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Synthetic Gene Circuit-Based Assay with Multilevel Switch Enables Background-Free and Absolute Quantification of Circulating Tumor DNA

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Research 2023年第4期2023卷 821-832页

作者： Chao Zhang Zedong Li Jie Liu Chang Liu Haoqing Zhang Won gu Lee Chunyan Yao Hui guo feng xu The Key Laboratory of Biomedical Information Engineering of Ministry of Education School of Life Science and TechnologyXi’an Jiaotong UniversityXi’an 710049P.R.China Bioinspired Engineering and Biomechanics Center(BEBC) Xi'an Jiaotong UniversityXi’an 710049P.R.China TFX GroupXi'an Jiaotong University Institute of Life Health Xi'an 710049P.R.China Department of Mechanical Engineering Kyung Hee UniversityYongin 17104Republic of Korea Department of Transfusion Medicine Southwest HospitalThird Military Medical University(Army Medical University)Chongqing 400038P.R.China Department of Medical Oncology The First Affiliated Hospital of Xi'an Jiaotong UniversityXi'an 710061P.R.China

Circulating tumor DNA(ctDNA)detection has found widespread applications in tumor diagnostics and treatment,where the key is to obtain accurate quantification of ***,this remains challenging due to the issue of background noise associated with existing *** this work,we developed a synthetic gene circuit-based assay with multilevel switch(termed CATCH)for background-free and absolute quantification of *** multilevel switch combining a small transcription activating RNA and a toehold switch was designed to simultaneously regulate transcription and translation processes in gene circuit to eliminate background ***,such a multilevel switch-based gene circuit was integrated with a Cas9 nickase H840A(Cas9n)recognizer and a molecular beacon reporter to form CATCH for ctDNA *** CATCH can be implemented in one-pot reaction at 35°C with virtually no background noise,and achieve robust absolute quantification of ctDNA when integrated with a digital chip(i.e.,digital CATCH).Finally,we validated the clinical capability of CATCH by detecting drug-resistant ctDNA mutations from the plasma of 76 non-small cell lung cancer(NSCLC)patients,showing satisfying clinical sensitivity and *** envision that the simple and robust CATCH would be a powerful tool for next-generation ctDNA detection.

关键词： Circulating specificity absolute

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Microbiologically influenced corrosion behavior of S32654 super austenitic stainless steel in the presence of marine Pseudomonas aeruginosa biofilm

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Journal of Materials Science & Technology 2017年第12期33卷 1596-1603页

作者： Huabing Li Chuntian Yang Enze Zhou Chunguang Yang Hao feng Zhouhua Jiang Dake xu Tingyue gu Ke Yang School of Metallurgy Northeastern University Institute of Metal Research Chinese Academy of Sciences Department of Chemical and Biomolecular Engineering Institute for Corrosion and Multiphase Technology Ohio University

S32654 super austenitic stainless steel（SASS） is widely used in highly corrosive environments. However,its microbiologically influenced corrosion（MIC） behavior has not been reported yet. In this study, the corrosion behavior of S32654 SASS caused by a corrosive marine bacterium Pseudomonas aeruginosa was investigated using electrochemical measurements and surface analysis techniques. It was found that P. aeruginosa biofilm accelerated the corrosion rate of S325654 SASS, which was demonstrated by a negative shift of the open circuit potential（EOCP）, a decrease of polarization resistance and an increase of corrosion current density in the culture medium. The largest pit depth of the coupons exposed in the *** broth for 14 days was 2.83 m, much deeper than that of the control（1.33 m） in the abiotic culture medium. It was likely that the P. aeruginosa biofilm catalyzed the formation of CrO_3, which was detrimental to the passive film, resulting in MIC pitting corrosion.

关键词： Microbiologically influenced corrosion Super austenitic stainless steel Pseudomonas aeruginosa Biofilm Pitting corrosion

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Parameterization Transfer for a Planar Computational Domain in Isogeometric Analysis

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Computer Modeling in Engineering & Sciences 2023年第11期137卷 1957-1973页

作者： Jinlan xu Shuxin Xiao Gang xu Renshu gu School of Computer Science and Technology Hangzhou Dianzi UniversityHangzhou310018China

In this paper,we propose a parameterization transfer algorithm for planar domains bounded by B-spline curves,where the shapes of the planar domains are *** domain geometries are considered to be similar if their simplified skeletons have the same *** domain we call source domain,and it is parameterized using multi-patch B-spline *** resulting parameterization is C1 continuous in the regular region and G1 continuous around singular points regardless of whether the parameterization of the source domain is C1/G1 continuous or *** this algorithm,boundary control points of the source domain are extracted from its parameterization as sequential points,and we establish a correspondence between sequential boundary control points of the source domain and the target boundary through discrete sampling and *** of the parametrization satisfies C1/G1 continuity under discrete harmonic mapping with continuous *** new algorithm has a lower calculation cost than a decomposition-based parameterization with a high-quality parameterization *** demonstrate that the result of the parameterization transfer in this paper can be applied in isogeometric ***,because of the consistency of the parameterization for the two models,this method can be applied in many other geometry processing algorithms,such as morphing and deformation.

关键词： Isogeometric analysis parameterization transfer discrete harmonic mapping C1/G1 continuity

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A novel method for AFRPs burrs removal:Principle of mechanochemo-induced fiber fracture

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Chinese Journal of Aeronautics 2024年第7期37卷 522-538页

作者： Jie xu Bo LI Pingfa feng Qiang WANG feng feng Division of Advanced Manufacturing Tsinghua Shenzhen International Graduate SchoolTsinghua UniversityShenzhen 518055China Department of Mechanical Engineering Tsinghua UniversityBeijing 100084China Changhe Aircraft Industry(Group)Co.Ltd Jingdezhen 333000China

Burrs generated during the machining of Aramid-Fiber-Reinforced Composites(AFRPs)pose a challenge for the production efficiency of aircraft and helicopter housing *** studies have generally attempted to suppress burrs by referring to delamination suppression *** contrast to stratification,burrs are remediable machining *** such,a mechanochemical method with burrs trimming technological strategy are implemented to effectively combat ***,we clarify the mechanism by which aramid fibers cannot be cut off using analytical and numerical *** addition,the mechanism of fiber fracture with Modified Polyurethane Reactive Polymer(M-PUR),and development of anti-burr devices(thermostatic adhesive sealed generator)are ***,the experimental results show that the reduction rate in burr length is 87%-91%through the mechanochemical *** method not only opens a new avenue to solve the burr problem of aramid fibers but also builds an interdisciplinary bridge between polymer science and composite machining.

关键词： Mechanochemical method Burrs Orthogonal cutting Aramid fiber-reinforced composites Milling

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Non-coding RNAs in acute ischemic stroke:from brain to periphery

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Neural Regeneration Research 2025年第1期20卷 116-129页

作者： Shuo Li Zhaohan xu Shiyao Zhang Huiling Sun Xiaodan Qin Lin Zhu Teng Jiang Junshan Zhou Fuling Yan Qiwen Deng Department of Neurology Nanjing First HospitalNanjing Medical UniversityNanjingJiangsu ProvinceChina Department of Neurology Zhongda HospitalSchool of MedicineSoutheast UniversityNanjingJiangsu ProvinceChina General Clinical Research Center Nanjing First HospitalNanjing Medical UniversityNanjingJiangsu ProvinceChina

Acute ischemic stroke is a clinical emergency and a condition with high morbidity,mortality,and *** predictive,diagnostic,and prognostic biomarkers and effective therapeutic targets for acute ischemic stroke remain *** innovations in high-throughput gene sequencing analysis,many aberrantly expressed non-coding RNAs(ncRNAs)in the brain and peripheral blood after acute ischemic stroke have been found in clinical samples and experimental *** expressed ncRNAs in the post-stroke brain were demonstrated to play vital roles in pathological processes,leading to neuroprotection or deterioration,thus ncRNAs can serve as therapeutic targets in acute ischemic ***,distinctly expressed ncRNAs in the peripheral blood can be used as biomarkers for acute ischemic stroke prediction,diagnosis,and *** particular,ncRNAs in peripheral immune cells were recently shown to be involved in the peripheral and brain immune response after acute ischemic *** this review,we consolidate the latest progress of research into the roles of ncRNAs(microRNAs,long ncRNAs,and circular RNAs)in the pathological processes of acute ischemic stroke–induced brain damage,as well as the potential of these ncRNAs to act as biomarkers for acute ischemic stroke prediction,diagnosis,and *** from this review will provide novel ideas for the clinical application of ncRNAs in acute ischemic stroke.

关键词： acute ischemic stroke apoptosis blood–brain barrier damage circular RNAs excitatory toxicity long non-coding RNAs microRNAs neuroinflammation non-coding RNAs oxidative stress

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Müller cells are activated in response to retinal outer nuclear layer degeneration in rats subjected to simulated weightlessness conditions

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Neural Regeneration Research 2025年第7期20卷 2116-2128页

作者： Yuxue Mu Ning Zhang Dongyu Wei guoqing Yang Lilingxuan Yao Xinyue xu Yang Li Junhui xue Zuoming Zhang Tao Chen Aerospace Clinical Medical Center School of Aerospace MedicineAir Force Medical UniversityXi’anShaanxi ProvinceChina Department of Aviation Medicine Xijing HospitalAir Force Medical UniversityXi’anShaanxi ProvinceChina Department of Emergency Medicine Wuhan No.1 HospitalWuhanHubei ProvinceChina Third Regiment School of Basic MedicineAir Force Medical UniversityXi’anShaanxi ProvinceChina Fourth Regiment School of Basic MedicineAir Force Medical UniversityXi’anShaanxi ProvinceChina

A microgravity environment has been shown to cause ocular damage and affect visual acuity,but the underlying mechanisms remain ***,we established an animal model of weightlessness via tail suspension to examine the pathological changes and molecular mechanisms of retinal damage under *** 4 weeks of tail suspension,there were no notable alterations in retinal function and morphology,while after 8 weeks of tail suspension,significant reductions in retinal function were observed,and the outer nuclear layer was thinner,with abundant apoptotic *** investigate the mechanism underlying the degenerative changes that occurred in the outer nuclear layer of the retina,proteomics was used to analyze differentially expressed proteins in rat retinas after 8 weeks of tail *** results showed that the expression levels of fibroblast growth factor 2(also known as basic fibroblast growth factor)and glial fibrillary acidic protein,which are closely related to Müller cell activation,were significantly *** addition,Müller cell regeneration and Müller cell gliosis were observed after 4 and 8 weeks,respectively,of simulated *** findings indicate that Müller cells play an important regulatory role in retinal outer nuclear layer degeneration during weightlessness.

关键词： glial fibrous acidic protein gliosis Müller cells nerve growth factor neural differentiation neurodegeneration proteomic retinal degeneration retinal outer nuclear layer simulated weightlessness

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AAV mediated carboxyl terminus of Hsp70 interacting protein overexpression mitigates the cognitive and pathological phenotypes of APP/PS1 mice

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Neural Regeneration Research 2025年第1期20卷 253-264页

作者： Zhengwei Hu Jing Yang Shuo Zhang Mengjie Li Chunyan Zuo Chengyuan Mao Zhongxian Zhang Mibo Tang Changhe Shi Yuming xu Department of Neurology The First Affiliated Hospital of Zhengzhou UniversityZhengzhou UniversityZhengzhouHenan ProvinceChina Academy of Medical Sciences of Zhengzhou University ZhengzhouHenan ProvinceChina NHC Key Laboratory of Prevention and treatment of Cerebrovascular Diseases The First Affiliated Hospital of Zhengzhou UniversityZhengzhou UniversityZhengzhouHenan ProvinceChina Henan Key Laboratory of Cerebrovascular Diseases The First Affiliated Hospital of Zhengzhou UniversityZhengzhou UniversityZhengzhouHenan ProvinceChina Institute of Neuroscience Zhengzhou UniversityZhengzhouHenan ProvinceChina Sino-British Research Centre for Molecular Oncology National Centre for International Research in Cell and Gene TherapySchool of Basic Medical SciencesAcademy of Medical SciencesZhengzhou UniversityZhengzhouHenan ProvinceChina Department of Gerontology The First Affiliated Hospital of Zhengzhou UniversityZhengzhou UniversityZhengzhouHenan ProvinceChina

The E3 ubiquitin ligase,carboxyl terminus of heat shock protein 70(Hsp70)interacting protein(CHIP),also functions as a co-chaperone and plays a crucial role in the protein quality control *** this study,we aimed to investigate the neuroprotective effect of overexpressed CHIP on Alzheimer’s *** used an adeno-associated virus vector that can cross the blood-brain barrier to mediate CHIP overexpression in APP/PS1 mouse *** overexpression significantly ameliorated the performance of APP/PS1 mice in the Morris water maze and nest building tests,reduced amyloid-βplaques,and decreased the expression of both amyloid-βand phosphorylated *** also alleviated the concentration of microglia and astrocytes around *** APP/PS1 mice of a younger age,CHIP overexpression promoted an increase in ADAM10 expression and inhibitedβ-site APP cleaving enzyme 1,insulin degrading enzyme,and neprilysin *** of HSP70 and HSP40,which have functional relevance to CHIP,were also *** nuclei transcriptome sequencing in the hippocampus of CHIP overexpressed mice showed that the lysosomal pathway and oligodendrocyte-related biological processes were up-regulated,which may also reflect a potential mechanism for the neuroprotective effect of *** research shows that CHIP effectively reduces the behavior and pathological manifestations of APP/PS1 ***,overexpression of CHIP could be a beneficial approach for the treatment of Alzheimer’s disease.

关键词： adeno-associated virus Alzheimer’s disease APP/PS1 mice carboxyl terminus of Hsp70 interacting protein gene therapy

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Vibration-induced acceleration of infiltration in loess

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Science China Earth Sciences 2021年第4期64卷 611-630页

作者： Jiading WANG Yuanjun xu Dengfei ZHANG Tianfeng gu State Key Laboratory of Continental Dynamics Department of GeologyNorthwest UniversityXi'an 710069China

Coupled effects of railway vibration, rainfall, and drying-wetting cycles contribute to new types of loess disasters such as the collapse and vibration-induced settlement of the railway subgrade, loess mass disintegration, and slope sliding,during the large-scale construction and operation of high-speed or heavy-haul railways in China. This has significant consequences for railways that require millimetre-level deformation control, seriously threatening railway operation and people's lives. Therefore, for the first time, "five vibration-induced effects" on loess subjected to vibration, water immersion, and drywetting cycles are discussed including the vibration-induced acceleration of cracking, infiltration, disintegration, sliding, and subsidence. In this paper, the vibration-induced acceleration of infiltration is discussed mainly. The response of loess to locomotive vibration along railways was determined using field sampling and data processing. The dominant frequency of locomotive vibration are 18-22 Hz and the maximum amplitude is 0.4 mm. Tests regarding the vibration-induced acceleration of the permeability were conducted on intact loess using a newly developed vibration permeability apparatus. The results reveal that the vibration accelerates the water permeability and increases the water saturation of loess. The permeability coefficient of saturated loess under vibration is ~1.5-20 times of that without vibration, reaching a maximum at a vibration frequency of 20 ***-induced infiltration tests were carried out on a loess column and the vibration-induced acceleration of water infiltration, wetting front evolution, and air outflow were analysed. The vibration-induced acceleration of infiltration strongly depends on the dry density, vibration frequency, and vibration amplitude. This pioneering work explores the mechnism of loess disasters triggered by locomotive vibration, rainfall, and drying-wetting cycles.

关键词： Loess disaster Locomotive vibration Five vibration-induced effects Vibration-induced acceleration of infiltration Air escape Loess landslide

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Suppressing nonlinear aeroelastic response of laminated composite panels in supersonic airflows using a nonlinear energy sink

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Chinese Journal of Aeronautics 2021年第2期34卷 376-385页

作者： Jian ZHOU Minglong xu Zhichun YANG Yingsong gu State Key Laboratory for Strength and Vibration of Mechanical Structures School of Aerospace EngineeringXi'an Jiaotong UniversityXi'an 710049China School of Aeronautics Northwestern Polyteclmical UniversityXi'an 710072China

This paper proposes using a Nonlinear Energy Sink(NES) to suppress the nonlinear aeroelastic response of laminated composite panels in supersonic airflows. Relevant aeroelastic equations are established using Hamilton’s principle and a finite element approach, drawing upon Von Karman’s large deflection theory and first order piston theory. The idea of the NES suppression region is proposed and the effects of NES parameters on the NES suppression region are studied in detail. The results show that the nonlinear aeroelastic responses of the panel can be completely suppressed by the Transient Resonance Capture(TRC);the appropriate NES parameter values can increase the critical dynamic pressure for flutter and suppress the nonlinear aeroelastic response effectively. Increasing the mass ratio of the NES can improve the NES suppression region;the nonlinear stiffness coefficient and damping of the NES within a specific range can suppress the nonlinear aeroelastic response. The most effective installation position for a NES is in a specific region behind the center-line of the panel in the direction of the airflow.

关键词： Aeroelasticity Laminated composite panel Nonlinear dynamics Nonlinear energy sink Panel flutter Passive suppression

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A Flexible Smart Healthcare Platform Conjugated with Artificial Epidermis Assembled by Three‑Dimensionally Conductive MOF Network for Gas and Pressure Sensing

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Nano-Micro Letters 2025年第2期17卷 601-620页

作者： Qingqing Zhou Qihang Ding Zixun Geng Chencheng Hu Long Yang Zitong Kan Biao Dong Miae Won Hongwei Song Lin xu Jong Seung Kim State Key Laboratory of Integrated Optoelectronics College of Electronic Science and EngineeringJilin UniversityChangchun 130012People’s Republic of China Department of Chemistry Korea UniversitySeoul 02841Republic of Korea TheranoChem Incorporation Seoul 02856Republic of Korea

The rising flexible and intelligent electronics greatly facilitate the noninvasive and timely tracking of physiological information in telemedicine *** building bionic-sensitive moieties is vital for designing efficient electronic skin with advanced cognitive functionalities to pluralistically capture external ***,realistic mimesis,both in the skin’s three-dimensional interlocked hierarchical structures and synchronous encoding multistimuli information capacities,remains a challenging yet vital need for simplifying the design of flexible logic ***,we construct an artificial epidermal device by in situ growing Cu_(3)(HHTP)_(2) particles onto the hollow spherical Ti_(3)C_(2)T_(x) surface,aiming to concurrently emulate the spinous and granular layers of the skin’s *** bionic Ti_(3)C_(2)T_(x)@Cu_(3)(HHTP)_(2) exhibits independent NO_(2) and pressure response,as well as novel functionalities such as acoustic signature perception and Morse code-encrypted message ***,a wearable alarming system with a mobile application terminal is self-developed by integrating the bimodular senor into flexible printed *** system can assess risk factors related with asthmatic,such as stimulation of external NO_(2) gas,abnormal expiratory behavior and exertion degrees of fingers,achieving a recognition accuracy of 97.6%as assisted by a machine learning *** work provides a feasible routine to develop intelligent multifunctional healthcare equipment for burgeoning transformative telemedicine diagnosis.

关键词： Ti_(3)C_(2)T_(x)@Cu_(3)(HHTP)_(2)composites NO_(2)/pressure flexible sensors Health-monitoring Machine learning

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