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Impact of the antiproliferative agent ciclopirox olamine treatment on stem cells proteome

World Journal of Stem Cells 2013年第1期5卷 9-25页

作者： gry h dihazi Asima Bibi Olaf Jahn Jessica Nolte Gerhard A Mueller Wolfgang Engel hassan dihazi Department of Nephrology and Rheumatology Georg-August University GoettingenD-37075 GoettingenGermany Proteomics Group Max-Planck-Institute of Experimental MedicineD-37075 GoettingenGermany Deutsche Forschungsgemeinschaft Research Center for Molecular Physiology of the Brain D-37073 Institute of human Genetics Georg-August University GoettingenD-37073 GoettingenGermany

AIM:To investigate the proteome changes of stem cells due to ciclopirox olamine (CPX) treatment compared to control and retinoic acid treated cells. METhODS:Stem cells (SCs) are cells, which have the ability to continuously divide and differentiate into various other kinds of cells. Murine embryonic stem cells (ESCs) and multipotent adult germline stem cells (maG-SCs) were treated with CPX, which has been shown to have an antiproliferative effect on stem cells, and compared to stem cells treated with retinoic acid (RA),which is known to have a differentiating effect on stem cells. Classical proteomic techniques like 2-D gel electrophoresis and differential in-gel electrophoresis (DIGE) were used to generate 2D protein maps from stem cells treated with RA or CPX as well as from non-treated stem cells. The resulting 2D gels were scanned and the digitalized images were collated with the help of Delta 2D software. The differentially expressed proteins were analyzed by a MALDI-TOF-TOF mass spectrometer, and the identified proteins were investigated and categorized using bioinformatics. RESULTS:Treatment of stem cells with CPX, a synthetic antifungal clinically used to treat superficial mycoses, resulted in an antiproliferative effect in vitro, without impairment of pluripotency. To understand the mechanisms induced by CPX treatments which results in arrest of cell cycle without any marked effect on pluripotency, a comparative proteomics study was conducted. The obtained data revealed that the CPX impact on cell proliferation was accompanied with a significant alteration in stem cell proteome. By peptide mass fingerprinting and tandem mass spectrometry combined with searches of protein sequence databases, a set of 316 proteins was identified, corresponding to a library of 125 non-redundant proteins. With proteomic analysis of ESCs and maGSCs treated with CPX and RA, we could identify more than 90 single proteins, which were differently expressed in both cell lines. We cou

关键词： Stem cells Differentiation hypusination Ciclopirox olamine Proteomics Retinoic acid

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免疫抑制正威胁着家禽的健康和福利

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国外畜牧学（猪与禽） 2005年第4期25卷 51-55页

作者： Mojtaba Yegani gry D.Butcher Amir h.Nilipour 罗天海加拿大Guelph大学美国佛罗里达州立大学巴拿马SA GrupoMeko 不详

家禽的遗传性抗病力有没有减弱?或者是不是有许多其它不明因子造成的条件使得家禽成为容易受到病原微生物攻击的目标?

关键词：免疫抑制家禽健康动物福利遗传抗病力免疫系统母源抗体主动免疫合并感染

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老龄小鼠模型内皮细胞衰老导致射血分数保留的心力衰竭

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中华高血压杂志 2017年第9期25卷 859-859页

作者：刘莉叶鹏 Gevaert AB Shakeri h Leloup AJ Van hove CE De Meyer gry Vrints CJ Lemmens K Van Craenenbroeck EM

由于全球老龄化,射血分数保留的心力衰竭（heart failure with preserved ejection fraction,hFpEF）发生率持续上升。虽然hFpEF的病理生理机制仍然不完全清楚,但通常认为内皮炎症发挥了核心作用。细胞衰老是一种与老化和炎症有关的细... 详细信息

由于全球老龄化,射血分数保留的心力衰竭（heart failure with preserved ejection fraction,hFpEF）发生率持续上升。虽然hFpEF的病理生理机制仍然不完全清楚,但通常认为内皮炎症发挥了核心作用。细胞衰老是一种与老化和炎症有关的细胞生长停滞过程。Gevaert等使用加速老化的小鼠（senescence-accelerated mice，SAM）模型探究细胞衰老在hFpEF发生发展中的作用。

关键词：细胞衰老射血分数心力衰竭老龄小鼠全球老龄化病理生理机制 ejection 内皮加速老化 preserved

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