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Ability of luteinizing hormone releasing hormone-Pseudomonas aeruginosa exotoxin 40 binding to LHRH receptor on human liver cancer cells

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World Journal of Gastroenterology 2004年第19期10卷 2870-2873页

作者： Shou-LiangGong GangZhao Hong-guangZhao Wen-TianLü guang-weiliu PingZhu RadiobiologyResearchUnitofMinistryofPublicHealth SchoolofPublicHealthJilinUniversityChangchun130021JilinProvinceChina InstituteofMilitaryVeterinaryMedicine QuartermasterUniversityofChinesePLAChangchun130062.JilinPrnvinceChina

AIM: TO explore the ability of recombinant toxin luteinizinghormone releasing hormone-Pseudomonas aeruginosaexotoxin 40 (LHRH-PE40) and LHRH binding to LHRH receptor(LHRHR) on the membrane surface of human liver cancerHEPG ***: LHRH was labeled by using 12sI with enzymaticreaction. The affinity and receptor volume of LHP, H-PE40and LHRH binding to LHRHR on the membrane surface ofhuman liver cancer cells were measured with radioligandreceptor ***: The specific activity of LHRH labeled with 12sIwas 2.7x 104 kBq/l^L, and its radiochemical purity reachedto 99.2-99.7%. The binding of 12sI to LHRH was maximalfor 240 min in the warm cultivation, and this binding wasstabilized. The inhibiting rates of ~2SI-LHRH and LHRH onthe proliferation of human liver cancer HEPG cells werenot significantly different. On the basis of the saturationcurve of ~2SI-LHRH binding to the membrane LHRHR of HEPGcells, ~2SI-LHRH of lx10s cpm was selected for radioligandreceptor assay. The affinity constants (Kd) of LHRH-PE40and LHRH binding to the membrane LHRHR of HEPG cellswere 0.43~0.12 nmol/L and 4.86~ 1.47 nmol/L, respectively,and their receptor volumes were 0.37~0.15 l^mol/g and0.42=I=0.13 l^mol/g, respectively. The binding of LHRH-PE40to the membrane protein of normal liver cells was ***: The recombinant toxin LHRH-PE40 binding tothe membrane surface of LHRHR of human liver cancer HEPGcells was very strong, while the specific binding of it to normalliver cells was not observed. The results provide an importantexperimental basis for the clinical application of LHRH-PE.

关键词：黄体激素激素释放荷尔蒙-假单胞激素铜绿色外毒素40 LHRH 粘合物受体肝细胞癌肿瘤

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Unraveling brain aging through the lens of oral microbiota

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Neural Regeneration Research 2025年第7期20卷 1930-1943页

作者： Qinchao Hu Si Wang Weiqi Zhang Jing Qu guang-Hui Liu State Key Laboratory of Membrane Biology Institute of ZoologyChinese Academy of SciencesBeijingChina Hospital of Stomatology Guanghua School of StomatologyGuangdong Provincial Key Laboratory of StomatologySun Yat-sen UniversityGuangzhouGuangdong ProvinceChina Advanced Innovation Center for Human Brain Protection and National Clinical Research Center for Geriatric Disorders Xuanwu HospitalCapital Medical UniversityBeijingChina Aging Translational Medicine Center International Center for Aging and CancerBeijing Municipal Geriatric Medical Research CenterXuanwu HospitalCapital Medical UniversityBeijingChina CAS Key Laboratory of Genomic and Precision Medicine Beijing Institute of GenomicsChinese Academy of Sciences and China National Center for BioinformationBeijingChina University of Chinese Academy of Sciences BeijingChina Institute for Stem Cell and Regeneration Chinese Academy of SciencesBeijingChina Aging Biomarker Consortium BeijingChina State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijingChina Beijing Institute for Stem Cell and Regenerative Medicine BeijingChina

The oral cavity is a complex physiological community encompassing a wide range of *** of oral microbiota can lead to various oral infectious diseases,such as periodontitis and tooth decay,and even affect systemic health,including brain aging and neurodegenerative *** studies have highlighted how oral microbes might be involved in brain aging and neurodegeneration,indicating potential avenues for intervention *** this review,we summarize clinical evidence demonstrating a link between oral microbes/oral infectious diseases and brain aging/neurodegenerative diseases,and dissect potential mechanisms by which oral microbes contribute to brain aging and *** also highlight advances in therapeutic development grounded in the realm of oral microbes,with the goal of advancing brain health and promoting healthy aging.

关键词： Alzheimer's disease brain aging multiple sclerosis neurodegeneration neurodegenerative diseases oral microbiota Parkinson's disease periodontitis bacteria Porphyromonas gingivalis

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Proteomics to display tissue repair opposing injury response to LPS-induced liver injury

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World Journal of Gastroenterology 2004年第18期10卷 2701-2705页

作者： Xiao-weiliu Fang-GenLu guang-SenZhang Xiao-PingWu YuYou Chun-HuiOuyang Dong-YeYang DepartmentofGastroenterology theSecondXiangyaHospitalCentralSouthUniversityChangsha410011HunanProvinceChina DepartmentofHematology theSecondXiangyaHospitalCentralSouthUniversityChangsha410011HunanProvinceChina

AIM: To examine the protein expression alterations in liver injury/repair network regulation as a response to gut-derived lipopolysaccharide (LPS) treatment, in order to anticipate the possible signal molecules or biomarkers in signaling LPS-related liver ***: Male BALB/c mice were treated with intraperitoneal (i.p.) LPS (4 mg/kg) and sacrificed at 0, 6, 24 and 30 h to obtain livers. The livers were stained with hematoxylin and eosin for histopathologic analyses. Total liver protein was separated by two-dimensional gel electrophoresis (2-DE). The peptide mass of liver injury or repair related proteins were drawn up and the protein database was searched to identify the ***: Observations were as follows: (1) TRAIL-R2 was down regulated in livers of LPS-treated mice. TNFAIP1 was significantly up regulated at 6 h, then down- regulated at 24, 30 h with silent expression during senescent stage.(2) The amount of metaxin 2 and mitochondria import inner membrane translocase subunit TIM8a (TIMM8A) was increased upon treatment with LPS, (3) P34 cdc2 kinase was significantly up-regulated 30 h after LPS administration with silent expression during senescent, 6, 24 h treated stage. (4) The amount of proteasome activator 28 alpha subunit (PA28), magnesium dependent protein phosphatase(MDPP) and lysophospholipase 2 was decreased 6 h after LPS treatment but recovered or up-regulated 24 and 30 h after LPS ***: LPS-treated mouse liver displaying a timedependent liver injury can result in expression change of some liver injury or repair related proteins.

关键词：组织修复免疫反应反作用 LPS-感应肝损伤脂多糖

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Association of HLA-DQ with Idiopathic Dilated Cardiomyopathy in a Northern Chinese Han Population

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Cellular & Molecular Immunology 2004年第4期1卷 311-314页

作者： weiliu WeiminLi NinglingSun DepartmentofCardiology FirstAffiliatedHospitalofHarbinMedicalUniversityHarbin150001China. DepartmentofCardiology People＇sHospitalofPekingUniversityBeijing100044China.

Autoimmune mechanisms are likely involved in the pathogenesis of idiopathic dilated cardiomyopathy (IDC) and components of MHC may serve as markers for the propensity to develop immune-mediated myocardial *** study was conducted to investigate the possible association between HLA-DQA1,-DQB1 alleles and IDC in Han population from northern China by using PCR-based sequence-specific primer (PCR-SSP) technique for HLA *** 68 unrelated IDC patients,4 probands of IDC pedigrees and 100 healthy controls,we found that the alleles of HLA-DQA1*0501 and HLA-DQB1*0303 conferred susceptibility to IDC while HLA-DQA1*0201 and HLA-DQB1*0502,*0504 alleles were in negative association with *** serine at position 57 (SER^(57)) in the exon of HLA-DQB1*0502 and *0504 was confirmed in our experiment as a marker for resistance to *** results suggest that HLA-DQ polymorphism may be involved in the pathogenesis of *** & Molecular Immunology.2004;1 (4):311-314.

关键词： idiopathic dilated cardiomyopathy HLA-DQA1 HLA-DQB1 polymorphism genetic susceptibility

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Dynamic Regulation of Hydrogen Bonding Networks and Solvation Structures for Synergistic Solar‑Thermal Desalination of Seawater and Catalytic Degradation of Organic Pollutants

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Nano-Micro Letters 2025年第2期17卷 548-565页

作者： Ming‑Yuan Yu Jing Wu guang Yin Fan‑Zhen Jiao Zhong‑Zhen Yu Jin Qu Beijing Key Laboratory of Advanced Functional Polymer Composites Beijing University of Chemical TechnologyBeijing 100029People’s Republic of China State Key Laboratory of Organic‑Inorganic Composites College of Materials Science and EngineeringBeijing University of Chemical TechnologyBeijing 100029People’s Republic of China

Although solar steam generation strategy is efficient in desalinating seawater,it is still challenging to achieve continuous solar-thermal desalination of seawater and catalytic degradation of organic ***,dynamic regulations of hydrogen bonding networks and solvation structures are realized by designing an asymmetric bilayer membrane consisting of a bacterial cellulose/carbon nanotube/Co_(2)(OH)_(2)CO_(3)nanorod top layer and a bacterial cellulose/Co_(2)(OH)_(2)CO_(3)nanorod(BCH)bottom ***,the hydrogen bonding networks inside the membrane can be tuned by the rich surface–OH groups of the bacterial cellulose and Co_(2)(OH)_(2)CO_(3)as well as the ions and radicals in situ generated during the catalysis ***,both SO_(4)^(2−)and HSO_(5)−can regulate the solvation structure of Na^(+)and be adsorbed more preferentially on the evaporation surface than Cl^(−),thus hindering the de-solvation of the solvated Na^(+)and subsequent nucleation/growth of ***,the heat generated by the solar-thermal energy conversion can accelerate the reaction kinetics and enhance the catalytic degradation *** work provides a flow-bed water purification system with an asymmetric solar-thermal and catalytic membrane for synergistic solar thermal desalination of seawater/brine and catalytic degradation of organic pollutants.

关键词： Solar steam generation Seawater desalination Catalytic degradation Bacterial cellulose Cobalt hydroxycarbonate nanorods

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Enhanced visible-light photocatalytic activity of a g-C_3N_4/m-LaVO_4 heterojunction: band offset determination

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Science Bulletin 2016年第8期61卷 645-655页

作者： Xiaoping Liu Hao Qin weiliu Fan School of Chemistry and Chemical Engineering Shandong University State Key Laboratory of Crystal Materials Shandong University

Band offset is a dominant factor affecting the photocatalytic performance of heterostructure photocatalysts. Therefore, controlling the band gap structure of semiconductors is a key challenge in the development of efficient photocatalysts. We used a typical in situ-method to prepare diverse graphite-phase carbon nitride(g-C_3N_4)samples from melamine, thiourea, and a mixture thereof,and found that they exhibited band gaps between2.3–2.8 e V. From UV–Vis spectra and X-ray photoelectron spectroscopy measurements, we determined that the g-C_3N_4 samples exhibited different band gap values and valence band positions. On this basis, we constructed g-C_3N_4/m-La VO_4 heterojunctions with different band offsets. UV–Vis spectra and X-ray photoelectron spectroscopy measurements revealed that the valence band offsets(VBOs) of the different heterojunctions were similar, but their conduction band offsets(CBOs) were significantly different. Photocatalytic experiments revealed that the reaction rate was enhanced with an increase in the CBO value. Furthermore, the three-phase g-C_3N_4/g-C_3N_4/mLa VO_4 heterojunction composed of m-La VO_4 and mixed g-C_3N_4 showed the highest photocatalytic activity, which was mainly due to the construction of a multilevel structure. This work investigates the influence of the band offset on heterojunction photoelectrochemical properties and provides a new strategy to improve the photocatalytic activity by constructing multilevel structures.

关键词： Carbon nitride.Visible light photocatalysis ~PrecursorsBand offset. MultilevelHeterojunction

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Electrochemical Studies on the Interactions of Dipyridophenazine Complex of Ruthenium and Herring Sperm DNA

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Chinese Chemical Letters 2005年第6期16卷 805-808页

作者： LiPingJIANG weiliu LingYahJIANG guangHartLU CollegeofChemistry CentralChinaNormalUniversityWuhan430079

Cyclic voltammetry (CV) and single-step chronocoulometry were used to study the interaction of [Ru(phen)2dppz]2+ (phen=1,10-phenanthroline; dppz=dipyrido[3,2-a:2′,3′-c]phen- azine) with herring sperm DNA. The addition of DNA caused a diminution in the peak current and a positive shift in the peak potential of the complex of [Ru(phen)2dppz]2+. The 12 mV positive shift in the peak potential of [Ru(phen)2dppz]2+ indicates that [Ru(phen)2dppz]2+ binds 2.6 times more strongly to DNA than its reductive form. In addition, by using fluorimetric and UV-spectrophotometric methods and studies of denatured DNA and the effect of NaCl solution, it was also found that the binding mode was intercalation. The decrease of peak current is proportional to the concentration of DNA, which can be applied to estimate DNA concentration.

关键词： Ru complex electrochemistry DNA.

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Lipopolysaccharide Could Be Internalized into Human Peripheral Blood Mononuclear Cells and Elicits TNF-α Release,but not via the Pathway of Toll-Like Receptor 4 on the Cell Surface

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Cellular & Molecular Immunology 2004年第5期1卷 373-377页

作者： HongZhou GuofuDing weiliu LiangxiWang YonglingLu HongweiCao JiangZheng DepartmentofPharmacology CollegeofMedicineThirdMilitaryMedicalUniversityChongqing400038China. MedicalResearchCenter SouthwesternHospitalThirdMilitaryMedicalUniversityChongqing400038China.

Lipopolysaccharide(LPS),the principal component of the outer membrane of Gram-negative bacteria,stimulates various cell types to release numerous proinflammatory mediators such as TNF-α,IL-6 and IL-12,which may damage cells and lead to organ injury,even sepsis and septic ***-like receptor 4(TLR4) has been identified as the receptor involved in the recognition of LPS,but the role of LPS uptake in activating signal transduction remains *** the present study,TNF-α was used as a marker of macrophages/ monocytes activated by LPS,and CQ was used as an inhibitor of endosome mature in order to definitude what stage of the signal transduction elicited by LPS was *** found that there indeed existed internalization of LPS and internalization partially participated in LPS signaling since CQ inhibited cytokine release,and decreased accumulation of FITC-LPS in *** contrast,anti-hTLR4 antibody could decrease cytokine release,but had no inhibition on accumulation of *** result revealed that inhibition of cytokine release was related to reduction of FITC-LPS accumulation in the *** TLR4 on the cell surface couldn't participate in internalization of ***,LPS signaling and internalization couldn't be viewed as mutually independent *** & Molecular Immunology.2004;1(5):373-377.

关键词： LPS TNF-α TLR4 internalization CQ

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Determination of Surface pKa of Pure Mercaptoacetic Acid and 2-Mercaptobenzothiazole Mixed Monolayers by Impedance Titration

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Chinese Chemical Letters 2004年第7期15卷 827-830页

作者： guangHanLU ChuanYinLIU HongYanZHAO weiliu LiPingJIANG LingYanJIANG Collegeofchemistry CentralChinaNormalUniversityWuhan430079 DepartmentofChemistry YunyangTeacher＇sCollegeDanjiangkouHubei442700

Interfacial proton transfer reactions of pure mercaptoacetic acid (MA) and 2-mercaptobenzothiazole (Mbz) mixed self-assembled monolayers (SAMs) have been studied using a.c. impedance titration method. The charge-transfer resistance (Rct,) is measured with the monolayer composition and the ionic strength of pH solution. The surface pKa can be obtained by the plots of Rct and pH, the reasons of shifts of surface pKa are also explained.

关键词： A.c.impedance titration mercaptoacetic acid 2-mercaptobenzothiazole self-assembled monolayers surface pKa.

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Multiple gene differential expression patterns in human ischemic liver:Safe limit of warm ischemic time

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World Journal of Gastroenterology 2004年第14期10卷 2130-2133页

作者： Qi-PingLu Ting-]iaCao Zhi-YongZhang weiliu DepartmentofGeneralSurgery WuhanGeneralHospitalofGuangzhouMilitaryCommandWuhan430070HubeiProvinceChina

AIM: To investigate the multiple gene differential expression pattems in human ischemic liver and to produce the evidence about the hepatic ischemic safety ***: The responses of cells to hepatic ischemia and hypoxia at hepatic ischemia were analyzed by cDNA microarrary representing 4 000 different human genes containing 200 apoptotic correlative genes. RESULTS: There were lower or normal expression levels of apoptotic correlative genes during the periods of hepatic ischemia for 0-15 min, the maintenance homostatic genes were expressed significantly higher at the same time. But at the hepatic ischemia for 30 min, the expression levels of maintenance homeostatic genes were down-regulated, the expressions of many apoptotic correlative genes and nuclear transcription factors were activated and ***: HIF-1, APAF-1, PCDC10, FBX5, DFF-40, DFFA XIAP, survivin may be regarded as the signal genes to judge the degree of hepatic ischemic-hypoxic injure, and the apoptotic liver cell injury due to ischemia in different time limits. The safe limit of human hepatic warm ischemic time appears to be generally less then 30 min.

关键词：基因多样性基因表达表达模式局部缺血性肝脏缺血性疾病肝损害

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