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Mutation of cytotoxin-associated gene A affects expressions of antioxidant proteins of Helicobacter pylori

World Journal of Gastroenterology 2009年第5期15卷 599-606页

作者： Zhi-Gang Huang guang-cai duan Qing-Tang Fan Wei-Dong Zhang Chun-Hua Song Xue-Yong Huang Rong-guang Zhang Department of Epidemiology College of Public HealthZhengzhou University Zhengzhou 450001 Henan ProvinceChina Department of Epidemiology School of Public Health Guangdong Medical College Dongguan 523808Guangdong Province China

AIM: To determine if disruption of the cagA gene of Helicobacter pylori (H pylori) has an effect on the expression of other proteins at proteome level. METHODS: Construction of a cagA knock out mutant Hp27 _△cagA (cagA -) via homologous recombination with the wild-type strain Hp27 (cagA+) as a recipient was performed. The method of sonication-urea-CHAPS-DTT was employed to extract bacterial proteins from both strains. Soluble proteins were analyzed by two-dimensional electrophoresis (2-DE). Images of 2-DE gels were digitalized and analyzed. Only spots that had a statistical signif icance in differential expression were selected and analyzed by matrix-assisted laser desorption/ionizationtime of flight mass spectrometry (MALDI-TOF-MS). Biological information was used to search protein database and identify the biological function of proteins. RESULTS: The proteome expressions between wild-type strain and isogenic mutant with the cagA gene knocked-out were compared. Five protein spots with high abundance in bacteria proteins of wild-type strains, down-regulated or absently expressed in bacteria proteins of mutants, were identified and analyzed. From a quantitative point of view, the identified proteins are related to the cagA gene and important antioxidant proteins of H pylori , including alkyl hydroperoxide reductase (Ahp), superoxide dismutase (SOD) and modulator of drug activity (Mda66), respectively, suggesting that cagA is important to maintain the normal activity of antioxidative stress and ensure H pylori persistent colonization in the host. CONCLUSION: cagA gene is relevant to the expressions of antioxidant proteins of H pylori, which may be a novel mechanism involved in H pylori cagA pathogenesis.

关键词： Helicobacter pylorr, Cytotoxin-associatedgene A Knock-out Antioxidant protein Two-dimensional electrophoresis

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Antithetical impacts of deleterious LRP1B mutations in non-squamous and squamous NSCLCs on predicting benefits from immune checkpoint inhibitor alone or with chemotherapy over chemotherapy alone: retrospective analyses of the POPLAR/OAK and CHOICE-01 trial

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Science China Life Sciences 2025年第1期68卷 249-262页

作者： Jinliang Wang Wenyong Zhou Yu Xu Jianchun duan Qiaoxia Zhou Guoqiang Wang Leo Li Chunwei Xu Wenxian Wang Shangli cai Zhijie Wang Jie Wang Department of Oncology The Fifth Medical Center of Chinese PLA General Hospital Department of Thoracic Surgery Shanghai Chest HospitalSchool of MedicineShanghai Jiao Tong University Burning Rock Biotech CAMS Key Laboratory of Translational Research on Lung Cancer State Key Laboratory of Molecular OncologyDepartment of Medical OncologyNational Cancer Center/National Clinical Research Center for ancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical College Institute of Basic Medicine and Cancer (IBMC) Chinese Academy of Sciences Department of Clinical Trial The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital)

In non-small cell lung cancers,the non-squamous and squamous subtypes（nsqNSCLC and sqNSCLC） exhibit disparities in pathophysiology,tumor immunology,and potential genomic correlates affecting responses to immune checkpoint inhibitor（ICI）-based *** our inhouse training cohort（n=85）,the presence of the LRP1B deleterious mutation（LRP1B-del） was associated with longer and shorter progression-free survival（PFS） on ICIs alone in nsqNSCLCs and sqNSCLCs,respectively(Pinteraction=0.008).These results were validated using a larger public ICI cohort(n=208,Pinteraction<0.001).Multiplex immunofluorescence staining revealed an association between LRP1B-del and increased and decreased numbers of tumor-infiltrating CD8+T cells in nsqNSCLCs（P=0.040） and sqNSCLCs（P=0.014）,*** the POPLAR/OAK cohort,nsqNSCLCs with LRP1B-del demonstrated improved PFS benefits from atezolizumab over docetaxel(hazard ratio（HR）=0.70,P=0.046),whereas this benefit was negligible in those without LRP1B-del（HR=1.05,P=0.64）.Conversely,sqNSCLCs without LRP1B-del benefited more from atezolizumab（HR=0.60,P=0.002） than those with LRP1B-del（HR=1.30,P=0.31）.Consistent results were observed in the in-house CHOICE-01 cohort,in which nsqNSCLCs with LRP1B-del and sqNSCLCs without LRP1B-del benefited more from toripalimab plus chemotherapy than from chemotherapy alone(Pinteraction=0.008).This multi-cohort study delineates the antithetical impacts of LRP1B-del in nsqNSCLCs and sqNSCLCs on predicting the benefits from ICI alone or with chemotherapy over chemotherapy *** findings highlight the distinct clinical utility of LRP1B-del in guiding treatment choices for nsqNSCLCs and sqNSCLCs,emphasizing the necessity for a detailed analysis based on pathological subtypes when investigating biomarkers for cancer therapeutics.

关键词： non-squamous NSCLC squamous NSCLC LRP1B deleterious mutations predictive biomarker immune checkpoint inhibitor

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Spin-modulated near-infrared-Ⅰ emission in a xanthene-decorated Fe(Ⅱ) spin-crossover complex

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Science China Chemistry 2025年

作者： Cheng Yi Duyong Chen Shihui Zhang Rui cai Chunying duan Yinshan Meng Tao Liu State Key Laboratory of Fine Chemicals Frontier Science Center for Smart Materials and Chemical Engineering School of Chemical Engineering Dalian University of Technology Instrumental Analysis Center Dalian University of Technology School of Chemistry Dalian University of Technology Liaoning Binhai Laboratory

Molecular materials showing synergetic coupling of near-infrared （NIR） fluorescence and spin-crossover （SCO） are promisingly applicable in in-vivo bioimaging,temperature sensing,and spintronic *** the related study faces formidable *** this work we graft the NIR fluorophore xanthene onto the dipyridyl substituted triazole ligand and construct a new Febased SCO material,{FeⅡ（ddxc-abpt）2[N（CN）2]2}(1,ddxc-abpt:（E）-4-(((3,5-di（pyridin-2-yl）-4H-1,2,4-triazol-4-yl)imino)methyl)-N,N-diethyl-2,3-dihydro-1H-xanthen-6-amine).Magnetic studies indicate that compound 1 exhibits thermally induced SCO property in both the solid and solution ***-temperature fluorescence emission spectra reveal a remarkable 7-fold increase in photoluminescence intensity upon the transition from the low-spin state at 110 K to the high-spin state at 280 K,which is the record of all reported coupled fluorescence-SCO ***-temperature ultraviolet-visible （UV-Vis）absorption spectra show a significant change in absorption intensity within the fluorescence emission window,consistent with the F?rster resonance energy transfer （FRET） ***-dependent density functional theory （TD-DFT） calculations demonstrate that the strong coupling is attributed to disrupted energy transfer between the xanthene fluorophore and high-spin *** work provides a feasible approach for expanding the synergetic SCO-fluorescence materials to the NIR-Iwavelength region,and hopefully contributes to the applications of tunable NIR-SCO molecule-based sensors and devices.

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Advances in genotypic antimicrobialresistance testing: a comprehensive review

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Science China Life Sciences 2025年第1期68卷 130-143页

作者： Boheng duan Xianjun Zeng Junping Peng Huan Kui College of Nanchang University Department of Imaging The Second Affiliated Hospital of Nanchang University NHC Key Laboratory of Systems Biology of Pathogens National Institute of Pathogen BiologyChinese Academy of Medical Sciences & Peking Union Medical College Key Laboratory of Respiratory Disease Pathogenomics Chinese Academy of Medical Sciences & Peking Union Medical College Key Laboratory of Pathogen Infection Prevention and Control (Ministry of Education) State Key Laboratory of Respiratory Health and MultimorbidityNational Institute of Pathogen BiologyChinese Academy of Medical Sciences & Peking Union Medical College

Antimicrobial resistance(AMR) represents a substantial threat to global public health, complicating the treatment of common infections and leading to prolonged illness and escalated healthcare expenses. To effectively combat AMR, timely and accurate detection is crucial for AMR surveillance and individual-based therapy. Phenotypic antibiotic resistance testing(AST) has long been considered the gold standard in clinical applications, serving as the foundation for clinical AMR diagnosis and optimized therapy. It has significantly contributed to ensuring patients′ health and the development of novel antimicrobials. Despite advancements in automated culture-based AST technologies,inherent limitations impede the widespread use of phenotypic AST in AMR surveillance. Genotypic AST technologies offer a promising alternative option, exhibiting advantages of rapidity, high sensitivity, and specificity. With the continuous advancement and expanding applications of genotypic AST technologies, such as microfluidics, mass spectrometry, and high-resolution melting curve analysis, new vigor has been injected into the development and clinical implementation of genotypic AST technologies. In this narrative review, we discuss the principles, applications, and advancements of emerging genotypic AST methods in clinical settings. The comprehensive review aims to highlight the significant scientific potential of emerging genotypic AST technologies in clinical AMR diagnosis, providing insights to enhance existing methods and explore novel approaches.

关键词： antimicrobial resistance (AMR) genotypic antibiotic resistance testing (AST) AST technologies review

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幽门螺杆菌中国临床分离株iceA基因突变株的构建和鉴定

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世界华人消化杂志 2009年第11期17卷 1098-1102页

作者：马一君段广才张荣光张卫东范清堂郑州大学公共卫生学院流行病学教研室河南省郑州市450001 河南中医学院基础医学院河南省郑州市450008 河南省分子医学重点学科开放实验室河南省郑州市450052

目的:构建幽门螺杆菌(Hpylori)iceA基因突变株.方法:克隆iceA基因及两侧的部分序列至载体pBluescriptSKⅡ(-)多克隆位点中.运用基因重组方法将卡那霉素抗性基因(kam)插人iceA基因片段之间,构建出带卡那霉素抗性标志的突变载体pBS-iceA-k... 详细信息

目的:构建幽门螺杆菌(Hpylori)iceA基因突变株.方法:克隆iceA基因及两侧的部分序列至载体pBluescriptSKⅡ(-)多克隆位点中.运用基因重组方法将卡那霉素抗性基因(kam)插人iceA基因片段之间,构建出带卡那霉素抗性标志的突变载体pBS-iceA-kam.再将突变载体转化至Hpylori中国临床分离株MEL-Hpylori27中,用卡那霉素筛选iceA基因的突变株,经PCR和DNA测序鉴定.结果:用PCR方法扩增突变株基因组DNA,结果显示卡那霉素抗性基因已插入iceA基因片段中,测序进一步证实已筛选获得Hpylori中国临床分离株iceA基因的突变株.结论:成功构建一株HpyloriiceA基因突变株.

关键词：幽门螺杆菌 iceA基因突变株

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cagA基因缺失的中国幽门螺杆菌突变菌株的构建及鉴定

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世界华人消化杂志 2006年第33期14卷 3190-3194页

作者：黄志刚段广才范清堂黄学勇郑州大学公共卫生学院流行病学教研室河南省郑州市450052 郑州大学河南省分子医学重点实验室河南省郑州市450052

目的:构建cagA基因缺失的中国幽门螺杆菌突变株.方法:运用基因同源重组方法将卡那霉素抗性基因(kmR)连接到PCR扩增cagA基因两端区域产生的2个基因片段之间,并共同插人到pBluescriptSKⅡ(-)载体的多克隆位点中,构建出带卡那霉素抗性标志... 详细信息

目的:构建cagA基因缺失的中国幽门螺杆菌突变株.方法:运用基因同源重组方法将卡那霉素抗性基因(kmR)连接到PCR扩增cagA基因两端区域产生的2个基因片段之间,并共同插人到pBluescriptSKⅡ(-)载体的多克隆位点中,构建出带卡那霉素抗性标志的缺失突变载体pBs-cagA-mutant.将突变载体转化到含完整cagA基因的突变受体MEL-Hpylori27菌株中.卡那霉素筛选出cagA基因缺失的中国幽门螺杆菌突变株,并经PCR和DNA测序鉴定.结果:构建的突变载体经限制性内切酶酶切分析显示,产生的条带与设计结果完全一致.PCR方法扩增突变株cagA,kmR基因显示,cagA基因已被完整敲除掉,DNA测序证实筛选得到了cagA基因缺失的幽门螺杆菌突变株.连续培养25代后证实,幽门螺杆菌突变株具有良好稳定性.结论:首次成功构建出1株中国人来源的、cagA基因缺失的幽门螺杆菌突变株.

关键词：幽门螺杆菌 cagA基因缺失表达突变体

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城乡儿童自我药疗现状及影响因素分析

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中国现代医学杂志 2017年第25期27卷 42-48页

作者：任静朝张光辉段广才陈璐周广远阚慧秦晨光范中原新乡医学院公共卫生学院河南省分子诊断与医学检验技术协同创新中心河南新乡453003

目的了解城乡儿童自我药疗现状及存在问题,并分析影响安全自我药疗的因素。方法利用调查问卷调查新乡市及其所辖县城、乡村18岁以上的女性,通过拦截方式获得调查对象。利用Epidata 3.0软件录入数据,运用SAS 9.2软件对数据进行统计学分析... 详细信息

目的了解城乡儿童自我药疗现状及存在问题,并分析影响安全自我药疗的因素。方法利用调查问卷调查新乡市及其所辖县城、乡村18岁以上的女性,通过拦截方式获得调查对象。利用Epidata 3.0软件录入数据,运用SAS 9.2软件对数据进行统计学分析。3地区自我药疗的相关情况利用频数、构成比等进行统计描述,3地区特征分布的比较利用χ2检验,并利用多因素Logistic回归分析影响认知得分高低的因素。结果收回家庭中有儿童者的有效问卷共1 045份。84.90%市区对象、88.00%县城对象和88.65%乡村对象曾给孩子自我药疗。给孩子自我药疗的最主要原因是病情较轻,3地区分别占53.27%、57.40%和60.16%,用药的主要依据是自身经验,3地区分别为56.25%、54.21%和40.65%。给儿童服药前,不经常阅读说明书的比例较高(市区:29.12%,县城:37.13%,乡村:39.34%),且3地区间差异无统计学意义(χ2=8.0625,P=0.089),3地区在是否按时给孩子用药、忘记给孩子用药时的处理等其他给儿童服药行为方面差异有统计学意义(P<0.05)。城乡、受教育程度、职业、身体状况、家庭月收入与自我药疗行为认知得分有关。结论城乡儿童自我药疗率均较高,儿童安全自我药疗行为和认知与城乡、受教育程度、职业、身体状况、家庭月收入有关,政府应针对不同地区、不同人群进行相关健康教育。

关键词：儿童自我药疗现况调查影响因素

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志贺菌基因转移耐多药相关蛋白初步分析

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世界华人消化杂志 2007年第8期15卷 838-843页

作者：宋春花黄志刚郗园林张梅喜段广才郑州大学公共卫生学院流行病学教研室河南省郑州市450001 河北工程大学医学院河北省邯郸市056002

目的:对志贺菌敏感株与基因转移耐多药株全菌蛋白进行蛋白质组学比较,寻找细菌耐多药相关蛋白．方法:采用接合基因转移实验对临床分离鉴定志贺菌敏感株进行基因转移耐多药试验,并对志贺菌敏感株及基因转移耐多药株全菌蛋白进行双向电泳... 详细信息

目的:对志贺菌敏感株与基因转移耐多药株全菌蛋白进行蛋白质组学比较,寻找细菌耐多药相关蛋白．方法:采用接合基因转移实验对临床分离鉴定志贺菌敏感株进行基因转移耐多药试验,并对志贺菌敏感株及基因转移耐多药株全菌蛋白进行双向电泳:电泳图谱采用Image Master 2D Platinum软件分析,并对差异表达蛋白进行MOLDI TOF-TOF质谱分析．结果:成功获得志贺菌基因转移耐多药株,在志贺菌敏感株与耐多药株全菌蛋白质图谱中分别检测出946±37个和1013±157个蛋白质斑点;经分析共发现43个差异表达的蛋白点,初步对其中5个表达量增加的差异蛋白进行质谱鉴定,基因转移耐多药株中发现两个新出现的耐多药相关蛋白分别为CRISPR相关蛋白及Hsp60的分子伴侣蛋白(Groel-Groes-Adp7); ABC转运蛋白、胱氨酸合成酶、预测的胞浆脂蛋白表达量上调．结论:通过对鉴定的5个耐多药相关蛋白分析初步发现供体菌中一些耐多药相关基因通过基因转移方式插入志贺菌敏感株中并大量表达,同时一些在细胞代谢中起重要作用的酶类表达量上调,ABC转运蛋白在志贺菌基因转移耐多药机制中也起重要作用．

关键词：志贺菌耐多药蛋白质组学双向电泳质谱分析差异表达

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Preparation and applications of calcium ferrite as a functional material:A review

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 292-310页

作者： Xiuli Han Bowen duan Lei Liu Shilong Fang Weiwei Wang College of Mining Engineering North China University of Science and TechnologyTangshan 063210China Collaborative Innovation Center of Green Development and Ecological Restoration of Mineral Resources of Hebei Province Tangshan 063210China Key Laboratory of Mining Development and Security Technology of Hebei Province Tangshan 063210China

Calcium ferrite(CF)is recognized as a potential green and efficient functional material because of its advantages of magnetism,electrochemistry,catalysis,and biocompatibility in the fields of materials chemistry,environmental engineering,and ***-fore,the obtained research results need to be systematically summarized,and new perspectives on CF and its composite materials need to be *** on the presented studies of CF and its composite materials,the types and structures of the crystal are *** addition,the current application technologies and theoretical mechanisms with various properties in different fields are ***,the various preparation methods of CF and its composite materials are elaborated in *** importantly,the advantages and disadvantages of the synthesis methods of CF and its composite materials are discussed,and the existing problems and emerging challenges in practical production are ***,the key future research directions of CF and its composite materials have been prospected from the potential application technologies to provide references for its synthesis and efficient utilization.

关键词： calcium ferrite mineral functional materials preparation application perspectives

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Distribution and mapping of temperate savanna in the sandy lands of eastern China

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Science China Earth Sciences 2025年第1期68卷 128-141页

作者： Xiaoya LI Feng WANG Tao duan Kaijie YANG Bin YANG Chunmei WANG Xin TIAN Qi LU Institute of Ecological Conservation and Restoration Institute of Desertification Studies Chinese Academy of Forestry Institute of Desertification Institute of Desertification Studies Chinese Academy of Forestry Institute of Great Green Wall Satellite Application Centre for Ecology and Environment MEE Institute of Microelectronics Chinese Academy of Sciences Aerospace Information Research Institute Chinese Academy of Sciences Institute of Forest Resource Information Techniques Chinese Academy of Forestry

Savannas are distributed globally across various *** perform a significant socioeconomic role in regulating net primary productivity and the terrestrial carbon *** to the high spatial heterogeneity induced by the mixed distribution of trees and grasses,current global land-cover classification maps often classify savannas as forests or grasslands Temperate savannas in China cover a vast area,yet their spatial patterns and extent remain poorly *** study developed a novel remote sensing temperate savannas mapping method by integrating very high-resolution （VHR） unmanned aerial vehicle （UAV） and GF-6 satellite imagery based on the vegetation structure characteristics of temperate savannas using the FAO definition of *** and validation datasets were collected from VHR UAV imagery covering the main temperate savannah types with a ground area of 1818 *** fractional woody vegetation cover （FWVC） and fractional herbaceous vegetation cover （FHVC） in temperate savannas were estimated by integrating Classification and Regression Tree and Random Forest *** current distribution of temperate savannas in the Horqin and Otindag Sandy Lands of Eastern China was mapped to a unit area of 0.5 *** root-mean-square error （RMSE） values for the FWVC and FHVC models were 10.69% and 11.67%,and the R2values were 0.66 and 0.63,*** assessment indicated that the new temperate savanna map had high identification accuracy,with a Kappa coefficient of 0.70,overall accuracy of 0.88,and average accuracy of *** savannas in the sandy lands of eastern China were primarily distributed in the northern Horqin Sandy Land the central and eastern regions of the Otindag Sandy Land,and the transition zone from the southern slopes of the Greater Khingan Mountains to the plains,covering a total area of 3.27×10～6 *** main savannah types were elm,oak/maple,and shrub *** savannas were widely distributed in the Otindag Sandy

关键词： Temperate savanna UAV imagery GF-6 Fractional woody vegetation cover Fractional herbaceous vegetation cover Machine learning

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