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Origin of a Subgenome and Genome Evolution of Allotetraploid Cotton Species

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Molecular Plant 2020年第9期13卷 1238-1240页

作者： Peng He Yuzhou Zhang guanghui xiao College of Life Sciences Shaanxi Normal UniversityXi'an 710119China Institute of Science and Technology(IST)Austria 3400 KlosterneuburgAustria

Cotton(Gossypium spp.)is one of the most important economic crops in the world and also a major source of natural fiber,oil,and *** morphology of cotton species varies from trailing herbaceous perennials to trees<10 *** other important crops,modern cotton cultivars are polyploids and have gone through polyploidization,evolution,and *** cotton genus comprises approximately 45 diploid(2n=2x=26)and seven tetraploid species(2n=4x=52)(Guan et al.,2014).

关键词： crops trail cotton

Using Genome-Referenced Expressed Sequence Tag Assembly to Analyze the Origin and Expression Patterns of Gossypium hirsutum Transcripts

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Journal of Integrative Plant Biology 2013年第7期55卷 576-585页

作者： Xiang Jin Qin Li guanghui xiao Yu-Xian Zhu State Key Laboratory of Protein and Plant Gene Research College of Life SciencesPeking University National Center for Plant Gene Research(Beijing)

We assembled a total of 297,239 Gossypium hirsutum （Gh, a tetraploid cotton, AADD） expressed sequence tag （EST） sequences that were available in the National Center for Biotechnology Information database, with reference to the recently published G. raimondii （Gr, a diploid cotton, DD） genome, and obtained 49,125 UniGenes. The average lengths of the U niGenes were increased from 804 and 791 bp in two previous EST assemblies to 1,019 bp in the current analysis. The number of putative cotton UniGenes with lengths of 3 kb or more increased from 25 or 34 to 1,223. As a result, thousands of originally independent G. hirsutum ESTs were aligned to produce large contigs encoding transcripts with very long open reading frames, indicating that the G. raimondii genome sequence provided remarkable advantages to assemble the tetraploid cotton transcriptome. Significant different distribution patterns within several GO terms, including transcription factor activity, were observed between D- and A-derived assemblies. Tran- scriptome analysis showed that, in a tetraploid cotton cell, 29,547 UniGenes were possibly derived from the D subgenome while another 19,578 may come from the A subgenome. Finally, some of the in silico data were confirmed by reverse transcription polymerase chain reaction experiments to show the changes in transcript levels for several gene families known to play key role in cotton fiber development. We believe that our work provides a useful platform for functional and evolutionary genomic studies in cotton.

关键词： Cotton fiber deep sequencing expressed sequence tag assembly functional genomics Gossypium.

Cotton BLH1 and KNOX6 antagonistically modulate fiber elongation via regulation of linolenic acid biosynthesis

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Plant Communications 2024年第7期5卷 138-156页

作者： Tingting Jia Huiqin Wang Shiyan Cui Zihan Li Yongcui Shen Hongbin Li guanghui xiao College of Life Sciences Shihezi UniversityShihezi 832003China College of Life Sciences Shaanxi Normal UniversityXi’an 710062China Zhengzhou Research Base State Key Laboratory of Cotton BiologySchool of Agricultural SciencesZhengzhou UniversityZhengzhou 450001China Geosystems Research Institute Mississippi State UniversityStarkvilleMS 39762USA These authors contributed equally to this work

BEL1-LIKE HOMEODOMAIN(BLH)proteins are known to function in various plant developmental ***,the role of BLHs in regulating plant cell elongation is still ***,we identify a BLH gene,GhBLH1,that positively regulates fiber cell *** transcriptomic and biochemical analyses reveal that GhBLH1 enhances linolenic acid accumulation to promote cotton fiber cell elongation by activating the transcription of GhFAD7A-1 via binding of the POX domain of GhBLH1 to the TGGA cis-element in the GhFAD7A-1 *** of GhFAD7A-1 in cotton significantly reduces fiber length,whereas overexpression of GhFAD7A-1 results in longer *** K2 domain of GhKNOX6 directly interacts with the POX domain of GhBLH1 to form a functional heterodimer,which interferes with the transcriptional activation of GhFAD7A-1 via the POX domain of *** of GhKNOX6 leads to a significant reduction in cotton fiber length,whereas knockout of GhKNOX6 results in longer cotton *** examination of the hybrid progeny of GhBLH1 and GhKNOX6 transgenic cotton lines provides evidence that GhKNOX6 negatively regulates GhBLH1-mediated cotton fiber *** results show that the interplay between GhBLH1 and GhKNOX6 modulates regulation of linolenic acid synthesis and thus contributes to plant cell elongation.

关键词： cotton fiber BLH1 KNOX6 linolenic acid biosynthesis fiber cell elongation

Single-Nucleotide Resolution Mapping of the Gossypium raimondii Transcriptome Reveals a New Mechanism for Alternative Splicing of Introns

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Molecular Plant 2014年第5期7卷 829-840页

作者： Qin Li guanghui xiao Yu-Xian Zhu State Key Laboratory of Protein and Plant Gene Research College of Life Sciences Peking University Beijing 100871 China National Center for Plant Gene Research (Beijing) Beijing 100101 China

Alternative splicing （AS） is a vital genetic mechanism that enhances the diversity of eukaryotic transcriptomes. Here, we generated 8.3 Gb high-quality RNA-sequencing data from cotton （Gossypium raimondii） and performed a systematic, comparative analysis of AS events. We mapped 85% of the RNA-sequencing data onto the reference genome and identified 154 368 splice junctions with 16 437 as events in 10197 genes. I ntron retention constituted the majority （40%） of all AS events in G. raimondii. Comparison across 11 eukaryote species showed that intron retention is the most common AS type in higher plants. Although transposable elements （TEs） were found in only 2.9% of all G. raimondii introns, they are present in 43% of the retained introns, suggesting that TE-insertion may be an important mechanism for intron retention during AS. The majority of the TE insertions are concentrated 0-40 nt upstream of the 3＇-splice site, substantially altering the distribution of branch points from preferred positions and reducing the efficiency of intron splicing by decreasing RNA secondary structure flexibility. Our data suggest that TE-insertion-induced changes in branch point-site distribution are important for intron retention-type AS. Our findings may help explain the vast differences in intron-retention frequencies between vertebrates and higher plants.

关键词： cotton RNA-seq alternative splicing transposable element intron retention.

A genome-wide identification of the BLH gene family reveals BLH1 involved in cotton fiber development

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Journal of Cotton Research 2020年第3期3卷 199-209页

作者： LIU Cuixia LI Zhifang DOU Lingling YUAN Yi ZOU Changsong SHANG Haihong CUI Langjun xiao guanghui Key Laboratory of the Ministry of Education for Medicinal Plant Resources and Natural Pharmaceutical Chemistry National Engineering Laboratory for Resouce Development of Endangered Crude Drugs in the Northwest of ChinaCollege of Life SciencesShaanxi Normal UniversityXi’an 710119ShaanxiChina College of Biology Pharmacy and Food Engineering Shangluo UniversityShangluo 726000ShaanxiChina State Key Laboratory of Cotton Biology State Key Laboratory of Crop Stress Adaptation and ImprovementSchool of Life SciencesHenan UniversityKaifeng 475001HenanChina School of Chemistry and Chemical Engineering Xianyang Normal UniversityXianyang 712000ShaanxiChina Zhengzhou Research Base State Key Laboratory of Cotton BiologyZhengzhou UniversityZhengzhou 450001China

Background:Cotton is the world’s largest and most important source of renewable natural ***1-like homeodomain(BLH)genes are ubiquitous in plants and have been reported to contribute to plant ***,there is no comprehensive characterization of this gene family in *** this study,32,16,and 18 BLH genes were identified from the ***,***,and *** genome,*** addition,we also studied the phylogenetic relationships,chromosomal location,gene structure,and gene expression patterns of the BLH ***:The results indicated that these BLH proteins were divided into seven distinct groups by phylogenetic *** them,25 members were assigned to 15 ***,gene structure,chromosomal location,conserved motifs,and expression level of BLH genes were investigated in *** profiles analysis showed that four genes(GhBLH1_3,GhBLH1_4,GhBLH1_5,and GhBLH1_6)from BLH1 subfamily were highly expressed during the fiber cell elongation *** expression levels of these genes were significantly induced by gibberellic acid and brassinosteroid,but not *** application of gibberellic acid significantly enhanced GhBLH1_3,GhBLH1_4,and GhBLH1_5 *** levels of GhBLH1_3 and GhBLH1_4 genes were significantly increased under brassinosteroid ***:The BLH gene family plays a very important role in many biological processes during plant growth and *** study deepens our understanding of the role of the GhBLH1 gene involved in fiber development and will help us in breeding better cotton varieties in the future.

关键词： Cotton G.hirsutum BLH gene family Fiber development

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“厨房事件”与印度禁止嫁妆法

学术论坛 2010年第8期33卷 143-149页

作者：肖光辉上海政法学院法律系法学理论教研室

印度禁止嫁妆的法案从颁布到现在已近50年,但是,印度并没有有效地阻止妇女所谓"厨房事件"的频繁发生,各地大量报道的有关妇女被焚烧、被杀案件说明了这一问题的严重性。妇女因嫁妆而伤亡的案件不断发生与印度的文化传统、社会变迁密切相... 详细信息

印度禁止嫁妆的法案从颁布到现在已近50年,但是,印度并没有有效地阻止妇女所谓"厨房事件"的频繁发生,各地大量报道的有关妇女被焚烧、被杀案件说明了这一问题的严重性。妇女因嫁妆而伤亡的案件不断发生与印度的文化传统、社会变迁密切相关,这种矛盾的现象也说明印度彻底废除嫁妆问题还有很长的路要走。

关键词：印度嫁妆法律

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难降解化纤废水预处理工业化实验

工业水处理 2013年第10期33卷 67-69页

作者：肖光辉高峰石家庄炼化分公司河北石家庄050032 石油化工科学研究院北京100083

采用高级氧化与絮凝沉淀组合工艺处理难降解化纤废水,并在石家庄炼化分公司进行了工业化实验。工业化实验结果表明,经过氨肟化废水预处理装置处理后的废水可生化性得到极大改善,废水的B/C从接近于0提高到0.3以上。经过预处理的废水未对... 详细信息

采用高级氧化与絮凝沉淀组合工艺处理难降解化纤废水,并在石家庄炼化分公司进行了工业化实验。工业化实验结果表明,经过氨肟化废水预处理装置处理后的废水可生化性得到极大改善,废水的B/C从接近于0提高到0.3以上。经过预处理的废水未对后续的生化系统造成冲击。

关键词：化纤废水高级氧化絮凝沉淀工业化实验

Introduction of Bottle Gourd DNA into Watermelon by Soaking Embryo for Resistance to Wilt Disease

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Hunan Agricultural Science ＆ Technology Newsletter 2001年第3期2卷 15-18页

作者： xiao guanghui,Wu Dexi,Liu Jianxiong,and xiao Lanyi Langping High-Teccch Xiangyuan Fruit and Melon Seeds and Seedlings Company,Changsha 410125,China Longping High - Tech Xiangyuan Fruit and Melon Seeds and Seedlings Company

In order to utilize wilt disease resistance of bottle gourd,total DNA of bottle gourd was introduced into watermelon through the method of aoeking *** DNA-introduced variant were cultured in contaminated soil to elect the wilt disease resistance for more than 3 genera- tions.2 high-resistant and 2 middle-resistant watermelon materials were obtained.

关键词： watermelon total DNA of bottle gourd resistance to wilt disease introduction

Utilization and Inheritance of Watermelon Resistance to Fusarium oxysporum *** Introduced from Bottle Gourd

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Hunan Agricultural Science ＆ Technology Newsletter 2001年第4期2卷 13-15页

作者： xiao guanghui,Liu Jianxiong,xiao Lanyi,Wu Dexi,and Luo Herong Longping High -TeA Xiangyuan Fruit and Melon Seeds and Seedlings Company,Changsha 410125,China Longping High - Tech Xiangyuan Fruit and Melon Seeds and Seedlings Company Changsha 410125 China

Sugar Baby susceptible to Fusarium oxysporum *** was crossed with high resistant male parents D3-1 and D3-2,respectively.F1 hybrids showed high *** segregation ratios of resistance to susceptibility of F2 and of BC1 hybrid population from Sugar Baby tallied with 3:1 and 1:1,*** results indicated that the resistance to Fusarium wilt was a kind of dominant inheritance controlled by mono-gene or mono-segment ***,5 hybrid combinations with fine character were *** them,3 were high resistant to Fusarium wilt,and 2 were medium resistant.

关键词： bottle gourd Fusarium wilt breeding for resistance watermelon