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American Journal of Molecular Biology 2024年第2期14卷 54-65页

作者： Jianzhong Huang Peng Jia Xiaoju Zhong Xiuying guan Hongbin Zhang Honglei ruan Key Laboratory of Chronic Diseases Fuzhou Medical University Fuzhou China

Coding sequences (CDS) are commonly used for transient gene expression, in yeast two-hybrid screening, to verify protein interactions and in prokaryotic gene expression studies. CDS are most commonly obtained using complementary DNA (cDNA) derived from messenger RNA (mRNA) extracted from plant tissues and generated by reverse transcription. However, some CDS are difficult to acquire through this process as they are expressed at extremely low levels or have specific spatial and/or temporal expression patterns in vivo. These challenges require the development of alternative CDS cloning technologies. In this study, we found that the genomic intron-containing gene coding sequences (gDNA) from Arabidopsis thaliana, Oryza sativa, Brassica napus, and Glycine max can be correctly transcribed and spliced into mRNA in Nicotiana benthamiana. In contrast, gDNAs from Triticum aestivum and Sorghum bicolor did not function correctly. In transient expression experiments, the target DNA sequence is driven by a constitutive promoter. Theoretically, a sufficient amount of mRNA can be extracted from the N. benthamiana leaves, making it conducive to the cloning of CDS target genes. Our data demonstrate that N. benthamiana can be used as an effective host for the cloning CDS of plant genes.

关键词： Coding Sequence Genomic Sequence Nicotiana benthamiana Plant Genes

Versatile application of wet-oxidation for ambient CO abatement over Fe(OH)_x supported subnanometer platinum group metal catalysts

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Chinese Journal of Catalysis 2020年第4期41卷 613-621页

作者： Hongling guan Yang Chen Chongyan ruan Jian Lin Yang Su Xiaodong Wang Lingbo Qu School of Chemical Engineering Zhengzhou UniversityZhengzhou 450001HenanChina CAS Key Laboratory of Science and Technology on Applied Catalysis Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023LiaoningChina College of Chemistry Zhengzhou UniversityZhengzhou 450001HenanChina

The efficient and stable abatement of CO pollutant under ambient conditions is of great significance;however,it remains a formidable ***,we report the versatile application of wet oxidation over Fe(OH)x supported subnanometer Pt group metal(PGM)catalysts for the complete removal of CO under ambient temperature and humidity ***,the 1.8 wt%Rh/Fe(OH)x catalyst exhibited better durability during a^1400 min run for wet oxidation than for dry CO *** characterization results including HR-TEM,H2-TPR,and in-situ DRIFTS suggested that Fe(OH)x,with good reducibility,promoted by the subnanometer Rh clusters,provided sites for the adsorption and reaction of O2 and H2 O to form OH ***,these OH species reacted with the adsorbed CO on Rh sites with a considerably lower activation energy(9 kJ mol^-1)than that of dissociated 0 species(22 kJ mol^-1),thus rationalizing the outstanding performance of Rh/Fe(OH)x for wet *** experiments with other PGMs revealed a good generality for the application of wet oxidation in the efficient abatement of CO under humid conditions with Fe(OH)x as the support.

关键词： Platinum group metals CO Oxidation Ambient condition Fe(OH)x Subnanometer clusters

Genomic prediction of the performance of hybrids and the combining abilities for line by tester trials in maize

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The Crop Journal 2022年第1期10卷 109-116页

作者： Ao Zhang Paulino Pérez-Rodríguez Felix San Vicente Natalia Palacios-Rojas Thanda Dhliwayo Yubo Liu Zhenhai Cui Yuan guan Hui Wang Hongjian Zheng Michael Olsen Boddupalli M.Prasanna Yanye ruan Jose Crossa Xuecai Zhang College of Biological Science and Technology Shenyang Agricultural UniversityShenyang 110866LiaoningChina b International Maize and Wheat Improvement Center(CIMMYT) El BatanTexcoco 56237Mexico CIMMYT-China Specialty Maize Research Center Shanghai Academy of Agricultural SciencesShanghai 200063China Colegio de Postgraduados Estado De MéxicoMexico Crop Breeding and Cultivation Research Institute Shanghai Academy of Agricultural SciencesShanghai 200063China International Maize and Wheat Improvement Center(CIMMYT) P.O.Box 1041Village MarketNairobi 00621Kenya

The two most important activities in maize breeding are the development of inbred lines with high values of general combining ability(GCA)and specific combining ability(SCA),and the identification of hybrids with high yield *** selection(GS)is a promising genomic tool to perform selection on the untested breeding material based on the genomic estimated breeding values estimated from the genomic prediction(GP).In this study,GP analyses were carried out to estimate the performance of hybrids,GCA,and SCA for grain yield(GY)in three maize line-by-tester trials,where all the material was phenotyped in 10 to 11 multiple-location trials and genotyped with a mid-density molecular marker *** showed that the prediction abilities for the performance of hybrids ranged from 0.59 to0.81 across all trials in the model including the additive effect of lines and *** the model including both additive and non-additive effects,the prediction abilities for the performance of hybrids were improved and ranged from 0.64 to 0.86 across all *** prediction abilities of the GCA for GY were low,ranging between-0.14 and 0.13 across all trials in the model including only inbred lines;the prediction abilities of the GCA for GY were improved and ranged from 0.49 to 0.55 across all trials in the model including both inbred lines and testers,while the prediction abilities of the SCA for GY were negative across all *** prediction abilities for GY between testers varied from-0.66 to 0.82;the performance of hybrids between testers is difficult to *** offers the opportunity to predict the performance of new hybrids and the GCA of new inbred lines based on the molecular marker information,the total breeding cost could be reduced dramatically by phenotyping fewer multiple-location trials.

关键词： Maize Genomic selection Line-By-Tester General combining ability Specific combining ability

基于Hausdorff距离的非刚体目标自适应轮廓跟踪

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通信学报 1998年第11期19卷 38-43页

作者：关海英阮秋琦北方交通大学信息科学研究所

本文提出了一种基于Ｈａｕｓｄｏｒｆ距离的非刚体目标的轮廓跟踪算法。它的特点在于从二维序列图像中提取非刚体目标的二维可变模板，从而实现对非刚体运动目标的跟踪。此算法的主要思想在于将空间运动的非刚体目标的二维图像变化分解... 详细信息

本文提出了一种基于Ｈａｕｓｄｏｒｆ距离的非刚体目标的轮廓跟踪算法。它的特点在于从二维序列图像中提取非刚体目标的二维可变模板，从而实现对非刚体运动目标的跟踪。此算法的主要思想在于将空间运动的非刚体目标的二维图像变化分解成二维运动变化和二维形状变化，从而加大了模板适应力，辅以过零点检测及金字塔快速搜索算法，可以实现非刚体目标的快速跟踪。最后，本文给出了对人体轮廓进行跟踪的实验结果。

关键词：金字塔算法目标跟踪图像序列分析计算机视觉

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木质材料中动弹模量色散关系的分析

南京林业大学学报（自然科学版） 2007年第1期31卷 131-134页

作者：余观夏阮锡根南京林业大学信息科学技术学院江苏南京210037

用应力波的方法建立一维木材动弹模量的色散关系方程,并测量和分析了两种木质材料的动弹模量与频率的色散关系。在纵振动过程中,两种木材的动弹模量随着频率的增大而增大,而耗散因子随着频率的增大而减少。

关键词：木质材料动弹模量耗散因子色散应力波

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数字借阅补偿金制度及其对我国的启示

国家图书馆学刊 2024年第3期33卷 3-12页

作者：阮开欣官洛羽华东政法大学知识产权学院

公共借阅权有排他权、获酬权和补偿金三种实施模式,其中补偿金模式是当前各国的主流选择。数字时代,很多国家开始探索将公共借阅权拓展至数字领域,澳大利亚、加拿大、英国和丹麦四国均已建立起数字借阅补偿金制度。这一制度符合功利主... 详细信息

公共借阅权有排他权、获酬权和补偿金三种实施模式,其中补偿金模式是当前各国的主流选择。数字时代,很多国家开始探索将公共借阅权拓展至数字领域,澳大利亚、加拿大、英国和丹麦四国均已建立起数字借阅补偿金制度。这一制度符合功利主义社会整体利益最大化的理念。在目前制度实践的基础上,本文总结了数字借阅补偿金的运行机制,主要体现在受益者范围、借阅模式、报酬支付和管理体系四个方面。我国应当引入数字借阅补偿金制度,以激励文化创新,促进知识传播,从而实现社会利益最大化。

关键词：公共借阅权公平补偿数字阅读

Numerical Simulation and Dynamical Analysis for Low Salinity Water Lens in the Expansion Area of the Changjiang Diluted Water

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China Ocean Engineering 2014年第6期28卷 777-790页

作者：张文静朱首贤李训强阮鲲管卫兵彭剑 College of Meteorology and Oceanography PLA University of Science and Technology College of Harbor Coastal and Offshore Engineering Hohai University State Key Laboratory of Satellite Ocean Environment Dynamics Second Institute of Oceanography

The low salinity water lenses（LSWLes） in the expansion area of the Changjiang diluted water（CDW） exist in a certain period of time in some years. The impact of realistic river runoff, ocean currents and weather conditions need to be taken into account in the dynamical analysis of LSWL, which is in need of research. In this paper, the POM-σ-z model is used to set up the numerical model for the expansion of the CDW. Then LSWL in summer 1977 is simulated, and its dynamic mechanism driven by wind, tide, river runoff and the Taiwan Warm Current is also analyzed. The simulated results indicate that the isolated LSWL detaches itself from the CDW near the river mouth, and then moves towards the northeast region outside the Changjiang Estuary. Its maintaining period is from July 26 to August 11. Its formation and development is mainly driven by two factors. One is the strong southeasterly wind lasting for ten days. The other is the vertical tidal mixing during the transition from neap tide to spring tide.

关键词： Changjiang diluted water low salinity water lens numerical simulation dynamic mechanism

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基于有限元模拟的极限拉深系数优化

塑性工程学报 2006年第1期13卷 32-35页

作者：黄珍媛阮锋管灿华南理工大学机械学院模具研究室广州510640 杭州卓越机械设备有限公司广州分公司广州510060

结合0.618优化算法,运用ANSYS分析软件,通过改变坯料尺寸的方法,对板料的成形过程进行模拟,对板材的极限拉深系数进行逼近优化,并将模拟和优化结果与物理拉深试验结果进行了比较。

关键词： 0.618优化算法 ANSYS 模拟极限拉深系数优化

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基于双路径特征融合的轻量级脑肿瘤分割网络

天津大学学报（自然科学与工程技术版） 2024年第11期57卷 1177-1186页

作者：李锵阮方号关欣天津大学微电子学院天津300072

脑肿瘤是世界上最致命的癌症之一,从三维核磁共振图像(MRI)中快速、准确地分割正常脑组织和恶性肿瘤组织,对临床诊断和手术治疗都至关重要.近年来,基于卷积神经网络的分割架构,特别是3D U-Net架构在脑肿瘤分割方面取得了巨大成功.然而,... 详细信息

脑肿瘤是世界上最致命的癌症之一,从三维核磁共振图像(MRI)中快速、准确地分割正常脑组织和恶性肿瘤组织,对临床诊断和手术治疗都至关重要.近年来,基于卷积神经网络的分割架构,特别是3D U-Net架构在脑肿瘤分割方面取得了巨大成功.然而,现有的基于3D U-Net的网络存在下采样信息丢失的问题,缺乏自动聚焦小尺度病灶的能力,编码器和解码器之间的全局上下文信息交互不够充分,并且参数量大、计算开销高.针对上述问题,提出一种双路径特征融合的轻量级脑肿瘤分割网络.首先,该网络通过增加一条分支路径,将原始数据中的低级细节信息添加到编码器的各层中,弥补下采样带来的特征信息损失.其次,提出一种多层金字塔制导模块代替传统的跳跃连接,增强解码器不同层次的全局上下文信息获取能力.最后,在输出层引入多视图级联注意力模块,利用肿瘤不同区域的包含关系,使网络从各个视图自动聚焦小尺度肿瘤区域.在BraTS2020数据集上的实验结果表明,采用0.55×10^(6)参数量和41.21×10^(9)浮点运算次数,该网络在增强肿瘤、全肿瘤和肿瘤核心的Dice系数分别可达78.64%、89.51%和83.77%.此外,在BraTS2018数据集上进一步评估该网络的性能,实验结果表明:该网络在保持较小计算量的同时,显著提高了对脑肿瘤各病灶区域的定位能力和分割性能,在临床实践中具有重要意义.

关键词：核磁共振图像脑肿瘤分割轻量级双路径多视图级联多层金字塔制导

间歇采样重复转发式干扰对CPM-LFM信号干扰效果分析

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无线电工程 2022年第8期52卷 1395-1401页

作者：王冠阮嘉恒空军通信士官学校综合训练系辽宁大连116600 中国电子科技集团公司第五十四研究所河北石家庄050081

针对传统干扰方式无法有效干扰新体制雷达信号的问题,提出采用目前雷达主流干扰方式——间歇采样重复转发式干扰(Intermittent Sampling Repeat Forwarding Jamming,ISRJ),对连续相位调制—线性调频(Continuous Phase Modulation-Linear... 详细信息

针对传统干扰方式无法有效干扰新体制雷达信号的问题,提出采用目前雷达主流干扰方式——间歇采样重复转发式干扰(Intermittent Sampling Repeat Forwarding Jamming,ISRJ),对连续相位调制—线性调频(Continuous Phase Modulation-Linear Frequency Modulation,CPM-LFM)信号进行干扰效果分析。建立了CPM-LFM雷达通信一体化信号模型。推导干扰信号的脉冲压缩表达式,分析了ISRJ脉冲压缩结果。从实际应用角度出发,与传统干扰方式对比说明ISRJ方式在一体化信号干扰上的优势。仿真结果表明,ISRJ能够有效干扰CPM-LFM一体化信号,影响雷达探测能力和通信性能,在干信比(Jamming Signal Ratio,JSR)为30 dB时,通信误码率为0.1938,实现中度干扰。

关键词：连续相位调制一体化信号脉冲压缩间歇采样转发式干扰