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Logic-gated tumor-microenvironment nanoamplifier enables targeted delivery of CRISPR/Cas9 for multimodal cancer therapy

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Acta Pharmaceutica Sinica B 2024年第2期14卷 795-807页

作者： Yongchun Pan Xiaowei luan Fei Zeng Xuyuan Wang Shurong Qin Qianglan Lu Guanzhong He Yanfeng Gao Xiaolian Sun Xin Han Bangshun He Yujun Song Department of Laboratory Medicine Nanjing First HospitalNanjing Medical UniversityNanjing 210006China College of Engineering and Applied Sciences State Key Laboratory of Analytical Chemistry for Life ScienceNanjing UniversityNanjing 210023China State Key Laboratory of Natural Medicines Key Laboratory of Drug Quality Control and PharmacovigilanceDepartment of Pharmaceutical AnalysisChina Pharmaceutical UniversityNanjing 211198China School of Medicine&Holistic Integrative Medicine Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources IndustrializationNanjing University of Chinese MedicineNanjing 210023China

Recent innovations in nanomaterials inspire abundant novel tumor-targeting CRISPR-based gene ***,the therapeutic efficiency of traditional targeted nanotherapeutic strategies is limited by that the biomarkers vary in a spatiotemporal-dependent manner with tumor ***,we propose a self-amplifying logic-gated gene editing strategy for gene/H_(2)O_(2)-mediated/starvation multimodal cancer *** this approach,a hypoxia-degradable covalent-organic framework(COF) is synthesized to coat a-ZIF-8 in which glucose oxidase(GOx) and CRISPR system are *** intensify intracellular redox dyshomeostasis,DNAzymes which can cleave catalase mRNA are loaded as *** the nano system gets into the tumor,the weakly acidic and hypoxic microenvironment degrades the ZIF-8@COF to activate GOx,which amplifies intracellular H^(+)and hypoxia,accelerating the nanocarrier degradation to guarantee available CRISPR plasmid and GOx release in target *** tandem reactions deplete glucose and oxygen,leading to logic-gated-triggered gene editing as well as synergistic gene/H_(2)O_(2)-mediated/starvation ***,this approach highlights the biocomputing-based CRISPR delivery and underscores the great potential of precise cancer therapy.

关键词： CRISPRdelivery Enzymeencapsulation Logic gate Hybridmaterial Hypoxia Gene editing Precise nanomedicine Multimodal therapy

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大数据能力对供应链韧性的影响--供应链整合的中介作用

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现代工业经济和信息化 2024年第5期14卷 184-189页

作者：袁峦中南民族大学管理学院湖北武汉430074

探讨了大数据能力对供应链韧性的影响机制,并同时分析供应链在这一过程中的中介作用。研究结果表明:企业的大数据能力对于供应链的整合与供应链韧性都发挥显著的正向影响;供应链整合在大数据能力影响供应链韧性的过程中发挥部分中介作... 详细信息

探讨了大数据能力对供应链韧性的影响机制,并同时分析供应链在这一过程中的中介作用。研究结果表明:企业的大数据能力对于供应链的整合与供应链韧性都发挥显著的正向影响;供应链整合在大数据能力影响供应链韧性的过程中发挥部分中介作用。研究结果对于探究大数据能力对供应链韧性的影响机制具有的理论启示意义,同时对数字化转型背景下推动企业高质量发展具有现实指导价值。

关键词：大数据能力供应链整合供应链韧性

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Synergistic anionic/zwitterionic mixed surfactant system with high emulsification efficiency for enhanced oil recovery in low permeability reservoirs

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Petroleum Science 2024年第2期21卷 936-950页

作者： Hai-Rong Wu Rong Tan Shi-Ping Hong Qiong Zhou Bang-Yu Liu Jia-Wei Chang Tian-Fang luan Ning Kang Ji-Rui Hou National Key Laboratory of Petroleum Resources and Engineering China University of Petroleum-BeijingBeijing102249China Unconventional Petroleum Research Institute China University of Petroleum-BeijingBeijing102249China Gas Production Plant 5 of PetroChina Changqing Oilfield Company PetroChinaXi'an710000ShaanxiChina College of Petroleum Engineering China University of Petroleum-BeijingBeijing102249China College of Science China University of Petroleum-BeijingBeijing102249China Kazakh-British Technical University Almaty050000Kazakhstan

Emulsification is one of the important mechanisms of surfactant flooding. To improve oil recovery for low permeability reservoirs, a highly efficient emulsification oil flooding system consisting of anionic surfactant sodium alkyl glucosyl hydroxypropyl sulfonate(APGSHS) and zwitterionic surfactant octadecyl betaine(BS-18) is proposed. The performance of APGSHS/BS-18 mixed surfactant system was evaluated in terms of interfacial tension, emulsification capability, emulsion size and distribution, wettability alteration, temperature-resistance and salt-resistance. The emulsification speed was used to evaluate the emulsification ability of surfactant systems, and the results show that mixed surfactant systems can completely emulsify the crude oil into emulsions droplets even under low energy conditions. Meanwhile,the system exhibits good temperature and salt resistance. Finally, the best oil recovery of 25.45% is achieved for low permeability core by the mixed surfactant system with a total concentration of 0.3 wt%while the molar ratio of APGSHS:BS-18 is 4:6. The current study indicates that the anionic/zwitterionic mixed surfactant system can improve the oil flooding efficiency and is potential candidate for application in low permeability reservoirs.

关键词： Anionic/zwitterionic mixed surfactant system Emulsification Synergistic effect Low permeability reservoir Enhanced oil recovery

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Natural variation in maize gene ZmSBR1 confers seedling resistance to Fusarium verticillioides

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The Crop Journal 2024年第3期12卷 836-844页

作者： Yunxia Song Peipei Ma Jingyang Gao Chaopei Dong Zhao Wang Yifan luan Jiafa Chen Doudou Sun Pei Jing Xuecai Zhang Weibin Song Zijian Zhou Jianyu Wu College of Agronomy Henan Agricultural UniversityZhengzhou 450002HenanChina College of Life Sciences Henan Agricultural UniversityZhengzhou 450002HenanChina Global Maize Program International Maize and Wheat Improvement Center(CIMMYT)Apdo 6-64106600 MexicoDFMexico National Maize Improvement Center Department of Plant Genetics and BreedingChina Agricultural UniversityBeijing 100193China

Maize seedling blight caused by Fusarium verticillioides is a widely occurring maize disease,but the genetics and mechanisms of resistance are not well *** this study,GWAS performed by MLM and 3VmrMLM identified 40 and 20 QTNs,associated with seedling blight *** methods identified 49 and 36 genes,*** verification of candidate gene ZmSBR1 identified by both methods showed that the resistance of a mutant line to seedling blight decreased by 0.37 grade points after inoculation with ***,compared with the *** length of the stem rot lesion caused by *** increased by 86%in mutant seedlings,and the relative length of the adult plant stalk rot increased by 35%in mutant plants compared to the wild type after inoculation with Fusarium *** analysis showed that expression of defense-related genes after inoculation was down-regulated in the mutant compared to the wild type,synthesis of secondary metabolites associated with resistance was reduced,and the immune response triggered by PAMP decreased,resulting in decreased resistance of mutant maize *** gene association analysis showed that most maize inbred lines carried the susceptible haplotype.A functional PCR marker was *** results demonstrated that ZmSBR1 conferred resistance to multiple Fusarium diseases at the seedling and adult growth stages and had important application value in breeding.

关键词： Seedling blight Stalk rot Multiple disease resistance

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Asymmetric reduction of conjugated C=C bonds by immobilized fusion of old yellow enzyme and glucose dehydrogenase

Green Synthesis and Catalysis

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Green Synthesis and Catalysis 2024年第2期5卷 80-87页

作者： Yongxing Li Pengqian luan Lele Dong Jianqiao Liu Luying Jiang Jing Bai Fufeng Liu Yanjun Jiang School of Chemical Engineering and Technology Hebei University of TechnologyTianjin 300130China College of Food Science and Biology Hebei University of Science&Technology26 Yuxiang StreetYuhua DistrictShijiazhuang 050018China Key Laboratory of Industrial Fermentation Microbiology of Ministry of Education Tianjin Key Laboratory of Industrial MicrobiologyCollege of BiotechnologyTianjin University of Science&TechnologyTianjin 300457China

Asymmetric reduction of the conjugated C=C bonds by the old yellow enzymes(OYEs)presents a promising field in the synthesis of chiral ***,few natural OYEs have been applied in large-scale applications due to the requirement of costly NADPH and low operational ***,a stable and efficient fusion of YqjM from Bacillus subtilis and glucose dehydrogenase(GDH)from Bacillus megaterium was constructed to stereoselectively reduce the conjugated C=C bonds in a self-sufficient continuous *** effects of the enzyme order and different linkers on the fusions were investigated by structural analysis and all-atom molecular dynamics *** best fusion YqjM_G_GDH gave 98% conversion of 100 mmol/L 2-methylcyclopentenone with an excellent ee value(>99%)in 3 h,while the mixture of individual enzymes only obtained 68% conversion after more than 8 *** improved substrate conversion of YqjM_G_GDH fusion was probably attributed to the increased flexibility of each fused enzyme and the shortening of the diffusion distance of NADPH regenerated.A one-pot process was designed to purify and immobilize the fusion on the Ni2t-nitrilotriacetic acid functionalized magnetic mesoporous silica *** resulting immobilized biocatalyst not only catalyzed the asymmetric reduction of various α,β-unsaturated ketones(20 mmol/L)continuously with only 50μmol/L NADPt to initiate the whole process,but also retained more than 82%of the initial activity after seven cycles,serving as a good candidate for the industrial applications.

关键词： Enzyme fusion Old yellowenzymes Asymmetricreduction C=C bond Cofactor regeneration Immobilization

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寒地城市老幼群体不同季节住区户外活动特征及其影响因素研究

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西部人居环境学刊 2024年第2期39卷 41-48页

作者：冷红栾佳艺袁青哈尔滨工业大学建筑与设计学院自然资源部寒地国土空间规划与生态保护修复重点实验室

住区是承载老幼群体户外活动的主要场所,寒地城市特殊气候条件下的季节变化对老幼群体的活动特征影响显著,本文根据其影响因素提出满足老幼群体全季节活动需求的共享型户外活动空间营造策略。利用实地调研与问卷结合的方式对哈尔滨市6... 详细信息

住区是承载老幼群体户外活动的主要场所,寒地城市特殊气候条件下的季节变化对老幼群体的活动特征影响显著,本文根据其影响因素提出满足老幼群体全季节活动需求的共享型户外活动空间营造策略。利用实地调研与问卷结合的方式对哈尔滨市6个样本住区452组老幼群体进行调查,研究不同季节老幼群体住区户外活动的时间与空间分布特征,并通过多元回归分析的方法分别提取不同季节下老幼群体活动频率、活动时长和活动类型的影响因素。基于研究结果,从优化目标、优化机制、环境营造三方面提出寒地住区户外活动空间的优化策略。

关键词：寒地城市老幼群体季节户外活动特征住区优化策略

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新型农村集体经济助力中国特色农业强国建设:作用机理与路径选择

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天津社会科学 2024年第3期 57-67页

作者：栾江中共天津市委党校经济学教研部

农业强国是社会主义现代化强国的根基,特殊的国情农情决定了我国要建设的农业强国既要有世界农业强国的一般特征,更要体现中国特色。新型农村集体经济作为社会主义公有制经济的重要实现形式,能够在中国特色农业强国建设中发挥重要作用,... 详细信息

农业强国是社会主义现代化强国的根基,特殊的国情农情决定了我国要建设的农业强国既要有世界农业强国的一般特征,更要体现中国特色。新型农村集体经济作为社会主义公有制经济的重要实现形式,能够在中国特色农业强国建设中发挥重要作用,包括通过发挥组织动员作用打造农业强国产业体系,发挥制度牵引作用确保农业强国建设方向,发挥文化建设作用丰富农业强国文化内涵等。当前,我国新型农村集体经济面临发展动力低下、市场化运营机制不健全、收益分配机制不完善、文化功能发挥不充分等问题。未来应进一步激发新型农村集体经济发展的内生动力、完善市场化运营机制和发展成果共享机制、提升共同富裕带动效果、强化文化功能建设,加强中国特色农业强国建设的物质保障力、主体支撑力、价值驱动力和文化影响力,加速推进农业农村现代化。

关键词：农业强国中国特色新型农村集体经济农业农村现代化

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基于全生命周期碳排放的海水贝藻养殖碳汇核算——以广东省湛江市为例

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中山大学学报（自然科学版）（中英文） 2024年第3期63卷 80-87页

作者：袁雪婷罗丽娟曾雪兰张远栾天罡广东省流域水环境治理与水生态修复重点实验室/广东工业大学生态环境与资源学院广东广州510006 化学与精细化工广东省实验室揭阳分中心广东揭阳515200 广东工业大学碳中和与绿色发展协同创新研究院广东广州510006 水产动物疫病防控与健康养殖全国重点实验室/中山大学生命科学学院广东广州510275

湛江市的海水养殖面积3.7万hm^(2),是广东省乃至全国重要的水产品生产和加工基地,对其进行海水贝藻养殖碳汇核算,为湛江市未来开展渔业碳汇交易,服务国家碳中和需求提供数据支持具有重要意义。本研究依据实地调查以及实验室分析检测获... 详细信息

湛江市的海水养殖面积3.7万hm^(2),是广东省乃至全国重要的水产品生产和加工基地,对其进行海水贝藻养殖碳汇核算,为湛江市未来开展渔业碳汇交易,服务国家碳中和需求提供数据支持具有重要意义。本研究依据实地调查以及实验室分析检测获得湛江市本土贝藻碳汇核算的关键性基础数据,并基于贝藻类全生命周期碳排放,精准评估湛江市海水贝藻养殖碳汇能力。研究结果表明,2021年湛江市海水养殖贝藻碳汇总量(以CO_(2)计)为155436 t,其中贝类155359 t,藻类77 t。按照我国目前蓝碳交易市场中CO_(2)交易价格,湛江市贝类和藻类碳汇创造的经济价值为1648万元。综合贝藻类全生命周期碳排放的方法,湛江市贝类净碳汇为135176 t,藻类为39 t。由此可见,湛江市具有较高的海水贝藻养殖碳汇能力,能带来可观的减排经济效益,为我国实现气候变化的解决方案以及完成“双碳”目标做出重要贡献。

关键词：蓝碳碳汇生命周期评价双壳贝类碳足迹

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Study on blood pressure rhythm in hypertensive patients with Yin deficiency syndrome and a random forest model for predicting hypertension with Yin deficiency syndrome

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Journal of Traditional Chinese Medicine 2024年第3期44卷 564-571页

作者： ZHOU Ying LI Ping luan Jianwei SHEN Rui WU Yinglan XU Qiwen WANG Xinyue ZHU Yao XU Xiangru LIU Zitian JIANG Yuning ZHONG Yong HE Yun JIANG Weimin Department of Cardiology the Affiliated Hospital of Nanjing University of Chinese MedicineJiangsu Province Hospital of Traditional Chinese Medicine Department of Health Management Centre Jinling HospitalAffiliated Hospital of Medical SchoolNanjing University Emergency Department Longhua HospitalShanghai University of Traditional Chinese Medicine Department of General Internal Medicine the Affiliated Hospital of Nanjing University of Chinese MedicineJiangsu Province Hospital of Traditional Chinese Medicine

OBJECTIVE: To investigate blood pressure rhythm(BPR)in Yin deficiency syndrome of hypertension(YDSH)patients and develop a random forest model for predicting ***: Our study was consistent with technical processes and specification for developing guidelines of Evidence-based Chinese medicine clinical practice(T/CACM 1032-2017). We enrolled 234 patients who had been diagnosed with primary hypertension without antihypertensive medications prior to the enrollment. All participants were divided into Yin deficiency group(YX, n = 74) and non-Yin deficiency group(NYX, n = 160).Participants were professionally grouped by three experienced chief Traditional Chinese Medicine(TCM)physicians according to four examinations(i.e.,inspection, listening and smelling, inquiry and palpation).We collected data on 24 h ambulatory blood pressure monitoring(ABPM) and YDSH rating scale. We divided 24 h of a day into 12 two-hour periods [Chen-Shi(7:00-9:00), Si-Shi(9:00-11:00), Wu-Shi(11:00-13:00), Wei-Shi(13:00-15:00), Shen-Shi(15:00-17:00), You-Shi(17:00-19:00), Xu-Shi(19:00-21:00), Hai-Shi(21:00-23:00), ZiShi(23:00-1:00), Chou-Shi(1:00-3:00), Yin-Shi(3:00-5:00), Mao-Shi(5:00-7:00)] according to the theory of “midnight-midday ebb flow”. We used random forest to build the diagnostic model of YDSH, with whether it was Yin deficiency syndrome as the outcome. RESULTS: Compared with NYX group, YX group had more female participants with older age, lower waist circumference, body mass index(BMI), diastolic blood pressure(DBP), and smoking and drinking rate(all P < 0.05). The YDSH rating scores of YX group [28.5(21.0-36.0)] were significantly higher than NYX group [13.0(8.0-22.0)](P < 0.001), and the typical symptoms of YX group included vexing heat in the chest, palms and soles, dizziness, dry eyes, string-like and fine pulse, soreness and weakness of lumbus and knees, palpitations, reddened cheeks, and tinnitus(all P < 0.05). The ratio of non-dipper hypertension in YX group was higher t

关键词： blood pressure rhythm blood pressure variability deficiency syndrome hypertension random forest

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挡风墙对风区隧道火灾排烟效果的影响研究

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铁道科学与工程学报 2024年第5期21卷 1979-1989页

作者：栾蝶范传刚中南大学土木工程学院湖南长沙410075 轨道交通工程结构防灾减灾湖南省重点实验室湖南长沙410075

烟气是隧道火灾中的最致命因素。针对处于风区的隧道受环境风影响,火灾烟气无法有效排出导致隧道内热量积聚和人员受困的问题,建议在风区隧道出口设置合理尺寸的挡风墙。以标准的2车道公路隧道为例,基于对公路隧道火灾载荷的调研,借助FD... 详细信息

烟气是隧道火灾中的最致命因素。针对处于风区的隧道受环境风影响,火灾烟气无法有效排出导致隧道内热量积聚和人员受困的问题,建议在风区隧道出口设置合理尺寸的挡风墙。以标准的2车道公路隧道为例,基于对公路隧道火灾载荷的调研,借助FDS(Fire Dynamics Simulator)软件建立典型的2车道公路隧道火灾模型,考虑风区10,20和30 m/s 3种横风速度,分别讨论有无挡风墙时的隧道内火灾烟气运动情况。无挡风墙时,横风会将烟气封堵在隧道内,造成隧道内热量的积聚,同时,隧道下部空间被烟气侵蚀,对人员逃生和救援工作产生不利的影响。进一步探讨不同宽度和高度的挡风墙对隧道内火灾烟气运动、隧道顶部温度、能见度及烟气层高度参数的影响,从而确定适合风区2车道公路隧道的最佳挡风墙尺寸。研究结果表明,隧道出口设置挡风墙可以有效促进风区隧道火灾烟气的排出,且能够大大降低隧道顶部温度。这是由于风流经过挡风墙时发生边界层分离,挡风墙内部形成负压驱使火灾烟气从隧道排出。当横风速度较大时,烟气倾向于全部从风区一侧排出,非风区一侧形成完全干净的空间,此时人员逃生和灭火救援可以从非风区侧进行。此外,挡风墙的尺寸并非越大越好,基于3个隧道火灾安全疏散指标,提出了优化的风区2车道公路隧道的挡风墙尺寸为4 m(宽)×8 m(高)。

关键词：隧道挡风墙横风烟气排出优化设计

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