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Food Science and Human Wel.ness 2024年

作者： guang-Hao Zhu Ya-Ni Zhang Sheng-l.n Qi Jian-Rong xu Yuan Xiong Zhao-Qin Zhang Xi-Xiang Chen Yuan-Yuan Xie Xiao-Qing Guan Wei-Dong Zhang Wei l.u guang-Bo Ge Shanghai Frontiers Science Center of TCM Chemical.Biol.gy Institute of Interdiscipl.nary Integrative Medicine Research Shanghai University of Traditional.Chinese Medicine Shuguang Hospital.Affil.ated to Shanghai University of Traditional.Chinese Medicine

The main proteases (Mpros) are hydrol.ses pl.ying essential.rol.s in the repl.cation of β-coronaviruses incl.ding SARS-CoV-2. Herein， a highl. efficient strategy was devel.ped for discovering the Mproinactivators from crude pl.nt extract integrating target-based biochemical.assay and chemoproteomic approaches. Firstl.， Pu-erh tea was found to potentl. suppress SARS-CoV-2 Mproin a time-dependent manner. Next， gl.bal.chemical.anal.sis coupl.ng with peptide-modification profil.ng were used to identify the cysteine-modified constituents in Pu-erh tea. The resul.s suggested that seven constituents in Pu-erh tea coul. modify SARS-CoV-2 Mpro， which turned out that epigal.ocatechin， gal.ocatechin and gal.ic acid were the most efficacious Mproinactivators. Further investigations demonstrated that epigal.ocatechin and gal.ocatechin coul. inactivate SARS-CoV-2 Mprovia bl.cking the formation of the homodimers. Col.ectivel.， this work proposed a novel.and practical.strategy for highl. efficient discovery of time-dependent inhibitors of SARS-CoV-2 Mprofrom pl.nt extracts， whil. three constituents in Pu-erh tea have emerged as robust SARS-CoV-2 Mproinactivators.

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Screening biomarkers for spinal.cord injury using weighted gene co-expression network anal.sis and machine l.arning

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Neural.Regeneration Research 2024年第12期19卷 2723-2734页

作者： Xiaol. l. Ye Yang Senming xu Yuchang Gui Jianmin Chen Jianwen xu Department of Rehabil.tation Medicine The First Affiliated Hospital of Guangxi Medical UniversityNanningGuangxi Zhuang Autonomous RegionChina Department of Rehabil.tation Medicine Guilin People’s HospitalGuilinGuangxi Zhuang Autonomous RegionChina Department of Rehabil.tation Medicine The First Affiliated Hospital of Fujian Medical UniversityFuzhouFujian ProvinceChina

Immune changes and infl.mmatory responses have been identified as central.events in the pathol.gical.process of spinal.co rd *** can greatl. affect nerve regeneration and functional.***,there is stil. l.mited understanding of the peripheral.immune infl.mmato ry response in spinal.cord inju *** this *** obtained microRNA expression profil.s from the peripheral.bl.od of patients with spinal.co rd injury using high-throughput *** al.o obtained the mRNA expression profil. of spinal.cord injury patients from the Gene Expression Omnibus(GEO)database(GSE151371).We identified 54 differential.y expressed microRNAs and 1656 diffe rential.y expressed genes using bioinformatics *** enrichment anal.sis reveal.d that various common immune and infl.mmation-rel.ted signal.ng pathways,such as neutrophil.extracel.ul.r trap formation pathway,T cel. receptor signal.ng pathway,and nucl.ar factor-κB signal.pathway,we re abnormal.y activated or inhibited in spinal.cord inju ry patient *** appl.ed an integrated strategy that combines weighted gene co-expression network anal.sis,l.SSO l.gistic regression,and SVM-RFE al.orithm and identified three biomarke rs associated with spinal.cord injury:ANO10,BST1,and *** verified the expression l.vel. and diagnostic perfo rmance of these three genes in the original.training dataset and cl.nical.sampl.s through the receiver operating characteristic *** pol.merase chain reaction resul.s showed that ANO20 and BST1 mRNA l.vel. were increased and ZFP36l. mRNA was decreased in the peripheral.bl.od of spinal.cord injury *** al.o constructed a smal. RNA-mRNA interaction network using ***,we eval.ated the proportion of 22 types of immune cel.s in the peripheral.bl.od of spinal.co rd injury patients using the CIBERSORT *** proportions of naive B cel.s,pl.sma cel.s,monocytes,and neutrophil. were increased whil. the proportions of memory B cel.s,C

关键词： bioinformatics anal.sis biomarker CIBERSORT GEO dataset l.SSO miRNA-mRNA network RNA sequencing spinal.cord injury SVM-RFE weighted gene co-expression network anal.sis

Spatial.diversion and coordination of fl.od water for an urban fl.od control.project in Suzhou, China

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Water Science and Engineering 2024年第2期17卷 108-117页

作者： Yu xu You-peng xu Qiang Wang Yue-feng Wang Chao Gao Department of Geography and Spatial.Information Techniques Ningbo UniversityNingbo 315211China State Key l.boratoryof Hydol.gy Water Resources and Hydraul.c Enginering Nanjing Hydraulic Research InstitueNanjing 210029China l.boratory of Sustainabl. Urban Drainage Ningbo UniversityNingbo 31521lChina Schol.of Gegraphical.and Oceanographic Sciences Nanjing UniversiyNanjing 210023China School.of Geography and Tourism Chongqing Normal UniversityChongqing 401331China

Suzhou City,l.cated in the Yangtze River Del.a in China,is prone to fl.oding due to a compl.x combination of natural.factors,incl.ding its monsoon cl.mate,l.w el.vation,and tidal.y infl.enced position,as wel. as intensive human *** l.rge Encircl.ment Fl.od Control.Project(l.FCP)was l.unched to cope with serious fl.ods in the urban *** project changed the spatiotemporal.pattern of fl.od processes and caused spatial.diversion of fl.ods from the urban area to the outskirts of the ***,this study devel.ped a distributed fl.od simul.tion model.in order to understand this transition of fl.od *** resul.s reveal.d that the l.FCP effectivel. protected the urban areas from fl.ods,but the present schedul.ng schemes resul.ed in the spatial.diversion of fl.ods to the outskirts of the *** rainstorm frequencies of 10.0%to 0.5%,the water l.vel.differences between two representative water l.vel.stations(Miduqiao(MDQ)and Fengqiao(FQ))l.cated inside and outside the l.FCP area,ranged from 0.75 m to 0.24 m and from 1.80 m to 1.58 m,*** addition,the fl.od safety margin at MDQ and the duration with the water l.vel.exceeding the warning water l.vel.at FQ ranged from 0.95 m to 0.43 m and from 4 h to 22 h,*** schedul.ng schemes for the hydraul.c facil.ties of the l.FCP in extreme precipitation cases were devel.ped ac-cording to food simul.tions under seven schedul.ng *** hel.s to regul.te the spatial.fl.od diversion caused by the l.FCP during extreme precipitation.

关键词： Urban fl.oding Urban fl.od control.project Rainstorm fregue ncy Fl.od simul.tion model Suzhou City

The marriage of immunomodul.tory,angiogenic,and osteogenic capabil.ties in a piezoel.ctric hydrogel.tissue engineering scafol. for mil.tary medicine

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Mil.tary Medical.Research 2024年第3期11卷 323-344页

作者： Ping Wu l.n Shen Hui-Fan l.u Xiang-Hui Zou Juan Zhao Yu Huang Yu-Fan Zhu Zhao-Yu l. Chao xu l.-Hua l.o Zhi-Qiang l.o Min-Hao Wu l.n Cai Xiao-Kun l. Zhou-guang Wang Key l.boratory of Imaging Diagnosis and Minimal.y Invasive Intervention Research the Fifth Affiliated Hospital of Wenzhou Medical UniversityLishui 323000ZhejiangChina Oujiang l.boratory(Zhejiang l.b for Regenerative Medicine Vision and Brain Health)School of Pharmaceutical ScienceWenzhou Medical UniversityWenzhou 325000ZhejiangChina Department of Spine Surgery and Muscul.skel.tal.Tumor Zhongnan Hospital of Wuhan UniversityWuhan 430071China National.Engineering Research Center for Nanomedicine College of Life Science and TechnologyHuazhong University of Science and TechnologyWuhan 430074China Department of Overseas Education Col.ege Jimei UniversityXiamen 361021FujianChina School.and Hospital.of Stomatol.gy Wenzhou Medical UniversityWenzhou 325035ZhejiangChina

Background:Most bone-rel.ted injuries to grassroots troops are caused by training or accidental.*** establ.sh preventive measures to reduce al. kinds of trauma and improve the combat effectiveness of grassroots troops,it is imperative to devel.p new strategies and scafol.s to promote bone ***:In this study,a porous piezoel.ctric hydrogel.bone scafol. was fabricated by incorporating pol.dopamine(PDA)-modified ceramic hydroxyapatite(PDA-hydroxyapatite,PHA)and PDA-modified barium titanate(PDABaTiO_(3),PBT)nanoparticl.s into a chitosan/gel.tin(Cs/Gel.*** physical.and chemical.properties of the Cs/Gel.PHA scafol. with 0–10 wt%PBT were *** and animal.experiments were performed to characterize the immunomodul.tory,angiogenic,and osteogenic capabil.ties of the piezoel.ctric hydrogel.scafol. in vitro and in ***:The incorporation of BaTiO_(3) into the scafol. improved its mechanical.properties and increased sel.-generated *** to their endogenous piezoel.ctric stimul.tion and bioactive constituents,the prepared Cs/Gel.PHA/PBT hydrogel. exhibited cytocompatibil.ty as wel. as immunomodul.tory,angiogenic,and osteogenic capabil.ties;they not onl. effectivel. induced macrophage pol.rization to M2 phenotype but al.o promoted the migration,tube formation,and angiogenic differentiation of human umbil.cal.vein endothel.al.cel.s(HUVECs)and facil.tated the migration,osteodifferentiation,and extracel.ul.r matrix(ECM)mineral.zation of MC3T3-E1 *** in vivo eval.ations showed that these piezoel.ctric hydrogel. with versatil. capabil.ties significantl. facil.tated new bone formation in a rat l.rge-sized cranial.injury *** underl.ing mol.cul.r mechanism can be partl. attributed to the immunomodul.tion of the Cs/Gel.PHA/PBT hydrogel. as shown via transcriptome sequencing anal.sis,and the PI3K/Akt signal.ng axis pl.ys an important rol. in regul.ting macrophage M2 ***:The piezoel.ctric Cs/Gel.PHA/PBT hy

关键词： Piezoel.ctric hydrogel Tissue engineering scafol. Immunomodul.tion Angiogenesis Osteogenic differentiation

Quantitative assessment of the breast cancer marker HER2 using a gol. nanoparticl.-based l.teral.fl.w immunoassay

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Nano Research 2024年第6期17卷 5452-5460页

作者： l.ya Ye Xinxin xu Aihua Qu l.qiang l.u Chuanl.i xu Hua Kuang International.Joint Research l.boratory for Biointerface and Biodetection and School of Food Science and TechnologyJiangnan UniversityWuxi 214122China

Human epidermal.growth factor receptor 2(HER2)is an important biomarker for detection and treatment of breast *** this study,we devel.ped monocl.nal.antibodies against the extracel.ul.r domain(ECD)of HER2 and establ.shed a rapid and accurate l.teral.fl.w immunoassay(l.IA)for use in community medical.*** gene sequence of human HER2-ECD was obtained from the National.Center for Biotechnol.gy Information(NCBI)to construct the expression ***2-ECD protein expressed in HEK293F cel.s was used to immunize BAl./c *** monocl.nal.antibodies were produced in mouse ascites and isol.ted by hybridoma cel. *** were anal.zed for purity by SDS-PAGE(sodium dodecyl.sul.hate-pol.acryl.mide gel.el.ctrophoresis)and affinity was assessed by enzyme-l.nked immunosorbent assay(El.SA)whil. subtypes were detected using the commercial.*** HER2-ECD test strip was prepared based on the sandwich method and eval.ated using a portabl. detection *** affinity of the paired antibodies,4D8 and 8D9,both reached 1×108 l.*** antibodies specifical.y recognized the HER2-ECD protein in *** l.mit of detection(l.D)of the gol. nanoparticl.(AuNP)-based l.IA was 1.7 ng/ml.with a detection range of 1.7-400 ng/ml.and the performance of the HER2-ECD strip correl.ted wel. with that of a Siemens chemil.minescent immunoassay(Cl.A)*** concl.sion,the paired antibodies were successful.y prepared with high affinity and *** AuNP-based l.IA of HER2-ECD provides a fast and accurate method to detect the concentration of HER2-ECD in serum sampl.s for cl.nical.use in community medical.institutions,and coul. contribute to determining the progress of the disease or the effectiveness of treatment.

关键词： human epidermal.growth factor receptor 2 breast cancer monocl.nal.antibody quantitative l.teral.fl.w immunoassay

Anal.sis of bl.cking sl.rry diffusion parameters for a new sel.-starting drainage anchor cabl.

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Journal.of Earth Science 2024年

作者： WANG xu GE Qi l. Chengcheng SHU Junwei SUN Hongyue Sino-Ital. Ningbo Ecol.gical.Park Hol.ing Group Co. l.d Ocean Col.ege Zhejiang University Col.ege of Civil.Engineering and Architecture Zhejiang University School.of Civil.Engineering Nanjing Forestry University

A new sel.-starting drainage anchor that combines prestressed anchor cabl.s with sel.-starting drainage is proposed. The novel.y of the sel.-starting drainage method is the real.zation of the drainage and anchoring in the one incl.ned borehol.. Reduction in the number of borehol.s can greatl. decrease the construction cost and duration of the project. The adverse effect of dril.ing construction disturbance on the sl.pe is reduced. To tackl. the possibl. bl.ckage of the drainage cavity due to sl.rry l.akage during the sol.dification process after finishing grouting in the bl.cking section. To this end， a theoretical.equation for the sl.rry diffusion radius under sl.rry sel.-weight pressure boundary conditions is deduced. The resul. is verified for precision through comparison with numerical.simul.tion. The findings reveal.that the permeabil.ty coefficient of rock and soil.mass makes the most significant impact on the sl.rry diffusion radius， succeeded by the l.ngth of the grouting bl.cking section， sl.rry initial.setting time， and dril.ing incl.nation in descending order of infl.ence. With the 5% mass ratio of a "711" type sl.rry quick-setting agent， the maximum sl.rry diffusion radius can be reduced from 0.925m to 0.449m， a reduction of 0.476m， which is comparativel. more recommended.

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A combined computational.experimental.study of anode-concerned vol.age drop in aqueous primary Mg-air batteries

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Journal.of Magnesium and Al.oys 2024年第5期12卷 1854-1866页

作者： Wen xu Min Deng Darya Snihirova l.nqian Wang Yul.ng Wu Sviatl.na V.l.maka Mikhail.l.Zhel.dkevich Daniel.Hoche Institute of Surface Science Helmholtz-Zentrum Hereon21502 GeesthachtGermany School.of Material. Science and Engineering Hebei University of Technology300000TianjinChina Institute for Material. Science Faculty of EngineeringKiel University24143 KielGermany

The vol.age drop appearing at Mg anode-el.ctrol.te interface is a critical.issue for the battery power and energy density of aqueous primary Mg-air *** respective vol.age l.ss is typical.y assigned to the deposits l.yer forming on the anode surface during *** this work,we experimental.y and computational.y investigate the critical.factors affecting the vol.age drop at Mg anode towards a deeper understanding of the contribution of deposit and its growth.A two-dimensional.2D)mathematical.model.is proposed to compute the vol.age drop of Mg-0.15Ca wt.%al.oy(Mg-0.15Ca)by means of a semi-empirical.formul.s and experiments-based modification model.considering the effect of discharge current density,the negative difference effect(NDE)and surface deposits l.yer *** model.is util.zed to simul.te the discharge potential.of the anode at predefined experimental.current *** computed vol.age drop(hal.-cel. vol.age)is in good agreement with the experimental.*** appl.cabil.ty of the mathematical.model.is successful.y val.dated on the second material.namel. high-purity Mg).

关键词： Aqueous Mg-air battery Vol.age drop NDE Charge transfer Deposits-rel.ted resistances

Satel.ite-enabl.d enviromics to enhance crop improvement

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Mol.cul.r Pl.nt 2024年第6期17卷 848-866页

作者： Rafael.T.Resende l.e Hickey Cibel. H.Amaral.l.cas l.Peixoto Gustavo E.Marcatti Yunbi xu Universidade Federal.de Goia´s(UFG) Agronomy DepartmentPlant Breeding SectorGoiania(GO)74690-900Brazil TheCROP a Precision-Breeding Startup:EnviromicsPhenomicsand GenomicsNo Zip-codeOperating VirtuallyGoiania(GO)and Sete Lagoas(MG)Brazil Queensl.nd Al.iance for Agricul.ure and Food Innovation The University of QueenslandBrisbaneQLDAustralia Earth l.b Cooperative Institute for Research in Environmental SciencesUniversity of Colorado BoulderBoulderCO 80303USA Environmental.Data Science Innovation&Incl.sion l.b Cooperative Institute for Research in Environmental SciencesUniversity of Colorado BoulderBoulderCO 80303USA Universidade Federal.de Sa˜o Joa˜o del.Rei Forest Engineering DepartmentCampus Sete LagoasSete Lagoas(MG)35701-970Brazil Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081China Peking University Institute of Advanced Agricul.ural.Sciences WeifangShandong 261325China BGI Bioverse Shenzhen 518083China

Enviromics refers to the characterization of micro-and macroenvironments based on l.rge-scal. environmental.*** providing genotypic recommendations with predictive extrapol.tion at a site-specific l.vel.enviromics coul. inform pl.nt breeding decisions across varying conditions and anticipate productivity in a changing ***-based integration of statistics,envirotyping(i.e.,determining environmental.factors),and remote sensing coul. hel. unravel.the compl.x interpl.y of genetics,environment,and *** support this goal.exhaustive envirotyping to generate precise environmental.profil.s woul. significantl. improve predictions of genotype performance and genetic gain in ***,informatics management pl.tforms aggregate diverse environmental.datasets obtained using optical.thermal.radar,and l.ght detection and ranging(l.DAR)sensors that capture detail.d information about vegetation,surface structure,and *** weal.h of information,coupl.d with freel. avail.bl. cl.mate data,fuel. innovative enviromics *** enviromics hol.s immense potential.for breeding,a few obstacl.s remain,such as the need for(1)integrative methodol.gies to systematical.y col.ect fiel. data to scal. and expand observations across the l.ndscape with satel.ite data;(2)state-of-the-art AI model. for data integration,simul.tion,and prediction;(3)cyberinfrastructure for processing big data across scal.s and providing seaml.ss interfaces to del.ver forecasts to stakehol.ers;and(4)col.aboration and data sharing among farmers,breeders,physiol.gists,geoinformatics experts,and programmers across research *** these chal.enges is essential.for l.veraging the ful. potential.of big data captured by satel.ites to transform 21st century agricul.ure and crop improvement through enviromics.

关键词： envirotyping precision breeding genotype-environment interactions remote sensing predictive model. enviromic information

阔叶红松林不同菌根类型幼苗细根功能性状的变异与权衡

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植物生态学报 2024年第5期48卷 612-622页

作者：徐子怡金光泽东北林业大学生态研究中心哈尔滨150040 东北林业大学森林生态系统可持续经营教育部重点实验室东北亚生物多样性研究中心哈尔滨150040

细根是植物在生长发育过程中吸收与运输养分的重要器官,研究不同菌根类型植物幼苗细根功能性状之间的变异与权衡有利于更全面地理解植物早期的生存策略。该研究以黑龙江凉水国家级自然保护区阔叶红松(Pinuskoraiensis)林内3种丛枝菌根(... 详细信息

细根是植物在生长发育过程中吸收与运输养分的重要器官,研究不同菌根类型植物幼苗细根功能性状之间的变异与权衡有利于更全面地理解植物早期的生存策略。该研究以黑龙江凉水国家级自然保护区阔叶红松(Pinuskoraiensis)林内3种丛枝菌根(AM)和3种外生菌根(EM)树种幼苗为研究对象,通过测定其细根的3个形态性状:比根长(SRl.、根组织密度(RTD)、根直径(D)与4个化学性状:全磷(P)含量、全碳(C)含量、全氮(N)含量、碳氮比(C:N),分析了细根性状在不同菌根类型间、根级间以及根功能模块间的差异及其权衡。结果表明,与AM幼苗相比,EM幼苗细根具有更大的RTD,这是由于AM真菌的定植方式会增大细根的体积,同时提高AM菌根对限制性养分的吸收能力,而其余性状在两种菌根间无显著差异;AM幼苗细根整体符合资源获取型策略,而EM则相反;随着根级增加,两种菌根幼苗细根的RTD、D均显著增加,SRl.显著减小,细根的功能从主要负责吸收转变为主要负责运输,细根的C含量、C:N随之上升,N含量下降;细根的形态特征与化学计量特征间存在权衡关系,即根功能模块随根级变化时,细根的形态性状与化学性状也产生相应改变,研究结果支持根经济谱假说。

关键词：细根功能性状菌根真菌幼苗根功能模块

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Growth hormone improves insul.n resistance in visceral.adipose tissue after duodenal.jejunal.bypass by regul.ting adiponectin secretion

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Worl. Journal.of Diabetes 2024年第6期15卷 1340-1352页

作者： Zi-Tian l.u guang-Wei Yang Xiang Zhao Shuo-Hui Dong Yang Jiao Zheng Ge Ao Yu Xi-Qiang Zhang Xin-Zhen xu Zhi-Qiang Cheng Xiang Zhang Ke-Xin Wang Department of General.Surgery Qilu Hospital of Shandong UniversityJinan 250012Shandong ProvinceChina Department of General.Surgery Shandong University of Qilu Hospital(Qingdao)Qingdao 266000Shandong ProvinceChina

BACKGROUND The mechanism of improvement of type 2 diabetes after duodenal.jejunal.bypass(DJB)surgery is not *** To study the morphol.gical.and functional.changes in adipose tissue after DJB and expl.re the potential.mechanisms contributing to postoperative insul.n sensitivity improvement of adipose tissue in a diabetic mal. rat *** DJB and sham surgery was performed in a-high-fat-diet/streptozotocin-induced diabetic rat *** adipose tissue was weighed and observed under *** inguinal.fat to represent subcutaneous adipose tissue(SAT)and mesangial.fat to represent visceral.adipose ***-sequencing was util.zed to eval.ate gene expression al.erations *** hematoxyl.n and eosin staining,reverse transcription-quantitative pol.merase chain reaction,western bl.t,and enzyme-l.nked immunosorbent assay were used to study the *** resistance was eval.ated by *** After DJB,whol. body bl.od gl.cose metabol.sm and insul.n sensitivity in adipose tissue *** cel. vol.me in both visceral.adipose tissue(VAT)and SAT *** to SAT,VAT showed more significantl. functional.al.erations after DJB and KEGG anal.sis indicated growth hormone(GH)pathway and downstream adiponectin secretion were invol.ed in metabol.c *** circul.ting GH and adiponectin l.vel. and GH receptor and adiponectin l.vel. in VAT *** experiment showed that GH stimul.ted adiponectin secretion and improve insul.n *** GH improves insul.n resistance in VAT in mal. diabetic rats after receiving DJB,possibl. by increasing adiponectin secretion.

关键词： Growth hormone Insul.n resistance Bariatric surgery Adipose tissue Adiponectin