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PAK6低表达对乳腺癌细胞增殖、迁移的影响及机制

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山东医药 2020年第28期60卷 41-44页

作者：程文曾安贵王毅李攀潘广锐攀枝花学院附属医院·攀枝花市中西医结合医院四川攀枝花617067 西南医科大学附属医院

目的观察p21激活激酶6(PAK6)低表达对乳腺癌细胞增殖、迁移的影响,并探讨其作用机制。方法常规培养人正常乳腺上皮细胞(MCF-10A)及乳腺癌细胞系(MDA-MB-435S、MCF-7、MDA-MB-361),用qRT-PCR法检测各细胞中PAK6 mRNA表达,乳腺癌细胞中PAK... 详细信息

目的观察p21激活激酶6(PAK6)低表达对乳腺癌细胞增殖、迁移的影响,并探讨其作用机制。方法常规培养人正常乳腺上皮细胞(MCF-10A)及乳腺癌细胞系(MDA-MB-435S、MCF-7、MDA-MB-361),用qRT-PCR法检测各细胞中PAK6 mRNA表达,乳腺癌细胞中PAK6 mRNA相对表达量均高于MCF-10A,其中MCF-7中最高,将MCF-7作为实验细胞。将MCF-7随机分为si-NC组、si-PAK6组,分别用脂质体2000转染NC siRNA(对照小干扰RNA)和抑制PAK6表达的小干扰RNA(PAK6 siRNA)。转染24 h收集细胞,用qRT-PCR法检测细胞内PAK6 mRNA表达,CCK-8法检测细胞增殖能力,Transwell实验检测细胞转移能力,Western blotting法检测细胞内磷酸化PI3K(pPI3K)、磷酸化AKT(pAKT)蛋白表达。结果si-NC组、si-PAK6组PAK6 mRNA相对表达量分别为1.06±0.12、0.45±0.08。与si-NC组比较,si-PAK6组PAK6 mRNA相对表达量低(Psi-NC组、si-PAK6组细胞增殖率分别为(100.00±0.00)%、(47.37±7.41)%。与si-NC组比较,si-PAK6组细胞增殖率低(Psi-NC组、si-PAK6组穿膜细胞数分别为(89.63±8.37)、(28.58±5.21)个。与si-NC组比较,si-PAK6组穿膜细胞数少(Psi-NC组、si-PAK6组的相对表达量分别为1.42±0.20、0.75±0.11,pAKT蛋白相对表达量分别为1.59±0.18、0.81±0.14。与si-NC组比较,si-PAK6组pPI3K、pAKT蛋白相对表达量均低(P均<0.05)。结论抑制PAK6表达可阻止乳腺癌细胞增殖、迁移,其作用机制与抑制PI3K/AKT信号通路激活有关。

关键词：乳腺癌 p21激活激酶6 细胞增殖细胞迁移 PI3K/AKT信号通路

卷取温度对合金元素在氧化铁皮与基体间界面富集的影响

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热加工工艺 2022年第1期51卷 86-89页

作者：李研李铁军滕华湘王海全蒋光锐刘李斌首钢技术研究院薄板研究所北京100043 首钢总公司绿色可循环钢铁流程北京市重点实验室北京100043 首钢工学院北京100043

为了保证高强钢的力学性能,往往钢中添加有较多合金元素,其中Mn、si、Al最为常见。本文研究了高强钢热轧板低温和高温卷取后合金元素的氧化行为。结果表明,在热轧卷取过程中,Mn、Al等合金元素在基体与氧化铁皮之间优先与氧元素结合形成... 详细信息

为了保证高强钢的力学性能,往往钢中添加有较多合金元素,其中Mn、si、Al最为常见。本文研究了高强钢热轧板低温和高温卷取后合金元素的氧化行为。结果表明,在热轧卷取过程中,Mn、Al等合金元素在基体与氧化铁皮之间优先与氧元素结合形成富集层。Mn、Al元素在基体与氧化铁皮间呈现不同的分布状态,Al元素呈现集中分布趋势,而Mn元素的分布较为分散,并且呈现出向氧化铁皮内部扩散的趋势。卷取温度对si元素的氧化富集有较为明显的影响,卷取温度较低时,si元素在界面位置未形成明显的氧化富集,而卷取温度较高时,si元素在氧化铁皮与基体之间会形成明显富集。

关键词：高强钢热轧氧化铁皮卷取富集

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冷轧高强钢选择性氧化行为研究

热加工工艺 2023年第3期52卷 103-105页

作者：李研李铁军蒋光锐郑晓飞鲍成人刘李斌滕华湘首钢技术研究院薄板研究所北京100043 首钢总公司绿色可循环钢铁流程北京市重点实验室北京100043 首钢工学院北京100043 首钢京唐联合有限责任公司冷轧作业部河北唐山063200

冷轧高强钢含有较多合金元素,如Mn,si,Al。在退火炉还原性气氛中,合金元素较铁元素更容易发生氧化,在带钢表层或次表层形成氧化富集,合金元素的氧化物往往以氧化物颗粒形式分布于晶界附近。为了研究不同露点、不同退火炉加湿位置对带钢... 详细信息

冷轧高强钢含有较多合金元素,如Mn,si,Al。在退火炉还原性气氛中,合金元素较铁元素更容易发生氧化,在带钢表层或次表层形成氧化富集,合金元素的氧化物往往以氧化物颗粒形式分布于晶界附近。为了研究不同露点、不同退火炉加湿位置对带钢表面合金元素氧化行为的影响,采用热镀锌模拟器模拟产线退火炉各段工况。在低露点条件下,带钢表面易于形成Mn元素的氧化富集,并形成粗大的氧化物颗粒,易对带钢表面质量造成不利影响。通过在适当温度段进行露点控制,可有效改善合金元素在带钢表面的富集氧化行为,有效改善带钢表面质量。

关键词：高强钢选择性氧化退火露点

MiR-499靶向HMGB1保护缺氧复氧心肌细胞损伤的分子机制探讨

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中国循证心血管医学杂志 2019年第12期11卷 1456-1460页

作者：周慧良甘受益李宾冯光瑞宋巧巧王淼咸宁市中心医院湖北科技学院附属第一医院心血管内科咸宁437000 湖北科技学院内科教研室咸宁437000

目的研究miR-499对缺氧复氧心肌细胞H9c2损伤的影响,并探讨其作用机制。方法运用免疫印迹法(Western blot)检测缺氧复氧心肌细胞中高迁移率族蛋白1(HMGB1)、B细胞淋巴瘤/白血病-2(Bcl-2)、免抗人单克隆抗体(Bax)、caspase-3的蛋白表达;... 详细信息

目的研究miR-499对缺氧复氧心肌细胞H9c2损伤的影响,并探讨其作用机制。方法运用免疫印迹法(Western blot)检测缺氧复氧心肌细胞中高迁移率族蛋白1(HMGB1)、B细胞淋巴瘤/白血病-2(Bcl-2)、免抗人单克隆抗体(Bax)、caspase-3的蛋白表达;将H/R+miR-con组(转染miRcon)、H/R+miR-499组(转染miR-499 mimics)、miR-con组(转染miR-con)、miR-499组(转染miR-499 mimics)、anti-miR-con组(转染anti-miR-con)、anti-miR-499组(转染anti-miR-499)、H/R+si-con组(转染si-con)、H/R+si-HMGB1组(转染si-HMGB1)、H/R+miR-499+Ctrl组(miR-499 mimics和pcDNA3.1共转染)、H/R+miR-499+HMGB1组(miR-499 mimics和pcDNA3.1-HMGB1共转染),均以脂质体法转染至H9c2细胞;实时荧光定量聚合酶链锁反应(qRT-PCR)检测各组细胞中miR-499的表达;流式细胞术检测各组细胞的凋亡;双荧光素酶报告基因检测实验检测各组细胞的荧光活性。结果与NC组相比,H/R组细胞中HMGB1蛋白表达显著升高,miR-499表达显著降低(P<0.05);过表达miR-499或敲减HMGB1均可显著下调H/R H9c2细胞凋亡率,显著下调Bax、caspase-3的蛋白表达,上调Bcl-2的蛋白表达(P均<0.05);过表达HMGB1可逆转miR-499对H/R H9c2细胞凋亡的抑制作用。结论MiR-499可抑制缺氧复氧心肌细胞H9c2凋亡,保护心肌细胞,其机制与靶向HMGB1有关,将可为心脏病的治疗提供新靶点。

关键词： miR-499 高迁移率族蛋白1 缺氧复氧心肌细胞凋亡

CEPC Technical Design Report

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Radiation Detection Technology and Methods 2024年第1期8卷 I0003-I0016,1-1091页

作者： Waleed Abdallah Tiago Carlos Adorno de Freitas Konstantin Afanaciev Shakeel Ahmad Ijaz Ahmed Xiaocong Ai Abid Aleem Wolfgang Altmannshofer Fabio Alves Weiming An Rui An Daniele Paolo Anderle Stefan Antusch Yasuo Arai Andrej Arbuzov Abdesslam Arhrib Mustafa Ashry Sha Bai Yu Bai Yang Bai Vipul Bairathi Csaba Balazs Philip Bambade Yong Ban Tripamo Bandyopadhyay Shou-Shan Bao Desmond P.Barber Ayse Bat Varvara Batozskaya Subash Chandra Behera Alexander Belyaev Michele Bertucci Xiao-Jun Bi Yuanjie Bi Tianjian Bian Fabrizio Bianchi Thomas Biekotter Michela Biglietti Shalva Bilanishvili Deng Binglin Denis Bodrov Anton Bogomyagkov Serge Bondarenko Stewart Boogert Maarten Boonekamp Marcello Borri Angelo Bosotti Vincent Boudry Mohammed Boukidi Igor Boyko Ivanka Bozovic Giuseppe Bozzi Jean-Claude Brient Anastasiia Budzinskaya Masroor Bukhari Vladimir Bytev Giacomo Cacciapaglia Hua Cai Wenyong Cai Wujun Cai Yijian Cai Yizhou Cai Yuchen Cai Haiying Cai Huacheng Cai Lorenzo Calibbi Junsong Cang Guofu Cao Jianshe Cao Antoine Chance Xuejun Chang Yue Chang Zhe Chang Xinyuan Chang Wei Chao Auttakit Chatrabhuti Yimin Che Yuzhi Che Bin Chen Danping Chen Fuqing Chen Fusan Chen Gang Chen Guoming Chen Hua-Xing Chen Huirun Chen Jinhui Chen Ji-Yuan Chen Kai Chen Mali Chen Mingjun Chen Mingshui Chen Ning Chen Shanhong Chen Shanzhen Chen Shao-Long Chen Shaomin Chen Shiqiang Chen Tianlu Chen Wei Chen Xiang Chen Xiaoyu Chen Xin Chen Xun Chen Xurong Chen Ye Chen Ying Chen Yukai Chen Zelin Chen Zilin Chen Gang Chen Boping Chen Chunhui Chen Hok Chuen Cheng Huajie Cheng Shan Cheng Tongguang Cheng Yunlong Chi Pietro Chimenti Wen Han Chiu Guk Cho Ming-Chung Chu Xiaotong Chu Ziliang Chu Guglielmo Coloretti Andreas Crivellin Hanhua Cui Xiaohao Cui Zhaoyuan Cui Brunella D'Anzi Ling-Yun Dai Xinchen Dai Xuwen Dai Antonio De Maria Nicola De Filippis Christophe De La Taille Francesca De Mori Chiara De sio Elisa Del Core Shuangxue Deng Wei-Tian Deng Zhi Deng Ziyan Deng Bhupal Dev Tang Dewen Biagio Di Micco Ran Ding siqin Dingl Yadong Ding Haiyi Dong Jianin AGH University of Science and Technology Faculty of Physics and Applied Computer ScienceKrakow Anhui University HefeiAnhui Applied Physics Department Federal Urdu University of ArtsScience and TechnologyIslamabad Banaras Hindu University Institute of ScienceVaranasi Bandirma Onyedi Eylul University Bandirma Beihang University Beijing Beijing Computational Science Research Center Beijing Beijing Conveyi LTD Beijing Beijing Glass Research Institute Co. LtdBeijing Beijing Institute of Technology Beijing Beijing Normal University Beijing Brown University ProvidenceRI Budker Institute of Nuclear Physics Novosibirsk Cadi Ayyad University Laboratory of Fundamental and Applied PhysicsPolydisciplinary FacultyMarrakech Cairo University Giza Center for Theory and Computation National Tsing Hua UniversityHsinchu Central China Normal University WuhanHubei Central South University ChangshaHunan Centro de Fisica Teorica de Particulas Universidade Tecnica de LisboaLisboa Chengdu Kaitengsifang Digital Radio&TV Equipment Co. LtdChengduSichuan China academy of engineering physics MianyangSichuan China building materials academy Beijing China Center of Advanced Science and Technology Beijing China Institute of Atomic Energy Beijing China Jiliang University HangzhouZhejiang China Nuclear Instrument CO. LTDBeijing China University of Geosciences Beijing China University of Geosciences WuhanHubei China University of Mining and Technology XuzhouJiangsu China West Normal University NanchongSichuan Chongqing University Department of PhysicsChongqing Chulalongkorn University Bangkok City University of Hong Kong Department of PhysicsHong Kong Cockcroft Institute Daresbury Cornell University IthacaNY CPPM(AMU/IPhU&CNRS/INP) Marseille Dalian Minzu University DalianLiaoning Dalian University of Technology Department of PhysicsDalian Deutsches Elektronen-Synchrotron DESY Hamburg Dzhelepov Laboratory of Nuclear Problems of Joint Institute for Nuclear Research(DLNP JINR) Dubna East China University Of Science And Technol

The Circular Electron Positron Collider(CEPC)is a large scientific project initiated and hosted by China,fostered through extensive collaboration with international *** complex comprises four accelerators:a 30 GeV Linac,a 1.1 GeV Damping Ring,a Booster capable of achieving energies up to 180 GeV,and a Collider operating at varying energy modes(Z,W,H,and tt).The Linac and Damping Ring are situated on the surface,while the subterranean Booster and Collider are housed in a 100 km circumference underground tunnel,strategically accommodating future expansion with provisions for a potential Super Proton Proton Collider(SPPC).The CEPC primarily serves as a Higgs *** its baseline design with synchrotron radiation(SR)power of 30 MW per beam,it can achieve a luminosity of 5×10^(34)cm^(-2)s^(-1)per interaction point(IP),resulting in an integrated luminosity of 13 ab^(-1)for two IPs over a decade,producing 2.6 million Higgs *** the SR power to 50 MW per beam expands the CEPC's capability to generate 4.3 million Higgs bosons,facilitating precise measurements of Higgs coupling at sub-percent levels,exceeding the precision expected from the HL-LHC by an order of *** Technical Design Report(TDR)follows the Preliminary Conceptual Design Report(Pre-CDR,2015)and the Conceptual Design Report(CDR,2018),comprehensively detailing the machine's layout,performance metrics,physical design and analysis,technical systems design,R&D and prototyping efforts,and associated civil engineering ***,it includes a cost estimate and a preliminary construction timeline,establishing a framework for forthcoming engineering design phase and site selection *** is anticipated to begin around 2027-2028,pending government approval,with an estimated duration of 8 *** commencement of experiments and data collection could potentially be initiated in the mid-2030s.

关键词： initiated exceeding precise