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第十一届全国免疫学学术大会

作者： Wang Qihui Yang Huabing Liu Xiaoqing Dai Lianpan Ma Tong Qi Jianxun Wong Gary Peng Ruchao Liu Sheng Li Junfu Li Shihua Song Jian Liu Jianying He Jianhua Yuan Hui Xiong Ying Liao Yong Li Jianhua Yang Jianping Tong Zhou griffin bryan Bi Yuhai Liang Mifang Xu Xiaoning Qin Chuan Cheng Gong Wang Peiyi Qiu Xiangguo Kobinger Gary Shi Yi Yan Jinghua Gao Fu CAS Key Laboratory of Microbial Physiological and Metabolic Engineering Institute of Microbiology Chinese Academy of Sciences Shenzhen Key Laboratory of Pathogen and Immunity Shenzhen Third People’s Hospital School of Life Sciences Anhui University Jiangxi province center for disease control and prevention Research Network of Immunity and Health (RNIH) Beijing Institutes of Life Science Chinese Academy of Sciences CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of Microbiology Chinese Academy of Sciences CAS Center for Influenza Research and Early-warning (CASCIRE) Chinese Academy of Sciences School of Life Sciences University of Science and Technology of China Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases School of Medicine Tsinghua University Shanghai Institute of Applied Physics Chinese Academy of Sciences Ganzhou Prefecture Center for Disease Control and Prevention Special Pathogens Program National Microbiology Laboratory Public Health Agency of Canada National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention (China CDC) MRC Human Immunology Unit University of Oxford Key Laboratory of Human Diseases Comparative Medicine Ministry of Health Institute of Medical Laboratory Animal Science Chinese Academy of Medical Sciences Faculty of Biological Science University of Leeds College of Life Sciences University of Chinese Academy of Sciences Savaid Medical School University of Chinese Academy of Sciences

Zika virus infections are associated with fetal microcephaly,neurological complications and constitute a public health ***,we report the isolation of 13 specific,human-origin monoclonal antibodies from an imported Zika case to *** of the isolated antibodies(Z23 and Z3L1)demonstrate potent Zika-specific neutralization in vitro without binding or neutralizing activity against Zika’s closest relative Dengue virus(DENV)1-4,

关键词： Zika virus envelope protein human neutralizing MAbs B cell sequencing crystal structure Cryo-EM

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Hydrothermal carbonization of livestock mortality for the reduction of pathogens and microbially-derived DNA

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Frontiers of Environmental Science & Engineering 2017年第3期11卷 107-114页

作者： Thomas F. Ducey Jessica C. Collins Kyoung S. Ro bryan L. Woodbury D. Dee griffin Coastal Plains Soil Water and Plant Research Center Agricultural Research Service United States Department of Agriculture Florence SC 29501 USA South Carolina Governor's School for Science and Mathematics Hartsville SC 29550 USA U.S. Meat Animal Research Center Agricultural Research Service United States Department of Agriculture Clay Center NE 68933 USA University of Nebraska-Lincoln Great Plains Veterinary Education Center Lincoln NE 68588 USA

Hydrothermal carbonization （HTC）, utilizing high temperature and pressure, has the potential to treat agricultural waste via inactivating pathogens, antibiotic resistance genes （ARG）, and contaminants of emerging concern （CEC） in a environmental and economical manner. Livestock mortality is one facet of agricultural waste that can pose a threat to the surrounding environment. While several methods are utilized to treat livestock mortality, there remains a paucity of data on the elimination of microbially- derived DNA in these treatment practices. This DNA, most notably ARGs, if it survives treatment can be reintroduced in agricultural environments where it could potentially be passed to pathogens, posing a risk to animal and human populations. HTC treatments have been successfully utilized for the treatment of CECs, however very little is understood on how ARGs survive HTC treatment. This study aims to fill this knowledge gap by examining the survivability ofmicrobially-derived DNA in the HTC treatment of livestock mortality. We examined three treatment temperatures （100℃, 150℃, and 200℃） at autogenic pressures at three treatment times （30, 60, and 240 min）. We examined the amplification of a plasmid-bome reporter gene carried by Escherichia coli DH 10B introduced to both beef bone and tissue. Results indicate that while all three temperatures, at all treatment times, were suitable for complete pathogen kill, only temperatures of 150℃ and 200℃ were sufficient for eliminating microbial DNA. These results serve as the basis for future potential HTC treatment recommendations for livestock mortality when considering the elimination of pathogens and ARGs.

关键词： High-temperature carbonization Microbial DNA Livestock mortality

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Seashore paspalum (Paspalum vaginatum Sw.) performance under shade in multi-environment trials

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Grass Research 2021年第1期1卷 42-49页

作者： Beatriz Tome Gouveia Paul Raymer Brian M.Schwartz Esteban F.Rios Kevin E.Kenworthy J.bryan Unruh Charles Fontanier Esdras M.Carbajal Jason Kruse Natasha K.Restuccia Susana R.Milla-Lewis Department of Crop and Soil Sciences North Carolina State UniversityCampus Box 7620RaleighNC 27695USA Department of Crop and Soil Sciences University of GeorgiaGriffin Campus1109 Experiment StreetGriffinGA 30223USA Department of Crop and Soil Sciences University of GeorgiaTifton Campus2360 Rainwater RoadTiftonGA 31793USA Agronomy Department University of Florida2005 SW 23rd Street Bldg 350GainesvilleFL 32608USA Environmental Horticulture Department University of FloridaWest Florida Research and Education Center4253 Experiment RoadHwy 182JayFL 32565USA Department of Horticulture and Landscape Architecture Oklahoma State UniversityOklahomaOK 74078USA Department of Environmental Horticulture University of Florida1541 Fifield HallGainesvilleFL 32611USA

Shade caused by trees or buildings can affect the quality and growth of ***,breeding for shade tolerance is an essential component of most turfgrass improvement *** objective of this study was to evaluate the performance of seashore paspalum(Paspalum vaginatum Sw.)breeding lines under shade in multi-environment *** sources were 20 lines from the University of Georgia seashore paspalum breeding program and two checks,the cultivars'SeaDwarf'and'SeaStar'.Field trials were conducted from 2016 to 2019 under shade structures designed to reduce ambient sunlight at three locations:Citra(FL),Tifton(GA),and Raleigh(NC).The response variables evaluated were percent living ground cover(%GC),dark green color index(DGCI),canopy height(CH)and turfgrass quality(TQ).Data were analyzed by mixed model approaches using ASReml-R,and the t-statistics were used to group the *** genetic variances were observed in the single-location-repeated-measures analysis for CH and DGCI at Citra and Tifton,%GC at Citra,and TQ at *** correlations of the predicted values for entries between locations ranged from low to moderate,with the exception of between Citra and Tifton(0.76)for *** were a few breeding lines with superior performance to the entry average for all traits for which the entry variance was *** conclusion,genetic variability was observed for all traits and superior seashore paspalum breeding lines for multiple traits were identified under shade.

关键词： breeding cultivar measures

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