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基于生物信息学对骨关节炎铁超载关键基因的筛选与验证

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中国组织工程研究 2025年第9期29卷 1972-1980页

作者：邓柯淇李光第 GOSWAMI ASHUTOSH 刘星余何孝勇贵州医科大学贵州省贵阳市550004 贵州医科大学附属医院骨外科贵州省贵阳市550004

背景:铁超载是指体内铁积累过多的情况,可引起各种组织的病理改变。目前,对于骨关节炎中铁超载相关的分子机制和潜在基因靶点,仍有待进一步的研究和探索。目的:通过生物信息学手段分析骨关节炎铁超载关键基因,并利用动物实验加以验证,... 详细信息

背景:铁超载是指体内铁积累过多的情况,可引起各种组织的病理改变。目前,对于骨关节炎中铁超载相关的分子机制和潜在基因靶点,仍有待进一步的研究和探索。目的:通过生物信息学手段分析骨关节炎铁超载关键基因,并利用动物实验加以验证,以期为铁超载角度防治骨关节炎提供新的思路。方法:使用GEO数据库和GeneCards数据库筛选出与骨关节炎相关的基因和铁超载相关的基因。然后,取两者的交集,得到骨关节炎和铁超载共同相关的基因集合。采用GO和KEGG富集分析筛选这些基因的功能和通路。为了进一步研究这些基因之间的相互作用,构建PPI网络,并利用Cytoscape软件的5种计算方法来识别骨关节炎铁超载的关键基因(Hub基因)。将12只雄性SD大鼠分为骨关节炎组与对照组(正常大鼠),每组6只,骨关节炎组大鼠采用改良Hulth方法建立膝骨关节炎模型,并使用PCR检测Hub基因在两组大鼠膝关节组织中的表达情况。结果与结论:①共获得51个骨关节炎铁超载基因,GO富集分析表明其主要参与细胞因子受体结合、趋化因子受体结合、细胞因子活性、生长因子受体结合及寡糖结合等过程;②KEGG富集分析表明骨关节炎铁超载基因主要参与肿瘤坏死因子信号通路、脂质和动脉粥样硬化等信号通路;③构建蛋白质-蛋白质相互作用网络,进一步分析获得细胞间黏附分子1(intercellular cell adhesion molecule-1,ICAM-1)、肿瘤坏死因子配体超家族成员11(tumor necrosis factor superfamily member 11,TNFSF11)、骨髓瘤细胞癌基因(myelocytomatosis oncogene,MYC)、Janus激酶2(janus kinase 2,JAK2)及白细胞介素6,5个骨关节炎铁超载Hub基因,动物实验证实其在对照组与骨关节炎组大鼠膝关节组织中的表达有显著差异(P<0.05);④结果显示,ICAM-1,TNFSF11,MYC,JAK2及白细胞介素6可作为骨关节炎铁超载的关键基因,有望成为防治骨关节炎的新靶点。

关键词：骨关节炎铁超载关键基因差异基因生物信息学动物实验实验验证生物标志物

Effects of fencing on vegetation and soil restoration in a degraded alkaline grassland in northeast China

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Journal of Arid Land 2014年第4期6卷 478-487页

作者： Qiang li DaoWei ZHOU YingHua JIN Minling WANG YanTao SONG guangdi li Northeast Institute of Geography and Agroecology Chinese Academy of Sciences University of Chinese Academy of Sciences School of Geography Science Northeast Normal University Graham Centre for Agricultural Innovation New South Wales Department of Primary Industries and Charles Sturt University

In order to restore a degraded alkaline grassland, the local government implemented a large restoration project using fences in Changling county, Jilin province, China, in 2000. Grazing was excluded from the protected area, whereas the grazed area was continuously grazed at 8.5 dry sheep equivalent（DSE）/hm2. In the current research, soil and plant samples were taken from grazed and fenced areas to examine changes in vegetation and soil properties in 2005, 2006 and 2008. Results showed that vegetation characteristics and soil properties improved significantly in the fenced area compared with the grazed area. In the protected area the vegetation cover, height and above- and belowground biomass increased significantly. Soil pH, electrical conductivity and bulk density decreased significantly, but soil organic carbon and total nitrogen concentration increased greatly in the protected area. By comparing the vegetation and soil characteristics with pre-degraded grassland, we found that vegetation can recover 6 years after fencing, and soil pH can be restored 8 years after fencing. However, the restoration of soil organic carbon, total nitrogen and total phosphorus concentrations needed 16, 30 and 19 years, respectively. It is recommended that the stocking rate should be reduced to 1/3 of the current carrying capacity, or that a grazing regime of 1-year of grazing followed by a 2-year rest is adopted to sustain the current status of vegetation and soil resources. However, if N fertilizer is applied, the rest period could be shortened, depending on the rate of application.

关键词： vegetation and soil restoration fencing grazing alkaline soil semi arid region grassland degradation

DNA-based immunization against hepatitis B surface antigen carrying preS epitopes

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Chinese Science Bulletin 1999年第7期44卷 620-623页

作者： Jingyi Hui guangdi li Yuying Kong Yuan Wang Chinese Acad Sci Shanghai Inst Biochem Shanghai 200031 Peoples R China

Three plasmid expression vectors containing modified hepatitis B surface antigen (HBsAg) carrying pres epitopes were constructed. Transient expression after in vitro transfection in COS-M6 cells showed that under the transcriptional control of the human cytomegalovirus (CMV) immediate early promoter, fusion genes expressed the modified HBV envelope proteins which were efficiently secreted into culture medium and presented HBsAg, preS1 and preS2 antigenicity. DNA-based immunization with these plasmids carrying pres sequences induced anti-HBs antibody in BALB/c mice. The titers of anti-HBs antibody were higher than those appeared in mice immunized with plasmid carrying S gene only. DNA injection with plasmids containing preS1 sequences elicited also high titers of anti-preS1 antibody. Moreover, the antipreS1 antibodies were found to appear earlier than anti-HBs antibodies.

关键词： preS epitopes hepatitis B surface antigen (HBsAg) DNA-based immunization antibody.

Effects of vegetation height and density on soil temperature variations

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Chinese Science Bulletin 2013年第8期58卷 907-912页

作者： SONG YanTao ZHOU DaoWei ZHANG HongXiang li guangdi JIN YingHua li Qiang Institute of Grassland Science Northeast Normal University Key Laboratory of Vegetation Ecology Ministry of Education Northeast Institute of Geography and Agroecology Chinese Academy of Sciences Graham Centre for Agricultural Innovation (alliance between Industry&Investment NSW and Charles Sturt University) Wagga Wagga Agricultural Institute

Reduction in vegetation cover caused by human activities has a great impact on soil temperature. It is important to assess how soil temperature responds to reduction of vegetation height and density. In this paper we first report the trends of mean annual soil surface and air temperatures recorded at the meteorological stations near the Ecological Research Station for Grassland Farming (ERSGF) from 1961 to 2007, then we setup an experiment using reed (Phragmites australis) stalks with different heights and densities to simulate effects of different vegetation height and density on soil and air temperatures. The warming rates of the mean annual soil and air temperatures were 0.043 and 0.041C a 1 , respectively. Changes of soil temperature were characterized by both increased mean annual maximum and minimum soil temperatures. At the experimental site, mean daily temperature, mean daily maximum soil and air temperatures increased significantly. In contrast, mean daily minimum soil temperature increased significantly while mean daily minimum air temperature decreased significantly as the height and density of reed stalks reduced during the experimental period. Mean diurnal soil temperature ranges were smaller than mean diurnal air temperature ranges. These results highlight that the importance of vegetation cover on soil and air temperatures.

关键词：土壤温度植被高度密度空气温度气温记录植被覆盖实验场地温度范围

Benefits of Conservation Agriculture on Soil and Water Conservation and Its Progress in China

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Agricultural Sciences in China 2011年第6期10卷 850-859页

作者： li ling-ling HUANG Gao-bao ZHANG Ren-zhi Bill Bellotti guangdi li Kwong Yin Chan Gansu Provincial Key Laboratory of Aridland Crop Science/Faculty of Agronomy Gansu Agricultural University Lanzhou 730070 P.R. China Faculty of Resource and Environment Gansu Agricultural University Lanzhou 730070 P.R.China School of Natural Sciences University of Western Sydney NSW 1797 Australia EH Graham Centre for Agricultural Innovation （Alliance Between Investment ＆ Industry NSW and Charles Sturt University） Wagga Wagga Agricultural Institute NSW 2650 Australia Investment ＆ Industry NSW NSW 2753 Australia

Conservation agriculture has been practised for three decades and has been spread widely. There are many nomenclatures surrounding conservation agriculture and differ to each other lightly. Conservation agriculture （CA） is a system approach to soil and water conservation, high crop productivity and profitability, in one word, it is a system approach to sustainable agriculture. Yet, because conservation agriculture is a knowledge-intensive and a complex system to learn and implement, and also because of traditions of intensive cultivation, adoption rates have been low, since to date, only about seven percent of the world＇s arable and permanent cropland area is farmed under conservation agriculture. The practice and wider extention of conservation agriculture thus requires a deeper understanding of its ecological underpinnings in order to manage its various elements for sustainable intensification, where the aim is to conserve soil and water and improve sustainability over the long term. This paper described terms related to conservation agriculture, presented the effects of conservation agriculture on soil and water conservation, crop productivity, progress and adoption of CA worldwide, emphasized obstacles and possible ways to increase CA adoption to accelerate sustainable development of China agriculture.

关键词： China conservation tillage crop residue mulch no-till soil and water conservation sustainability

limitation of fault-sealing and its control on hydrocarbon accumulation——An example from the Laoyemiao Oilfi eld of the Nanpu Sag

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Petroleum Science 2008年第4期5卷 295-301页

作者： Jiang Zhenxue Dong Yuexia li Hongyi liu Luofu liu guangdi li Xiaoying Basin and Reservoir Research Center China University of Petroleum Beijing 102249 China State Key Laboratory of Petroleum Resources and Prospecting China University of Petroleum Beijing 102249 China Research Institute of Exploration and Development Jidong Oilfield PetroChina Tangshan Hebei 063004 China School of Resources and Information Technology China University of Petroleum Beijing 102249 China

Based on previous studies on the internal structures of fault belts, the fault belts in the Laoyemiao Oilfield of the Nanpu Sag can be divided into three units, a crushed zone, an upper induced fracture zone and a lower induced fracture zone according to the log response characteristics. The upper induced fracture zone is characterized by the development of pervasive fractures and has a poor sealing or non-sealing capability. It therefore can act as pathways for hydrocarbon migration. The lower induced fracture zone consists of fewer fractures and has limited sealing capability. The crushed zone has a good sealing capability comparable to mudstone and can thus prevent lateral migration of fluid. Through physical modeling and comparing laboratory data with calculated data of oil column heights of traps sealed by faults, it is concluded that the fault-sealing capability for oil and gas is limited. When the oil column height reaches a threshold, oil will spill over from the top of reservoir along the lower induced fracture zone under the action of buoyancy, and the size of reservoir will remain unchanged. Analysis of the formation mechanisms of the fault-sealed reservoirs in the Nanpu Sag indicated that the charging sequence of oil and gas in the reservoir was from lower formation to upper formation, with the fault playing an important role in oil and gas accumulation. The hydrocarbon potential in reverse fault-sealed traps is much better than that in the consequent fault-sealed traps. The reverse fault-sealed traps are favorable and preferred exploration targets.

关键词： limitation of fault-sealing fault belt oil column height hydrocarbon accumulation Nanpu Sag

不同保护性耕作措施对旱作农田土壤水分的影响

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生态学报 2005年第9期25卷 2326-2332页

作者：李玲玲黄高宝张仁陟晋小军 guangdi li Kwong Yin CHAN 甘肃农业大学农业生态工程研究所兰州730070 甘肃农业大学资源与环境学院兰州730070 NSW Department of Primary Industry Wagga Wagga NSW 2650 Australia NSW Department of Primary Industry Richmond NSW 2753 Australia

研究了黄土高原西部旱农区传统耕作措施和五种保护性耕作措施对土壤水分垂直分布、动态变化、作物耗水量、水分利用效率及作物产量的影响.结果表明,不同保护性耕作措施对表层0～10cm土壤水分含量影响较大,免耕秸秆覆盖(NTS)在作物播种... 详细信息

研究了黄土高原西部旱农区传统耕作措施和五种保护性耕作措施对土壤水分垂直分布、动态变化、作物耗水量、水分利用效率及作物产量的影响.结果表明,不同保护性耕作措施对表层0～10cm土壤水分含量影响较大,免耕秸秆覆盖(NTS)在作物播种期可以显著增加播种期表层土壤含水量.0～200cm土壤剖面贮水量年变化分春夏作物旺盛生长失墒期(5月中旬～7月中旬)、夏秋雨季增墒期(7月中旬～10月下旬)和冬春稳墒期(11月～翌年5月上旬)3个阶段.尽管不同保护性耕作措施对0～200cm剖面贮水量影响不大,但作物耗水量却存在显著差异.免耕秸秆覆盖在增加作物耗水量的同时也提高了作物产量以及水分利用效率.地膜覆盖在有些年份也有利于降水的高效利用和作物产量的提高,但与秸秆覆盖相比不利于土壤肥力的持续提高.而且,如果种植小麦,出苗前若一次性降水较多时地膜覆盖将造成严重板结,影响出苗,进而降低产量和水分利用效率.因此,在黄土高原西部旱农区实施免耕+秸秆覆盖的保护性耕作措施,既有利于作物对有限降水的高效利用,提高作物产量,也可以促进农业可持续发展.

关键词：免耕秸秆覆盖地膜覆盖土壤水分水分利用效率

保护性耕作对旱地麦-豆双序列轮作农田土壤水分及利用效率的影响

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水土保持学报 2005年第3期19卷 165-169页

作者：郭清毅黄高宝 guangdi li YIN Chan 甘肃农业大学农学院甘肃兰州730070 NSW Department of Primary Industry Wagga WaggaNSW 2650Australia NSW Department of Primary Industry RichmondNSW 2753Australia

选择黄土高原陇中丘陵半干旱典型区———定西地区,在前期试验研究的基础上,2002～2004年通过对保护性耕作条件下旱地春小麦—豌豆双序列轮作农田土壤水分及利用效率的试验研究,结果表明:保护性耕作能够显著提高0～200cm土层土壤贮水量... 详细信息

选择黄土高原陇中丘陵半干旱典型区———定西地区,在前期试验研究的基础上,2002～2004年通过对保护性耕作条件下旱地春小麦—豌豆双序列轮作农田土壤水分及利用效率的试验研究,结果表明:保护性耕作能够显著提高0～200cm土层土壤贮水量及含水量,这种作用在降水较少年份表现更为突出。免耕及免耕覆草较常规耕作土壤贮水量分别提高1 93%～7 25%和0 06%～3 58%。休闲轮作有利于提高土壤对降水的保蓄,具有良好的蓄水保墒作用,但不同的轮作序列表现不同。P/W各处理小麦收获期土壤0～50cm含水量均比W/P各处理小麦收获期土壤0～50cm含水量有所增加,增幅为0 33%～0 62%。0～200cm土壤含水量呈反S型变化,播种期、五叶期及收获期土壤具有较高含水量,而开花期土壤含水量则较低。免耕覆草处理的产量及水分利用效率分别为3709 69kg/hm2和10 45kg/hm2·mm,分别较常规耕作提高30 28%和40 08%。

关键词：保护性耕作旱地轮作水分利用效率(WUE)

基于需求侧响应的区域综合能源系统的低碳经济调度

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高电压技术 2023年第3期49卷 1140-1149页

作者：何晓洋刘淼李健李广地张建辽宁电力交易中心有限公司沈阳110006 东北大学信息科学与工程学院沈阳110819

利用区域综合能源系统具有多能源互补及耦合的优势,能综合提升多种能源消纳的优化配置。提出一种区域综合能源系统的多目标低碳经济调度模型,充分挖掘区域能源系统内的储热装置、电锅炉和电储能促进风电并网消纳的优势。此外,综合考虑... 详细信息

利用区域综合能源系统具有多能源互补及耦合的优势,能综合提升多种能源消纳的优化配置。提出一种区域综合能源系统的多目标低碳经济调度模型,充分挖掘区域能源系统内的储热装置、电锅炉和电储能促进风电并网消纳的优势。此外,综合考虑电价弹性变化、温度变化的影响,建立了负荷弹性需求响应机制,降低了系统负荷的峰谷差水平,进一步提升风电的上网空间。最后,利用改进精英策略下的NSGA-Ⅱ算法对所提调度模型进行优化求解。通过仿真结果说明了考虑峰–谷分时电价及储热、储能和电锅炉装置均能提升风电进一步并网消纳的空间,有利于优化区域综合能源系统内的低碳调度指标。最后结合IEEE-39标准节点电力系统和一个7节点的热力系统验证了所提模型的合理性。

关键词：低碳调度需求响应分时电价改进精英策略风电消纳