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Compassion in Buddhism and Guanxi： Can There Be a Synergy for Westem Companies in China

Chinese Business Review 2013年第4期12卷 287-297页

作者： Tashi Gelek gration david BSL (Business School Lausanne) Lausanne Switzerland Phonak Communications AG Murten Switzerland BSL (Business School Lausanne) Lausanne Switzerland

Compassion is one of the core fundamental elements of Buddhism. The compassionate virtues are the precious qualities essential in the practice of Buddhism. On the other hand, guanxi plays a dominant role in any successful business venture in Chinese market. This paper develops the understanding of the two ancient bodies of knowledge of Buddhism and Confucianism. The attempt will be made in this paper to understand the deeper meaning of compassion in Buddhism in terms of compassionate virtues such as generosity, discipline, patience, diligence, humility, and wisdom. In guanxi, it will delve into different aspects of guanxi in terms of its background, meaning, significance, different types, intermediary, comparison with networking and ethics and success factors. This paper is based on the literature review on compassion--guanxi. The compassion literature focuses on books on Buddhism and commentaries by great Tibetan Buddhist sages. The guanxi literatures are predominately based on business research papers related to guanxi and business culture in China. The paper provides deeper meaning of compassion in Buddhism. The application of compassion to manage guanxi in China is the topic of this paper. This research is about optimizing the art of guanxi through the application of compassion which will help many non-Chinese business managers to effectively manage it. This paper is considered the first of its kind to study compassion in Buddhism and guanxi practice in China and seeks to provide the necessary framework to conduct the further research on applying compassion to build and manage better guanxi in China.

关键词： guanxi Buddhism compassion Chinese business culture China business strategy Confucius

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An Open-Source System for In Planta Gene Stacking by Bxbl and Cre Recombinases

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Molecular Plant 2014年第12期7卷 1756-1765页

作者： Lili Hou Yuan-Yeu Yau Junjie Wei Zhiguo Han Zhicheng Dong david W. Ow Plant Gene Engineering Center South China Agricultural Plant Molecular Analysis and Genetic Improvement Key Laboratory SouthChina Botanical Garden Chinese Academy of Sciences 723 Xingke Road Guangzhou 510650 China University of Chinese Academy of Sciences 19 Yuquan Road Beijing 100049 China Former Affiliation Plant Gene Expression Center USDA-ARS & Plant & Microbial Biology University of California-Berkeley 800Buchanan St. Albany CA 94710 USA

The rapid development of crops with multiple transgenic traits arouses the need for an efficient system for creating stacked cultivars. Most major crops rely on classical breeding to introgress the transgene from a laboratory variety to the numerous cultivars adapted to different growing regions. Even with vegetative propagated crops, genetic crosses are conducted during varietal improvement prior to vegetative cloning. The probability to assort the ＇x＇ number of transgenic loci into a single genome may seem trivial, （~）x for a diploid species, but given the ＇y＇ number of other nontransgenic traits that breeders also need to assemble into the same genome, the （~）~＊y probability for a ＇breeding stack＇ could quickly make the line conversion process unmanageable. Adding new transgenes onto existing transgenic varieties without creating a new segregating locus would require site-specific integration of new DNA at the existing transgenic locus. Here, we tested a recombinase-mediated gene-stacking scheme in tobacco. Sequential site-specific inte- gration was mediated by the mycobacteriophage Bxbl integrase-catalyzed recombination between attP and attB sites. Transgenic DNA no longer needed after integration was excised by Cre recombinase-mediated recombination of Iox sites. Site-specific integration occurred in -10% of the integration events, with half of those events usable as substrates for a next round of gene stacking. Among the site-specific integrants, however, a third experienced gene silencing. Overall, precise structure and reproducible expression of the sequentially added triple traits were obtained at an overall rate of -3% of the transformed clones--a workable frequency for the development of commercial cultivars. Moreover, since nei- ther the Bxbl-att nor the Cre-lox system is under patent, there is freedom to operate,

关键词： transgenesis site-specific recombination integrase gene targeting transgene expression.

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