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The Cloning and Sequencing of Read-through Protein Gene from BYDV-GAV Virus

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Agricultural Sciences in China 2001年第1期 45-49页

作者： CHANG Sheng-jun WANG Xi-feng LI Li MA Zhan-hong ZHOU guang-he State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Acaderny of Agricultural SciencesBeijing 100094

The cDNA of BYDV-GAV read-through protein(RTP)gene was amplified from the extracted RNA of BYDV-GAV by using the polymerase chain reaction(PCR),and cloned into pGEM-7zf(+).Its complete nucleotide sequence was determined by dideoxynucleotide chain-termination *** BYDV-GAV RTP gene consists of *** sequences were most similar to that of the RTP gene of BYDV-MAV with identities of 87.4%and 87.1%at the nucleotide and amino acid levels,respectively.

关键词： BYDV-GAV Read-through protein gene Sequence

Expression pattern and action analysis of genes associated with the responses to chemical stimuli during rat liver regeneration

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World Journal of Gastroenterology 2006年第45期12卷 7285-729 1页

作者： Shao-Wei Qin Li-feng Zhao Xiao-guang Chen Cun-Shuan Xu Key Laboratory for Cell Differentiation Regulation Xinxiang 453007 Henan Province China College of Life Science Henan Normal University Xinxiang 453007 Henan Province China

AIM: To study the genes associated with the responses to chemokines, nutrients, inorganic substances, organic substances and xenobiotics after rat partial hepatectomy (PH) at transcriptional level. METHODS: The associated genes involved in the five kinds of responses were obtained from database and literature, and the gene expression changes during liver regeneration in rats were checked by the Rat Genome 230 2.0 array. RESULTS: It was found that 60, 10, 9, 6, 26 genes respectively participating in the above five kinds of responses were associated with liver regeneration. The numbers of initially and totally expressed genes occurring in the initial phase of liver regeneration (0.5-4 h after PH), G0/G1 transition (4-6 h after PH), cell proliferation (6-66 h after PH), cell differentiation and structure- functional reconstruction (66-168 h after PH) were 51, 19, 52, 6 and 51, 43, 98, 68 respectively, illustrating that the associated genes were mainly triggered in the initiation and transition stages, and functioned at different phases. According to their expression similarity, these genes were classified into 5 groups: only up- regulated (47), predominantly up-regulated (18), only down-regulated (24), predominantly down-regulated (10), and up- and down-regulated (8). The total times of their up-regulated and down-regulated expression were 441 and 221, demonstrating that the number of up- regulated genes is more than that of the down-regulated genes. Their time relevance and gene expression patterns were classifi ed into 14 and 26 groups, showing that the cell physiological and biochemical activitieswere staggered, diversifi ed and complicated during liver regeneration in rats. CONCLUSION: The chemotaxis was enhanced mainly in the forepart and metaphase of LR. The response of regenerating liver to nutrients and chemical substances was increased, whereas that to xenobiotics was not strong. One hundred and seven genes associated with LR play important roles in the respon

关键词： Partial hepatectomy Rat Genome 230 2.0 array Responses to chemical substances Genes associated with liver regeneration

Preparation and characterization of wood/montmorillonite nanocomposites

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Forestry Studies in China 2006年第1期8卷 35-40页

作者： Lü Wen-hua Zhao guang-jie Xue Zheng-hua College of Material Science and Technology Beijing Forestry University Beijing 100083 P. R. China Research Institute of Wood Industry Chinese Academy of Forestry Beijing 100091 E R. China Faculty of Wood Science and Decoration Engineering Southwest Forestry College Kunming 650224 P. R. China

With montmorillonite （MMT） organically modified as organophilic MMT （OMMT） and water-soluble phenol formaldehyde resin （PF） as intermediate, Chinese fir （Cunningharnia lanceolata） wood/MMT nanocomposites （WMNC） were prepared via nano intercalation compounding and characterized by XRD, SEM and FTIR. Results show that： 1） the preparation of OMMT is very successful; 2） the self-made PF can effectively intercalate into MMT to increase markably its gallery distance and even exfoliate its nano silicate laminae; 3） the XRD analysis indicates that some exfoliated MMT enters the non-crystallized region of microfibrils in wood cell walls and the crystallinity degree of wood in WMNC decreases; 4） the SEM graphs show that multiform MMT exists in WMNC. Some grains block in wood cell lumen, some layers adhere to the wood surface of the inner cell wall and some exfoliated nanolaminae even insert into wood cell walls; and 5） the FTIR analysis suggests that MMT and wood in WMNC perhaps interact via certain chemical bonding.

关键词： Chinese fir (Cunninghamia lanceolata) montmorillonite (MMT) nano intercalation compounding preparation and characterization

Co-culture with microglia promotes neural stem cells differentiation into astrocytes

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Chinese Medical Journal 2011年第20期124卷 3394-3398页

作者： GU feng WANG Juan FU Li MA Yong-jie Department of Breast PathologyTianjin Medical University Cancer Institute and Hospital Key Laboratory of Breast Cancer Prevention and Therapy of the Ministry of Education and Key Laboratory of Cancer Prevention and Therapy of Tianjin Tianjin 300060 China Department of Oncology Tianjin Medical University Cancer Institute and Hospital Key Laboratory of Breast Cancer Prevention and Therapy of the Ministry of Education and Key Laboratory of Cancer Prevention and Therapy of Tianjin Tianjin 300060 China Department of Gynecology Tianjin Central Gynecology and Obstetrics Hosoital Tianiin 300052 China

Background Neural stem cells （NSCs） are a self-renewing and multipotent population of the central nervous system （CNS）, which are active during development and maintain homeostasis and tissue integrity throughout life. Microglias are an immune cell population resident in the CNS, which have crucial physiological functions in the developing and adult CNS. This study aimed to investigate that whether microglia co-cultured with NSCs could promote astrogliogenesis from NSCs. Methods Microglia and NSCs were co-cultured in 24-well insert plates. NSCs were plated in the bottom of the well and microglia in the insert. Fluorescent staining, Western blotting and RT-PCR were used to determine the effect of microglia on NSCs differentiation. Results Co-culture of microglia and NSCs promoted astrogliogenesis from NSCs. Several key genes, such as Notch 1, Notch 2, Notch 3, Hes 5, and NRSFwere downregulated, while the critical genes Idl and Id2 were upregulated. BMP2 and FGF2 were upregulated. Conclusion Microglias act as a regulator of NSCs astrogliogenesis.

关键词： microglia stem cells astrogliogenesis

Relationships among androgen receptor CAG repeat polymorphism, sex hormones and penile length in Han adult men from China： a cross-sectional study

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Asian Journal of Andrology 2014年第3期16卷 478-481页

作者： Yan-Min Ma Kai-jie Wu Liang Ning Jin Zeng Bo Kou Hong-Jun Xie Zhen-Kun Ma Xin-Yang Wang Yong-guang Gong Da-Lin He Department of Urology First Affiliated Hospital of Medical School Xi'an Jiaotong University Xi'an Oncology Research Lab Key Laboratory of Environment and Genes Related to Diseases Ministry of Education of the People's Republic of China Xi'an China.

This study aimed to investigate the correlations among androgen receptor （AR） CAG repeat polymorphism, sex hormones and penile length in healthy Chinese young adult men. Two hundred and fifty-three healthy men （aged 22.8 ± 3.1years） were enrolled. The individuals were grouped as CAG short （CAGs） if they harbored repeat length of 〈20 or as CAG long （CAGL） if their CAG repeat length was 〉20. Body height/weight, penile length and other parameters were examined and recorded by the specified physicians; CAG repeat polymorphism was determined by the polymerase chain reaction （PCR） method; and the serum levels of the sex hormones were detected by radioimmunoassay. Student＇s t-test or linear regression analysis was used to assess the associations among AR CAG repeat polymorphism, sex hormones and penile length. This investigation showed that the serum total testosterone （T） level was positively associated with the AR CAG repeat length （P = 0.01）; whereas, no significant correlation of T or AR CAG repeat polymorphism with the penile length was found （P = 0.593）. Interestingly, an inverse association was observed between serum prolactin （PRL） levels and penile length by linear regression analyses （β = -0.024, P = 0.039, 95% confidence interval （CI）： -0.047, 0）. Collectively, this study provides the first evidence that serum PRL, but not T or AR CAG repeat polymorphism, is correlated with penile length in the Han adult population from northwestern China.

关键词： androgen receptor CAG repeat testosterone prolactin penile length

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体素效率加权平均法求解核废物桶SGS效率

核电子学与探测技术 2024年第4期44卷 648-653页

作者：郑洪龙庹先国杨剑波李锐苟睿陈杰符永东冯昊筠李宇浩魏志恒四川轻化工大学自贡643000

采用了一种基于体素效率加权平均的核废物桶SGS效率数值计算方法,通过空间点源效率函数确定断层中体素的无衰减效率,计算体素发射的γ射线进入探测器过程中在桶内介质中的衰减长度,结合介质的线衰减系数确定体素的衰减效率,对断层中所... 详细信息

采用了一种基于体素效率加权平均的核废物桶SGS效率数值计算方法,通过空间点源效率函数确定断层中体素的无衰减效率,计算体素发射的γ射线进入探测器过程中在桶内介质中的衰减长度,结合介质的线衰减系数确定体素的衰减效率,对断层中所有体素的衰减效率进行加权平均,实现断层的衰减效率计算。结果表明:桶内^(137)Cs核素的活度重建偏差为-32.53%~19.38%,实验验证了该效率数值计算方法的有效性和准确性。

关键词：放射性废物分段 γ扫描效率计算线衰减系数

Single-Site Cu-Doped PdSn Wavy Nanowires for Highly Active,Stable,and CO-Tolerant Ethanol Oxidation Electrocatalysis

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Precision Chemistry 2023年第6期1卷 363-371页

作者： Jiaqi Su jie feng Yonggang feng Shangheng Liu Bingyan Xu Yue Lin Jinyu Ye Ying Zhang Youyong Li Qi Shao Xiaoqing Huang College of Chemistry Chemical Engineering and Materials ScienceSoochow UniversitySuzhou 215123China Institute of Functional Nano&Soft Materials(FUNSOM) Soochow UniversityJiangsu 215123China State Key Laboratory of Physical Chemistry of Solid Surfaces College of Chemistry and Chemical EngineeringXiamen UniversityXiamen 361005China Hefei National Laboratory for Physical Sciences at the Microscale University of Science and Technology of ChinaHefei 230026China Wenzhou Key Lab of Advanced Energy Storage and Conversion Zhejiang Province Key Lab of Leather EngineeringCollege of Chemistry and Materials EngineeringWenzhou UniversityWenzhou 325035China

Developing a catalyst to break the tradeoff relation-ship between the catalytic activity and antipoisoning property toward the ethanol oxidation reaction(EOR)is of critical importance to the development of direct ethanol fuel cells(DEFCs),but remains ***,we developed a unique class of single-site Cu-doped PdSn wavy nanowires(denoted as SS Cu−PdSn WNWs)with promoted activity and durability toward alkaline *** characterizations reveal the atomic isolation of Cu species dispersed on the surface of the PdSn WNWs with distinct wavy structure and grain *** created SS Cu−PdSn WNWs exhibit an enhanced EOR performance in terms of mass activity,which is higher than those of PdSn WNWs,commercial Pd black,and commercial Pd/C,***,the SS Cu−PdSn WNWs can also show improved stability as compared to other catalysts due to the improved antipoisoning property from the unique surface anchoring *** investigations demonstrate that the doped SS Cu can strongly inhibit the adsorption of CO and promote the reaction process of *** results reveal that the doped Cu shifts down the d-band center of PdSn,thereby modifying the adsorption of intermediates and reducing the reaction barrier of *** work maps a pathway for optimally boosting EOR performance with surface engineering via atomic doping.

关键词： single site PdSn nanowire ethanol oxidation reaction CO tolerant electrocatalysis

Discovery of human pancreatic lipase inhibitors from root of Rhodiola crenulata via integrating bioactivity-guided fractionation,chemical profiling and biochemical assay

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Journal of Pharmaceutical Analysis 2022年第4期12卷 683-691页

作者： Li-Juan Ma Xu-Dong Hou Xiao-Ya Qin Rong-Jing He Hao-Nan Yu Qing Hu Xiao-Qing Guan Shou-Ning Jia jie Hou Tao Lei guang-Bo Ge Shanghai Frontiers Science Center of TCM Chemical Biology Institute of Interdisciplinary Integrative Medicine ResearchShanghai University of Traditional Chinese MedicineShanghai201203China Department of Endocrinology Putuo HospitalShanghai University of Traditional Chinese MedicineShanghai200062China College of Basic Medical Sciences Dalian Medical UniversityDalian116044China Qinghai Hospital of Traditional Chinese Medicine Xining810099China

Although herbal medicines(HMs)are widely used in the prevention and treatment of obesity and obesity-associated disorders,the key constituents exhibiting anti-obesity activity and their molecular mechanisms are poorly ***,we assessed the inhibitory potentials of several HMs against human pancreatic lipase(hPL,a key therapeutic target for human obesity),among which the root-extract of Rhodiola crenulata(ERC)showed the most potent anti-hPL *** this study,we adopted an integrated strategy,involving bioactivity-guided fractionation techniques,chemical profiling,and biochemical assays,to identify the key anti-hPL constituents in *** ERC fractions(retention time=12.5e35 min),obtained using reverse-phase liquid chromatography,showed strong anti-hPL activity,while the major constituents in these bioactive fractions were subsequently identified using liquid chromatography-quadrupole time-of-flight mass spectrometry(LC-Q-TOF-MS/MS).Among the identified ERC constituents,1,2,3,4,6-penta-O-galloyl-β-D-glucopyranose(PGG)and catechin gallate(CG)showed the most potent anti-hPL activity,with pIC50 values of 7.59±0.03 and 7.68±0.23,*** investigations revealed that PGG and CG potently inhibited hPL in a non-competitive manner,with inhibition constant(Ki)values of 0.012 and 0.082 mM,***,our integrative analyses enabled us to efficiently identify and characterize the key anti-obesity constituents in ERC,as well as to elucidate their anti-hPL *** findings provide convincing evidence in support of the anti-obesity and lipid-lowering properties of ERC.

关键词： Human pancreatic lipase Rhodiola crenulata 1,2,3,4,6-Penta-O-Galloyl-β-D-glucopyranose Catechin gallate Inhibitory mechanism

Highly Stable CdSe/CdS/ZnS Fluorophores in Acidic Environment:Acile Preparation and Modification of Core/shell/shell Nanocrystals

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Chemical Research in Chinese Universities 2010年第6期26卷 871-875页

作者： YUE Yang GE Mei-ying LIU Yan WU jie CHEN Ping LIN Lie LIU Yu-feng SUN Yan CHEN Xin DAI Ning National Laboratory for Infrared Physics Shanghai Institute of Technical Physics Chinese Academy of Sciences Shanghai 200083 P R. China Key Laboratory of Opto-electronic Information Science and Technology Ministry of Education Institute of Modern Optics Nankai University Tianjin 300071 P. R. China

We described a facile method for preparing CdSe/CdS/ZnS core/shell/shell nanocrystals from air-stable single source *** single source precursors of cadmium ethylxanthate and zinc ethylxanthate were used to form CdS and ZnS shell layers in *** efficient modification of CdSe/CdS/ZnS nanocrystals was subsequently performed to obtain hydrophilic nanocrystal fluorophores with good stability in a pH range of 1.6—10.

关键词： Cadmium selenide Nanocrystal Photoluminescence Core/shell nanostructure