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Synthesis of amphiphilic fluorinated Janus particles with applications in stabilizing surfactant-free foams

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Particuology 2018年第6期16卷 112-117页

作者： Gang wang Keliang wang ying wang Ministry of Education Key Laboratory of Enhanced Oil Recovery Technology Northeast Petroleum UniversityDaqing 163318China State Key Laboratory oflnorganic Synthesis &Preparative Chemistry Jilin UniversityChangchun 130012China

We report the preparation of amphiphilic Janus particles covered with fluorinated chains as well as the self-assembly and foam stabilization property of these particles over a limited range of *** structure and properties of these particles were characterized using scanning electron microscopy,Fourier transform infrared spectroscopy,fluorescence microscopy,pendant drop tensiometer,and *** a particle concentration of 0.6wt%,the adsorption of the Janus particles at the interface was saturated and an equilibrium surface tension of approximately 35mN/m was obtained. No further decreases in surface tension were observed with additional increases in the particle *** a concentration of 0.4wt%,the Gibbs stability criterion was achieved and the foam volume was almost constant throughout the observation ***,the liquid fraction of the foam exhibited a static equilibrium at each concentration assessed,demonstrating that drainage was arrested *** results confirm that the use of amphiphilic Janus particles is an efficient approach to stabilizing foams.

关键词： Janus particle Fluorinated grafting agent Amphiphilic particle Self-assembly Dynamic surface tension Foam stability

Characterization and Expression Analysis of a Complement Component Gene in Sea Cucumber(Apostichopus japonicus)

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Journal of Ocean University of China 2015年第6期14卷 1096-1104页

作者： CHEN Zhong ZHOU Zunchun YANG Aifu DONG ying guan Xiaoyan JIANG Bei wang Bai Liaoning Key Laboratory of Marine Fishery Molecular Biology Liaoning Ocean and Fisheries Science Research Institute

The complement system plays a crucial role in the innate immune system of animals. It can be activated by distinct yet overlapping classical, alternative and lectin pathways. In the alternative pathway, complement factor B(Bf) serves as the catalytic subunit of complement component 3(C3) convertase, which plays the central role among three activation pathways. In this study, the Bf gene in sea cucumber(Apostichopus japonicus), termed Aj Bf, was obtained by rapid amplification of c DNA ends(RACE). The full-length c DNA of Aj Bf was 3231 bp in length barring the poly(A) tail. It contained an open reading frame(ORF) of 2742 bp encoding 913 amino acids, a 105 bp 5'-UTR(5'-terminal untranslated region) and a 384 bp 3'-UTR. Aj Bf was a mosaic protein with six CCP(complement control protein) domains, a VWA(von Willebrand factor A) domain, and a serine protease domain. The deduced molecular weight of Aj Bf protein was 101 k Da. Quantitative real time PCR(q RT-PCR) analysis indicated that the expression level of Aj Bf in A. japonicus was obviously higher at larval stage than that at embryonic stage. Expression detection in different tissues showed that Aj Bf expressed higher in coelomocytes than in other four tissues. In addation, Aj Bf expression in different tissues was induced significantly after LPS or Poly I:C challenge. These results indicated that Aj Bf plays an important role in immune responses to pathogen infection.

关键词： sea cucumber Apostichopusjaponicus complement factor B LPS challenge PolyI:C challenge

Whey protein peptide PEW attenuates hyperuricemia and associated renal inflammation in potassium oxonate and hypoxanthine-induced rat

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Food Bioscience 2023年第1期51卷 846-857页

作者： Xiaofen Qi Yanfeng Ma Kaifang guan Chunhong Liu Rongchun wang ying Ma Tianjiao Niu School of Chemistry and Chemical Engineering Harbin Institute of TechnologyHarbin150001HeilongjiangChina Mengniu Hi-tech Dairy(Beijing)Co. LtdBeijing101107China

Pro-Glu-Trp(PEW),a whey protein-derived peptide,has been previously shown in vitro to have antihyperuricemic *** current study further evaluated the roles and the underlying mechanism of PEW in the management of hyperuricemia(HUA)in rat induced by potassium oxonate(PO)and *** revealed that PEW significantly reduced the levels of uric acid(UA),creatinine(Cr),and blood urea nitrogen(BUN)in serum,and effectively suppressed the activities of xanthine oxidase(XOD)associated with UA synthesis and modulated the expression of organic ion transporters related to UA ***,PEW alleviated UAinduced renal inflammation by regulating oxidative stress,suppressing the level of pro-inflammatory cytokines,and inhibiting the activation of NOD-like receptor family pyrin domain-containing 3(NLRP3)inflammasome and Toll-like receptor 4/myeloid differentiation factor 88/NF-kappaB(TLR4/MyD88/NF-κB)signaling *** together,these relults indicated that PEW improved HUA and renal inflammation by inhibiting UA synthesis,promoting renal UA excretion and suppressing NLRP3 inflammasome and TLR4/MyD88/NF-κB signaling pathways.

关键词： Peptide Hyperuricemia Organic ion transporters Oxidative stress NLRP3 inflammasome TLR4/MyD88/NF-κB signaling pathway

A Mean-Field Optimal Control for Fully Coupled Forward-Backward Stochastic Control Systems with Lévy Processes

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Journal of Systems Science & Complexity 2022年第1期35卷 205-220页

作者： HUANG Zhen wang ying wang Xiangrong College of Mathematics and Systems Science Shandong University of Science and TechnologyQingdao 266590China.

This paper is concerned with a class of mean-field type stochastic optimal control systems,which are governed by fully coupled mean-field forward-backward stochastic differential equations with Teugels martingales associated to Lévy *** these systems,the coefficients contain not only the state processes but also their marginal distribution,and the cost function is of mean-field type as *** necessary and sufficient conditions for such optimal problems are ***,the applications to the linear quadratic stochastic optimization control problem are investigated.

关键词： Adjoint equation Lévy processes mean-field forward-backward stochastic differential equations stochastic maximum principle Teugels martingales

Influence of Nucleotide-biased Fluorescence Emissions of SYBR Green Ⅱ on the Result Consistence of Rolling Circle Amplification

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Chemical Research in Chinese Universities 2019年第6期35卷 1119-1123页

作者： ZHANG Bin JIANG Jiquan YUAN ying guan Yifu Department of Biochemistry and Molecular Biology China Medical UniversityShenyang 110122P.R.China Department of Plastic Surgery School of StomatologyChina Medical UniversityShenyang 110009P.R.China Department of Biochemistry and Molecular Biology College of Medical Laboratory and TechnologyHarbin Medical University(Daqing)Daqing 163000P.R.China

The fluorescence dye SYBR Green Ⅱ(SG Ⅱ)has been frequently used in rolling circle amplification(RCA)based analyses of nucleic ***,a good amount of inconsistencies have been reported in regards the quality and reproducibility of RCA *** properly examine this experimental issue,here we utilized a series of synthetic oligonucleotides and circular templates to investigate the impact of SG Ⅱ in RCA *** results indicate that SG Ⅱ enables a strong fluorescence signal only when complexing with guanosine(G)*** RCA reactions,long single-stranded RCA products,enriched with G residues,result in higher fluorescence emission when compared with the addition of other nucleotide *** results suggest that the nucleotide composition of the reaction can affect the amplification results and,eventually,can lead to inconsistent fluorescence of the RCA *** work indicates that particular attention should be given when circular templates are designed for the quantitative analysis of nucleic acids,to further allow the signal reproducibility of RCA-based experiments.

关键词： Rolling circle amplification SYBR Green Ⅱ Nucleotide-biased Fluorescence emission

Oxidation behavior of Ni-based superalloy GH738 in static air between 800 and 1000℃

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Rare Metals 2021年第3期40卷 616-625页

作者： Jue wang Hao Xue ying wang Jiangsu Key Laboratory of Advanced Structural Materials and Application Technology Nanjing 211167China School of Materials Science and Engineering Nanjing Institute of TechnologyNanjing 211167China

The oxidation behavior of a nickel-based superalloy GH738 was studied by isothermal oxidation tests in still air at different temperatures,with exposure time up to 100 ***-kinetic curves were plotted using the mass gain *** surface and cross-sectional morphologies were observed by scanning electron microscopy(SEM).A composition analysis and an oxidation-product identification were conducted using energydispersive X-ray spectroscopy(EDS) and an X-ray diffraction(XRD),*** results showed that GH738 exhibited parabolic oxidation-kinetic curves,with stable parabolic-rate constants at each *** activation energy of oxide growth was calculated to be329.6 kJ·mol^(-1).Cr_(2) O_(3)(chromia) was the external oxidation product at 800℃.A TiO_(2)-Cr_(2) O_(3) double-layer structure was formed at 900℃.The position of TiO_(2) changed from the oxide-metal interface to the air-oxide interface by the diffusion of Ti atoms in chromia during the oxidation time at this *** was observed in the Cr_(0.12)Ti_(0.78)O_(1.74)-Cr_(2) O_(3) multi-layer oxide of at 1000℃,which increased the oxidation *** all the tests,the main internal oxide was always Al_(2) O_(3).The entire GH738 oxidation process was interpreted by the competitive diffusion of elements(Cr,Ti,Ni,etc.) in metal matrix and chromia,while a schematic diagram of oxidation process was proposed.

关键词： GH738 Oxidation behavior Diffusion Multilayer structure

Effects of different conditions on Pb^(2+) adsorption from soil by irrigation of sewage in South China

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Journal of Central South University 2012年第1期19卷 213-221页

作者： HUANG guan-xing ZHANG ying SUN Ji-chao JING Ji-hong LIU Jing-tao wang ying Institute of Hydrogeology and Environmental Geology Chinese Academy of Geological SciencesShijiazhuang 050061China

Pb2＋ adsorption onto a soil by irrigation of sewage in the Pearl River Delta of South China was examined as a function of the reaction time, solution pH, initial lead concentration, organic matter （humic acid） and competitive ions （Cu2＋）. The adsorption of Pb2＋ onto the soil was investigated on batch equilibrium adsorption experiments. Results show that the Pb2＋ adsorption on the soil is relatively rapid in the first 30 min and reaches equilibrium at 2 h, and the kinetics of the adsorption process on the soil is well characterized by the pseudo-second order reaction rate. Langmuir, Freundlich and Temkin isothermal models are fit for the adsorption of Pb2＋ onto the soil, and the maximum amount of Pb2＋ adsorption （Qm） is 7.47 mg/g. The amount of Pb2＋ adsorption increases with increasing the pH at the range of 1.2-4.5 and reaches a plateau at the range of 4.5-12. The presence of humic acid in soil decreases the adsorption of Pb2＋ onto the soil at solution pH of 8 since the negatively charged humic acid with Pb2＋ is difficult to be adsorbed on the negatively charged soil surface. The adsorption of Pb2＋ onto the soil also decreases in the presence of Cu2＋ due to file competition adsorption between Pb2＋ and Cu2＋.

关键词： adsorption pb2+ soil pH humic acid

Intelligent Electrocardiogram Analysis in Medicine:Data,Methods,and Applications

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Chinese Medical Sciences Journal 2023年第1期38卷 38-48页

作者： Yu-Xia guan ying An Feng-Yi Guo Wei-Bai Pan Jian-Xin wang Hunan Provincial Key Lab on Bioinformatics School of Computer Science and EngineeringCentral South UniversityChangsha 410083China Big Data Institute Central South UniversityChangsha 410083China

Electrocardiogram(ECG)is a low-cost,simple,fast,and non-invasive *** can reflect the heart’s electrical activity and provide valuable diagnostic clues about the health of the entire ***,ECG has been widely used in various biomedical applications such as arrhythmia detection,disease-specific detection,mortality prediction,and biometric *** recent years,ECG-related studies have been carried out using a variety of publicly available datasets,with many differences in the datasets used,data preprocessing methods,targeted challenges,and modeling and analysis *** we systematically summarize and analyze the ECGbased automatic analysis methods and ***,we first reviewed 22 commonly used ECG public datasets and provided an overview of data preprocessing *** we described some of the most widely used applications of ECG signals and analyzed the advanced methods involved in these ***,we elucidated some of the challenges in ECG analysis and provided suggestions for further research.

关键词： Electrocardiogram database preprocessing machine learning medical big data analysis

呋虫胺和唑虫酰胺在茶叶加工过程中的残留特征及膳食风险评估

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食品科学 2025年第1期46卷 192-199页

作者：李春勇吕莹金静王霞秦曙山西农业大学山西功能农产品检验检测中心山西太原030031

为明确呋虫胺和唑虫酰胺微乳剂在茶叶种植、烘干、浸泡过程中的残留特征,在山东、浙江等10地开展规范农药登记残留田间试验,并进行加工因子校正膳食风险评估模型比较研究。样品经体积分数5%甲酸-乙腈提取、盐析分层、分散固相萃取净化后... 详细信息

为明确呋虫胺和唑虫酰胺微乳剂在茶叶种植、烘干、浸泡过程中的残留特征,在山东、浙江等10地开展规范农药登记残留田间试验,并进行加工因子校正膳食风险评估模型比较研究。样品经体积分数5%甲酸-乙腈提取、盐析分层、分散固相萃取净化后,经超高效液相色谱-串联质谱法检测,外标法定量。结果表明:在0.005~0.500μg/mL范围内,呋虫胺及其代谢物、唑虫酰胺质量浓度与其响应峰面积呈良好线性关系(R^(2)>0.99),在0.05、1.00、20.00、50.00 mg/kg(茶鲜叶、茶干叶),0.05、0.10、5.00 mg/kg(茶汤)添加水平下,呋虫胺及其代谢物1-甲基-3-[(3-四氢呋喃)甲基]脲、1-甲基-3-[(3-四氢呋喃)甲基]二氢胍盐的平均加标回收率为74%~103%,相对标准偏差为1%~9%;唑虫酰胺的平均加标回收率为75%~95%,相对标准偏差为1%~6%,方法定量限均为0.05 mg/kg;20%呋虫胺·唑虫酰胺微乳剂按推荐剂量施药后,呋虫胺(含代谢物)在茶鲜叶中的规范残留试验中值(supervised trials median residue,STMR)经烘干因子校正后与茶干叶中STMR相应的国家估算每日摄入量(national estimated daily intake,NEDI)均为4.08 mg,占每日允许摄入量(allowable daily intake,ADI)的32.4%;唑虫酰胺在茶鲜叶中的STMR经烘干因子校正后的NEDI为0.17581 mg,占ADI的46.5%,茶干叶中STMR对应的NEDI为0.18925 mg,占ADI的50.1%,呋虫胺和唑虫酰胺在不同评估模型下获得的长期膳食暴露风险均低于100%;呋虫胺(含代谢物)经加工因子校正后的国际短期膳食摄入量(international estimation of short-term dietary intake,IESTI)与校正前处于同一风险水平,低于急性参考剂量(acute reference dose,ARfD)(1 mg/kg);唑虫酰胺的IESTI为3.46×10^(-4) mg/kg(采收间隔7 d),低于ARf D(0.01 mg/kg),本研究方法在茶汤中未检测到唑虫酰胺残留,故未得到浸泡因子及相应的IESTI。综上,按推荐剂量在茶树上使用20%呋虫胺·唑虫酰胺微乳剂是安全的,最终残留量对应的长期及急性膳食摄入风险均在可接受范围,利用加工因子校正评估模型更切合实际风险水平。

关键词：呋虫胺唑虫酰胺茶叶加工因子残留特征风险评估