynamic recrystallization {10-12}tensile twinning

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