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Ultra-high spatio-temporal resolution imaging with parallel acquisition-readout structured illumination microscopy(PAR-SIM)

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Light(Science & Applications) 2024年第6期13卷 1140-1158页

作者： xinzhu Xu Wenyi Wang Liang Qiao Yunzhe Fu xichuan Ge Kun Zhao Karl Zhanghao Meiling {2. xin chen Meiqi Li Dayong Jin Peng xi department of Biomedical engineering College of Future TechnologyPeking UniversityBeijing100871China Wallace H.Coulter Dept.of Biomedical engineering Georgia Institute of Technology and Emory UniversityAtlanta30332GAUSA National Biomedical Imaging Center Peking UniversityBeijing100871China department of Biomedical engineering College of EngineeringSouthern University of Science and TechnologyShenzhen518055GuangdongChina Airy Technologies Co. Ltd.Beijing100086China Eastern Institute for Advanced Study Eastern Institute of TechnologyNingboZhejiang315200China School of Life Science Peking UniversityBeijing100871China Institute for Biomedical Materials and Devices(IBMD) Faculty of ScienceUniversity of Technology SydneySydneyNSW2007Australia

Structured illumination microscopy(SIM)has emerged as a promising super-resolution fluorescence imaging technique,offering diverse configurations and computational strategies to mitigate phototoxicity during real-time imaging of biological *** efforts to enhance system frame rates have concentrated on processing algorithms,like rolling reconstruction or reduced frame reconstruction,or on investments in costly sCMOS cameras with accelerated row readout *** this article,we introduce an approach to elevate SIM frame rates and region of interest(ROI)coverage at the hardware level,without necessitating an upsurge in camera expenses or intricate ***,parallel acquisition-readout SIM(PAR-SIM)achieves the highest imaging speed for fluorescence imaging at currently available detector *** using the full frame-width of the detector through synchronizing the pattern generation and image exposure-readout process,we have achieved a fundamentally stupendous information spatial-temporal flux of 132.9 MPixels·s^(−1),9.6-fold that of the latest techniques,with the lowest SNR of−2.11 dB and 100 nm ***-SIM demonstrates its proficiency in successfully reconstructing diverse cellular organelles in dual excitations,even under conditions of low signal due to ultra-short exposure ***,mitochondrial dynamic tubulation and ongoing membrane fusion processes have been captured in live COS-7 cell,recorded with PAR-SIM at an impressive 408 *** posit that this novel parallel exposure-readout mode not only augments SIM pattern modulation for superior frame rates but also holds the potential to benefit other complex imaging systems with a strategic controlling approach.

关键词： hardware illumination resolution

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RANDOM COpolymer OF PROPYLENE OxiDE AND ETHYLENE OxiDE PREPARED BY DOUBLE METAL CYANIDE COMPLEX CATALYST

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Chinese Journal of polymer Science 2002年第5期20卷 453-459页

作者： Yi-jun Huang Guo-rong Qi guan-xi {2. department of polymer Science and engineering, zhejiang university Hangzhou 31002.,China zhejiang Univ Dept Polymer Sci & Engn Hangzhou 310027 Peoples R China

Copolymerization of propylene oxide (PO) and ethylene oxide (EO) using double metal cyanide (DMC) complex as the catalyst was carried out. The structure of random copolymers was confirmed by C-13-NMR and IR spectra. H-1-NMR analysis shows that the EO content in the copolymer is the same as that in the initial monomer feed. Moderate molecular weight copolymers with various EO content were obtained and their values of molecular weight distribution (MWD) fell in the range of 1.2.-1.55. It was found that the molecular weight of copolymers is controlled by the mass ratio of EO+PO to initiator moles used, The reaction rate as well as polymer yield decrease with increasing EO content in the feed composition.

关键词： propylene oxide ethylene oxide double metal cyanide complex

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Tailored wrinkles for tunable sensing performance by stereolithography

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Interdisciplinary Materials 2024年第3期3卷 414-424页

作者： Ruiyi Jiang Jie Pu Yuxuan Wang Jipeng chen Gangwen Fu Xue chen Jiayu Yang Jianghua Shen {2.ng Sun Jun Ding {2. Xu Key Laboratory of Flexible Electronics of zhejiang Province Ningbo Institute of Northwestern Polytechnical UniversityNingboChina Frontiers Science Center for Flexible Electronics Institute of Flexible ElectronicsNorthwestern Polytechnical UniversityXi'anChina Research&Development Institute of Northwestern Polytechnical University in ShenzhenNanshan DistrictShenzhenChina Affiliated Hospital of Nanjing university of Chinese Medicine NanjingChina School of Aeronautics Northwestern Polytechnical UniversityXi'anChina Combustion Internal Flow and Thermal-Structure LaboratorySchool of AstronauticsNorthwestern Polytechnical UniversityXi'anChina department of Materials Science and engineering Faculty of EngineeringNational University of SingaporeSingapore

Conducting polymer hydrogel can address the challenges of stricken biocompatibility and ***,conventional conducting polymer hydrogels are often brittle and weak due to the intrinsic quality of the material,which exhibits *** property may cause a delay in sensor response time due to *** overcome these limitations,we have designed a wrinkle morphology three-dimensional(3D)substrate using digital light processing technology and then followed by in situ polymerization to form interpenetrating polymer network *** novel design results in a wrinkle morphology conducting polymer hydrogel elastomer with high precision and geometric freedom,as the size of the wrinkles can be controlled by adjusting the treating *** wrinkle morphology on the conducting polymer hydrogel effectively reduces its viscoelasticity,leading to samples with quick response time,low hysteresis,stable cyclic performance,and remarkable resistance ***,the 3D gradient structure augmented the sensor's sensitivity under minimal stress while exhibiting consistent sensing *** properties indicate the potential of the conducting polymer hydrogel as a flexible sensor.

关键词： 3D printing conducting polymer hydrogel flexible sensor micro-wrinkle structure

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Optical Properties of Fullerenated Poly(epoxy propyl carbazole)

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Chinese Chemical Letters 1998年第9期9卷 835-837页

作者： Tao GU Wan xi chen Zhu De XU ({2. of Chemistry, zhejiang university, Hangzhou 31002.) zhejiang Univ Dept Chem Hangzhou 310027 Peoples R China

The optical properties of the fullerenated poly(epoxy propyl carbazole) (PEPC) have been investigated. Compared with pure PEPC, fullerenated PEPC has the same structure in the UV region of the absorption spectrum, and extends the active range to a longer wavelength. The apparent quenching of PEPC fluorescence was observed in the fullerenated polymer and it can be attributed to intramolecular excited state energy transfer.

关键词： fullerene (fullerenated) PEPC (poly(epoxy propyl carbazole)) optical properties

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IN SITU INTERFEROMETRIC STUDY ON THE GELATION PROCESS OF POLYACRYLIC ACID GELS

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Chinese Journal of polymer Science 2000年第4期18卷 373-377页

作者： Ying guan Qiang chen {2.an-min Zhang Yu-{2.ng Peng Jian Xu polymer Physics Laboratory, Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China department of Information & Electronic engineering, zhejiang university, Hangzhou 31002., China chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, chengdu 610041, China Chinese Acad Sci Inst Chem Ctr Mol Sci Polymer Phys Lab Beijing 100080 Peoples R China zhejiang Univ Dept Informat & Elect Engn Hangzhou 310027 Peoples R China Chinese Acad Sci Chengdu Inst Organ Chem Chengdu 610041 Peoples R China

In situ interferometry was used to investigate the gelation process of polyacrylic acid (PAA) gels. The basic principle of the in situ interferometry technique is illustrated. It can give sufficient information for non-destructive and successful investigation of the whole gelation process. The effect of initiator concentration on the gelation process was studied. The polymerization rate of AA increases with increasing initiator concentration. The error arising from the thermal effect in the gelation process can be neglected.

关键词： in situ interferometry gelation polyacrylic acid

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Recent Advances on Surface-modified Biomaterials Promoting Selective Adhesion and Directional Migration of Cells

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Chinese Journal of polymer Science 2021年第7期39卷 815-823页

作者： chen-xi Tu Chang-You Gao MOE Key Laboratory of Macromolecular Synthesis and Functionalization Department of Polymer Science and EngineeringZhejiang UniversityHangzhou 310027China

The surfaces and interfaces of biomaterials interact with the biological systems in multi-scale levels,and thereby influence the biological functions and comprehensive performance in vitro and in *** particular,a surface promoting the selective adhesion and directional migration of desired types of cells in complex environment is extremely important in the repair and regeneration of tissues such as peripheral nerve and blood vessel,and long-term application of intracorporal devices such as intravascular ***,surface modification of biomaterials is a facile and effective method to achieve the desired cell-biomaterials *** this short review,recent advances on the surface modification of biomaterials to regulate selective cell adhesion and migration are briefly *** particular,the surface properties of biomaterials are manipulated via the convenient introduction of amino groups to the ester-based polymers,the formation of polyelectrolyte multilayers,and the fabrication of topology and gradient cues,etc.,followed by the association of chemical and biological signals such as collagen,heparin,hyaluronic acid,peptides and cell growth *** selective adhesion and directional migration of various types of cells such as endothelial cells(ECs),smooth muscle cells(SMCs),hepatocytes and Schwann cells(SCs)are achieved over the competitive counterpart cells by the use of cell-resisting substances and cell-selective motifs on gradient substrates in most *** advances on cell behaviors in three-dimensional(3D)ceH-extracellular matrix(ECM)-mimicking substrates are also reviewed.

关键词： Biomaterials Surface modification Gradient Cell migration Cell selectivity

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超96%近红外光隔热率的高性能半透明有机太阳能电池

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高分子学报 2024年第9期55卷 1134-1144页

作者：李耀凯关诗陶李水兴左立见陈红征浙江大学硅及先进半导体材料全国重点实验室、高分子科学与工程学系杭州310027 浙江大学杭州国际科创中心信息与功能材料研究院杭州311200

半透明光伏是一种广受关注的太阳能光电转换技术,在吸光供能的同时,还可以保留一定的透光性.但半透明有机太阳能电池(semitransparent organic solar cells,STOPV)要想实现应用,必须解决能量转换效率(power conversion efficiency,PCE)... 详细信息

半透明光伏是一种广受关注的太阳能光电转换技术,在吸光供能的同时,还可以保留一定的透光性.但半透明有机太阳能电池(semitransparent organic solar cells,STOPV)要想实现应用,必须解决能量转换效率(power conversion efficiency,PCE)、可见光透过率(average photopic transmittance,APT)与近红外光隔热率(infrared-light rejection rate,IRR)有机统一的问题.不同于传统的单一优化策略,本文工作通过采用高性能三元策略和多层光学调控层的方法来协同改善STOPV的性能.材料层面,通过结合宽带隙给体和2.吸收相似但对称性不同的近红外受体分子,将单结三元不透明有机太阳能电池的效率优化至18.66%.器件层面,提出三重光学调控方案,同时引入减反射层(anti-reflection layer,ARC),二氧化碲(tellurium dioxide,TeO_(2.)光学层和带通滤光片(bandpass filter,BF),来优化器件的透光性.最终制备的最佳STOPV,其PCE和APT分别达到了12.82.和35.70%,对应的光利用效率(light utilization efficiency,LUE)为4.6%,并且近红外光隔热率达到96.8%,实现了PCE、APT和IRR的均衡提升.本文提出了一种开发设计高性能STOPV的有效方案,有利于进一步推动STOPV的商业化应用进展.

关键词：半透明有机太阳能电池光学调控二氧化碲近红外光阻隔光电建筑集成

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Recent advances in plasmonic organic photovoltaics

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Science China Chemistry 2015年第2期58卷 210-220页

作者： xi Yang Wenqing Liu Hongzheng chen State Key Laboratory of Silicon Materials Key Laboratory of Macromolecular Synthesis and Functionalization Ministry of Education department of polymer Science and {2. Zhejiang University

Light trapping based on the localized surface-plasmon resonance(LSPR)effect of metallic nanostructures is a promising strategy to improve the device performance of organic solar cells(OSCs).We review recent advances in plasmonic-enhanced OPVs with solution-processed metallic nanoparticles(NPs).The different types of metallic NPs(sizes,shapes,and hybrids),incorporation positions,and NPs with tunable resonance wavelengths toward broadband enhancement are systematically summarized to give a guideline for the realization of highly efficient plasmonic photovoltaics.

关键词： plasmonic effect organic solar cell enhanced light absorption

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RATE OF INTRA-MOLECULAR CONTACT FORMATION IN SHORT TWO-DIMENSIONAL COMPACT polymerS CHAINS

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Chinese Journal of polymer Science 2006年第1期24卷 13-19页

作者： Jin chen Lin-xi Zhang A-gen xia department of Physics Zhejiang University Hangzhou 310027 China department of Physics Wenzhou Normal College Wenzhou 325027 China

It is important to know the rate of intra-molecular contact formation in proteins in order to understand how proteins fold clearly. Here we investigate the rate of intra-molecular contact formation in short two-dimensional compact polymer chains by calculating the probability distribution p（r） of end-to-end distance r using the enumeration calculation method and HP model on two-dimensional square lattice. The probability distribution of end-to-end distance p（r） of short two-dimensional compact polymers chains may consist of two parts, i.e. p（r） = p1（r）＋ p2.r）, where p1（r） and p2.r） are different for small r. The rate of contact formation decreases monotonically with the number of bonds N, and the rate appro{2.mately conforms to the scaling relation of k（N）∝ N^-α. Here the value of α increases with the contact radius a and it also depends on the percentage of H （hydrophobic） residues in the sequences of compact chains and the energy parameters of ^εНН、 ^εНН and ^εpp. Some comparisons of theoretical predictions with experimental results are also made. This investigation may help us to understand the protein folding.

关键词： Two-dimensional compact polymer HP model Rate of contact formation.

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Lightweight foam-like nitrogen-doped carbon nanotube complex achieving highly efficient electromagnetic wave absorption

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Journal of Materials Science & Technology 2024年第1期168卷 114-123页

作者： Zongcheng Li Jin Liang Zhiheng Wei xin Cao Jiahui Shan Chunwei Li xiaoyi chen Dong Zhou Ruizhe xing Chunjia Luo Jie Kong MOE Key Lab of Materials Physics and Chemistry in Extraordinary Conditions Shaanxi Key Laboratory of Macromolecular Science and TechnologySchool of Chemistry and Chemical EngineeringNorthwestern Polytechnical UniversityXi’an 710072China Key laboratory of Flexible Electronics of zhejiang Province Ningbo Institute of Northwestern Polytechnical UniversityNingbo 315103China polymer Materials and engineering {2. School of Materials Science and EngineeringChang’an UniversityXi’an 710064China

With the increased electromagnetic wave(EMW)threat to military and human health,the develop-ment of EMW-absorbing materials is ***-organic framework derivatives containing magnetic nanoparticles and a carbon matrix are potential candidates for designing efficient EMW-absorbing ***,a zeolitic imidazolate framework-67(ZIF-67)-embedded three-dimensional melamine foam is pyrolyzed to afford carbon foam-based nitrogen-doped carbon nanotube composites,named 3D foam-like CoO/Co/*** CoO/Co particles are confined in the dielectric carbon nanotube *** carbon nanotubes provide considerable conductive loss,while CoO/Co magnetic particles are con-ducive to providing magnetic loss and adjusting impedance ***,the numerous defect structures introduced by heteroatomic doping(nitrogen)cause dipole polarization and simultaneously adjust impedance ***,the unique porous nanotube structure promotes multiple re-flections and scattering of EMWs,further optimizing impedance ***/Co/N-CNTs composites exhibit a minimum reflection loss of−52.3 dB at a matching thickness of 2.0 mm,while the correspond-ing effective absorption bandwidth is 5.2. GHz at a matching thickness of 2.2.*** study reports a novel approach to fabricating a lightweight high-performance EMW-absorbing material.

关键词： Lightweight porous carbon materials Carbon nanotubes CoO/Co magnetic particles Electromagnetic synergistic effect Impedance matching

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