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Expression and activity of inducible nitric oxide synthase and endothelial nitric oxide synthase correlate with ethanolinduced liver injury

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World Journal of Gastroenterology 2006年第15期12卷 2375-2381页

作者： guang-jin yuan xiao-rong zhou zuo-jiong gong pin zhang xiao-mei sun shi-hua zheng Department of Infectious Diseases Renmin Hospital of Wuhan UniversityWuhan 430060 Hubei Province China

AIM： To study the expression and activity of inducible nitric oxide synthase （iNOS） and endothelial nitric oxide synthase （eNOS） in rats with ethanol-induced liver injury and their relation with liver damage, activation of nuclear factor-KB （NF-κB） and tumor necrosis factor-α （TNF-α） expression in the liver. METHODS： Female Sprague-Dawley rats were given fish oil （0.5 mL） along with ethanol or isocaloric dextrose daily via gastrogavage for 4 or 6 wk. Liver injury was assessed using serum alanine aminotransferase （ALT） activity and pathological analysis. Liver malondialdehyde （MDA）, nitric oxide contents, iNOS and eNOS activity were determined. NF-κB p65, iNOS, eNOS and TNF-α protein or mRNA expression in the liver were detected by immunohistochemistry or reverse transcriptase-polymerase chain reaction （RT-PCR）. RESULTS： Chronic ethanol gavage for 4 wk caused steatosis, inflammation and necrosis in the liver, and elevated serum ALT activity. Prolonged ethanol administration （6 wk） enhanced the liver damage. These responses were accompanied with increased lipid peroxidation, NO contents, iNOS activity and reduced eNOS activity. NF-κB p65, iNOS and TNF-α protein or mRNA expression were markedly induced after chronic ethanol gavage, whereas eNOS mRNA expression remained unchanged. The enhanced iNOS activity and expression were positively correlated with the liver damage, especially the necro-inflammation, activation of NF-KB, and TNF-α mRNA expression. CONCLUSION： iNOS expression and activity are induced in the liver after chronic ethanol exposure in rats, which are correlated with the liver damage, especially the necro-inflammation, activation of NF-KB and TNF-α expression, eNOS activity is reduced, but its mRNA expression is not affected.

关键词： Alcoholic liver disease Inducible nitric oxide synthase Endothelial nitric oxide synthase Nuclear factor-κB

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绿茶多酚对实验性酒精性肝损伤大鼠的治疗作用

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世界华人消化杂志 2006年第1期14卷 50-56页

作者：周晓蓉龚作炯袁光金张频武汉大学人民医院感染科湖北省武汉市430060

目的:研究绿茶多酚及表没食子儿茶素没食子酸酯(EGCG)对大鼠酒精性肝损伤的治疗作用,并探讨其作用机制.方法:将48只大鼠随机分为正常对照组、模型组、茶多酚治疗Ⅰ组、Ⅱ组、EGCG治疗Ⅰ组、Ⅱ组,每组8只大鼠.以酒精+0.5mL鱼油灌胃制作... 详细信息

目的:研究绿茶多酚及表没食子儿茶素没食子酸酯(EGCG)对大鼠酒精性肝损伤的治疗作用,并探讨其作用机制.方法:将48只大鼠随机分为正常对照组、模型组、茶多酚治疗Ⅰ组、Ⅱ组、EGCG治疗Ⅰ组、Ⅱ组,每组8只大鼠.以酒精+0.5mL鱼油灌胃制作酒精性肝病模型.茶多酚治疗Ⅰ、Ⅱ组另外分别给予200mg/kg、100mg/kg茶多酚灌胃治疗,EGCGⅠ、Ⅱ治疗组则分别给予100mg/kg、50mg/kgEGCG灌胃治疗.5wk后处死大鼠,观察各组大鼠肝脏病理变化,并测定血清转氨酶及血浆内毒素水平,采用ELISA法检测血清TNF-α、IL-1、IL-18水平,并应用RT-PCR方法检测大鼠肝组织CD14、LBP、TNF-α、IL-1、IL-18mRNA的表达,并应用图像分析系统对其进行半定量分析.结果:模型组大鼠肝组织表现肝细胞中度脂肪变性,点状坏死,炎性细胞浸润,其血清ALT、TNF-α、IL-1、IL-18及血浆内毒素水平与正常对照组相比,显著升高(P<0.05或P<0.01);模型组肝组织CD14、LBP、TNF-α、IL-1、IL-18mRNA的表达与对照组相比显著增强(P<0.05或P<0.01).茶多酚、EGCG高低剂量分别同时处理显著降低了酒精性肝损伤大鼠血清ALT、TNF-α、IL-1、IL-18与血浆内毒素水平及肝组织CD14、LBP、TNF-α、IL-1、IL-18mRNA的表达(P<0.05或P<0.01),肝组织仍可见肝细胞脂肪变性,但未见坏死及炎性细胞浸润.结论:茶多酚及EGCG可减轻酒精性肝损伤大鼠肝脏的炎症与坏死,其可能机制包括降低内毒素血症,抑制Kupffer细胞活性与促炎细胞因子的表达与分泌.

关键词：酒精性肝病茶多酚 EGCG 内毒素血症细胞因子

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The first data release(DR1) of the LAMOST regular survey

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Research in Astronomy and Astrophysics 2015年第8期15卷 1095-1124页

作者： A-Li Luo Yong-Heng Zhao Gang Zhao Li-Cai Deng xiao-Wei Liu Yi-Peng jing Gang Wang Hao-Tong zhang Jian-rong shi Xiang-Qun Cui Yao-Quan Chu Guo-ping Li Zhong-Rui Bai Yue Wu Yan Cai Shu-Yun Cao Zi-huang Cao Jeffrey L.Carlin Hai-yuan Chen Jian-Jun Chen Kun-Xin Chen Li Chen Xue-Lei Chen xiao-Yan Chen Ying Chen Norbert Christlieb Jia-Ru Chu Chen-zhou Cui Yi-Qiao Dong Bing Du Dong-Wei Fan Lei Feng Jian-Ning Fu Peng Gao Xue-Fei gong Bo-Zhong Gu Yan-Xin Guo Zhan-Wen Han Bo-Liang He jin-Liang Hou Yong-Hui Hou Wen Hou Hong-Zhuan Hu Ning-Sheng Hu Zhong-Wen Hu Zhi-Ying Huo Lei Jia Fang-hua Jiang Xiang Jiang Zhi-Bo Jiang Ge jin xiao Kong Xu Kong Ya-Juan Lei Ai-hua Li Chang-hua Li guang-Wei Li Hai-Ning Li Jian Li Qi Li Shuang Li Sha-Sha Li Xin-Nan Li Yan Li Yin-Bi Li Ye-ping Li yuan Liang Chien-Cheng Lin Chao Liu Gen-rong Liu Guan-Qun Liu Zhi-Gang Liu Wen-Zhi Lu Yu Luo Yin-Dun Mao Heidi Newberg Ji-Jun Ni Zhao-Xiang Qi Yong-Jun Qi shi-Yin Shen Huo-Ming shi jing Song Yi-Han Song Ding-Qiang Su Hong-Jun Su zheng-Hong Tang Qing-Sheng Tao yuan Tian Dan Wang Da-Qi Wang Feng-Fei Wang Guo-Min Wang Hai Wang Hong-Chi Wang Jian Wang Jia-Ning Wang Jian-Ling Wang Jian-ping Wang Jun-Xian Wang Lei Wang Meng-Xin Wang Shou-Guan Wang Shu-Qing Wang Xia Wang Ya-Nan Wang You Wang Yue-Fei Wang You-Fen Wang Peng Wei Ming-Zhi Wei Hong Wu Ke-Fei Wu Xue-Bing Wu Yu-Zhong Wu xiao-zheng Xing Ling-Zhe Xu Xin-Qi Xu Yan Xu Tai-Sheng Yan De-hua Yang Hai-Feng Yang Hui-Qin Yang Ming Yang zheng-Qiu Yao Yong Yu Hui yuan Hai-Bo yuan Hai-Long yuan Wei-Min yuan Chao Zhai En-Peng zhang hua-Wei zhang Jian-Nan zhang Li-pin zhang Wei zhang Yong zhang Yan-Xia zhang zheng-Chao zhang Ming Zhao Fang zhou Xu zhou Jie Zhu Yong-Tian Zhu Si-Cheng Zou Fang zuo National Astronomical Observatories Chinese Academy of Sciences Department of Astronomy Peking University Shanghai Astronomical Observatory Chinese Academy of Sciences Nanjing Institute of Astronomical Optics & Technology National Astronomical ObservatoriesChinese Academy of Sciences University of Science and Technology of China Department of Physics Applied Physics and AstronomyRensselaer Polytechnic InstituteTroyNY 12180USA Department of Physics and Astronomy Earlham CollegeRichmondIN 47374USA Universitt Heidelberg Zentrum für AstronomieLandessternwarteKnigstuhl 1269117 HeidelbergGermany Department of Astronomy Beijing Normal University Yunnan Observatories Chinese Academy of Sciences Purple Mountain Observatory Chinese Academy of Sciences

The Large sky Area Multi-Object Fiber Spectroscopic Telescope（LAMOST） general survey is a spectroscopic survey that will eventually cover approximately half of the celestial sphere and collect 10 million spectra of stars, galaxies and QSOs. Objects in both the pilot survey and the first year regular survey are included in the LAMOST DR1. The pilot survey started in October 2011 and ended in June 2012, and the data have been released to the public as the LAMOST Pilot Data Release in August 2012. The regular survey started in September 2012, and completed its first year of operation in June 2013. The LAMOST DR1 includes a total of 1202 plates containing 2 955 336 spectra, of which 1 790 879 spectra have observed signalto-noise ratio（SNR） ≥ 10. All data with SNR ≥ 2 are formally released as LAMOST DR1 under the LAMOST data policy. This data release contains a total of 2 204 696 spectra, of which 1 944 329 are stellar spectra, 12 082 are galaxy spectra and 5017 are quasars. The DR1 not only includes spectra, but also three stellar catalogs with measured parameters： late A,FGK-type stars with high quality spectra（1 061 918 entries）, A-type stars（100 073 entries）, and M-type stars（121 522 entries）. This paper introduces the survey design, the observational and instrumental limitations, data reduction and analysis, and some caveats. A description of the FITS structure of spectral files and parameter catalogs is also provided.

关键词： techniques: spectroscopic survey—data release—catalog

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