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Horticulture Research 2015年第1期2卷 183-192页

作者： guang-long wang Fei Xiong Feng Que Zhi-Sheng Xu Feng wang Ai-Sheng Xiong State Key Laboratory of Crop Genetics and Germplasm Enhancement College of HorticultureNanjing Agricultural UniversityNanjing 210095China Key Laboratories of Crop Genetics and Physiology of the Jiangsu Province and Plant Functional Genomics of the Ministry of Education Yangzhou UniversityYangzhou 225009China

Gibberellins(GAs)are considered potentially important regulators of cell elongation and expansion in *** undergoes significant alteration in organ size during its growth and ***,the molecular mechanisms underlying gibberellin accumulation and perception during carrot growth and development remain *** this study,five stages of carrot growth and development were investigated using morphological and anatomical structural *** levels in leaf,petiole,and taproot tissues were also investigated for all five *** levels in the roots initially increased and then decreased,but these levels were lower than those in the petioles and *** involved in gibberellin biosynthesis and signaling were identified from the carrotDB,and their expression was *** of the genes were evidently responsive to carrot growth and development,and some of them showed tissue-specific *** results suggested that gibberellin level may play a vital role in carrot elongation and *** relative transcription levels of gibberellin pathway-related genes may be the main cause of the different bioactive GAs levels,thus exerting influences on gibberellin perception and *** growth and development may be regulated by modification of the genes involved in gibberellin biosynthesis,catabolism,and perception.

关键词： insight roots initially

Ferromagnetism and magnetostructural coupling in V-doped MnNiGe alloys

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Chinese Physics B 2018年第10期27卷 565-569页

作者： Hui Yang Jun Liu Chao Li guang-long wang Yuan-Yuan Gong Feng Xu MIIT Key Laboratory of Advanced Metallic and Intermetallic Materials Technology School of Materials Science and EngineeringNanjing University of Science and TechnologyNanjing 210094China

The magnetostructural coupling between magnetic and structure transitions plays an important role in the multifunctional applications of magentocaloric materials. In this work, ferromagnetism and magnetostructural transformation are achieved in nonmagnetic V-doped MnNiGe alloys. With simultaneously reducing the transformation temperature and converting antiferromagnetic martensite to ferromagnetic state, the magnetostructural transformation between ferromagnetic orthorhombic phase and paramagnetic hexagonal phase is established in a temperature region as large as 130 K. The magnetic-field-induced magnetostructural transformation is accompanied by considerable magnetocaloric effect.

关键词： magnetostructural transformation ferromagnetism magnetocaloric effect

The genome sequence of celery(Apium graveolens L.),an important leaf vegetable crop rich in apigenin in the Apiaceae family

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Horticulture Research 2020年第1期7卷 2648-2657页

作者： Meng-Yao Li Kai Feng Xi-Lin Hou Qian Jiang Zhi-Sheng Xu guang-long wang Jie-Xia Liu Feng wang Ai-Sheng Xiong State Key Laboratory of Crop Genetics and Germplasm Enhancement Ministry of Agriculture and Rural Affairs Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East ChinaCollege of HorticultureNanjing Agricultural University1 WeigangNanjing 210095China

Celery(Apium graveolens L.)is a vegetable crop in the Apiaceae family that is widely cultivated and consumed because it contains necessary nutrients and multiple biologically active ingredients,such as apigenin and ***,we report the genome sequence of celery based on the use of HiSeq 2000 sequencing technology to obtain 600.8 Gb of data,achieving~189-fold genome coverage,from 68 sequencing libraries with different insert sizes ranging from 180 bp to 10 kb in *** assembled genome has a total sequence length of 2.21 Gb and consists of 34,277 predicted *** DNA sequences represent 68.88%of the genome sequences,and LTR retrotransposons are the main components of the repetitive *** analysis showed that a recent whole-genome duplication event may have occurred in celery,which could have contributed to its large genome *** genome sequence of celery allowed us to identify agronomically important genes involved in disease resistance,flavonoid biosynthesis,terpenoid metabolism,and other important cellular *** comparative analysis of apigenin biosynthesis genes among species might explain the high apigenin content of *** whole-genome sequences of celery have been deposited at CeleryDB(http://***/celerydb).The availability of the celery genome data advances our knowledge of the genetic evolution of celery and will contribute to further biological research and breeding in celery as well as other Apiaceae plants.

关键词： metabolism breeding sizes

Advances in research on the carrot,an important root vegetable in the Apiaceae family

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Horticulture Research 2019年第1期6卷 860-874页

作者： Feng Que Xi-Lin Hou guang-long wang Zhi-Sheng Xu Guo-Fei Tan Tong Li Ya-Hui wang Ahmed Khadr Ai-Sheng Xiong State Key Laboratory of Crop Genetics and Germplasm Enhancement Ministry of Agriculture and Rural AffairsKey Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East ChinaCollege of HorticultureNanjing Agricultural University1 Weigang210095 NanjingChina School of Life Science and Food Engineering Huaiyin Institute of Technology223003 HuaianChina Faculty of Agriculture Damanhour UniversityDamanhourEgypt

Carrots(Daucus carota L.),among the most important root vegetables in the Apiaceae family,are cultivated *** storage root is widely utilized due to its richness in carotenoids,anthocyanins,dietary fiber,vitamins and other *** extracts,which serve as sources of antioxidants,have important functions in preventing many *** biosynthesis,metabolism,and medicinal properties of carotenoids in carrots have been widely *** on hormone regulation in the growth and development of carrots has also been widely ***,with the development of high-throughput sequencing technology,many efficient tools have been adopted in carrot research.A large amount of sequence data has been produced and applied to improve carrot breeding.A genome editing system based on CRISPR/Cas9 was also constructed for carrot *** this review,we will briefly summarize the origins,genetic breeding,resistance breeding,genome editing,omics research,hormone regulation,and nutritional composition of *** about future research work on carrots are also briefly provided.

关键词： carrot breeding metabolism

Telomere-to-telomere carrot (Daucus carota) genome assembly reveals carotenoid characteristics

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Horticulture Research 2023年第7期10卷 112-124页

作者： Ya-Hui wang Pei-Zhuo Liu Hui Liu Rong-Rong Zhang Yi Liang Zhi-Sheng Xu Xiao-Jie Li Qing Luo Guo-Fei Tan guang-long wang Ai-Sheng Xiong State Key Laboratory of Crop Genetics&Germplasm Enhancement and Utilization Ministry of Agriculture and Rural Affairs Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East ChinaCollege of HorticultureNanjing Agricultural UniversityNanjingJiangsu 210095China Institute of Horticulture Guizhou Academy of Agricultural SciencesGuiyangGuizhou 550025China School of Life Science and Food Engineering Huaiyin Institute of TechnologyHuaianJiangsu 223003China Beijing Vegetable Research Center Beijing Academy of Agriculture and Forestry SciencesKey Laboratory of Biology and Genetic Improvement of Horticultural Crops in North ChinaBeijing 100097China

Carrot(Daucus carota)is an Apiaceae plant with multi-colored fleshy roots that provides a model system for carotenoid *** this study,we assembled a 430.40 Mb high-quality gapless genome to the telomere-to-telomere(T2T)level of“Kurodagosun”*** total,36268 genes were identified and 34961 of them were functionally *** proportion of repeat sequences in the genome was 55.3%,mainly long terminal *** on the coverage of the repeats,14 telomeres and 9 centromeric regions on the chromosomes were predicted.A phylogenetic analysis showed that carrots evolved early in the family *** on the T2T genome,we reconstructed the carotenoid metabolic pathway and identified the structural genes that regulate carotenoid *** the 65 genes that were screened,9 were newly ***,some gene sequences overlapped with transposons,suggesting replication and functional differentiation of carotenoid-related genes during carrot *** that some gene copies were barely expressed during development,they might be functionally *** of 24 cytochrome P450 genes associated with carotenoid biosynthesis revealed the tandem or proximal duplication resulting in expansion of CYP gene *** results provided molecular information for carrot carotenoid accumulation and contributed to a new genetic resource.

关键词： carrot sequences characteristics

AgMYB1,an R2R3-MYB factor,plays a role in anthocyanin production and enhancement of antioxidant capacity in celery

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Vegetable Research 2021年第1期1卷 2-13页

作者： Kai Feng Guo-Ming Xing Jie-Xia Liu Hao wang Guo-Fei Tan guang-long wang Zhi-Sheng Xu Ai-Sheng Xiong State Key Laboratory of Crop Genetics and Germplasm Enhancement Ministry of Agriculture and Rural Affairs Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East ChinaCollege of HorticultureNanjing Agricultural University1 WeigangNanjing 210095China Collaborative Innovation Center for Improving Quality and Increase Profits of Protected Vegetables in Shanxi Shanxi Agricultural UniversityTaigu 030801China Institute of Horticulture Guizhou Academy of Agricultural SciencesGuiyang 550006China School of Life Science and Food Engineering Huaiyin Institute of TechnologyHuaian 223001China College of Horticulture and Plant Protection Yangzhou UniversityYangzhou 225009China

Celery is rich in nutrients and cultivated *** are natural plant pigments with high antioxidant capabilities in the human *** accumulation of anthocyanins in celery results in the purple skin color of ***,an R2R3-MYB transcription factor(TFs),AgMYB1,was cloned from purple-skin *** analysis revealed that AgMYB1 belongs to the anthocyanin *** alignment showed that AgMYB1 contains multiple anthocyanin-related *** with the activating role in anthocyanin production,AgMYB1 showed higher transcriptions in purple celery compared with non-purple *** expression of AgMYB1 in tobacco leaves promoted the accumulation of anthocyanins and produced red pigments in *** expression of AgMYB1 in Arabidopsis activates anthocyanin production and generates dark-purple *** enhancement of anthocyanin biosynthetic genes transcripts and glycosylation capacities in transgenic Arabidopsis verified the activating roles of AgMYB1 at the gene and protein level,*** antioxidant capacity of transgenic Arabidopsis was also increased compared to wild type ***,yeast two-hybrid assay proved that AgMYB1 interacted with bHLH TFs to regulate anthocyanin *** results show that the overexpression of single R2R3-MYB gene,AgMYB1,without coexpression of other TFs,can improve anthocyanin production and antioxidant capacity in transgenic *** study presents new information for anthocyanin regulatory mechanisms in purple celery and provides a strategy for cultivating plants with high levels of anthocyanins.

关键词： anthocyanin purple branch

Multiparameter magnetic resonance imaging-based radiomics model for the prediction of rectal cancer metachronous liver metastasis

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World Journal of Gastrointestinal Oncology 2025年第1期17卷 62-72页

作者： Zhi-Da long Xiao Yu Zhi-Xiang Xing Rui wang Department of Hepatobiliary and Pancreaticosplenic Surgery Jingzhou Hospital Affiliated to Yangtze UniversityJingzhou 434100Hubei ProvinceChina

BACKGROUND The liver,as the main target organ for hematogenous metastasis of colorectal cancer,early and accurate prediction of liver metastasis is crucial for the diagnosis and treatment of ***,this study aims to investigate the application value of a combined machine learning(ML)based model based on the multiparameter magnetic resonance imaging for prediction of rectal metachronous liver metastasis(MLM).AIM To investigate the efficacy of radiomics based on multiparametric magnetic resonance imaging images of preoperative first diagnosed rectal cancer in predicting MLM from rectal *** We retrospectively analyzed 301 patients with rectal cancer confirmed by surgical pathology at Jingzhou Central Hospital from January 2017 to December *** participants were randomly assigned to the training or validation queue in a 7:3 *** first apply generalized linear regression model(GLRM)and random forest model(RFM)algorithm to construct an MLM prediction model in the training queue,and evaluate the discriminative power of the MLM prediction model using area under curve(AUC)and decision curve analysis(DCA).Then,the robustness and generalizability of the MLM prediction model were evaluated based on the internal validation set between the validation queue *** Among the 301 patients included in the study,16.28%were ultimately diagnosed with MLM through pathological *** analysis showed that carcinoembryonic antigen,and magnetic resonance imaging radiomics were independent predictors of ***,the GLRM prediction model was developed with a comprehensive nomogram to achieve satisfactory *** prediction performance of GLRM in the training and validation queue was 0.765[95%confidence interval(CI):0.710-0.820]and 0.767(95%CI:0.712-0.822),*** with GLRM,RFM achieved superior performance with AUC of 0.919(95%CI:0.868-0.970)and 0.901(95%CI:0.850-0.952)in the training and validation queue,respect

关键词： Rectal cancer Metachronous liver metastases Magnetic resonance imaging Radiomics Machine learning

MoS_(2)Lubricate‑Toughened MXene/ANF Composites for Multifunctional Electromagnetic Interference Shielding

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Nano-Micro Letters 2025年第2期17卷 358-371页

作者： Jiaen wang Wei Ming longfu Chen Tianliang Song Moxi Yele Hao Zhang long Yang Gegen Sarula Benliang Liang Luting Yan guangsheng wang School of Physical Science and Engineering Beijing Jiaotong UniversityBeijing 100044People’s Republic of China Key Laboratory of Bio‑Inspired Smart Interfacial Science and Technology of Ministry of Education School of ChemistryBeihang UniversityBeijing 100191People’s Republic of China

The design and fabrication of high toughness electromagnetic interference(EMI)shielding composite films with diminished reflection are an imperative task to solve electromagnetic pollution *** MXene/ANF(aramid nanofibers)–MoS_(2)composite films with nacre-like layered structure here are fabricated after the introduction of MoS_(2)into binary MXene/ANF composite *** introduction of MoS_(2)fulfills an impressive“kill three birds with one stone”improvement effect:lubrication toughening mechanical performance,reduction in secondary reflection pollution of electromagnetic wave,and improvement in the performance of photothermal *** the introduction of MoS_(2)into binary MXene/ANF(mass ratio of 50:50),the strain to failure and tensile strength increase from 22.1±1.7%and 105.7±6.4 MPa and to 25.8±0.7%and 167.3±9.1 MPa,*** toughness elevates from 13.0±4.1 to 26.3±0.8 MJ m^(−3)(~102.3%)*** the reflection shielding effectiveness(SE_(R))of MXene/ANF(mass ratio of 50:50)decreases~10.8%.EMI shielding effectiveness(EMI SE)elevates to 41.0 dB(8.2–12.4 GHz);After the introduction of MoS_(2)into binary MXene/ANF(mass ratio of 60:40),the strain to failure increases from 18.3±1.9%to 28.1±0.7%(~53.5%),the SE_(R)decreases~22.2%,and the corresponding EMI SE is 43.9 *** MoS_(2)also leads to a more efficient photothermal conversion performance(~45 to~55℃).Additionally,MXene/ANF–MoS_(2)composite films exhibit excellent electric heating performance,quick temperature elevation(15 s),excellent cycle stability(2,2.5,and 3 V),and long-term stability(2520 s).Combining with excellent mechanical performance with high MXene content,electric heating performance,and photothermal conversion performance,EMI shielding ternary MXene/ANF–MoS_(2)composite films could be applied in many industrial *** work broadens how to achieve a balance between mechanical properties and versatility of composites in the case of high-function fillers.

关键词： MXene-MoS_(2) Lubrication toughening EMI shielding Photothermal conversion Electric heating performance

Bowel preparation before colonoscopy: Consequences, mechanisms, and treatment of intestinal dysbiosis

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World Journal of Gastroenterology 2025年第2期31卷 13-26页

作者： Ze-long Zheng Qing-Fan Zheng Li-Qiang wang Yi Liu Department of Gastroenterology(Endoscopy Center) China-Japan Union Hospital of Jilin UniversityChangchun 130033Jilin ProvinceChina

The term“gut microbiota”primarily refers to the ecological community of various microorganisms in the gut,which constitutes the largest microbial community in the human *** adequate bowel preparation can improve the results of colonoscopy,it may interfere with the gut *** preparation for colonoscopy can lead to transient changes in the gut microbiota,potentially affecting an individual’s health,especially in vulnerable populations,such as patients with inflammatory bowel ***,measures such as oral pro-biotics may ameliorate these adverse *** focused on the bowel prepa-ration-induced changes in the gut microbiota and host health status,hypothesized the factors influencing these changes,and attempted to identify measures that may reduce dysbiosis,thereby providing more information for individualized bowel preparation for colonoscopy in the future.

关键词： Bowel preparation Colonoscopy Microbiota Inflammatory bowel disease Probiotics

Unraveling brain aging through the lens of oral microbiota

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Neural Regeneration Research 2025年第7期20卷 1930-1943页

作者： Qinchao Hu Si wang Weiqi Zhang Jing Qu guang-Hui Liu State Key Laboratory of Membrane Biology Institute of ZoologyChinese Academy of SciencesBeijingChina Hospital of Stomatology Guanghua School of StomatologyGuangdong Provincial Key Laboratory of StomatologySun Yat-sen UniversityGuangzhouGuangdong ProvinceChina Advanced Innovation Center for Human Brain Protection and National Clinical Research Center for Geriatric Disorders Xuanwu HospitalCapital Medical UniversityBeijingChina Aging Translational Medicine Center International Center for Aging and CancerBeijing Municipal Geriatric Medical Research CenterXuanwu HospitalCapital Medical UniversityBeijingChina CAS Key Laboratory of Genomic and Precision Medicine Beijing Institute of GenomicsChinese Academy of Sciences and China National Center for BioinformationBeijingChina University of Chinese Academy of Sciences BeijingChina Institute for Stem Cell and Regeneration Chinese Academy of SciencesBeijingChina Aging Biomarker Consortium BeijingChina State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijingChina Beijing Institute for Stem Cell and Regenerative Medicine BeijingChina

The oral cavity is a complex physiological community encompassing a wide range of *** of oral microbiota can lead to various oral infectious diseases,such as periodontitis and tooth decay,and even affect systemic health,including brain aging and neurodegenerative *** studies have highlighted how oral microbes might be involved in brain aging and neurodegeneration,indicating potential avenues for intervention *** this review,we summarize clinical evidence demonstrating a link between oral microbes/oral infectious diseases and brain aging/neurodegenerative diseases,and dissect potential mechanisms by which oral microbes contribute to brain aging and *** also highlight advances in therapeutic development grounded in the realm of oral microbes,with the goal of advancing brain health and promoting healthy aging.

关键词： Alzheimer's disease brain aging multiple sclerosis neurodegeneration neurodegenerative diseases oral microbiota Parkinson's disease periodontitis bacteria Porphyromonas gingivalis