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Targeted quantitative profiling of metabolites and gene transcripts associated with 4-aminobutyrate (GABA) in apple fruit stored under multiple abiotic stresses

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Horticulture research 2018年第1期5卷 66-79页

作者： Carolyne J.Brikis Adel Zarei greta Z.Chiu Kristen L.Deyman Jingyun Liu Christopher P.Trobacher Gordon J.Hoover Sanjeena Subedi Jennifer r.DeEll Gale G.Bozzo Barry J.Shelp Department of Plant Agriculture University of GuelphGuelphOntarioN1G 2W1Canada Department of Mathematical Sciences Binghamton UniversityBinghamtonNY13902USA Ontario Ministry of Agriculture Food and Rural AffairsBox 5871283 Blueline Rd.at Highway 3SimcoeOntarioN3Y 4N5Canada

4-Aminobutyrate accumulates in plants under abiotic ***,targeted quantitative profiling of metabolites and transcripts was conducted to monitor glutamate-and polyamine-derived 4-aminobutyrate production and its subsequent catabolism to succinate or 4-hydroxybutyrate in apple(Malus x domestica Borkh.)fruit stored at 0°C with 2.5 kPa O2 and 0.03 or 5 kPa CO2 for 16 ***-temperature-induced protein hydrolysis appeared to be responsible for the enhanced availability of amino acids during early storage,and the resulting higher glutamate level stimulated 4-aminobutyrate levels more than *** CO2 increased the levels of polyamines,as well as succinate and 4-hydroxybutyrate,during early storage,and 4-aminobutyrate and 4-hydroxybutyrate over the longer *** of all of the genes likely involved in 4-aminobutyrate metabolism from glutamate/polyamines to succinate/4-hydroxybutyrate was induced in a co-ordinated ***2-regulated expression of apple GLUTAMATE DECArBOXYLASE 2,AMINE OXIDASE 1,ALDEHYDE DEHYDrOGENASE 10A8 and POLYAMINE OXIDASE 2 was evident with longer term *** suggested that respiratory activities were restricted by the elevated CO2/O2 environment,and that decreasing NAD+availability and increasing NADPH and NADPH/NADP+,respectively,played key roles in the regulation of succinate and 4-hydroxybutyate ***,these findings suggest that both transcriptional and biochemical mechanisms are associated with 4-aminobutyrate and 4-hydroxybutyrate metabolism in apple fruit stored under multiple abiotic stresses.

关键词： hydrolysis subsequent restricted

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委内瑞拉圭亚那地盾含金石英脉的应变:一种有效的评价矿床方法

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世界地质 1993年第1期 112-113页

作者： Dennis P.cos greta L.Orris 周文林

美国地质调查局和Tecnica Minera C.A合作对圭亚那地盾区的矿床进行综合研究长达5年之久,最终成果包括一份地质图,一些地球物理资料,500个矿床记录资料以及矿床评价等。该地盾包括太古宙麻粒岩,晚元古的花岗岩绿岩带,具有过渡性质的沉积... 详细信息

美国地质调查局和Tecnica Minera C.A合作对圭亚那地盾区的矿床进行综合研究长达5年之久,最终成果包括一份地质图,一些地球物理资料,500个矿床记录资料以及矿床评价等。该地盾包括太古宙麻粒岩,晚元古的花岗岩绿岩带,具有过渡性质的沉积-火山组合是由大陆碎屑岩和大量的未变质的火山凝灰岩以及伴生的花岗岩组成。比较年轻的沉积组合由未变形的中、晚元古的大陆碎屑沉积岩组成,它的局部有晚期花岗岩和碱性岩侵入。

关键词：含金石英脉矿床模型矿床矿藏地盾地台圭亚那南美洲

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数字时代的社会保障——应对社会保障体系的新挑战和机遇

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国际社会科学杂志（中文版） 2022年第3期39卷 101-125,6,7,10页

作者：多米尼克·拉沙勒格列塔·卡托切蒂王爱松(译) 国际社会保障协会社会发展部国际劳工组织不详

数字技术如今渗透到人类活动的各个方面。这些技术正在产生全新的经济部门。例如,数字平台以很低的成本瞬间让产品和服务的供给与需求相匹配,从而创造新的需求和新的机会。然而与此同时,数字平台也打破了传统的雇主一雇员关系,将工作碎... 详细信息

数字技术如今渗透到人类活动的各个方面。这些技术正在产生全新的经济部门。例如,数字平台以很低的成本瞬间让产品和服务的供给与需求相匹配,从而创造新的需求和新的机会。然而与此同时,数字平台也打破了传统的雇主一雇员关系,将工作碎片化为一系列的任务,从而改变工作的性质。本文考察了社会保障机构如何才能利用这些机会并应对新兴数字经济带来的挑战。为此,本文首先列举我们预期社会保障可以发挥的作用,然后考察数字经济是如何支持或者阻碍社会保障发挥这些作用的。

关键词：数字平台数字时代挑战和机遇社会保障体系供给与需求碎片化发挥的作用雇员关系

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Effect of Gastrointestinal Protease Digestion on Bioactivity of Marine Peptides

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Journal of Agricultural Science and Technology(B) 2014年第1期4卷 74-84页

作者： Ida-Johanne Jensen Lisa Lystbek Andersen Carlo Gunnar Ossum greta Jakobsen Cristian De Gobba Sabeena Farvin Koduvayur Habeebullah Inez Johansson Else Hoffmann Edel Oddny Elvevoll Flemming Jessen Henrik Hauch Nielsen Faculty of Bioscience Fisheries and Economics Norwegian College of FisheO Science UniversiO of Tromso Tromso 9037Norway Division ofIndustrial Food research National Food Institute DTU-Food Technical University qf Denmark Lyngby DK-2800Denmark Department of Biology Cell and Developmental Biology Universitv of Copenhagen Copenhagen 2100 Denmark Marinova ApS Adelvej 11 Lem St. 6940 Denmark Department of Food Science University o[Copenhagen Frederiksberg C 1958 Denmark

Focus in nutritional science has turned towards components in, or added to, foods that may possess health beneficial activities beyond the classical nutritional value, namely functional food. Bioactive peptides are examples of such components. In vitro studies on bioactivities have mainly been executed without concerning subsequent digestion after intake and the aim of this work was hence to investigate how the in vitro antioxidative, antihypertensive and caspase activating activities of peptides are affected by digestion with gastrointestinal (GI) proteases. Five different fish protein hydrolysates were chosen to study the effect of in vitro digestion on bioactivity. The protein concentration decreased in all samples during digestion and the molecular weight distribution of the peptides shifted towards lower values. Thus, in vitro digestion with GI proteases resulted in a further degradation of the peptides obtained by hydrolysis. The antihypertensive effect increased in all samples after digestion with GI proteases whereas the antioxidative capacity decreased. The effect on the caspase activity depended on the proteases used in the preparation of hydrolysates. In conclusion, the caspase activity and antihypertensive activity are maintained during digestion with GI proteases, while the antioxidative capacity seems to be reduced.

关键词：生物活性肽体外消化肠蛋白酶海洋肽胃肠道 caspase 蛋白水解物抗氧化能力

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Kinetic Dependences of Two-Stage Dilute Acid and Enzyme Hydrolysis of Paulownia tomentosa

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Journal of Chemistry and Chemical Engineering 2011年第11期5卷 985-989页

作者： greta radeva Ivo Valchev Stoiko Petrin University of Chemical Technology and Metallurgy Sofia Bulgaria

The sugars potential of Paulownia tomentosa is estimated by dilute acid pretreatment and cellulase hydrolysis. The kinetics of dilute (1%) sulfuric acid hydrolysis is studied at temperatures of 100 oC, 120 oC and 130 oC, while the kinetics of the subsequent enzyme hydrolysis is examined at a temperature of 50 oС and reaction time varied from 60 to 300 min using cellulase complex NS 50013 and β-glucosidase NS 50010. The reducing sugars formation is modeled as a pseudo-homogeneous first order reaction in view of the kinetics of dilute sulfuric acid hydrolysis. The results obtained indicate that the reaction proceeds in an energetically homogeneous system (E = const) providing identical accessibility of the reagent to the hydrolyzing sites (A = const). The enzyme hydrolysis kinetics follows heterogeneous catalytic mechanism. The process is described by an exponential kinetic equation, which is well recognised in case of processes on uniformly inhomogeneous surfaces. The current rate decreases significantly probably because of exhaustion of the available active sites on the surface and steric hindrances due to the presence of lignin. This investigation provides information of importance for the fermentation step of the bio-ethanol production process.

关键词：纤维素酶水解水解动力学毛泡桐依赖性葡萄糖苷酶稀酸反应时间动力学方程

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Global Financial Crisis and Accounting rules： The Implications of the New Exposure Draft （ED） Financial Instruments： Expected Credit Losses on the Evaluation of Banking Company Loans

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Journal of Modern Accounting and Auditing 2013年第9期9卷 1141-1162页

作者： Gianluca risaliti greta Cestari Mariarita Pierotti University of Napoli Parthenope Napoli Italy University of Ferrara Ferrara Italy University of Milano-Bicocca Milan Italy

During the financial crisis, the delayed recognition of credit losses on loans and other financial instruments was identified as a weakness in existing incurred loss model of impairment stated by International Accounting Standards (IAS) 39, because it is believed that this delay might generate pro-cyclical effects. In response to the recommendations of G20, Financial Crisis Advisory Group (FCAG), and other international bodies, the International Accounting Standards Board (IASB) has undertaken, since 2009, as a part of the project to replace IAS 39, a project (partially shared with Financial Accounting Standards Board (FASB)) aimed at introducing an expected loss model of impairment. Within the scope of this subset project, the IASB has previously issued two exposure documents proposing models to account for expected credit losses: an exposure draft (ED) Financial Instrument: Amortized Cost and Impairment, published in November 2009, and a supplementary document (SD) Financial Instrument: Impairment, published jointly with the FASB in January 2011. However, neither of the two proposals received strong support from interested parties. recently, the IASB, after the FASB"s decision to withdraw from the joint project and to develop a separate expected credit loss model based on a single measurement approach consisting in the sole recognition of lifetime expected credit losses, published a third proposal?the so-called "expected credit losses" model (ED/2013/3 Financial Instruments: Expected Credit Losses).

关键词：损失模型会计准则金融工具金融危机信用贷款国际机构银行

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Mood disorders: A potential link between ghrelin and leptin on human body?

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World Journal of Experimental Medicine 2015年第2期5卷 103-109页

作者： Stalo Zarouna greta Wozniak Anastasia Ioannis Papachristou Psychology Department University of Cyprus Basic Medical Science Medical Pharmacology Medical School University of Cyprus School of Medicine University of Crete Voutes Campus

Leptin and ghrelin are two hormones associated with multiple physiological functions, especially energy balance. Leptin is an adipocyte-secreted hormone discovered in 1950 and ghrelin which was found in 1999, is a peptide hormone produced and secreted in the stomach. A number of previous studies showed that these hormones could be associated with different types of mood disorders. The results of previous studies, nevertheless, are confounded by diverse sample selection and different methodologies. A search for related articles in the Pub Med database was attempted. The search covered studies, reports, reviews and editorials published in the last ten years. Older references served as auxiliary sources for comparison purposes. However, due to the different results of the studies, there is a need for more investigation in order to establish the exact biochemical mechanisms that are responsible for these diseases and ghrelin's and leptin's effects on mood.

关键词： Mood disorders Ghrelin Leptin

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Percutaneous approach to treatment of coronary disease in a patient with uremic cardiomyopathy

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World Journal of Cardiology 2011年第4期3卷 117-120页

作者： Gianluca Petrillo Plinio Cirillo Maria Prastaro greta Luana D'Ascoli Federico Piscione Department of Internal Medicine Cardiovascular and Immunological Sciences University of Naples "Federico II" via Sergio Pansini

Uremic cardiomyopathy is chronic ischemic left ventricular dysfunction characterized by heart failure, myocardial ischemia, hypotension in dialysis and arrhythmia. This nosologic entity represents a leading cause of morbidity and mortality among patients with endstage renal disease receiving long-term hemodialysis. It is intuitive that revascularization in the presence of coronary artery disease in these patients represents an effective option for improving their prognosis. Although the surgical option seems to be followed by the best clinical outcome, some patients refuse this option and others are not good candidates for surgery. The present report describes the case of a patient affected by uremic cardiomyopathy and severe coronary artery disease in whom revascularization with percutaneous coronary angioplasty was followed by a significant improvement in quality of life.

关键词： Coronary artery disease Heart failure Percutaneous coronary intervention Uremic cardiomyopathy

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女人,水,能源:生态女性主义路径

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鄱阳湖学刊 2015年第1期 116-128页

作者：格里塔.加德文李莉韦清琦威斯康辛大学河瀑分校南京师范大学外国语学院南京师范大学金陵女子学院

生态女性主义视角如何帮助我们理解并回应当今所要面对的水污染及能源生产问题?本文以当前的几个案例——"曼尼托巴水利"的阿诺海德—韦斯顿工程、"萨默斯能源2号"以及哥伦比亚河大坝——来揭示公司对本属于人民及土地的水能的擅用。生... 详细信息

生态女性主义视角如何帮助我们理解并回应当今所要面对的水污染及能源生产问题?本文以当前的几个案例——"曼尼托巴水利"的阿诺海德—韦斯顿工程、"萨默斯能源2号"以及哥伦比亚河大坝——来揭示公司对本属于人民及土地的水能的擅用。生态女性主义阐明了关于水、权力、人际关系的性别化与文化假定是如何引导了水利基础设施的建构的,而这样的基础建设又延续了环境性别歧视、环境种族歧视和环境阶级歧视。作为替代的选择,走向水资源公正的生态女性主义路径倡导的策略,能够生发生态民主、生态经济学以及伙伴关系的文化,在其中水与能源自由通畅地流淌。

关键词：水生态女性主义权力

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呼吸平均CT衰减校正在心肌灌注SPECT／CT显像中的作用

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中华核医学与分子影像杂志 2017年第10期37卷 673-673页

作者：顾佳唐军苏州大学医学部放射医学与防护学院 215123 苏州大学附属第二医院核医学科 215004

目的电影平均CT（CACT）和内插平均CT（IACT）已被报道可用于改善PET／CT肿瘤和心脏显像的衰减校正（AC）。该研究旨在通过计算机模拟和物理模型实验评估CACT和IACT在心肌灌注SPECT／CT显像中的有效性。方法先采用呼吸运动幅度为2、3、... 详细信息

目的电影平均CT（CACT）和内插平均CT（IACT）已被报道可用于改善PET／CT肿瘤和心脏显像的衰减校正（AC）。该研究旨在通过计算机模拟和物理模型实验评估CACT和IACT在心肌灌注SPECT／CT显像中的有效性。方法先采用呼吸运动幅度为2、3、4cm的数字XCAT模型模拟正常男性99Tcm-MIBI的分布。平均活度和衰减图分别表示静态的SPECT和CACT，而吸气末和呼气末的衰减图分别代表2个螺旋CT（HCTs）。采用平行孔分析投影仪对60个无噪声和有噪声的投影进行超过180。的模拟。将673MBq的99Tcm填人拟人躯干模型（模型有正常的心脏或有可在呼吸平台上编程的心肌灌注缺损区，以此模拟各种呼吸幅度）中，使用SPECT／CT经180。采集60个投影，同时采集CACT、标准HCT和在极端期的2个HCTs。基于以上数据，内插CT时相在使用仿射加B样条配准融合时产生，IACT通过模拟和模型实验中平均化内插CT时相和2个原始极端期而获得。采集的投影经使用CACT、IACT和HCTs衰减校正后进行重建。极坐标靶心图和17段图经过吸收相对差（rD）的分析。2个感兴趣区（rOI）分别勾画在灌注缺损区和本底区以获得强度比（Ir）。结果在模拟研究中．不同衰减校正方法产生的极坐标靶心图无显著差异，而定量rD检测表明，SPECT㈨T与原始模型最相似，其次是rDmax〈8％和〈10％的spEcyl栅。SPECTHCTs的rD值偏离原始模型和各个心肌节段的SPECTCACT，rDmax在模拟实验和模型实验中分别为19．76％和16．68％。SPECTHCTs的Ir值因实际更高的运动振幅而波动性高。结论，与HCT-AC相比，CACT-AC和IACT-AC均可减轻呼吸伪影并改善心肌灌注SPECT显像的定量。IACT的使用进一步降低了辐射剂量。

关键词： SPECT/CT 心肌灌注衰减校正呼吸伪影

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