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Upregulation of GLT25D1 in Hepatic Stellate Cells Promotes Liver Fibrosis via the TGF-β1/SMAD3 Pathway In Vivo and In vitro

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Journal of Clinical and Translational Hepatology 2023年第1期11卷 1-14页

作者： Shiwei Wang Lingling He fan Xiao Meixin Gao Herui wei Junru Yang Yang Shu Fuyang Zhang Xiaohui Ye Ping Li Xiaohua Hao Xingang Zhou Hongshan wei Department of Gastroenterology Beijing Ditan HospitalCapital Medical UniversityBeijingChina Institute of Infectious Diseases Beijing Ditan HospitalCapital Medical UniversityBeijingChina Department of Gastroenterology Beijing Huaxin Hospitalthe First Affiliated Hospital of Tsinghua UniversityBeijingChina Beijing Ditan Hospital Capital Medical UniversityBeijingChina Department of Pathology Beijing Ditan HospitalCapital Medical UniversityBeijingChina Department of Gastroenterology Peking University Ditan Teaching HospitalBeijingChina

Background and Aims:Collagenβ(1-O)galactosyltransferase 25 domain 1(GLT25D1)is associated with collagen production and glycosylation,and its knockout in mice results in embryonic ***,its role in liver fibrosis remains elusive,particularly in hepatic stellate cells(HSCs),the primary collagen-producing cells associated with liver ***,we aimed to elucidate the role of GLT25D1 in ***:Bile duct ligation(BDL)-induced mouse liver fibrosis models,primary mouse HSCs(mHSCs),and transforming growth factor beta 1(TGF-β1)-stimulated LX-2 human hepatic stellate cells were used in in vivo and in vitro *** LX-2 cell lines with either GLT25D1 overexpression or knockdown were established using lentiviral ***-seq was performed to investigate the genomic ***/MS were used to identify glycosylation *** electronic microscopy(SEM)and second-harmonic generation/two-photon excited fluorescence(SHG/TPEF)were used to image collagen fibril ***:GLT25D1 expression was upregulated in nonparenchymal cells in human cirrhotic liver ***,its knockdown attenuated collagen deposition in BDL-induced mouse liver fibrosis and inhibited mHSC ***25D1 was overexpressed in activated versus quiescence LX-2 cells and regulated in vitro LX-2 cell activation,including proliferation,contraction,and ***25D1 also significantly increased liver fibrogenic gene and protein ***25D1 upregulation promoted HSC activation and enhanced collagen expression through the TGF-β1/SMAD signaling *** spectrometry showed that GLT25D1 regulated the glycosylation of collagen in HSCs,affecting the diameter of collagen ***:Collectively,the upregulation of GLT25D1 in HSCs promoted the progression of liver fibrosis by affecting HSCs activation and collagen stability.

关键词： Collagen Glycosylation Hepatic stellate cells Liver fibrosis TGF-β1

Helicobacter pylori inhibits the cleavage of TRAF1 via a Cag A-dependent mechanism

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World Journal of Gastroenterology 2016年第48期22卷 10566-10574页

作者： Xiu-Kun Wan Sheng-Ling Yuan Yan-Chun Wang Hao-Xia Tao wei Jiang Zhang-Yan guan Cheng Cao Chun-Jie Liu State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Biotechnology

AIM To study the impact on cleavage of tumor necrosis factor receptor-associated factor 1(TRAF1) regulated by Helicobacter pylori(H. pylori).METHODS Cleavage of TRAF1 was detected by western blotting in the human gastric cancer cell line AGS following treatment with an apoptosis inducer. Cleavage of TRAF1 mediated by caspase was examined in vitro using specific caspase inhibitors. The effect of the COOH-terminal TRAF1 fragment on gastric cell apoptosis during H. pylori infection was measured using flow cytometry. The impact of H. pylori infection on TRAF1 cleavage was detected in the presence of apoptosis inducer. The roles of H. pylori virulence factors that may regulate TRAF1 cleavage were analyzed using isogenic cag A-, vac A- and cag E- null *** TRAF1 was found to be cleaved in AGS cells treated with the apoptosis inducer, and caspase-8 was the major caspase involved in the cleavage of TRAF1. The COOH-terminal TRAF1 fragment significantly induced cell apoptosis(P < 0.05) as well as promoted H. pylori-induced cell apoptosis(P < 0.05). H. pylori infection was found to significantly inhibit the cleavage of TRAF1 and to inhibit the activation of caspase-8 in thepresence of the apoptosis inducer at specific infection times and different cell/bacteria ratios. We also found that the effects of cag E- and cag A- null mutants on the inhibition of TRAF1 cleavage and activation of caspase-8 were significantly attenuated, compared with wild-type H. pylori, in the presence of the apoptosis inducer, showing that the virulence factor Cag A was mainly involved in the inhibition of TRAF1 *** H. pylori infection significantly inhibits the cleavage of TRAF1 via a CagA-dependent mechanism, which would increase the relative amounts of full-length TRAF1 and exert an antiapoptotic effect on H. pylori-infected cells.

关键词： Helicobacter pylori Tumor necrosis factor receptor-associated factor 1 CagA Cleavage Apoptosis

Effects of inter-culture, arabinogalactan proteins, and hydrogen peroxide on the plant regeneration of wheat immature embryos

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Journal of Integrative Agriculture 2015年第1期14卷 11-19页

作者： ZHANG wei WANG Xin-min fan Rong YIN Gui-xiang WANG Ke DU Li-pu XIAO Le-le YE Xing-guo National Key Facility of Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences Chinese Academy of Agricultural Sciences Division of Agricultural and Environmental Science School of BiosciencesUniversity of Nottingham

The regeneration rate of wheat immature embryo varies among genotypes, howbeit many elite agriculture wheat varieties have low regeneration rates. Optimization of tissue culture conditions and attempts of adding signal molecules are effective ways to increase plant regeneration rate. Inter-culture is one of ways that have not been investigated in plant tissue culture. Moreover, the use of arabinogalactan proteins （AGPs） and hydrogen peroxide （H202） have been reported to increase regeneration rate in a few plant species other than wheat. The current research pioneeringly uses inter-culture of immature embryos of different wheat genotypes, and also investigates impacts of AGP and H2O2 on the induction of embryogenic calli and plant regeneration. As a result, high-frequency regeneration wheat cultivars Kenong 199 （KN 199） and Xinchun 9 （XC9）, together with low-frequency regeneration wheat line Chinese Spring （CS）, presented striking increase in the induction of embryogenic calli and plant regeneration rate of CS through inter-culture strategy, up to 52.19 and 67.98%, respectively. Adding 50 to 200 mg L-1 AGP or 0.005 to 0.01‰ H2O2 to the callus induction medium, enhanced growth of embryogenic calli and plant regeneration rate in quite a few wheat genotypes. At 50 mg L-1 AGP application level in callus induction medium plant regeneration rates of 8.49,409.06 and 283.16% were achieved for Jimai 22 （JM22）, Jingdong 18 （JD18） and Yangmai 18 （YM18）, respectively; whereas at 100 mg L-1 AGP level, CS （105.44%）, Chuannong 16 （CN16）（80.60%） and Ningchun 4 （NC4）（62.87%） acted the best. Moreover CS （79.05%）, JM22 （7.55%）, CN16 （101.87%）, YM18 （365.56%）, Yangmai 20 （YM20）（10.48%）, and CB301 （187.40%） were more responsive to 0.005 %o of H2O2, and NC4 （35.37%） obtained the highest shoot regeneration rates at 0.01%o of H2O2. Overall, these two methods, inter-culture and AGP （or H2O2） application, can be further applied to wheat transgenic research.

关键词： wheat immature embryos plant regeneration inter-culture arabinogalactan proteins hydrogen peroxide

Response of human epidermal growth factor receptor 2-positive colorectal cancer to lapatinib monotherapy: A case report

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World Journal of Gastrointestinal Oncology 2020年第9期12卷 1065-1072页

作者： Ji-Lin guan Jian-Hua Liu Qing Wang Yu-wei Cong Yao-Xu Chen Ke-Fei Huang Meng-Li Huang Ling Huang Department of Oncology Guangdong Provincial People's Hospital and Guangdong Academy of Medical SciencesGuangzhou 510655Guangdong ProvinceChina The Medical Department 3D Medicines Inc.Shanghai 201114China The Bioinformatic Department 3D Medicines Inc.Shanghai 201114China

BACKGROUND Human epidermal growth factor receptor 2(HER2)amplification is a molecular driver for a subset of colorectal cancers(CRCs)and one of the major causes of anti-epidermal growth factor receptor(EGFR)treatment *** to dual anti-HER2 treatments,which have been shown to be effective in HER2-positive metastatic CRC patients,single-agent anti-HER2 therapy is rarely used to treat *** SUMMARY Herein,we report a case of RAS/BRAF-wild-type metastatic CRC that was identified as HER2-positive through circulating tumor DNA(ctDNA)testing by next-generation sequencing following the failure of two lines of ***,the patient was given lapatinib monotherapy that led to a partial response with a progression-free survival of 7.9 ***,serial ctDNA detection was used to monitor the efficacy of *** aberration of HER2 copy number disappeared when radiographic assessment revealed a partial ***,a high level of HER2 amplification was detected again at the time of disease ***,a phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha mutation was identified at the time of tumor progression,which may explain the acquired resistance to *** This is the first case report of HER2-positive RAS/BRAF wild-type metastatic CRC patient responding to lapatinib *** highlights that ctDNA testing is an effective and feasible approach to evaluate the efficacy of anti-HER2 therapy.

关键词： Human epidermal growth factor receptor 2 Colorectal cancer Lapatinib Circulating tumor DNA Case report

孕前BMI及孕期BMI变化对产妇分娩方式及围产期结局的影响分析

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中国妇幼健康研究 2024年第10期35卷 85-89页

作者：孙宇王爱华张静范薇杨丽娟罗丹丹昆明市妇幼保健院产科云南昆明650031

目的分析孕前体质指数(BMI)及孕期BMI变化对产妇分娩方式、围产期结局的影响,为备孕期及孕期女性的体重管理提供依据。方法选取2022年8月至2023年8月在昆明市妇幼保健院住院分娩的98例孕妇作为研究对象,按孕前BMI将研究对象分为消瘦组(n... 详细信息

目的分析孕前体质指数(BMI)及孕期BMI变化对产妇分娩方式、围产期结局的影响,为备孕期及孕期女性的体重管理提供依据。方法选取2022年8月至2023年8月在昆明市妇幼保健院住院分娩的98例孕妇作为研究对象,按孕前BMI将研究对象分为消瘦组(n=16)、正常组(n=54)、超重或肥胖组(n=28);按孕期BMI变化分为增重不足组(n=19)、增重正常组(n=27)、增重过多组(n=52),分析组间孕妇分娩方式及围产期结局的差异。结果98例孕妇中,69例行阴道分娩,29例行剖宫产。消瘦组、正常组、超重或肥胖组孕妇分娩方式比较差异具有统计学意义(χ^(2)值为11.068,P0.05);不同孕期BMI变化组别的妊娠并发症、胎儿不良事件、新生儿不良事件及产后并发症比例比较差异均具有统计学意义(χ^(2)值分别为11.651、10.825、16.534、11.159,P<0.05)。结论孕前超重或肥胖、孕期增重过多均可能增加产妇剖宫产及不良围产期结局的发生风险。建议孕前超重或肥胖的妇女在备孕期间进行减重管理,孕期仍需科学管理体重,避免孕期增重过多或增重不足。

关键词：孕妇体重指数分娩方式围产期结局

Study on the Application of Vibration Isolation by Using a Trench in Frame Structures

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Structural Durability & Health Monitoring 2023年第1期17卷 23-36页

作者： Pinwu guan Zheng Ma Pu Zhang Shizhan Xu wei Liu Yanwei Guo Xin Yang Zhen Wang Haibo Wang Chao Yuan Jie Wang Zhengzhou University Zhengzhou450002China Henan Xixi Expressway Construction Co. Ltd.Nanyang450015China

An isolation trench is a simple and effective method to isolate structural vibrations originating from sources of vibration other than earthquakes(machines,traffic,explosions,etc.);however,there is still not a conclusive depth of the isolation trench for frame *** investigate the isolation effect of a trench in the frame structure designed for ground vibration,both a field test and finite element analysis were conducted to analyze the reduced effect of the *** vibration reduction analysis was based on the dynamic equation and wave *** the vibration control of an industrial plant frame,a soil-trench-building finite element model was built to analyze the vibration characteristics of the floor before and after the open isolation trench structure was *** to the model,a dynamic test was carried out on the frame structure to assess the effect of the vibration reduction by introducing the *** results showed that the depth of the trench was the dominating factor in vibration *** the depth of the trench reached 1~1.3 times the wavelength of the Rayleigh wave,the damping effect was the *** width of the trench has little effect on the vibration isolation efficiency,and the trench must be maintained at a certain distance from the building to ensure the vibration damping *** vibration of each floor was obviously reduced after the trench was *** vibration damping effect of the trench was significant.

关键词： Vibration reduction trench finite element process analysis

Research on the Equivalence Between Double Differential- mode Current Injection and Radiation Test Method

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高电压技术 2013年第8期39卷 2031-2037页

作者： PAN Xiaodong wei guanghui fan Lisi LU Xinfu YANG Zhe National Key Laboratory for Electromagnetic Environment Effects Mechanical Engineering College Shijiazhuang 050003 China Radiation Environment Management Center Shijiazhuang 050091 China

There are the application scope limits for single differential-mode current injection test method, so in order to carry out injection susceptibility test for two-pieces equipment interconnected with both ends of a cable simultaneously, a double differential-mode current in- jection test method (DDMCI) is proposed. The method adopted the equivalence source wave theorem and Baum-Liu-Tesche(BLT) equation as its theory foundation. The equivalent corresponding relation between injection voltage and radiation electric field intensity is derived, and the phase relation between the two injection voltage sources is confirmed. The results indicate that the amplitude and phase of the equivalent injection voltage source is closely related to the S parameter of directional coupling device, the transmission line length, and the source vector in BLT equation, but has nothing to do with the reflection coefficient between the two equipment pieces. Therefore, by choosing the right amplitude and phase of the double injection voltage sources, the DDMCI test is equivalent to the radiation test for two interconnected equipment of a system.

关键词：测试方法差模电流辐射测试电流注入等价 BLT方程相位关系敏感性试验

Relationship among the secretion of extracellular polymeric substances, heat resistance, and bioleaching ability of Metallosphaera sedula

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International Journal of Minerals,Metallurgy and Materials 2019年第12期26卷 1504-1511页

作者： Run-lan Yu Zhen-hua Liu Zhao-jing Yu Xue-ling Wu Li Shen Yuan-dong Liu Jiao-kun Li Wen-qing Qin guan-zhou Qiu wei-min Zeng School of Minerals Processing and Bioengineering Central South UniversityChangsha 410083China Key Laboratory of Biometallurgy of Ministry of Education Central South UniversityChangsha 410083China

This paper describes the investigation of the secretion of extracellular polymeric substances(EPS)by an extremely thermoacidophilic archaea,Metallosphaera sedula(***),during the bioleaching of pyrite under different temperatures and discusses the relationship among the EPS secretion,its heat resistance,and its ability to bioleach *** investigation results indicate that the amount of extracellular proteins is significantly higher than the amount of extracellular polysaccharides in the extracted EPS whether free cells or attached cells;these results are quite different from the behavior of mesophilic Acidithiobacillus *** the growth of *** is inhibited at 80℃,the bioleaching ability of *** is only slightly lower than that at the optimum growth temperature of 72℃ because of the heat resistance mechanism based on EPS *** secretion of more extracellular proteins is an important heat resistance mechanism of ***.

关键词： bioleaching extracellular polymeric substances(EPS) heat resistance Metallosphaera sedula

Expression and functional characterization of platelet-derived growth factor receptor-like gene

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World Journal of Gastroenterology 2010年第12期16卷 1465-1472页

作者： Guo, Feng-Jie Zhang, wei-Jia Li, Ya-Lin Liu, Yan Li, Yue-Hui Huang, Jian Wang, Jia-Jia Xie, Ping-Li Li, guan-Cheng Cent S Univ Xiangya Sch Med Canc Res Inst Changsha 410078 Hunan Peoples R China

AIM:To investigate the role of platelet-derived growth factor receptor-like gene(PDGFRL)in the anti-cancer therapy for colorectal cancers(CRC).METHODS:PDGFRL mRNA and protein levels were measured by reverse transcription-polymerase chain reaction(RT-PCR)and immunohistochemistry in CRC and colorectal normal *** prokaryotic expression vector was carried out in Escherichia coli(***),and purified by immobilized metal affinity *** effect of PDGFRL protein on CRC HCT-116 cells was detected by 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide(MTT),clone counting,cell cycle,and wound healing ***:Both RT-PCR and immunohistochemistry showed that the expression of PDGFRL in colorectal normal tissues was higher than in cancer *** pET22b-PDGFRL prokaryotic expression vector was successfully expressed in ***,and the target protein was expressed in the form of inclusion *** purification and refolding,recombinant human PDGFRL(rhPDGFRL)could efficiently inhibit the proliferation and invasion of CRC HCT-116 cells detected by MTT,clone counting and wound healing ***,rhPDGFRL arrested HCT-116 cell cycling at the G0/G1 ***:PDGFRL is a potential gene for application in the anti-cancer therapy for CRC.

关键词： Platelet-derived growth factor receptorlike Colorectal cancer Prokaryotic expression Reverse transcription-polymerase chain reaction Immunohistochemistry