检索结果-南通市图书馆

In vivo and in vitro development of Tibetan antelope(Pantholops hodgsonii)interspecific cloned embryos

Frontiers of Agricultural Science and Engineering 2014年第1期1卷 21-29页

作者： guanghua su Lei CHENG Yu GAO Kun LIU Zhuying WEI Chunling BAI Fengxia YIN Li GAO Guangpeng LI Shorgan BOU The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology Key Laboratory of Herbivore Reproductive Biotechnology and Breeding Ministry of AgricultureInner Mongolia UniversityHohhot 010021China

The Tibetan antelope is endemic to the Tibetan Plateau,China,and is now considered an endangered *** a possible rescue strategy,the development of embryos constructed by interspecies somatic cell nuclear transfer(iSCNT)was *** antelope fibroblast cells were transferred into enucleated bovine,ovine and caprine *** cloned embryos were then cultured in vitro or in the oviducts of intermediate *** than 0.5%of the reconstructed antelope-bovine embryos cultured in vitro developed to the blastocyst ***,when the cloned antelope-bovine embryos were transferred to caprine oviducts,about 1.6%of the embryos developed to the blastocyst *** contrast,only 0.7%of the antelope-ovine embryos developed to the morula stage and none developed to blastocysts in ovine *** treatment of donor cells and bovine oocytes with trichostatin A did not improve the embryo development even when cultured in the oviducts of ovine and *** the antelope-bovine embryos,constructed from oocytes treated with roscovitine or trichostatin A,were cultured in rabbit oviducts 2.3%and 14.3%developed to blastocysts,*** is concluded that although some success was achieved with the protocols used,interspecies cloning of Tibetan antelope presents difficulties still to be *** mechanisms resulting in the low embryo development need investigation and progress might require a deeper understanding of cellular reprogramming.

关键词： interspecific nuclear transfer bovine ovine caprine oviduct apoptosis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The ecological adaptability of cloned sheep to free-grazing in the Tengger Desert of Inner Mongolia,China

引用

Frontiers of Agricultural Science and Engineering 2014年第3期1卷 191-200页

作者： Xinxin LI Huijuan WANG guanghua su Zhuying WEI Chunling BAI Wuni-MENGHE Yanhui HOU Changqing YU Shorgan BOU Guangpeng LI The Key Laboratory of National Education Ministry for Mammalian Reproductive Biology and Biotechnology Key Laboratory of Herbivore Reproductive Biotechnology and Breeding of Ministry of AgricultureInner Mongolia UniversityHohhot 010070China The Inner Mongolia Rangeland Ecology Institute Alashan 750306China

Since the birth of the first cloned sheep,somatic cell nuclear transfer technology has been successfully used to clone a variety of *** livestock have no apparent health risks,and the quality and safety of the cloned animal products are similar to non-cloned *** social behavior and environmental adaptability of postnatal cloned animals,especially when used for grassland farm production purposes,is *** the present study,the cloned Dorper sheep equipped with GPS location devices were free-grazed in a harsh natural environment similar to conditions commonly experienced by Mongolian *** main findings of this research were as follows.(1)Under free-grazing conditions,the cloned sheep showed excellent climatic and ecological *** extreme temperature conditions ranging from–30 to 40°C,the cloned sheep maintained acceptable body condition and behaved as other sheep.(2)The cloned sheep quickly adapted from a herd feeding strategy to the harsh environment and quickly exhibited a grazing regimen as other free-grazing sheep.(3)The cloned sheep exhibited free-grazing patterns and social behavior as other sheep.(4)The cloned sheep in the harsh environment thrived and produced healthy ***,the cloned Dorper sheep exhibited excellent ecological adaptation,which is an important consideration for breeding meat sheep by *** Dorper sheep readily adapted to the free-grazing conditions on the Mongolian plateau grassland,which attests to their ability to withstand harsh environmental conditions.

关键词： somatic cell nuclear transfer free-grazing synchronization Dorper sheep cloned animal ecology

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

660MW二次再热汽轮机启动步序的逻辑参数优化算法

引用

冶金能源 2022年第6期41卷 53-56页

作者：苏广华国能蚌埠发电有限公司

针对660 MW二次再热汽轮机启动步序较多、时延较长、疲劳寿命损耗增加的问题,以启动过程中温度场和应力场数值模拟为基础,采用X准则、Z准则结合的方式,优化启动步序的逻辑参数。实验结果表明,该方法能够有效解决汽轮机运行过程中过热的... 详细信息

针对660 MW二次再热汽轮机启动步序较多、时延较长、疲劳寿命损耗增加的问题,以启动过程中温度场和应力场数值模拟为基础,采用X准则、Z准则结合的方式,优化启动步序的逻辑参数。实验结果表明,该方法能够有效解决汽轮机运行过程中过热的问题,并缩短了启动时间,提高了最大应力、平均升温率,降低了低压转子关键部位最大热机械应力。虽然疲劳寿命损耗率增加到2.8%,但仍在合理范围内。

关键词：汽轮机启动步序逻辑参数应力场疲劳分析

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

a-Si:H,a-SiC:H和a-SiN:H合金系统中g值的理论计算(英文)

引用

固体电子学研究与进展 1989年第4期 472-473页

作者：陈光华苏玉成于工兰州大学物理系电子材料研究所

The g-values of the ESR signals caused by dangling bonds in a-Si:H, a-SiC:H and a-SiN:H alloy systems have been calculated for a cluster by using the CNDO method, and compared with the observed g-values. The detailed formula for the calculation of g-values was derived from the formula of ***. It is found that the g-values for dangling bonds on Si atoms are affected by the surrounding C or N atoms in a-SiC:H or a-SiN: H alloy systems and the calculated results are in good accordance with the observed ones. Some usefull discussions have also been made.

关键词：合金系统 a-Si:H,a-SiC:H a-SiN:H

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

RNA干扰在动物研究中的应用

引用

生物技术通报 2006年第3期22卷 46-48页

作者：苏光华张元跃湖南农业大学动物科技学院长沙410128

RNA干扰(RNAi)是指具有同源性的双链RNA分子导入细胞后,使促进与之同源的mRNA发生特异性降解的现象。随着对RNAi分子机制研究深入,它将成为研究动物基因功能、蛋白质功能、基因治疗、基因药物的强有力的工具。

关键词： RNA干扰动物

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A study on the dynamic tie points ASI algorithm in the Arctic Ocean

引用

Acta Oceanologica Sinica 2015年第11期34卷 126-135页

作者： HAO guanghua su Jie Key Laboratory of Physical Oceanography of Ministry of Education Ocean University of China

Sea ice concentration is an important parameter for polar sea ice monitoring. Based on 89 GHz AMSR-E （Advanced Microwave Scanning Radiometer for Earth Observing System） data, a gridded high-resolution passive microwave sea ice concentration product can be obtained using the ASI （the Arctic Radiation And Turbulence Interaction Study （ARTIST） Sea Ice） retrieval algorithm. Instead of using fixed-point values, we developed ASi algorithm based on daily changed tie points, called as the dynamic tie point ASI algorithm in this study. Here the tie points are expressed as the brightness temperature polarization difference of open water and 100% sea ice. In 2010, the yearly-averaged tie points of open water and sea ice in Arctic are estimated to be 50.8 K and 7.8 K, respectively. It is confirmed that the sea ice concentrations retrieved by the dynamic tie point ASI algorithm can increase （decrease） the sea ice concentrations in low-value （high-value） areas. This improved the sea ice concentrations by present retrieval algorithm from microwave data to some extent. Comparing with the products using fixed tie points, the sea ice concentrations retrieved from AMSR-E data by using the dynamic tie point ASI algorithm are closer to those obtained from MODIS （Moderate-resolution Imaging Spectroradiometer） data. In 40 selected cloud-free sample regions, 95% of our results have smaller mean differences and 75% of our results have lower root mean square （RMS） differences compare with those by the fixed tie points.

关键词： dynamic tie points ASI algorithm sea ice concentration AMSR-E MODIS

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A study of multiyear ice concentration retrieval algorithms using AMSR-E data

引用

Acta Oceanologica Sinica 2015年第9期34卷 102-109页

作者： HAO guanghua su Jie Key Laboratory of Physical Oceanography Ocean University of China

In recent years, the rapid decline of Arctic sea ice area （SIA） and sea ice extent （SIE）, especially for the multiyear （MY） ice, has led to significant effect on climate change. The accurate retrieval of MY ice concentration retrieval is very important and challenging to understand the ongoing changes. Three MY ice concentration retrieval algorithms were systematically evaluated. A similar total ice concentration was yielded by these algorithms, while the retrieved MY sea ice concentrations differs from each other. The MY SIA derived from NASA TEAM algorithm is relatively stable. Other two algorithms created seasonal fluctuations of MY SIA, particularly in autumn and winter. In this paper, we proposed an ice concentration retrieval algorithm, which developed the NASA TEAM algorithm by adding to use AMSR-E 6.9 GHz brightness temperature data and sea ice concentration using 89.0 GHz data. Comparison with the reference MY SIA from reference MY ice, indicates that the mean difference and root mean square （rms） difference of MY SIA derived from the algorithm of this study are 0.65×10^6 km^2 and 0.69×10^6 km^2 during January to March, -0.06×10^6 km^2 and 0.14×10^6 km^2during September to December respectively. Comparison with MY SIE obtained from weekly ice age data provided by University of Colorado show that, the mean difference and rms difference are 0.69×10^6 km^2 and 0.84×10^6 km^2, respectively. The developed algorithm proposed in this study has smaller difference compared with the reference MY ice and MY SIE from ice age data than the Wang＇s, Lomax＇ and NASA TEAM algorithms.

关键词： multiyear ice concentration retrieval algorithms AMSR-E data

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

用于戴瑞奶绵羊精液冷冻的Tris稀释液优化研究

引用

黑龙江畜牧兽医 2022年第14期 41-47,136,137页

作者：窦英杰郑重乌日汉马莹张立果刘信苏广华苏小虎李光鹏张立内蒙古大学生命科学学院呼和浩特010020 乌兰察布市畜牧工作站内蒙古乌兰察布012000 蒙天然牧业科技发展有限公司内蒙古乌兰察布012200

为了解决戴瑞奶绵羊冷冻精液质量偏低的问题,试验采集3只健康的24月龄戴瑞种公羊精液,分别用含0,40,80,120,160μmol/L白藜芦醇(RES)抗氧化剂的Tris稀释液稀释后冷冻,通过测定和计算冷冻前和冷冻后精液的精子活力、质膜完整率、顶体完... 详细信息

为了解决戴瑞奶绵羊冷冻精液质量偏低的问题,试验采集3只健康的24月龄戴瑞种公羊精液,分别用含0,40,80,120,160μmol/L白藜芦醇(RES)抗氧化剂的Tris稀释液稀释后冷冻,通过测定和计算冷冻前和冷冻后精液的精子活力、质膜完整率、顶体完整率、精子畸形率和抗氧化性能[丙二醛(MDA)含量、谷胱甘肽过氧化物酶(GSH-Px)活性和总抗氧化能力(T-AOC)]来确定RES的最适添加浓度。结果表明:80μmol/L RES添加量的精子活力、质膜完整率、顶体完整率均最高,平均值分别为(61.36±3.45)%、(40.31±1.20)%、(54.08±2.27)%;其中精子活力、质膜完整率均极显著高于0,160μmol/L添加量(P0.05),极显著高于160μmol/L添加量(P0.05),极显著低于0,160μmol/L(P0.05),极显著低于0,40μmol/L添加量(P<0.01),显著低于160μmol/L添加量(P<0.05)。80μmol/L添加量精子T-AOC和GSH-Px活性最高,平均值分别为(770.21±4.54)μmol/L和(65.49±1.29)U/mg,均极显著高于0,40,160μmol/L添加量(P<0.01),显著高于120μmol/L添加量(P<0.05)。说明在Tris稀释液中添加80μmol/L RES更适用于奶绵羊精液冷冻保存。

关键词：戴瑞奶绵羊冻精稀释液 Tris 白藜芦醇精液品质抗氧化能力

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

傢具CAD真实感图形处理系统

引用

山东科学 1990年第3期3卷 51-55页

作者：龚斌李建苏光华邓博超山东大学山东省计算中心烟台木材工业公司

本文介绍了傢具CAD真实感图形处理中的一些方法。由于采用了改进的消隐算法、合理的光照模型和纹理模型、产生的图形真实感强,图形生成效率高,对提高傢具CAD系统的整体效益具有重要作用。

关键词：家具真实感图形 CAD

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

奶牛血液保存条件和DNA提取方法的优化

引用

中国奶牛 2007年第5期 7-9页

作者：苏光华张元跃肖兵南燕海峰邓缘湖南农业大学动物科技学院长沙410128 湖南省畜牧兽医研究所长沙410131

本文研究中国荷斯坦牛血液在不同温度、时间保存的条件下(4℃保存0d为A组;-70℃保存30d为B组;4℃保存7d为C组)对DNA提取效果的影响和对比试剂盒法(E)、试剂盒改良法(F)、酚-氯仿法(G)提取中国荷斯坦牛血液基因组DNA的效果。试验结果表... 详细信息

本文研究中国荷斯坦牛血液在不同温度、时间保存的条件下(4℃保存0d为A组;-70℃保存30d为B组;4℃保存7d为C组)对DNA提取效果的影响和对比试剂盒法(E)、试剂盒改良法(F)、酚-氯仿法(G)提取中国荷斯坦牛血液基因组DNA的效果。试验结果表明:A组与B组差异不显著(P>0.05);A组、B组与C组差异显著(P0.05);E、F与G差异显著(P<0.05)。综合各方面因素,试剂盒改良法最好。

关键词：提取DNA 血液基因组DNA 中国荷斯坦牛

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：