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Laser capture microdissection and genetic analysis of carbon-labeled Kupffer cells

World Journal of Gastroenterology 2009年第14期15卷 1708-1718页

作者： Stephan Gehring Edmond Sabo maryann E San martin Elizabeth m Dickson Chao-Wen Cheng Stephen H gregory Department of medicine Rhode Island Hospital and The Warren Alpert Medical School of Brown University Providence RI 02903 United States Department of Pathology Rhode Island Hospital and The Warren Alpert Medical School of Brown University Providence RI 02903 United States zentrum fur Kinder- und Jugendmedizin der Universitaitsmedizin der Johannes Gutenberg-Universitait 55101 Mainz Germany

AIm： To develop a method of labeling and microdissecting mouse Kupffer cells within an extraordinarily short period of time using laser capture microdissection （LCm）. mETHODS： Tissues are complex structures comprised of a heterogeneous population of interconnected cells. LCm offers a method of isolating a single cell type from specific regions of a tissue section. LCm is an essential approach used in conjunction with molecular analysis to study the functional interaction of cells in their native tissue environment. The process of labeling and acquiring cells by LCm prior to mRNA isolation can be elaborate, thereby subjecting the RNA to considerable degradation. Kupffer cell labeling is achieved by injecting India ink intravenously, thus circumventing the need for in vitro staining. The significance of this novel approach was validated using a cholestatic liver injury model. RESULTS： mRNA extracted from the microdissected cell population displayed marked increases in colonystimulating factor-1 receptor and Kupffer cell receptor message expression, which demonstrated Kupffer cell enrichment. Gene expression by Kupffer ceils derived from bile-duct-ligated, versus sham-operated, mice was compared. microarray analysis revealed a significant （2.5-fold, q value 〈 10） change in 493 genes. Based on this fold-change and a standardized Pubmed search, 10 genes were identified that were relevant to the ability of Kupffer cells to suppress liver injury. CONCLUSION; The methodology outlined herein provides an approach to isolating high quality RNA from Kupffer cells, without altering the tissue integrity.

关键词： Kupffer cells India ink Laser capturemicrodissection Bile duct ligation DNA microarray

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机器人工作室的通信结构

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国内外机电一体化技术 2000年第3期3卷 6-7页

作者： m.mueller D.Rembold Prof.Dr.-lng.H.Woern 刘云男

在Karlsruhe大学的实时控制和机器人(IPR)研究所,正在研制一套智能机器人系统,这台机器人将用于(产品、图书等)目录销售公司的运输部门从事搬运货物。该项目的名称是'智能传感器、执行器系统'(ISAS),它是由bmb+f(德国联邦教育研究科学... 详细信息

在Karlsruhe大学的实时控制和机器人(IPR)研究所,正在研制一套智能机器人系统,这台机器人将用于(产品、图书等)目录销售公司的运输部门从事搬运货物。该项目的名称是'智能传感器、执行器系统'(ISAS),它是由bmb+f(德国联邦教育研究科学与技术部)设立的项目。

关键词：机器人工作室通信结构微控制器智能机器人系统执行器传感器

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Liver stiffness in pregnant women with intrahepatic cholestasis of pregnancy:A case control study

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World Journal of Hepatology 2023年第7期15卷 904-913页

作者： Juliane Nees Franziska J Ammon Johannes mueller Herbert Fluhr Sebastian mueller Department of Gynecology and Obstetrics University Hospital HeidelbergHeidelberg 69120Germany Center for Alcohol Research University Hospital HeidelbergHeidelberg 69120Germany Division of Obstetrics Department of Obstetrics and GynecologyMedical University of GrazGraz 8036Austria

BACKGROUND Intrahepatic cholestasis of pregnancy(ICP)is a rare but severe complication for both the mother and the unborn *** diagnosis is primarily based on elevated serum levels of bile *** a large ICP cohort,we here study in detail liver stiffness(LS)using transient elastography(TE),now widely used to noninvasively screen for liver cirrhosis within *** To specifically explore LS in a large cohort of women with ICP compared to a control group with uncomplicated *** LS and hepatic steatosis marker controlled attenuation parameter(CAP)were measured in 100 pregnant women with ICP using TE(Fibroscan,Echosens,Paris,France)between 2010 and *** 17 cases,LS could be measured postpartum.450 women before and 38 women after delivery with uncomplicated pregnancy served as control *** laboratory,levels of bile acids and apoptosis marker caspase-cleaved cytokeratin 18 fragment(m30)were also *** Women with ICP had significantly elevated transaminases but normal gammaglutamyl transferase(GGT).mean LS was significantly increased at 7.3±3.0 kPa compared to the control group at 6.2±2.3 kPa(Pm LS decreased significantly in both groups but was still higher in ICP(5.8±1.7 kPa vs 4.2±0.9 kPa,Pm30 but not *** correlation was seen with GGT that even increased significantly after delivery in the ICP *** acids were mostly correlated with the liver apoptosis marker m30,LS and levels of alanine aminotransferase,aspartate aminotransferase,and *** multivariate analysis,LS remained the sole parameter that was independently associated with elevated bile *** In conclusion,LS is significantly elevated in ICP which is most likely due to toxic bile acid accumulation and hepatocyte *** association with conventional laboratory markers,LS provides additional non-invasive informati

关键词： Intrahepatic cholestasis of pregnancy Transient elastography Bile acids Liver stiffness High risk pregnancy

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Friction characteristics of confined inflatable structures

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Friction 2014年第4期2卷 365-390页

作者： Eduardo m.SOSA gregory J.THOmPSON Ever J.BARBERO Subhadeep GHOSH Kevin L.PEIL Department of Civil and Environmental Engineering West Virginia UniversityMorgantownWV 26506-6103USA Department of mechanical and Aerospace Engineering West Virginia UniversityMorgantownWV 26506-6106USA

The availability of high‐strength fabrics and progress in the development of large‐scale inflatable technology made possible the creation of temporary and quickly deployable structures for protection of underground *** structures are relatively lightweight and portable,and can maintain the required rigidity while in *** benefits have prompted the development of inflatable structures for use in confined spaces,such as tunnels and large‐diameter pipes to act as barriers for containing flooding with minimal infrastructure *** work presents experimental results obtained from the evaluation of frictional characteristics of the fabric material that constitute the structural membrane of confined inflatable structures developed for protection of underground transportation tunnels and other large *** tests at coupon level and slippage tests in a reduced‐scale inflatable structure were performed in order to evaluate the frictional characteristics of Vectran *** at coupon level were performed to determine the friction coefficient for different surface types and *** with the reduced‐scale inflatable structure contributed to the understanding of the friction characteristics at system level when subjected to different pressurization or depressurization sequences designed to induce *** results indicate that friction coefficient values at coupon level are about 29 percent higher than values derived from reduced‐scale tests.

关键词： friction fabric materials inflatable structures slippage Vectran

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Simple glycolipids of microbes:Chemistry,biological activity and metabolic engineering

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Synthetic and Systems Biotechnology 2018年第1期3卷 3-19页

作者： Ahmad mohammad Abdel-mawgoud gregory Stephanopoulos Department of Chemical Engineering Massachusetts Institute of Technology77 Massachusetts Ave.CambridgeMA 02139USA

Glycosylated lipids(GLs)are added-value lipid derivatives of great *** their interesting surface activities that qualify many of them to act as excellent ecological detergents,they have diverse biological activities with promising biomedical and cosmeceutical ***,especially those of microbial origin,have interesting antimicrobial,anticancer,antiparasitic as well as immunomodulatory ***,GLs are hardly accessing the market because of their high cost of *** believe that experience of metabolic engineering(mE)of microbial lipids for biofuel production can now be harnessed towards a successful synthesis of microbial GLs for biomedical and other *** review presents chemical groups of bacterial and fungal GLs,their biological activities,their general biosynthetic pathways and an insight on mE strategies for their production.

关键词： Biosurfactant Glycolipids biosynthesis Glycosyl/acyl transferases Glycosides Physiological roles Lipid biotechnology

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Alzheimer’s Disease: A Washing machine on the Fritz

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Journal of Behavioral and Brain Science 2022年第4期12卷 131-163页

作者： James m. gregory Professor Emeritus Texas Tech University Lubbock TX USA

A mathematical model has been developed to numerically model the risk of developing Alzheimer’s disease and Chronic Traumatic Encephalopathy (CTE) as a person ages. The model was programmed in Excel to provide a working prototype computer simulation model. The model provides estimates of the cumulative risk of developing Alzheimer’s disease and CTE as age increases. A one-year step size was used. The model has two major parts: one predicts changes in slow-wave sleep as a person ages and the second component adjusts the flushing efficiency of waste products from the brain. The two components work together and interact to lower the flushing of waste components as age increases. The development of the model provides an overview of how the various factors work together that lead to the onset of Alzheimer’s disease and the associated CTE. Calibration of the coefficients in the model is based on published data sets presented in the literature. Further research and refinement of calibration coefficients should be explored.

关键词： Alzheimer’s Disease Sleep mathematical modeling Chronic Traumatic Encephalopathy CTE Aging Depression Amyloid β Tau

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Alzheimer’s Disease: An Analysis of Gender Effects

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Journal of Behavioral and Brain Science 2022年第10期12卷 455-473页

作者： James m. gregory Texas Tech University Lubbock TX USA

Alzheimer’s disease is a dreaded outcome that affects both men and women in later years of life. While root causes of this form of dementia are not clear, various factors are known that contribute to the risk of development and the reduction in risks based on gender and our choices in life. This paper evaluates the various factors that affect the risks of developing Alzheimer’s disease as we age. The focus of this paper is gender considerations in a mathematical model programmed in Excel. The model was first presented by gregory [1] and was calibrated on one of the original population data sets with 50 - 50 male and female participants. This model overpredicted the risks for women and underpredicted the risks for men. A solution to this problem was found based on published values of sex hormones for men and women. Based on the expanded current model, two major factors contribute to the gender differences in predictions: gender factor values (10.5 ml·kg-1·min-1 for men and 3.5 ml·kg-1·min-1 for women) used in VO2max equations and a sex hormone factor that changes as hormones change for men and women with age. Smoking differences associated with gender and the risk associated with smoking was added to the model. Cognitive reserve based on education differences between men and women was also added. These are minor components compared to hormone effects. The expanded model includes an input for unsaturated fat diets and cholesterol reducing medications and use of Viagra by men that is known to reduce risk of developing Alzheimer’s disease. Predictions from the expanded model closely matched measured values from age 50 through age 95 for each gender with R2 values of 0.99, which were highly significant (p = 0.001). The expanded model predictions matched the reduced lifetime risk for men and women associated with a data set that included a population with the opportunity to use statins and Viagra. The expan

关键词： Gender Effects Alzheimer’s Disease Dementia mathematical modeling Statins Viagra Cholesterol Unsaturated Fat Diets

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WASO: Why Does It Increase with Age?

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Journal of Behavioral and Brain Science 2022年第4期12卷 164-176页

作者： James m. gregory Professor Emeritus Texas Tech University Lubbock TX USA

Insomnia is a common sleep disorder, especially for seniors. WASO (time awake after sleep onset), a component of insomnia, tends to increase with age. There are also many variations in WASO for seniors. To better understand the nature of insomnia, an equation was developed to predict WASO using maximum oxygen uptake (VO2max) as a measure of fitness associated with aerobic exercise. Predictions from the equation matched measured values with an R2 = 0.98. The results were highly significant (p 2max as a function of age and relative fitness (a measure of aerobic fitness independent of age). The two equations were combined to predict WASO as a function of age, gender, and relative fitness. Based on these results, it appears that aerobic exercise to improve relative fitness can be used to reduce WASO. The WASO model explains the numerous reports of reduced WASO associated with long-term exercise programs. The model also explains why WASO increases with age and why high WASO values associate with early death.

关键词： WASO Time Awake Sleep Disorders Sleep Quality Aerobic Exercise

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mathematical model for Stroke and White matter Hyperintensities

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Journal of Behavioral and Brain Science 2023年第4期13卷 66-94页

作者： James m. gregory Professor Emeritus Civil Engineering Department at Texas Tech University Lubbock TX USA

A mathematical model was developed to predict the risk of having a stroke as a person ages. The age component was derived from the concept that the change in risk of stroke with age is a function of the current risk of developing a stroke. This equation modeled the trend with age reported in the literature for two different data sets with R2 values of 0.97 or better for both men and women. A second equation of a similar nature was developed to predict the accumulation of white matter hyperintensities, WmH, as a person ages. It appears that each equation includes a set of common risk factors. This set of common risk factors allows an individual’s risk for stroke to be based on measured WmH. A third equation links WmH with the risk of developing a stroke. This equation allows an individual to use measured WmH from brain scans to predict the future risk of developing a stroke. In theory, a person with a relatively high measurement of WmH can project future risk for stroke with age and use counter measures such as exercise and medications to keep other risk factors low as a person continues to age.

关键词： Stroke White matter Hyperintensities WmH Aging Depression Trauma PTSD

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Ordered SrTiO_3 Nanoripples Induced by Focused Ion Beam

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Nano-micro Letters 2012年第4期4卷 243-246页

作者： Jiang Wu Gang Chen Zhaoquan Zeng Shibin Li Xingliang Xu Zhiming m.Wang gregory J.Salamo State Key Laboratory of Electronic Thin Films and Integrated Devices University of Electronic Science and Technology of China Institute of Intelligent Optoelectronic Technology Zhejiang University of Technology Institute for Nanoscience and Engineering University of Arkansas

Ordered nanoripples on the niobium-doped SrTiO_3 surfaces were fabricated through focused ion beam bombardment. The surface morphology of the SrTiO_3 nanoripples was characterized using in situ focused ion beam/scanning electron microscopy. The well-aligned SrTiO_3 nanostructures were obtained under optimized ion irradiation conditions. The characteristic wavelength was measured as about 210 nm for different ion beam currents. The relationship between the ion irradiation time and current and SrTiO_3 surface morphology was analyzed. The presented method will be an effective supplement for fabrication of SrTiO_3 nanostructures that can be used for ferroelectric and electronic applications.

关键词： SrTiO3 focused ion beam nanoripple self-assembly

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