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Bioactivity of silk fibroin peptides on vascular endothelial cells

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Frontiers of Materials Science 2024年第1期18卷 85-94页

作者： Mengnan Dai Meng Li Peixuan Li Boyu zhang Jianmei Xu Jiannan Wang National Engineering Laboratory for Modern Silk College of Textile and Clothing EngineeringSoochow UniversityNo.199 Ren-ai RoadSuzhou Industrial ParkSuzhou 215123China

To determine the contribution of non-repetitive domains to the bioactivity of the heavy chain in silk fibroin(SF)macromolecules,a gene motif f(1)encoding this fragment and its multimers(f(4)and f(8))were biosynthesized from Escherichia coli *** on the positive application potential of SF materials for the vascular tissue engineering,this study focused on examining the active response of these polypeptides to vascular endothelial *** polypeptides F(1),F(4),and F(8)were separately grafted onto the surfaces of bioinert polyethylene terephthalate(PET)films,resulting in remarkable improvements in the spread and proliferation of human umbilical vein endothelial cells(HUVECs).Using the same grafting dose,the activity of cells on polypeptide-modified PET films enhanced with the increase of the molecular weight of those grafted polypeptides from F(1)to F(8).Meanwhile,the growth of cells on the surface of the alkaline-treated PET film was improved,indicating that the hydrophilicity of the surface material had influence on the growth of ***,on surfaces with the same water contact angle,the spread and proliferation activity of cells on PET films were significantly lower than those on polypeptide-modified PET films.

关键词： silk fibroin heavy chain non-repetitive fragment cytoactive endothelial cell

Low-coordination environment design of single Co atoms for efficient CO_(2) photoreduction

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Nano Research 2024年第5期17卷 3745-3751页

作者： Zhentao Ma Qingyu Wang Limin Liu Rong-Ao zhang Qichen Liu Peigen Liu Lihui Wu Chengyuan Liu Yu Bai Yida zhang Haibin Pan Xusheng Zheng National Synchrotron Radiation Laboratory University of Science and Technology of ChinaHefei 230029China College of Chemistry and Materials Science University of Science and Technology of ChinaHefei 230026China Experimental Center of Engineering and Material Science University of Science and Technology of ChinaHefei 230026China

Photocatalytic carbon dioxide(CO_(2))to carbon monoxide(CO)offers a promising way for both alleviating the greenhouse effect and meeting the industrial ***,we constructed a Co single-atom catalyst with intentional low-coordination environment design on porous ZnO(denoted as Co1/ZnO).Impressively,Co1/ZnO exhibited a remarkable activity with a CO yield rate of 22.25 mmol·g^(-1)·h^(-1) and a selectivity of 80.2%for CO_(2) photoreduction reactions under visible *** incorporation of single Co atoms provided an additional photo-generated electron transfer channel,which suppressed the carrier recombination of ***,the unsaturated Co active sites were capable to adsorb CO_(2) molecule spontaneously,thus facilitating the activation of CO_(2) molecule during CO_(2) reduction course.

关键词： photocatalytic CO_(2)reduction single atoms low-coordination CO_(2)adsorption electron transfer

Neosophoflavonoids A–C, A class of highly oxidized hybrid flavonoids from Sophora flavescens with antidiabetic effects

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Chinese Chemical Letters 2024年第10期35卷 321-324页

作者： Xu zhang Jiang Li Kai-Zhou Lu Ya-Nan Yang Jian-Shuang Jiang Xiang Yuan Zi-Ming Feng Fei Ye Pei-Cheng zhang State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College

Three highly oxidized hybrid flavonoids neosophoflavonoids A–C（1, 2a, and 2b） were isolated from the roots of Sophora flavescens. Neosophoflavonoid A possesses a unique highly oxidized heptacyclic6/6/6/6/6/6/5 system. Neosophoflavonoids B and C are isomers and share the same highly oxidized hexacyclic 6/6/6/6/6/6 systems. Their planar structures were elucidated from 1D/2D nuclear magnetic resonance（NMR）, ultraviolet spectroscopy（UV）, infrared spectroscopy（IR）, and high resolution electrospray ionization mass spectroscopy（HRESIMS） data. Their absolute configurations were determined by thorough GIAO13C NMR（DP4+） calculation protocol and electronic circular dichroism（ECD） calculation *** plausible biosynthetic routes for the compounds were also proposed. All compounds exhibited significant protein tyrosine phosphatase-1B（PTP1B） inhibitory activity with half maximal inhibitory concentration(IC50) values 3.94 ± 0.01, 0.38 ± 0.13, and 0.70 ± 0.01 μmol/L, respectively. In addition, compared to a positive control fenofibrate（Feno） at 20 μmol/L, compounds 2a and 2b exhibited stronger inhibitory effects on lipid accumulation in the oleic acid（OA）-induced cell model at 5 and 10 μmol/L.

关键词： Highly oxidized hybrid flavonoids Lipid accumulation inhibition Neosophoflavonoids A–C PTP1B inhibition Sophora flavescens

A centromere map based on super pan-genome highlights the structure and function of rice centromeres

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Journal of Integrative Plant Biology 2024年第2期66卷 196-207页

作者： Yang Lv Congcong Liu Xiaoxia Li Yueying Wang Huiying He Wenchuang He Wu Chen Longbo Yang Xiaofan Dai Xinglan Cao Xiaoman Yu Jiajia Liu Bin zhang Hua Wei Hong zhang Hongge Qian Chuanlin Shi Yue Leng Xiangpei Liu Mingliang Guo Xianmeng Wang Zhipeng zhang Tianyi Wang Bintao zhang Qiang Xu Yan Cui Qianqian zhang Qiaoling Yuan Noushin Jahan Jie Ma Xiaoming Zheng Yongfeng Zhou Qian Qian Longbiao Guo Lianguang Shang State Key Laboratory of Rice Biology China National Rice Research InstituteHangzhou 310006China Shenzhen Branch Guangdong Laboratory of Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120China Yazhouwan National Laboratory No.8 Huanjin RoadYazhou DistrictSanya City 572024China National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop ScienceChinese Academy of Agricultural SciencesBeijing 100081China

Rice(Oryza sativa)is a significant crop worldwide with a genome shaped by various evolutionary *** centromeres are crucial for chromosome segregation,and contain some unreported *** to the diverse and complex centromere region,a comprehensive understanding of rice centromere structure and function at the population level is *** constructed a high-quality centromere map based on the rice super pangenome consisting of a 251-accession panel comprising both cultivated and wild species of Asian and African *** showed that rice centromeres have diverse satellite repeat CentO,which vary across chromosomes and subpopulations,reflecting their distinct evolutionary *** also revealed that long terminal repeats(LTRs),especially young Gypsy-type LTRs,are abundant in the peripheral CentO-enriched regions and drive rice centromere expansion and ***,high-quality genome assembly and complete telomere-to-telomere(T2T)reference genome enable us to obtain more centromeric genome information despite mapping and cloning of centromere genes being *** investigated the association between structural variations and gene expression in the rice centromere.A centromere gene,OsMAB,which positively regulates rice tiller number,was further confirmed by expression quantitative trait loci,haplotype analysis and clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein9 *** revealing the new insights into the evolutionary patterns and biological roles of rice centromeres,our finding will facilitate future research on centromere biology and crop improvement.

关键词： centromere super pan-genome CentoO satellite repeat rice

Guidelines and consensus:Jejunoileostomy for diabetes mellitussurgical norms and expert consensus(2023 version)

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World Journal of Diabetes 2024年第11期15卷 2182-2188页

作者： Ji-Wei Shen Chun-Yong Ji Xue-Dong Fang Bo Yang Tian zhang Zheng-cai Li Hua-Zhi Li Zhi-Yi Liu Jun Tang Chuan-Wen Liao Ji-Zhou Lu Xuan Yang Xin-Guo zhang Department of General Surgery Heilongjiang Provincial People's HospitalHarbin 150036Heilongjiang ProvinceChina Department of General Surgery Zhengzhou Central HospitalZhengzhou 450007Henan ProvinceChina Department of General Surgery The Third Affiliated Hospital of Jilin UniversityChangchun 130033Jilin ProvinceChina Department of General Surgery The Eighth Medical Center of the General Hospital of the People's Liberation ArmyBeijing 100091China Department of General Surgery 242 Affiliated Hospital of Shenyang Medical CollegeShenyang 110034Liaoning ProvinceChina Department of General Surgery Jingmen Petrochemical General Hospital in Hubei ProvinceJingmen 448001Hubei ProvinceChina Department of General Surgery Beijing Chuiyangliu Hospital Affiliated to Tsinghua UniversityBeijing 100022China Department of General Surgery The Fourth Hospital of Jilin UniversityChangchun 130011Jilin ProvinceChina Department of General Surgery The First People's Hospital of XiangtanXiangtan 411101Hunan ProvinceChina Department of General Surgery Jiangxi First People's HospitalNanchang 332000Jiangxi ProvinceChina Department of General Surgery The Third People's Hospital of Gansu ProvinceLanzhou 730020Gansu ProvinceChina Department of General Thoracic Surgery Liaoning Electric Power Center HospitalShenyang 110403Liaoning ProvinceChina Department of General Surgery The Third Medical Center of the General Hospital of the People's Liberation ArmyBeijing 100039China

Diabetes mellitus(DM)is a group of diseases characterized by high blood glucose caused by insufficient absolute or relative secretion of *** diagnosed,patients need long-term treatment with hypoglycemic ***,the existing first-line hypoglycemic drugs do not provide effective treatment for DM and its *** the past,the first generation and the second generation of weight loss surgery,such as gastric bypass and sleeve gastric surgery,had strict body mass index ***,post-surgery,patients are prone to fluctuating hypoglycemia,gastroesophageal reflux,and dumping ***,the curative effect of this type of surgery was compromised to a certain *** is a third-generation surgery for patients with DM,which has been shown to improve glucose and lipid metabolism,without changing the original gastrointestinal tract *** from previous weight loss surgeries,jejunoileostomy has been clinically observed to delay the development of DM-related ***,the postoperative complications are mild and do not affect the patient’s quality of *** on our clinical observations from multi-center large samples,our team developed a consensus on the operative period and perioperative management of jejunoileostomy as a reference for clinical researchers.

关键词： Surgical Diabetes Weight loss surgery Y-shaped anastomotic jejunal loops Jejunoileostomy

不同应力养护条件对水泥土力学性能影响试验研究

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铁道科学与工程学报 2024年第2期21卷 637-647页

作者：陈昌富吕晨阳蔡焕张根宝湖南大学建筑安全与节能教育部重点实验室湖南长沙410082 湖南大学土木工程学院湖南长沙410082 湖南城市学院土木工程学院湖南益阳413000

为更好地了解水泥土在养护时期所受应力大小对其后期力学性能的影响,研发恒温等压或偏压条件下水泥土试样的养护装置和相应的试验方法。基于改制的恒温恒压水泥土试样养护装置,制作等压和偏压养护条件下不同龄期的水泥土试样。通过无侧... 详细信息

为更好地了解水泥土在养护时期所受应力大小对其后期力学性能的影响,研发恒温等压或偏压条件下水泥土试样的养护装置和相应的试验方法。基于改制的恒温恒压水泥土试样养护装置,制作等压和偏压养护条件下不同龄期的水泥土试样。通过无侧限压缩试验(UCT),获得等压和偏压养护条件下水泥土试样的应力-应变曲线及其无侧限抗压强度(UCS)和变形模量E_(50)随龄期的演化规律。试验结果表明:等压养护时围压σ_(c)从0.1 MPa增加到0.3 MPa,水泥土试样在龄期为7 d和14 d时的无侧限抗压强度和变形模量E_(50)均随着围压的增加而增大,但28 d龄期水泥土样的UCS和E_(50)随围压σ_(c)的增长而减小,甚至0.3 MPa围压养护试样的UCS已低于无围压养护试样的UCS,水泥土存在“高围压养护长期强度降低效应”;不同轴压比λ下偏压养护的水泥土样,其60 d龄期的UCS在偏压较小(即λλ_(c))时逐渐下降,即水泥土又存在“小偏压养护强度增加,大偏压养护强度降低的效应”。通过对试验结果进行回归分析,分别建立等压养护下计入围压参数σ_(c)的水泥土UCS和E_(50)的演化方程、偏压养护下水泥土UCS和E_(50)与轴压比λ的经验关系式。研究成果对于深刻理解环境条件对水泥土力学性能的影响机制和指导工程实践,具有较好的理论和工程实际意义。

关键词：水泥土无侧限抗压强度变形模量等压养护偏压养护

Germylene-Fe complexes caused by heterometallic coupling and investigation into the 3d-4p bonding

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Chinese Chemical Letters 2024年第3期35卷 304-307页

作者： Yingying Qin Yongmei Kang Lei zhang Jianping Sun Zhijing zhang Jialei Xu Fanlong Zeng Anyang Li Wenyuan Wang Weiqun Shi Key Laboratory of Synthetic and Natural Functional Molecule of Ministry of Education College of Chemistry and Materials ScienceNorthwest UniversityXi'an 710127China Laboratory of Nuclear Energy Chemistry Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China Shaanxi Coal and Chemical Industry Xi'an 710054China

The interpretation of heterometallic bonding nature is a basic work of inorganic *** means of intermetallic substitution of germylene anions with iron halide complexes CpFe(CO)_(2)I andβ-diketiminato Fe^Ⅱchloride,the ferrogermylene complexes 3a,3b and 4a were synthesized and structurally *** structural and IR characterizations show the presence of the Ge←Feπbackbonding in molecules 3a,3b and *** computational works on frontier molecular orbitals and their comparison of energy states confirmed thatσdonation andπbackbonding are both weak in these molecules,despite three complexes have longer Ge-Fe bonds,whose strength decreases slightly with the degressive electron density around Fe environment in a sequence from 3a,3b to 4a.

关键词： Metallic bonding Germylene Iron Crystal structure σbond πbackbond

非修饰靶点发现细胞热位移分析技术的变革与应用进展

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药学学报 2024年第1期59卷 25-34页

作者：刘广苑李亚慧张炜孔德志河北医科大学中西医结合研究所河北石家庄050017

了解药物的蛋白靶点研究方法对于新药研发、药物的临床应用、药物的作用机制和疾病的致病机制都具有十分重要的意义。细胞热位移分析(cellular thermal shift assay,CETSA)作为无修饰分析的靶点研究方法自开发以来就被广泛应用。经过不... 详细信息

了解药物的蛋白靶点研究方法对于新药研发、药物的临床应用、药物的作用机制和疾病的致病机制都具有十分重要的意义。细胞热位移分析(cellular thermal shift assay,CETSA)作为无修饰分析的靶点研究方法自开发以来就被广泛应用。经过不断地演化,现在已经出现了多种基于CETSA的技术结合,如基于质谱的细胞热位移分析(mass spectrometry-based cellular thermal shift assay,MS-CETSA)、结合等温剂量反应实验的细胞热位移分析(isothermal dose response-cellular thermal shift assay,ITDR-CETSA)、结合均相光激化学发光免疫分析的细胞热位移分析(amplified luminescent primity homogeneous assay-cellular thermal shift assay,Alpha-CETSA)等。这些技术融合了各自的优势,进一步扩展了CETSA的应用范围,适用于从药物筛选到监测药物在患者体内的靶点结合和脱靶毒性研究的整个药物开发链。本文结合作者的研究经验,综述了近年来CETSA和相关结合技术的原理及其在靶点发现上的应用、数据处理分析的进展,旨在为CETSA的进一步应用提供参考和借鉴。

关键词：细胞热位移分析热蛋白质组分析靶点发现技术结合

如何实现区域创新生态系统高水平双元创新?——基于战略三角的组态分析

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外国经济与管理 2024年第2期46卷 87-102页

作者：王寅贾翠雪张明明张建宇蔡双立天津财经大学国际工商学院天津300221 天津财经大学商学院天津300221

依据现有的系统创新分类,本文以2010—2019年中国31个省份区域创新生态系统的数据为样本,利用因子分析构建系统双元创新评价指标体系,基于战略三角理论构建“资源基础—共生网络—制度环境”前因条件组态,利用模糊集定性比较分析探究上... 详细信息

依据现有的系统创新分类,本文以2010—2019年中国31个省份区域创新生态系统的数据为样本,利用因子分析构建系统双元创新评价指标体系,基于战略三角理论构建“资源基础—共生网络—制度环境”前因条件组态,利用模糊集定性比较分析探究上述组态与区域创新生态系统高开发、高探索及高水平双元创新的复杂因果关系。研究发现:(1)共生网络作为核心条件包含于区域创新生态系统高开发性创新、高探索性创新及高水平双元创新全部组态。(2)数字化能力与共生网络形成显性基础设施与隐性网络关系的协同效应,促进区域创新生态系统高水平双元创新。(3)资源获取能力作为区域创新生态系统实现高水平双元创新的“边缘条件”,能提高创新主体搜索、获取、编排内外部创新资源的能力,缓解资源约束下自主创新不足等问题。(4)经济制度环境作为区域创新生态系统实现高水平双元创新的“边缘条件”,能为系统创新提供良好的运行框架与制度环境,并与其他前因条件形成良好组态效应。研究既完善了区域创新生态系统双元创新评价与判别体系,为后续研究提供量化基础;又拓宽了双元创新理论应用范畴,为系统治理者提供组态策略集与方法论。

关键词：区域创新生态系统双元创新资源基础共生网络制度环境