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Alu antisense RNA ameliorates methylglyoxal-induced human lens epithelial cell apoptosis by enhancing antioxidant defense

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International Journal of Ophthalmology(English edition) 2023年第2期16卷 178-190页

作者： Pei-Yuan Wu Ning Ji Chong-guang Wu Xiao-Die Wang Xin liu Zhi-Xue Song Murad Khan Suleman Shah Ying-Hua Du Xiu-Fang Wang li-Fang Yan Department of Genetics Hebei Medical UniversityHebei Key Lab of Laboratory AnimalShijiazhuang 050017Hebei ProvinceChina Department of Ophthalmology Beijing Tiantan HospitalCapital Medical UniversityBeijing 100070China

AIM:To determine whether an antisense RNA corresponding to the human Alu transposable element(Aluas RNA)can protect human lens epithelial cells(HLECs)from methylglyoxal-induced ***:Cell counting kit-8(CCK-8)and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assays were used to assess HLEC *** viability/death was detected using a Calcein-AM/PI double staining kit;the annexin V-FITC method was used to detect HLEC *** cytosolic reactive oxygen species(ROS)levels in HLECs were determined using a reactive species assay *** levels of malondialdehyde(MDA)and the antioxidant activities of total-superoxide dismutase(T-SOD)and glutathione peroxidase(GSH-Px)were assessed in HLECs using their respective ***-q PCR and Western blotting were used to measure m RNA and protein expression levels of the ***:Aluas RNA rescued methylglyoxal-induced apoptosis in HLECs and ameliorated both the methylglyoxalinduced decrease in Bcl-2 m RNA and the methylglyoxalinduced increase in Bax m *** addition,Aluas RNA inhibited the methylglyoxal-induced increase in Alu sense RNA *** RNA inhibited the production of ROS induced by methylglyoxal,restored T-SOD and GSHPx activity,and moderated the increase in MDA content after treatment with *** RNA significantly restored the methylglyoxal-induced down-regulation of Nrf2 gene and antioxidant defense genes,including glutathione peroxidase,heme oxygenase 1,γ-glutamylcysteine synthetase and quinone oxidoreductase *** RNA ameliorated methylglyoxal-induced increases of the m RNA and protein expression of Keap1 that is the negative regulator of ***:Aluas RNA reduces apoptosis induced by methylglyoxal by enhancing antioxidant defense.

关键词： human Alu antisense RNA human lens epithelial cells methylglyoxal toxicity antioxidant defense apoptosis

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柴油微乳体系对生物油增溶性能研究

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中国环境科学 2015年第3期35卷 783-791页

作者：李闪星袁兴中冷立健王雪丽朱仁付丽欢李辉曾光明湖南大学环境科学与工程学院湖南长沙410082 湖南大学环境生物与控制教育部重点实验室湖南长沙410082

利用鼠李糖脂(RL)为表面活性剂,不同碳链长度正构醇为助表面活性剂构建柴油逆胶束体系,进而研究了该体系对模拟生物油的增溶性能.以单位柴油逆胶束增溶生物油的量为评价指标,研究了生物油和柴油的体积比B/D、醇的种类、醇与表面活性剂... 详细信息

利用鼠李糖脂(RL)为表面活性剂,不同碳链长度正构醇为助表面活性剂构建柴油逆胶束体系,进而研究了该体系对模拟生物油的增溶性能.以单位柴油逆胶束增溶生物油的量为评价指标,研究了生物油和柴油的体积比B/D、醇的种类、醇与表面活性剂的质量比C/R及表面活性剂的浓度对生物油增溶性能的影响,并对最佳增溶条件下获得的生物油/柴油微乳体系进行了性能分析,包括元素分析、傅里叶红外分析、热稳定性和燃料产品指标分析等.当生物油与柴油的体积比B/D为3:7,RL浓度为15g/L,助表面活性剂选用正庚醇且醇与表面活性剂的质量比C/R为2.0时,柴油微乳体系增溶生物油的量最大,性能较佳.

关键词：鼠李糖脂模拟生物油柴油微乳体系正构醇

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Management of Benign Tracheal Stenosis by Small-diameter Tube-assisted Bronchoscopic Balloon Dilatation

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Chinese Medical Journal 2015年第10期128卷 1326-1330页

作者： Yi-lin liang Guano-Nan liu Hou-Wen Zheng Yu li Le-Cheng Chen Yu-Yan Fu Wen-Tao li Si-ming Huang Mei-ling Yang Department of Respiratory Medicine First Affiliated Hospital of Guangxi Medical UniversityNanningGuangxi 530021China

Background：A limitation of bronchoscopic balloon dilatation （BBD） is that airflow must be completely blocked for as long as possible during the ***,the patient often cannot hold his or her breath for a long period affecting the efficacy of the *** this study,we used an extra-small-diameter tube to provide assisted ventilation to patients undergoing BBD and assessed the efficacy and safety of this ***：Bronchoscopic balloon dilatation was performed in 26 patients with benign tracheal stenosis using an extra-small-diameter *** tracheal diameter,dyspnea index,blood gas analysis results,and complications were evaluated before and after *** analyses were performed by SPSS version 16.0 for Windows （SPSS,Inc.,Chicago,IL,USA）.Results：Sixty-three BBD procedures were performed in 26 *** immediately improved in all patients after *** tracheal diameter significantly increased from 5.5 ± 1.5 mm to 13.0 ± 1.3 mm （P 〈 0.001）,and the dyspnea index significantly decreased from 3.4 ± 0.8 to 0.5 ± 0.6 （P 〈 0.001）.There was no significant change in the partial pressure of oxygen during the operation （before,102.5 ± 27.5 mmHg;during,96.9 ± 30.4 mmHg;and after,97.2 ± 21.5 mmHg;P =0.364）,but there was slight temporary retention of carbon dioxide during the operation （before,43.5 ± 4.2 mmHg;during,49.4 ± 6.8 mmHg;and after,40.1 ± 3.9 mmHg;P 〈 0.001）.Conclusion：Small-diameter tube-assisted BBD is an effective and safe method for the management of benign tracheal stenosis.

关键词： Balloon Dilatation Benign Tracheal Stenosis Bronchoscopy Tube

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Effects of grinding environment and lattice impurities on spodumene flotation

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Transactions of Nonferrous Metals Society of China 2019年第7期29卷 1527-1537页

作者： guang-li ZHU Yu-hua WANG Xu-ming WANG Jan D. MILLER Dong-fang LU Xia-yu ZHENG Yue-hao ZHAO Hai-tao ZHENG School of Minerals Processing and Bioengineering Central South UniversityChangsha 410083China College of Mines and Earth Sciences University of UtahSalt Lake City 84112USA

The effect of grinding on the spodumene flotation was investigated. The flotation response of spodumene ground by different mills was different, due to the variation of metal ions on spodumene surfaces caused by grinding environments and/or impurities. The samples were subjected to acid pickling treatment to remove most of the metal ions from the surfaces, and then all samples showed the same poor flotation response, which confirmed the significance of surface metal ions. Metal ion impurities may come from both grinding environments and lattice substitutions in spodumene. Density functional theory (DFT) calculation revealed that Fe and Ca could exist as lattice substitutions on the spodumene surface while Mg substitution is unlikely to occur. Furthermore, Fe is considered to be active site for the absorption of sodium oleate on the spodumene surface. Morphology analysis showed differences in particle size and shape for samples ground by different mills, resulting in different amounts of exposed surfaces. The particle size, cleavage characteristics caused by grinding environments, and metal ion impurities originated from grinding and isomorphous substitutions, play significant roles in the chemisorption of collector on the spodumene surface.

关键词： grinding lattice substitution metal ion impurities density functional theory (DFT) calculation spodumene cleavage flotation

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锰氧化物介导自养脱氮的启动、效能及机制

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中国给水排水 2024年第19期40卷 16-24页

作者：李光蕾李军沈阳建筑大学市政与环境工程学院辽宁沈阳110168

锰氧化物在厌氧条件下介导自养脱氮是一种很有前途的氨氮废水处理方法。通过调整pH、添加微量元素(EDTA和Fe^(2+))、采用低氨氮负荷的方式启动厌氧反应器,探究锰氧化物介导自养脱氮过程,分析微生物在脱氮过程中的作用。启动成功后,反应... 详细信息

锰氧化物在厌氧条件下介导自养脱氮是一种很有前途的氨氮废水处理方法。通过调整pH、添加微量元素(EDTA和Fe^(2+))、采用低氨氮负荷的方式启动厌氧反应器,探究锰氧化物介导自养脱氮过程,分析微生物在脱氮过程中的作用。启动成功后,反应器的氨氮去除负荷(ARR)和总氮去除负荷(NRR)分别为(0.031±0.009)、(0.025±0.010)kg(/m^(3)·d),且投加EDTA和Fe^(2+)促进了氨氮的去除。随着颗粒污泥的形成,锰氧化物逐渐由Mn(Ⅳ)向Mn(Ⅱ)转化,又经锰自养反硝化(NDMO)过程形成复杂的生物锰氧化物。反应器内存在多种脱氮菌群,包含锰氧化还原菌群Acinetobacter、Bacillus、Anaeromyxobacter和Geothrix,厌氧氨氧化菌Candidatus_Brocadia以及硝化-反硝化菌群Ellin6067、Nitrospira、norank_f__PHOS-HE36、Nitrosomonas和Denitratisoma。锰氧化物丰富了微生物的多样性,产生了多种脱氮途径。

关键词：锰氧化物自养脱氮启动微生物群落

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USP51/PD-L1/ITGB1-deployed juxtacrine interaction plays a cell-intrinsic role in promoting chemoresistant phenotypes in non-small cell lung cancer

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Cancer Communications 2023年第7期43卷 765-787页

作者： Jianjun li Xuechun Xiao Yang Ou lixia Cao Min Guo Chunchun Qi Zhaoyang Wang Yuxin liu Qiuying Shuai Hang Wang Peiqing Sun Yi Shi guang Yang Shuang Yang Tianjin Key Laboratory of Tumor Microenvironment and Neurovascular Regulation School of MedicineNankai UniversityTianjinP.R.China Department of Pulmonary and Critical Care Medicine The First Affiliated Hospital of Soochow UniversitySuzhouJiangsuP.R.China Department of Cancer Biology Wake Forest University School of MedicineWinston-SalemNCUSA College of Pharmacy Nankai UniversityTianjinP.R.China Institute of Transplantation Medicine Nankai UniversityTianjinP.R.China

Background:Programmed death ligand 1(PD-L1)has been demonstrated to facilitate tumor progression and therapeutic resistance in an immuneindependent ***,the function and underlying signaling network(s)of cancer cell-intrinsic PD-L1 action remain largely ***,we sought to better understand how ubiquitin-specific peptidase 51(USP51)/PD-L1/integrin beta-1(ITGB1)signaling performs a cell-intrinsic role in mediating chemotherapeutic resistance in non-small cell lung cancer(NSCLC).Methods:Western blotting and flow cytometry were employed for PD-L1 detection in NSCLC cell *** and pulldown analyses,protein deubiquitination assay,tissue microarray,bioinformatic analysis and molecular biology methods were then used to determine the significance of PD-L1 in NSCLC chemoresistance and associated signaling pathways in several different cell lines,mouse models and patient tissue ***-7-amido-4-methylcoumarin(Ub-AMC)-based deubiquitinase activity,cellular thermal shift and surface plasmon resonance(SPR)analyses were performed to investigate the activity of USP51 ***:We provided evidence that cancer cell-intrinsic PD-L1 conferred the development of chemoresistance by directly binding to its membrane-bound receptor ITGB1 in *** the molecular level,PD-L1/ITGB1 interaction subsequently activated the nuclear factor-kappa B(NF-κB)axis to elicit poor response to *** further determined USP51 as a bona fide deubiquitinase that targeted the deubiquitination and stabilization of the PD-L1 protein in chemoresistant NSCLC ***,we found a significant direct relationship between the USP51,PD-L1 and ITGB1 contents in NSCLC patients with chemoresistant *** elevated USP51,PD-L1 and ITGB1 levels were strongly associated with worse patient *** note,we identified that a flavonoid compound dihydromyricetin(DHM)acted as a potential USP51 inhibitor and rendered NSCLC cells more sensit

关键词： chemosensitivity dihydromyricetin immune-independence ITGB1 non-small cell lung cancer PD-L1 USP51

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心音的非线性时间序列分析

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传感技术学报 2005年第1期18卷 14-17,22页

作者：童基均汪亚明李光楼正国陈裕泉浙江大学生仪学院生物医学工程系杭州310027 浙江理工大学信息电子学院杭州310018

通过非线性时间序列分析的方法———相关维来分析心音时间序列 ,获得有关冠状动脉堵塞的血液动力学特征信息。对所有病例进行分析 ,发现通过相关维数刻划出了大多数冠状动脉堵塞病人的心舒期心音序列内在的确定性行为 ,而大多数正常人... 详细信息

通过非线性时间序列分析的方法———相关维来分析心音时间序列 ,获得有关冠状动脉堵塞的血液动力学特征信息。对所有病例进行分析 ,发现通过相关维数刻划出了大多数冠状动脉堵塞病人的心舒期心音序列内在的确定性行为 ,而大多数正常人的心舒期心音序列得不到具体的相关维数。这样提供了通过相关维数来对冠状动脉疾病进行无损检测的方法。

关键词：一t2,舒期相空间重构奇异值分解相关维

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Ginsenoside Rgl attenuates structural disruption of the blood-brain barrier to protect the central nervous system in ischemia/reperfusion

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中国天然药物 2013年第1期11卷 30-37页

作者： WANG Rui WANG guang-Ji WU Xiao-Lan ZHOU Fang li Yan-Nan liangsu Simcere Pharmaceutical R&D Co. Ltd. Nanjing 210042 China Key Laboratory of Drug Metabolism and Pharmacokinetics China Pharmaceutical University Nanjing 210009 China

目的:尽管人参皂甙Rg1有较强的神经保护作用,但前期研究表明Rg1难以透过血脑屏障到达脑实质。血脑屏障能维持中枢神经系统内环境的稳定,对中枢神经系统至关重要。本研究旨在验证Rg1是通过保护血脑屏障而发挥神经保护作用的观点。方法:雄... 详细信息

目的:尽管人参皂甙Rg1有较强的神经保护作用,但前期研究表明Rg1难以透过血脑屏障到达脑实质。血脑屏障能维持中枢神经系统内环境的稳定,对中枢神经系统至关重要。本研究旨在验证Rg1是通过保护血脑屏障而发挥神经保护作用的观点。方法:雄性SD大鼠才用大脑中动脉闭塞(MCAO)2h后再灌22h。Rg1(***1)于缺血后1h和再灌注3h通关尾静脉给药。动物模型测定血脑屏障的致密性,体外测定MDA和SOD。同时在体内外试验中测定基质金属蛋白酶的表达量和活性以及基质金属蛋白酶组织抑制剂mRNA的表达量以评估Rg1对血脑屏障结构的保护作用。结果:在缺血/再灌注模型大鼠中,依文思蓝的渗透率、基质金属蛋白酶的表达量和活性明显升高,而给以Rg1的模型鼠中这种升高得到明显抑制。缺血/再灌注模型大鼠中TIMP-2mRNA的表达降低,而给以Rg1的模型鼠中这种降低明显减轻。体外模型的结果与整体动物试验结果一致。结论:人参皂苷Rg1可能是通过保护脑微血管内皮细胞和减少病理条件下基质金属蛋白酶的表达量和活性、降低细胞外基质的水解,达到保护血脑屏障结构完整的作用,从而间接发挥了中枢神经系统保护作用。

关键词：人参皂苷Rg1 血脑屏障基质金属蛋白酶基质金属蛋白酶组织抑制剂细胞外基质

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Effect of mushroom root fermentation broth on the umami taste and nutrients of Flammulina velutipes

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Journal of Future Foods 2023年第1期3卷 67-74页

作者： Zijian Wang Xiujing Bao Rongrong Xia Zhenshan Hou Yunting li Yao Feng Yafei Wang Song Pan Heran Xu Zhuqing Huang guang Xin College of Food Science Shenyang Agricultural UniversityShenyang 110866China Shenyang Hengsheng Biotechnology Development Co. Ltd.Shenyang 110500China

As one of the most appealing edible mushrooms,the quality of Flammulina velutipes can be affected by the cultivation substrate,which has an impact on the mushroom umami taste and *** our study,the effect of mushroom root fermentation broth(MRFB)based substrate on umami taste and nutrients of *** were evaluated;four proportions of MRFB were conducted(CK 0%,E16.7%,E213.4%,E320.1%).Results indicated that MRFB was an effective nutrient supplement for mushroom cultivation substrate.E2 and E3 showed higher crude fiber,crude fat,and soluble *** to CK,the content of monosodium glutamate-like(MSG-like)amino acids,essential amino acids,total amino acids,and total 5′-nucleotides in E2 and E3 were *** equivalent umami concentration(EUC)values in E1,E2 and E3 were 1.70,2.43 and 1.56 times that of CK,*** umami,saltness and sweetness taste values were found in ***,it signified that better umami and richer nutrients were achieved by using substrate with proper volume of MRFB,especially,E2 with 13.4%of ***,better mushroom quality could be derived from the application of MRFB in *** was a favorable nutrient supplement for *** cultivation substrate.

关键词： Flammulina velutipes Fermentation broth Umami taste Nutrients Electronic tongue Mushroom byproducts

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基于显著小波子带的轮廓结构不规则性检测

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计算机工程 2011年第22期37卷 19-23页

作者：余光光马莉李庆奇杭州电子科技大学生命信息与仪器工程学院杭州310018

为解决皮肤肿瘤轮廓结构的不规则性表达和特征提取问题,提出一种在重构肿瘤轮廓结构分量上利用局部分形维(LFD)提取轮廓不规则特征的方法。在神经网络中实现肿瘤分类,使用小波分解和Hausdroff Distance确定肿瘤轮廓结构分量所处的频带(... 详细信息

为解决皮肤肿瘤轮廓结构的不规则性表达和特征提取问题,提出一种在重构肿瘤轮廓结构分量上利用局部分形维(LFD)提取轮廓不规则特征的方法。在神经网络中实现肿瘤分类,使用小波分解和Hausdroff Distance确定肿瘤轮廓结构分量所处的频带(显著小波子带),根据重构轮廓结构分量、LFD派生轮廓的不规则性特征对黑色素瘤进行分类。实验结果表明,该方法具有较高的分类准确率、敏感度和特异度。

关键词：显著小波子带结构不规则性局部分形维肿瘤轮廓小波分解轮廓重构

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