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Gene cloning,protein expression,and enzymatic characterization of a double-stranded RNA degrading enzyme in Apolygus lucorum

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Insect Science 2024年第1期31卷 119-133页

作者： Jie-Yu Zhang Jing Zhao Keyan Zhu-Salzman Qin-Qin Ji Yi-Ping Jiang Liu-Bin Xiao De-Jin xu guang-chun xu Lin-Quan Ge Yong-An Tan College of Plant Protection Yangzhou UniversityYangzhouJiangsu ProvinceChina Institute of Plant Protection Jiangsu Academy of Agricultural SciencesNanjingJiangsu ProvinceChina Department of Entomology Texas A&M UniversityCollege StationTXUSA Taizhou Customs of the People's Republic of China TaizhouJiangsu ProvinceChina

RNA interference(RNAi)is a powerful tool that post-transcriptionally silences target genes in eukaryotic ***,silencing efficacy varies greatly among different insect ***,we met with little success when attempting to knock down genes in the mirid bug Apolygus lucorum via dsRNA *** disappearance of double-stranded RNA(dsRNA)could be a potential factor that restricts RNAi ***,we found that dsRNA can be degraded in midgut fluids,and a dsRNase of ***(AldsRNase)was identified and *** alignment indicated that its 6 key amino acid residues and the Mg2+-binding site were similar to those of other insects’*** signal peptide and endonuclease non-specific domain shared high sequence identity with the brown-winged green stinkbug Plautia stali *** showed high salivary gland and midgut expression and was continuously expressed through the whole life cycle,with peaks at the 4th instar ecdysis in the whole *** purified AldsRNase protein obtained by heterologously expressed can rapidly degrade *** comparing the substrate specificity of AldsRNase,3 specific substrates(dsRNA,small interfering RNA,and dsDNA)were all degraded,and the most efficient degradation is ***,immunofluorescence revealed that AldsRNase was expressed in the cytoplasm of midgut *** cloning and functional study of AldsRNase,the enzyme activity and substrate specificity of the recombinant protein,as well as the subcellular localization of nuclease,the reason for the disappearance of dsRNA was explained,which was useful in improving RNAi efficiency in *** and related species.

关键词： Apolygus lucorum cloning dsRNase enzymatic activity protein expression

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Gluing Affine Vortices

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Acta Mathematica Sinica,English Series 2024年第1期40卷 250-312页

作者： guang Bo xu Department of Mathematics Rutgers UniversityHill Center-Busch Campus 110 Frelinghuysen RoadPiscatawayNJ 08854-8019USA

We provide an analytical construction of the gluing map for stable affine vortices over the upper half plane with the Lagrangian boundary *** result is a necessary ingredient in studies of the relation between gauged ... 详细信息

关键词： Affine vortices gauged linear sigma model adiabatic limit symplectic reduction

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Heat transfer and plume statistics in turbulent thermal convection with sparse fractal roughness

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Journal of Hydrodynamics 2021年第5期33卷 1065-1077页

作者： guang-chun xu Qi Wang Zhen-hua Wan De-jun Sun Department of Modern Mechanics University of Science and Technology of ChinaHefei 230027China

Rough-surface Rayleigh-Benard convection is investigated using direct numerical simulations in two-dimensional convection cells with aspect ratioГ=*** types of fractal roughness elements,which are marked as nl,n2 and n3,are constructed based on the Koch curve and sparsely mounted on both the plates,where n denotes the level of the *** considered Rayleigh numbers Ra range from 10^(7)to 10^(11)with Prandtl number Pr=*** regimes are identified for cases nl,*** Regime I,the scaling exponentsβin the effective Nusselt number Nu vs Ra scaling Nu~Ra^(β)reach up to about ***,when Ra is larger than a critical value Ra_(c),the flow enters RegimeⅡ,with p saturating back to a value close to the smooth-wall case(0.3).Rac is found to increase with increasing n,and for case n3,only Regime I is identified in the studied Ra *** extension of Regime I in case n3 is due to the fact that at high Ra,the smallest roughness elements can play a role to disrupt the thermal boundary *** thermal dissipation rate is studied and it is found that the increasedβin Regime I is related with enhanced thermal dissipation rate in the *** interesting finding is that no clear convection roll structures can be identified for the rough cases,which is different from the smooth case where well-organized convection rolls can be *** difference is further quantified by the detailed analysis of the plume statistics,and it is found that the horizontal profiles of plume density and velocity are relatively flattened due to the absence of clear convection rolls.

关键词： Thermal convection Rayleigh-Benard convection rough surface Introduction

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Breaking the efficiency limitations of dissipative Kerr solitons using nonlinear couplers

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Science China(Physics,Mechanics & Astronomy) 2024年第3期67卷 88-94页

作者： Ming Li Xiao-Xiao xue Yan-Lei Zhang Xin-Biao xu chun-Hua Dong guang-Can Guo Chang-Ling Zou CAS Key Laboratory of Quantum Information University of Science and Technology of ChinaHefei 230026China CAS Centerfor Excellence in Quantum Information and Quantum Physics University of Science and Technology of ChinaHefei 230026China Department of Electronic Engineering Beijing National Research Center for Information Science and TechnologyTsinghua University Beijing 100084China

Dissipative Kerr solitons(DKS) have long been suffering from poor power conversion efficiency when driven by continuous-wave lasers. By deriving the critical coupling condition of a multimode nonlinear optics system in a generalized theoretical framework,two efficiency limitations of the conventional pump method of DKS are revealed: the effective coupling rate is too small and is also power-dependent. A general approach is provided to resolve this challenge by introducing two types of nonlinear couplers to couple the soliton cavity and CW input through nonlinear processes. The collective coupler opens multiple coupling channels and the self-adaptive coupler builds a power-independent effective external coupling rate to the DKS for approaching the generalized critical coupling condition, which promises near-unity power conversion efficiencies. For instance, a conversion efficiency exceeding 90% is predicted for aluminum nitride microrings with a nonlinear coupler utilizing second-harmonic generation. The mechanism applies to various nonlinear processes, including Raman and Brillouin scattering, and thus paves the way for micro-solitons toward practical applications.

关键词： Kerr frequency comb conversion efficiency nonlinear coupler

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An efficient method to synthesize HMX by nitrolysis of DPT with N_2O_5 and a novel ionic liquid

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Chinese Chemical Letters 2009年第4期20卷 379-382页

作者： Hui Zhen Zhi Jun Luo guang An Feng chun xu Lv School of Chemical Engineering Nanjing University of Science and Technology Nanjing 210094 China

A novel polyethylene glycol （PEG）-200-based dicationic acidic ionic liquid （PEG200-DAIL） was used to synthesize HMX from DPT by nitrolysis with N2O5. It was found that either N2O5 or PEGzoo-DAIL could improve the yield. Furthermore, the combined use of PEG200-DAIL and N2O5 could increase the yield of HMX to 64% with the used quantity of HNO3 decreased dramatically.

关键词： DPT Nitrolysis N2O5 Acidic ionic liquid

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Stimulated Brillouin interaction between guided phonons and photons in a lithium niobate waveguide

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Science China(Physics,Mechanics & Astronomy) 2024年第1期67卷 173-175页

作者： Yuan-Hao Yang Jia-Qi Wang Zheng-xu Zhu Xin-Biao xu Qiang Zhang Juanjuan Lu Yu Zeng chun-Hua Dong Luyan Sun guang-Can Guo Chang-Ling Zou CAS Key Laboratory of Quantum Information University of Science and Technology of ChinaHefei 230026China CAS Center for Excellence in Quantum Information and Quantum Physics University of Science and Technology of ChinaHefei 230026China Key Laboratory of Quantum Optics and Quantum Optics Devices Institute of Opto-ElectronicsShanxi UniversityTaiyuan 030006China School of Information Science and Technology ShanghaiTech UniversityShanghai 201210China Center for Quantum Information Institute for Interdisciplinary Information SciencesTsinghua UniversityBeijing 100084China

The Brillouin scattering is one of the strongest nonlinear optical effects in dielectrics[1].In particular,the backward Brillouin scattering(BBS)provides an efficient method to achieve low-noise optical gain for active photonics[2]and the coherent interconnects between 10 GHz phonons and photons[3].However,previous studies on BBS are primarily limited to the unconfined phonons[1]or phonon confined in suspended structures[4-6].

关键词： scattering waveguide Brillouin

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Electromagnetic fields in ultra-peripheral relativistic heavy-ion collisions

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Nuclear Science and Techniques 2024年第2期35卷 103-109页

作者： Jie Zhao Jin-Hui Chen xu-guang Huang Yu-Gang Ma Key Laboratory of Nuclear Physics and Ion-beam Application(MOE) Institute of Modern PhysicsFudan UniversityShanghai 200433China Shanghai Research Center for Theoretical Nuclear Physics NSFC and Fudan UniversityShanghai 200438China Physics Department and Center for Particle Physics and Field Theory Fudan UniversityShanghai 200438China

Ultra-peripheral heavy-ion collisions(UPCs)offer unique opportunities to study processes under strong electromagnetic *** these collisions,highly charged fast-moving ions carry strong electromagnetic fields that can be effectively treated as photon *** exchange of photons can induce photonuclear and two-photon interactions and excite *** excitation of the ions results in Coulomb dissociation with the emission of photons,neutrons,and other ***,the electromagnetic fields generated by the ions can be sufficiently strong to enforce mutual interactions between the two colliding ***,the two colliding ions experience an electromagnetic force that pushes them in opposite directions,causing a back-to-back correlation in the emitted *** a Monte Carlo simulation,we qualitatively demonstrate that the above electromagnetic effect is large enough to be observed in UPCs,which would provide a clear means to study strong electromagnetic fields and their effects.

关键词： Electromagnetic fields Neutrons Ultra-peripheral relativistic heavy-ion collisions(UPC)

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Applying deep learning technique to chiral magnetic wave search

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Chinese Physics C 2024年第8期48卷 170-176页

作者： Yuan-Sheng Zhao xu-guang Huang Physics Department and Center for Particle Physics and Field Theory Fudan UniversityShanghai 200433China Key Laboratory of Nuclear Physics and Ion-beam Application(MOE) Fudan UniversityShanghai 200433China Shanghai Research Center for Theoretical Nuclear Physics National Natural Science Foundation of China and Fudan UniversityShanghai 200438China

The chiral magnetic wave(CMW)is a collective mode in quark-gluon plasma originated from the chiral magnetic effect(CME)and chiral separation *** detection in heavy-ion collisions is challenging owing to significant background ***[*** et al.,***.C 106,L051901(2022)],we constructed a neural network that accurately identifies the CME-related signal from the final-state pion *** this study,we have generalized this neural network to the case of CMW *** show that,after an updated training,the neural network effectively recognizes the CMW-related ***,we have assessed the performance of the neural network in comparison with other known methods for CMW search.

关键词： deep learning chiral magnetic wave relativistic heavy-ion collisions

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Context Processing in Contextual and Cued Fear Extinction

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Neuroscience Bulletin 2024年第6期40卷 835-839页

作者： Yimu Zhang chun xu Yu Gu State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science Institutes of Brain ScienceFudan UniversityShanghai200032China Institute of Neuroscience State Key Laboratory of NeuroscienceCenter for Excellence in Brain Science and Intelligence TechnologyChinese Academy of SciencesShanghai200031China

Anxiety disorders such as post-traumatic stress disorder(PTSD)are prevalent mental disorders that disturb millions *** disorders often manifest as excessive fear,anxiety,and distress,significantly impacting daily *** fear extinction paradigms in animals were established decades ago and have been widely used in research,providing a scientific basis for exposure therapies for anxiety disorders in humans.

关键词： distress disorders traumatic

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Transverse mode-coupling instability with longitudinal impedance

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Nuclear Science and Techniques 2024年第6期35卷 40-53页

作者： Hai-Sheng xu chun-Tao Lin Na Wang Jing-Ye xu Yuan Zhang Key Laboratory of Particle Acceleration Physics and Technology Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China University of Chinese Academy of Sciences Beijing 100049China Institute of Advanced Science Facilities Shenzhen 518107GuangdongChina

Transverse mode-coupling instability(TMCI)is a dangerous transverse single-bunch instability that can lead to severe par-ticle *** mechanism of TMCI can be explained by the coupling of transverse coherent oscillation modes owing to the transverse short-range wakefield(i.e.,the transverse broadband impedance).Recent studies on future circular colliders,e.g.,FCC-ee,showed that the threshold of TMCI decreased significantly when both longitudinal and transverse impedances were *** performed computations for the circular electron-positron collider(CEPC)and observed a similar *** studies on the influence of longitudinal impedance on the TMCI threshold were *** concluded that the imaginary part of the longitudinal impedance,which caused a reduction in the incoherent synchrotron tune,was the primary reason for the reduction in the TMCI ***,the real part of the longitudinal impedance assists in increasing the TMCI threshold.

关键词： Transverse mode-coupling instability Longitudinal impedance

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