检索结果-南通市图书馆

Ultrastructure and phylogeny of Ustilago coicis

在线全文

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第4期14卷 336-345页

作者： Jing-ze ZHANG Pei-gang guan gang TAO Mohammad Reza OJAGHIAN Kevin David HYDE Institute of Biotechnology College of Agriculture and BiotechnologyZhejiang University Guizhou Institute of Plant Protection Key Laboratory of Systematic Mycology and Lichenology Institute of MicrobiologyChinese Academy of Sciences Institutue of Excellence in Fungal Research School of ScienceMae Fah Luang University

Ustilago coicis causes serious smut on Coix lacryma-jobi in Dayang Town,Jinyun County,Zhejiang Province of *** this paper,ultrastructural assessments on fungus-host interactions and teliospore development are presented,and molecular phylogenetic analyses have been done to elucidate the phylogenetic placement of the *** growth within infected tissues was both intracellular and intercellular and on the surface of fungus-host interaction,and the fungal cell wall and the invaginated host plasma membrane were separated by a sheath comprising two distinct layers between the fungal cell wall and the invaginated host plasma *** development of teliospore walls was unique as they appeared to be originated from the *** addition,internal transcribed spacer(ITS) and large subunit(LSU) sequence data showed that *** is closely related to Ustilago trichophora which infects grass species of the genus Echinochloa(Poaceae).

关键词： Fungus-host interaction Molecular phylogenetics Smut Teliospore wall development

Microstructure and corrosion properties of Mg-0.5Zn-0.2Ca-0.2Ce alloy with different processing conditions

在线全文

学校读者我要写书评

暂无评论

Rare Metals 2021年第7期40卷 1924-1931页

作者： Cheng Zhang Liang Wu guang-Sheng Huang guan-gang Wang Bin Jiang Fu-Sheng Pan State Key Laboratory of Mechanical Transmission College of Materials Science and EngineeringChongqing UniversityChongqing 400044China

The microstructure and corrosion resistance of Mg-0.5 Zn-0.2 Ca-0.2 Ce alloy with different processing conditions were *** composition was detected by X-ray fluorescence(XRF),and the microstructure was analyzed by optical microscopy(OM)and scanning electron microscope(SEM)equipped with energy-dispersive spectroscopy(EDS).The corrosion behavior was investigated by hydrogen evolution tests,weight loss tests and electrochemical *** Mg-0.5 Zn-0.2 Ca-0.2 Ce alloy has much better corrosion resistance compared with the commercial AZ31 sheet,which can be attributed to its dispersive second phases and protective corrosion products film on the alloy ***,the as-rolled Mg-0.5 Zn-0.2 Ca-0.2 Ce alloy shows much better corrosion resistance compared with the as-extruded Mg-0.5 Zn-0.2 Ca-0.2 Ce *** can be due to three aspects:The as-rolled alloy has smaller grain size;the as-rolled alloy has lower(1010)/(1120)texture intensity;the residual stress of the as-rolled alloy is eliminated during the annealing process,but large residual stress exists in the asextruded alloy produced by the extrusion process.

关键词： Mg-0.5Zn-0.2Ca-0.2Ce alloy Processing conditions Texture Corrosion behavior

Influence of Grain Orientation on the Corrosion Behavior of Rolled AZ31 Magnesium Alloy

在线全文

学校读者我要写书评

暂无评论

Acta Metallurgica Sinica(English Letters) 2015年第11期28卷 1387-1393页

作者： Yan-Chun Zhao guang-Sheng Huang guan-gang Wang Ting-Zhuang Han Fu-Sheng Pan State Key Laboratory of Mechanical Transmission College of Materials Science and EngineeringChongqing University National Engineering Research Center for Magnesium Alloys Chongqing University Chongqing Research Center for Advanced Materials Chongqing Academy of Science and Technology

The effect of the grain orientation on corrosion behavior of rolled AZ31 magnesium alloy is investigated in this study. The test samples have a similar surface roughness to the Mg alloy in practical application. The immersion test and electrochemical impedance spectroscopy show that the TD-ND planes dominated by （101-0）, （1120） and （1011） oriented grains show a higher corrosion resistance compared with these of the RD-TD planes which consist mainly of （0001） oriented grains. Here, RD, ND and TD represent the rolling direction, the normal direction and the transverse direction of the alloy sheet, respectively. The surface morphologies of the alloys at various immersion stages are observed by scanning electron microscopy, and the surface topography of the alloy substitutes is also observed by laser scanning confocal microscopy. The TD-ND planes show a regular corrosion along the TD direction, but the RD-TD plane shows an irregular corrosion.

关键词： Magnesium alloys Corrosion behavior Electrochemical impedance spectroscopy Crystallographic orientation

Influence of Continuous Bending Process on Texture Evolution and Mechanical Properties of AZ31 Magnesium Alloy

在线全文

学校读者我要写书评

暂无评论

Acta Metallurgica Sinica(English Letters) 2018年第3期31卷 225-233页

作者： Ting-Zhuang Han guang-Sheng Huang Lun Huang Bin Jiang guan-gang Wang Ai-Tao Tang Fu-Sheng Pan State Key Laboratory of Mechanical Transmission College of Materials Science and Engineering Chongqing University National Engineering Research Center for Magnesium Alloys Chongqing University Chongqing Research Center for Advanced Materials Chongqing Academy of Science & Technology

Mg-3Al-lZn alloy sheets have been deformed by continuous bending（CB）to investigate the effects of pro-cessing parameters（bending angle and repetitive passes）on the texture and *** samples exhibited a bimodal microstructure with abnormal growth grains in the surface region and fine grains distributed in the center after CB process followed by *** texture evolution measured by XRD indicated that the basal poles were rotated from ND toward RD,and the texture intensity decreased with the bending angle decreasing and repetitive passes *** with the as-received sample,the yield strength of CBA-120-2 sample significantly decreased from 183 to 112 MPa,and a smaller r-value and a larger n-value were *** formability of CB processed samples in annealing condition was significantly enhanced with the highest of Erichsen value of 5.4 mm,increased by about 135%.The improvement of formability was likely attributed to the weakened and RD-tilt basal texture and coarse grains in the surface part.

关键词： Magnesium alloy Continuous bending Microstructure Texture Stretch formability

New Characteristics of Residential Housing Construction Quality in China

在线全文

学校读者我要写书评

暂无评论

平顶山工学院学报 2008年第2期17卷 41-44页

作者： guan gang SUN gang-zhu （ Xi＇an University of Architechure and Technology College of Civil Engineering Zhengzhou University 450002 China ）

Base on the investigation results of residential housing construction quality recent years in China,this research first analyzes the general quality condition of residential housing construction,then demonstrates the quality condition in different regions in our country,finds out main quality problems in the residential housing construction and studies the new characteristics of quality problems,finally provides the construction industry positive foundation to solve the quality problems objectively for residential housing construction.

关键词：中国建筑物建筑质量质量管理

GST Fusion Protein Based Specific Polyclonal Antibody Preparation of Mouse Aquaporin 1

在线全文

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2009年第4期25卷 500-505页

作者： LI Jiang YANG Nan-yang guan Xin-gang ZHANG Shu-zhi ZHANG Yan QIN Mei-ling MA Tong-hui LI Xiao-meng Dental Hospital Jilin University Changchun 130041 P. R. China Membrane Channel Research Laboratory School of Life Sciences Northeast Normal University Changchun 130024 P. R. China

Aquaporins（AQPs） are specific membrane channels for water and other small nonionic *** order to overcome the difficulties to generate the effictive antibody of membrane protein,we selected the cytoplasmic C-terminus of Aquaporin 1（AQP1） as an unique *** long C-terminus of mouse AQP1 was overexpressed in the Glutathione S-tansferase Gene Fusion *** the basis of the resonable amounts of soluable membrane protein peptides,we prepared the specific *** pursure this object,we constructed pGEX-4T-1/mAQP1（DNA sequence from 700 to 801 bp） recombinant plasmid and transformed it into Escherichia coli BL21 *** GST-AQP1 C-terminal hydrophilic peptide fusion protein was induced by IPTG and further purified by Glutathione Sepharose 4B to obtain the right size fusion *** we immunized the New Zealand rabbits to prepare the *** purified AQP1 antibody showed high sensitivity by ELISA assay and high specificity by Western blot with AQP1 null mice served as negative ***,we also checked the AQP1 localization in the mouse renal tissues in wild type of mice and AQP1 null mice served as negative *** demonstrated that AQP1 was highly expressed at the descending limb of Henle tube using our purified AQP1 antibody,which was consistent with previous *** successful design and preparation of AQP1 antibody through GST technique is an example as making antibodies against a specific membrane protein.

关键词： Aquaporin 1 GST fusion protein Polyclonal antibody Gene knockout mice Membrane protein

Altered Taurine Release in Hypothalamus of Aquaporin-4 Knockout Mice

在线全文

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2010年第3期26卷 406-408页

作者： ZHANG Hong-guo XIAO Ying-hong YANG Long-fei SU Wei-heng guan Xin-gang WANG Yu FENG Xue-chao MA Tong-hui Membrane Channel Research Laboratory Northeast Normal University Changchun 130024 P. R. China

Taurine is concentrated in glial cells in the hypothalamus and released in an osmo-dependent manner through volume-sensitive anion channels. Released tanrine acts on glycine receptors on vasopressin neurons to control vasopressin secretion. Water channel AQP4 is abundant in astrocytes in osmosensory areas such as the supraoptic nucleus of hypothalamus. An HPLC-based method was established to quantify taurine release from isolated hypothalamus tissues in wildtype and AQP4 knockout mice. Under the basal condition, there was no difference in taurine release from AQP4^＋/＋ and AQP4^-/- hypothalamuses. Taurine release from AQP4^-/- hypothalamus under hypoosmotic stimulation was significantly lower than that from AQP4^＋/＋ mice. AQP4 expression in the glial cells of the hypothalamus may play an important role in osmoregulation oftaurine release and subsequent vasopressin secretion.

关键词： Aquaporin-4 Taurine Hypothalamus HPLC Precolumn derivatization

Establishment of a simple assay in vitro for hepatitis C virus NS3 serine protease based on recombinant substrate and single-chain protease

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2002年第6期8卷 1088-1093页

作者： Gui-Xin Du Li-Hua Hou Rong-Bin guan Yi-gang Tong Hai-Tao Wang,Department of Applied Molecular Biology,Institute of Microbiology and Epidemiology,Fengtai,Beijing 100071,China Department of Applied Molecular Biology Institute of Microbiology and Epidemiology Fengtai Beijing 100071 China. dugx@***

AIM: To establish a simple and convenient assay in vitro for the Hepatitis C virus NS3 serine protease based on the recombinant protease and substrate, and to evaluate its feasibility in screening the enzyme inhibitors. METHODS: Based on the crystallographic structure of hepatitis C virus (HCV) serine protease, a novel single-chain serine protease was designed, in which the central sequence of cofactor NS4A was linked to the N-terminus of NS3 serine protease domain via a flexible linker GSGS. The fusion gene was obtained by two-step PCR that was carried out with three primers and then cloned into the prokaryotic expression vector pQE30, and the recombinant clone was verified by DNA sequencing. The single-chain recombinant protease was expressed when the *** induced with IPTG and the expression conditions were optimized to produce large amount of soluble protease. The recombinant substrate NS5ab that covers the cleavage point NS5A/B was also expressed in ***. Both of the protease and substrate were purified by using Ni-NTA agarose metal affinity resin, then they were mixed together in a specific buffer, and the mixture was analyzed by SDS-PAGE. The cleavage system was used to evaluate some compounds for their inhibitory activity on serine ***: The single-chain recombinant protease was overexpressed as soluble protein when the E. coliwas induced at a low dosage of IPTG (0.2 mM) and cultured at a low temperature (15℃). The protease was purified by using Ni-NTA agarose metal affinity resin (the purity is over 95 %).The recombinant substrate NS5ab was expressed in an insoluble form and could refold successfully after purification and dialysis. A simple and convenient assay in vitro was established, in which the purified single-chain serine protease could cleave the recombinant substrate NS5ab into two fragments that were visualized by SDS-PAGE. PMSF had an effect on inhibiting activity of serine protease, while EDTA had ***: A simple and

关键词： C肝病毒 NS3丝氨酸蛋白酶体外简易检验法重组基质单链蛋白酶

In vitro assay for HCV serine proteinase expressed in insect cells

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2003年第7期9卷 1629-1632页

作者： Li-Hua Hou Gui-Xin Du Rong-Bin guan Yi-gang Tong Hai-Tao Wang, Department of Applied Molecular Biology, Institute of Microbiology and Epidemiology, Beijing 100071, China Department of Applied Molecular Biology Institute of Microbiology and Epidemiology Beijing 100071 China. houlihua@***

AIM: To produce the recombinant NS3 protease of hepatitis C virus with enzymatic activity in insect ***: The gene of HCV serine proteinase domain which encodes 181 amino acids was inserted into pFastBacHTc and the recombinant plasmid pFBCNS3N was transformed into DH10Bac competent cells for *** the recombinant bacmids had been determined to be correct by both blue-white colonies and PCR analysis, the isolated bacmid DNAs were transfected into Sf9 insect *** bacmids DNA was verified to replicate in insect cells and packaged into baculovirus particles via PCR and electronic microscopic analysis. The insect cells infected with recombinant baculovirus were determined by SDS-PAGE and Western-blot assays. The recombinant protein was soluted in N-lauryl sarcosine sodium (NLS) and purifed by metalchelated-affinity chromatography, then the antigenicity of recombinant protease was determined by enzyme-linked immunoabsorbant assay and its enzymatic activity was ***: The HCV NS3 protease domain was expressed in insect cells at high level and it was partially solved in *** 0.2 mg recombinant serine proteinase domain with high purity was obtained by metal-chelated-affinity chromatography from 5×107 cells, and both antigenicity and specificity of the protein were evaluated to be high when used as antigen to detect hepatitis C patients′ sera in indirect ELISA format. In vitro cleavage assay corroborated its enzymatic ***: The recombinant HCV NS3 proteinase expressed by insect cells is a membrane-binding protein with good antigenicity and enzymatic activity.

关键词：丙型肝炎病毒丝氨酸蛋白酶基因重组聚合酶链反应