检索结果-南通市图书馆

维普期刊数据库

Mechanism of high Li-ion conductivity in poly(vinylene carbonate)-poly(ethylene oxide)cross-linked network based electrolyte revealed by solid-state NMR

在线全文

学校读者我要写书评

暂无评论

Journal of Energy Chemistry 2024年第6期93卷 377-383,I0010页

作者： fan Li Tiantian Dong Yi Ji Lixin Liang Kuizhi Chen Huanrui Zhang Guanglei Cui guangjin Hou State Key Laboratory of Catalysis Dalian National Laboratory for Clean EnergyCollaborative Innovation Center of Chemistry for Energy MaterialsDalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023LiaoningChina University of Chinese Academy of Sciences Beijing 100049China Qingdao Industrial Energy Storage Research Institute Qingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesQingdao 266101ShandongChina

Solid polymer electrolytes(SPEs)have become increasingly important in advanced lithium-ion batteries(LIBs)due to their improved safety and mechanical properties compared to organic liquid ***-linked polymers have the potential to further improve the mechanical property without trading off Li-ion *** this study,focusing on a recently developed cross-linked SPE,i.e.,the one based on poly(vinylene carbonate)-poly(ethylene oxide)cross-linked network(PVCN),we used solid-state nuclear magnetic resonance(NMR)techniques to investigate the fundamental interaction between the chain segments and Li ions,as well as the lithium-ion *** utilizing homonuclear/heteronuclear correlation,CP(cross-polarization)kinetics,and spin-lattice relaxation experiments,etc.,we revealed the structural characteristics and their relations to lithium-ion *** is found that the network formation prevents poly(ethylene oxide)chains from crystallization,which could create sufficient space for segmental tumbling and Li-ion co nductio *** such,the mechanical property is greatly improved with even higher Li-ion mobilities compared to the poly(vinylene carbonate)or poly(ethylene oxide)based SPE analogues.

关键词： ssNMR Lithium-ion mobility Cross-link Solid polymer electrolyte

维普期刊数据库

A Phytophthora capsici RXLR Effector Targets and Inhibits a Plant,PPlase to Suppress Endoplasmic Reticulum-Mediated Immunity

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2018年第8期11卷 1067-1083页

作者： guangjin fan Yang Yang Tingting Li Wenqin Lu Yu DU Xiaoyu Qiang Qujiang Wen Weixing Shan State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection Northwest A&F University Yangling Shaanxi 712100 China State Key Laboratory of Crop Stress Biology for Add Areas and College of Agronomy Northwest A&F University Yangling Shaanxi 712100 China State Key Laboratory of Crop Stress Biology for Arid Areas and College of Horticulture Northwest A&F University Yangling Shaanxi 712100 China

Phytophthora pathogens secrete a large arsenal of effectors that manipulate host processes to create an environment conducive to pathogen colonization. However, the underlying mechanisms by which Phytophthora effectors manipulate host plant cells still remain largely unclear. In this study, we report that PcAvr3a12, a Phytophthera capsici RXLR effector and a member of the Avr3a effector family, suppresses plant immunity by targeting and inhibiting host plant peptidyl-prolyl cis-trans isomerase （PPlase）. Overexpression of PcAvr3a 12 in Arabidopsis thaliana enhanced plant susceptibility to P. capsici. FKBP15-2, an endoplasmic reticulum （ER）-Iocalized protein, was identified as a host target of PcAvr3a12 during early P. capsici infection. Analyses of A. thaliana T-DNA insertion mutant （fkbp15-2）, RNAi, and overexpression lines consistently showed that FKBP15-2 positively regulates plant immunity in response to Phytophthora infection. FKBP15-2 possesses PPlase activity essential for its contribution to immunity but is directly suppressed by PcAvr3a12. Interestingly, we found that FKBP15-2 is involved in ER stress sensing and is required for ER stress-mediated plant immunity. Taken together, these results suggest that P. capsici deploys an RXLR effector, PcAvr3a12, to facilitate infection by targeting and suppressing a novel ER-Iocalized PPlase, FKBP15-2, which is required for ER stress-mediated plant immunity.

关键词： RXLR effector Avr3a FKBP ER stress immunity Phytophthora capsici

Genome-wide analysis of NDR1/HIN1-like genes in pepper(Capsicum annuum L.)and functional characterization of CaNHL4 under biotic and abiotic stresses

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Horticulture Research 2020年第1期7卷 1611-1625页

作者： Changyun Liu Haoran Peng Xinyu Li Chaolong Liu Xing Lv Xuefeng Wei Aihong Zou Jian Zhang guangjin fan Guanhua Ma Lisong Ma Xianchao Sun Laboratory of plant immunity and ecological control of plant disease College of Plant ProtectionSouthwest University400716 ChongqingChina Department of Botany and Plant Biology Section of BiologyFaculty of ScienceUniversity of Geneva1211 Geneva 4Switzerland State Key Laboratory of North China Crop Improvement and Regulation College of Plant ProtectionHebei Agricultural University071001 BaodingChina

Plant NDR1/HIN1-like(NHL)genes play an important role in triggering plant defenses in response to biotic *** this study,we performed a genome-wide identification of the NHL genes in pepper(Capsicum annuum L.)and characterized the functional roles of these CaNHL genes in response to abiotic stresses and infection by different *** analysis revealed that CaNHLs can be classified into five distinct subgroups,with each group containing generic and specific *** element analysis showed that the majority of the promoter regions of the identified CaNHLs contain jasmonic acid(JA)-responsive and salicylic acid(SA)-responsive elements,and transcriptomic analysis revealed that CaNHL genes are expressed in all the examined tissues of *** CaNHL1,CaNHL4,CaNHL6,CaNHL10,CaNHL11,and CaNHL12 genes were significantly upregulated under abiotic stress as well as in response to different pathogens,such as TMV,Phytophthora capsici and Pseudomonas *** addition,we found that CaNHL4 localizes to the plasma ***4-silenced pepper plants display significantly increased susceptibility to TMV,Phytophthora capsici and Pseudomonas syringae,exhibiting reduced expression of JA-related and SA-related genes and reduced ROS ***,transient overexpression of CaNHL4 in pepper increases the expression of JArelated and SA-related genes,enhances the accumulation of ROS,and inhibits the infection of these three ***,for the first time,we identified the NHL genes in pepper and demonstrated that CaNHL4 is involved in the production of ROS and that it also regulates the expression of JA-related and SA-related genes in response to different pathogens,suggesting that members of the CaNHL family play an essential role in the disease resistance of pepper.

关键词： pepper analysis functional

行为分析方法及其在铁路运输安全管理中的应用研究

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国铁路 2008年第11期 40-43页

作者：范广进北京交通大学交通运输学院硕士研究生北京100044

目前我国铁路安全管理存在"重处罚轻激励"问题。通过实施行为安全管理,采用行为安全管理过程及ABC行为分析方法,做到强化安全行为、弱化不安全行为,逐步形成良好的铁路安全文化。通过案例分析,说明采用行为安全管理方法可以减少不安全行... 详细信息

目前我国铁路安全管理存在"重处罚轻激励"问题。通过实施行为安全管理,采用行为安全管理过程及ABC行为分析方法,做到强化安全行为、弱化不安全行为,逐步形成良好的铁路安全文化。通过案例分析,说明采用行为安全管理方法可以减少不安全行为,降低事故发生率;提出强化行为安全管理措施,促进铁路运输安全行为管理。

关键词：行为安全行为分析铁路运输安全管理

维普期刊数据库

露天矿山岩质边坡软弱夹层赋存状态影响边坡稳定性规律研究

在线全文

学校读者我要写书评

暂无评论

重庆大学学报 2024年第4期47卷 22-33页

作者：樊鸿志王光进蓝蓉刘明生昆明理工大学国土资源工程学院昆明650093 云南省矿产资源开发与固废资源利用国际技术转移中心昆明650093 昆明有色冶金设计研究院股份有限公司昆明650051

软弱夹层作为一种典型地质结构是影响整个边坡安全稳定性的关键因素。为了更好地分析软弱夹层的性质及对边坡稳定性的影响,针对某含软弱夹层矿山露天岩质边坡,运用正交对照法对边坡软弱夹层的埋深、倾角、数量及夹层间结构面间距等4种... 详细信息

软弱夹层作为一种典型地质结构是影响整个边坡安全稳定性的关键因素。为了更好地分析软弱夹层的性质及对边坡稳定性的影响,针对某含软弱夹层矿山露天岩质边坡,运用正交对照法对边坡软弱夹层的埋深、倾角、数量及夹层间结构面间距等4种夹层赋存状态,进行变量4×4正交模拟,揭示了不同赋存状态下边坡稳定性及变形规律。研究结果表明,1)边坡软弱夹层的赋存状态对边坡稳定性有显著影响,随软弱夹层埋藏深度的增加边坡安全系数随之增大,当埋深达到一定值后,边坡安全系数趋于稳定;2)随着软弱夹层角度的增大,破坏机制表现为层间错动、顺层蠕滑和沿软弱结构面的剪切滑移递进式变化;3)随软弱夹层层数增多,边坡整体性下降致使变形不均匀,边坡安全系数减小,水平方向位移增大;4)对比同等埋深结构面间距较小的软弱夹层边坡,结构面间距对边坡安全系数的影响较小。

关键词：露天边坡软弱夹层赋存状态安全稳定性边坡变形

维普期刊数据库博看期刊

本氏烟NbMBF1c的克隆、表达及在TMV侵染过程中的功能

在线全文

学校读者我要写书评

暂无评论

中国农业科学 2022年第18期55卷 3543-3555页

作者：温玉霞张坚王琴王靖裴悦宏田绍锐樊光进马小舟孙现超西南大学植物保护学院植物病害生物学重庆市重点实验室

【目的】烟草花叶病毒（tobacco mosaic virus,TMV）是危害茄科、十字花科、葫芦科等农作物的重要病毒，给农业生产造成了巨大损失。通过分子克隆获得本氏烟（Nicotiana benthamiana）多蛋白桥梁因子1c(multiprotein bridging factor 1c... 详细信息

【目的】烟草花叶病毒（tobacco mosaic virus,TMV）是危害茄科、十字花科、葫芦科等农作物的重要病毒，给农业生产造成了巨大损失。通过分子克隆获得本氏烟（Nicotiana benthamiana）多蛋白桥梁因子1c(multiprotein bridging factor 1c,MBF1c)，综合应用生物信息学、细胞生物学以及分子生物学手段，明确NbMBF1c的抗病毒功能和机制，为作物的抗病毒育种提供理论依据。【方法】根据Sol Genomics Network中报道的NbMBF1c序列全长，设计引物克隆NbMBF1c全长序列；使用GeneDoc及MEGA X对NbMBF1c蛋白和其他物种中的同源蛋白序列进行比对并构建系统进化树；利用生物信息学分析NbMBF1c的基因特征和蛋白结构；使用实时荧光定量PCR检测其组织表达及其在TMV侵染中的表达；利用烟草脆裂病毒（tobacco rattle virus,TRV）诱导的基因沉默（virus induced gene silencing,VIGS）技术沉默NbMBF1c后通过摩擦接种TMV-GFP，明确NbMBF1c对病毒侵染的影响；构建pART27-GFP-NbMBF1c和pART27-Myc-NbMBF1c瞬时过表达载体，在本氏烟叶片中表达融合蛋白GFP-NbMBF1c后在共聚焦显微镜下观察亚细胞定位，表达融合蛋白Myc-NbMBF1c后接种病毒观察TMV-GFP侵染情况；采用实时荧光定量PCR检测沉默NbMBF1c后相关激素基因的表达，分析NbMBF1c影响病毒侵染的机制。【结果】NbMBF1c全长441 bp，编码146个氨基酸，包含一个保守结构域HTH(helix-turn-helix)；系统发育分析表明，NbMBF1c与绒毛状烟草（Nicotiana tomentosiformis）MBF1c(XP＿009614458.1)亲缘关系最近；NbMBF1c的表达具有特异性，在根中的表达量最高，其次是茎、叶和花；NbMBF1c定位于细胞质和细胞核中；沉默NbMBF1c会显著影响本氏烟株高，出现矮化等症状，接种TMV-GFP第5天时，沉默处理相较于对照组植株新叶病毒含量更高；瞬时过表达Myc-NbMBF1c融合蛋白，在注射叶接种TMV-GFP后，病毒侵染减少，但NbMBF1c并不与TMV组分互作；沉默NbMBF1c后，脱落酸（abscisic acid,ABA）合成关键酶基因NCED3、脱落酸受体PYL1、ABA信号转导因子ABAI1和蛋白激酶SnRK2E的表达量显著上调；茉莉酸（jasmonic acid,JA）合成途径AOS1上调表达，茉莉酸信号通路上的受体COI1下调表达。【结论】本氏烟MBF1家族NbMBF1c的表达受TMV侵染诱导，且主要定位于细胞质和细胞核。NbMBF1c作为植物抗TMV的正调控因子抑制病毒在本氏烟上的侵染。NbMBF1c不通过与TMV组分互作来抑制病毒侵染，而是通过调控植物激素的产生和信号传导来抑制病毒侵染。

关键词：本氏烟 NbMBF1c 烟草花叶病毒基因表达脱落酸茉莉酸

本氏烟半胱氨酸蛋白酶基因家族特征及其在TMV侵染中的功能

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

中国农业科学 2022年第21期55卷 4196-4210页

作者：裴悦宏李凤巍刘维娜温玉霞朱鑫田绍锐樊光进马小舟孙现超西南大学植物保护学院植物病害生物学重庆市重点实验室

【目的】明确抗病毒化合物氯吲哚酰肼（chloroinconazide,CHI）诱导的本氏烟（Nicotiana benthamiana）半胱氨酸蛋白酶（cysteine proteinase,CP）在烟草花叶病毒（tobacco mosaic virus,TMV）侵染过程中的作用及其基因家族特征，为理... 详细信息

【目的】明确抗病毒化合物氯吲哚酰肼（chloroinconazide,CHI）诱导的本氏烟（Nicotiana benthamiana）半胱氨酸蛋白酶（cysteine proteinase,CP）在烟草花叶病毒（tobacco mosaic virus,TMV）侵染过程中的作用及其基因家族特征，为理解茄科作物抗病毒分子机制及化学调控提供理论依据。【方法】利用全基因组策略，从茄科作物基因组数据库Sol Genomics Network中检索获得本氏烟NbCP的全家族基因序列，利用生物信息学分析NbCP基因家族的进化关系、Motif基序及启动子顺式作用元件。根据生物信息分析结果，利用实时荧光定量PCR技术分析TMV侵染后NbCP基因的表达，以此筛选出潜在抗病蛋白。使用烟草脆裂病毒（tobacco rattle virus,TRV）介导的基因沉默技术和马铃薯X病毒（potato virus X,PVX）介导的基因过表达技术验证该关键蛋白对TMV-GFP侵染的影响，结合实时荧光定量PCR手段探索该关键蛋白的抗病毒机制。【结果】NbCP家族共有24个成员，根据其染色体定位命名为NbCP1—NbCP24。进化关系分析表明NbCP分成5个亚家族，其中Group V含有7个NbCP，而Group I仅含2个NbCP;Motif分析表明不同分支NbCP具有相似的motif分布；启动子顺式作用元件分析表明NbCP家族基因受光调控，并且多个NbCP家族基因启动子含有茉莉酸甲酯、水杨酸甲酯、脱落酸、赤霉素和生长素等激素响应元件。结合生物信息学分析，从上述各个亚家族中分别筛选1个关键潜在抗病相关NbCP基因（NbCP8、NbCP12、NbCP13、NbCP18、NbCP22），并利用实时荧光定量PCR技术分析上述基因在TMV侵染第5天的表达，发现NbCP8表达差异性最大，上升了2.6倍，NbCP8在叶中的表达量最高，其次是茎和根，在花中的表达量最低。TRV介导的NbCP8沉默能显著增加TMV-GFP的侵染，而PVX介导的NbCP8过表达能显著抑制TMV-GFP侵染，表明NbCP8作为植物正调控因子抑制病毒侵染。沉默NbCP8显著抑制了水杨酸信号途径相关基因PR1以及茉莉酸信号途径相关基因MYC2的表达，但对NPR1、COI1以及脱落酸信号途径相关基因ABA1和NCED1的表达无显著影响；而过表达NbCP8则显著提高了PR1以及ABA1的表达，但对NPR1、MYC2、COI1和NCED1的表达无显著影响。【结论】本氏烟NbCP参与了植物胁迫防御，其中NbCP8在抗TMV防御中起显著作用，其分子机制是通过诱导水杨酸信号途径参与抗病防御，研究结果可为茄科作物抗病毒的分子机制提供证据。

关键词：本氏烟全基因组半胱氨酸蛋白酶 NbCP8 烟草花叶病毒

Fe3O4 nanoparticles elicit athe defense response in Nicotiana benthamiana against TMV by activating salicylate-dependent signaling pathways

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Fe3O4 nanoparticles elicit athe defense response in Nicotian...

Antimicrobial mechanisms of g-C3N4 nanosheets against the oomycetes Phytophthora capsici:disrupting metabolism and membrane structures and inhibiting vegetative and reproductive growth

中国植物病理学会2021年学术年会

作者：蔡璘 LIU Changyun GAO Changdan FENG Hui fan guangjin WANG Daibin RAN Mao XU Chen XU Xiaohong 孙现超 College of Plant Protection Southwest University

Control of plant pathogens can be realized by improving plant systemic resistance or direct antimicrobial activity against the *** nanoparticles were employed as a new strategy to directly kill pathogens(bacteria and fungus) and to act as nanofertilizers to facilitate plant ***,the improving the capabilities of the plant defense by nanoparticles against viruses towards plants are poorly ***,we developed nanoparticles as new plant immune inducer against virus in agriculture and systematically investigated the plant resistance to attack by tobacco mosaic virus(TMV) triggered by treating plants with Fe O nanoparticles(Fe O NPs).Interestingly,the antiviral action of Fe3 O4 NPs is mainly due to the regulation of plant cell signaling pathways by the ***,by foliar spraying,Fe O NPs were able to enter the leaf cells,reaching inside or near chloroplasts and were transported and accumulated throughout the whole plant *** Fe O NPs increased the plant dry weight and fresh weight,activated plant antioxidants,and upregulated SA synthesis and the expression of the SA-responsive PR genes(PR1 and PR2),which finally enhanced the plant disease-resistance ability against ***,the TMV particles were partly aggregated and fractured after pretreatment with the NPs in vitro for 2 ***,there was no significant difference between the NPs and the control group in the ability of the virus to colonize in the plant at 7 dpi after inoculating the pretreated TMV mixtures onto tobacco *** results suggest that the difference in the two outcomes was attributed to foliar spraying of Fe O NPs before TMV infection,which induced plant *** contrast,TMV inactivation by NPs was insufficient to prevent viral replication and accumulation in the host ***,Fe O NPs,are a novel,promising,efficient and candidate resistance inducer for plants,which broadens the avenues for researching and applying NPs in the fight agai

关键词： Fe3O4NPs Disease-resistance ability TMV

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Antimicrobial mechanisms of g-C3N4 nanosheets against the oo...