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Transcriptomic Profiling of Human Pluripotent Stem Cell-derived Retinal Pigment epithelium over Time

Genomics, Proteomics & Bioinformatics 2021年第2期19卷 223-242页

作者： grace e.lidgerwood Anne Senabouth Casey J.A.Smith-Anttila Vikkitharan Gnanasambandapillai Dominik C.Kaczorowski Daniela Amann-Zalcenstein erica L.Fletcher Shalin H.Naik Alex W.Hewitt Joseph e.Powell Alice Pebay Department of Anatomy and Neuroscience The University of MelbourneParkvilleVIC 3010Australia Department of Surgery The University of MelbourneParkvilleVIC 3010Australia Centre for eye Research Australia Royal Victorian Eye and Ear HospitalEast MelbourneVIC 3002Australia Garvan Weizmann Centre for Cellular Genomics Garvan Institute of Medical ResearchThe Kinghorn Cancer CentreDarlinghurstNSW 2010Australia Single Cell Open Research endeavour The Walter and Eliza Hall Institute of Medical ResearchParkvilleVIC 3052Australia Immunology Division The Walter and Eliza Hall Institute of Medical ResearchParkvilleVIC 3052Australia Department of Medical Biology The University of MelbourneParkvilleVIC 3010Australia School of Medicine Menzies Institute for Medical ResearchUniversity of TasmaniaHobartTAS 7005Australia UNSW Cellular Genomics Futures Institute School of Medical SciencesUniversity of New South WalesSydneyNSW 2052Australia

Human pluripotent stem cell(h PSC)-derived progenies are immature versions of cells,presenting a potential limitation to the accurate modelling of diseases associated with maturity or ***,it is important to characterise how closely cells used in culture resemble their native *** order to select appropriate time points of retinal pigment epithelium(RPe)cultures that reflect native counterparts,we characterised the transcriptomic profiles of the h PSC-derived RPe cells from 1-and 12-month *** differentiated the human embryonic stem cell line H9 into RPe cells,performed single-cell RNA-sequencing of a total of 16,576 cells to assess themolecular changes of the RPe cells across these two culture time *** results indicate the stability of the RPe transcriptomic signature,with no evidence of an epithelial–mesenchymal transition,and with the maturing populations of the RPe observed with time in *** of Gene Ontology pathways revealed that as the cultures age,RPe cells upregulate expression of genes involved in metal binding and antioxidant *** might reflect an increased ability to handle oxidative stress as cells *** with native human RPe data confirms a maturing transcriptional profile of RPe cells in *** results suggest that long-term in vitro culture of RPe cells allows the modelling of specific phenotypes observed in native mature *** work highlights the transcriptional landscape of h PSC-derived RPe cells as they age in culture,which provides a reference for native and patient samples to be benchmarked against.

关键词： Human embryonic stem cell Human pluripotent stem cell Retinal pigment epithelium Single-cell RNA sequencing Ageing

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employing electrochemically derived pH gradients for Lab-on-PCB protein preconcentration devices

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Microsystems & Nanoengineering 2024年第1期10卷 79-87页

作者： grace Maxted Pedro estrela Despina Moschou Department of electronic and electrical engineering University of BathBath BA27AYUK Centre for Bioengineering and Biomedical Technologies(CBio) University of BathBath BA27AYUK

Protein preconcentration is an essential sample preparation step for analysis in which the targeted proteins exist in low concentrations,such as bodily fluids,water,or ***,very few practical implementations of miniaturized protein preconcentration devices have been demonstrated in practice,and even fewer have been integrated with other microanalytical *** approaches rely heavily on additional chemicals and reagents and introduce complexity to the overall *** this paper,we propose a novel miniaturized isoelectric focusing-based protein preconcentration screening device based on electrochemically derived pH gradients rather than existing chemical reagent *** this way,we reduce the need for additional chemical reagents to zero while enabling device incorporation in a seamlessly integrated full protein analysis microsystem via Lab-on-PCB *** apply our previously presented Lab-on-PCB approach to quantitatively control the pH of a solution in the vicinity of planar electrodes using electrochemical acid generation through redox-active self-assembled *** presented device comprises a printed circuit board with an array of gold electrodes that were functionalized with 4-aminothiophenol;this formed a self-assembled monolayer that was electropolymerized to improve its electrochemical *** preconcentration was performed in two *** first was open and needed the use of a holder to suspend a well of fluid above the electrodes;the second used microfluidic channels to enclose small volumes of *** here are the resulting data for protein preconcentration in both these forms,with a quantitative concentration factor shown for the open form and qualitative proof shown for the microfluidic.

关键词： electrochemical proof qualitative

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Relating estimates of wood properties of birch to stem form, age and species

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Journal of Forestry Research 2024年第3期35卷 1-11页

作者： grace Jones Maria Ulan Mateusz Liziniewicz Johan Lindeberg Stergios Adamopoulos Teagasc Forestry Development Department Ashtown Research CentreDublin 15 D15DY05Ireland Research Institutes of Sweden(RISe) GoteborgSweden The Forest Research Institute of Sweden(Skogforsk) Ekebo 225026890SvalovSweden Department of Forestry and Wood Technology Linnaeus University35195VaxjoSweden Department of Forest Biomaterials and Technology Swedish University of Agricultural SciencesBox 700875007 UppsalaSweden

Birch has long suffered from a lack of active forest management,leading many researchers to use mate-rial without a detailed management *** collected from three birch(Betula pendula Roth,*** ehrh.)sites in southern Sweden were analyzed using regression analysis to detect any trends or differences in wood proper-ties that could be explained by stand history,tree age and stem *** sites were genetics trials established in the same *** of acoustic velocity(AV)from non-destructive testing(NDT)and predicted AV had a higher correlation if data was pooled across sites and other stem form factors were considered.A subsample of stems had radial profiles of X-ray wood density and ring width by year created,and wood density was related to ring number from the pith and ring *** seemed likely that wood density was negatively related to ring width for both birch *** models had slight improvements if site and species were included,but only the youngest site with trees at age 15 had both birch *** paper indicated that NDT values need to be considered separately,and any predictive models will likely be improved if they are specific to the site and birch species measured.

关键词： Acoustic velocity Non-destructive testing Predictive models Regression analysis Wood density

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Fluid-rock interaction experiments with andesite at 100℃ for potential carbon storage in geothermal reservoirs

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Deep Underground Science and engineering 2024年第3期3卷 369-382页

作者： grace e.Belshaw elisabeth Steer Yukun Ji Herwin Azis Benyamin Sapiie Bagus Muljadi Veerle Vandeginste School of Chemistry University of NottinghamNottinghamUK Geoenergy Research Centre University of NottinghamNottinghamUK British Geological Survey KeyworthUK State Key Laboratory of Intelligent Construction and Healthy Operation and Maintenance of Deep Underground engineering China University of Mining and TechnologyXuzhouChina PT Supreme energy JakartaIndonesia Faculty of earth Sciences and Technology Institut Teknologi BandungBandungIndonesia Faculty of engineering University of NottinghamNottinghamUK Department of Materials engineering KU LeuvenCampus BrugesBrugesBelgium

Geothermal energy extraction often results in the release of naturally occurring carbon dioxide(CO_(2))as a *** on carbon storage using volcanic rock types other than basalt under both acidic and elevated temperature conditions has been limited so *** study uses batch reactor experiments at 100℃ to investigate the dissolution of andesite rock samples obtained from an active geothermal reservoir in Sumatra(Indonesia).The samples are subjected to reactions with neutral-pH fluids and acidic fluids,mimicking the geochemical responses upon reinjection of geothermal fluids,either without or with dissolved acidic gases,*** elemental analysis reveals the release of Ca^(2+)ions into the fluids through the dissolution of *** overall dissolution rate of the rock samples is 2.4×10^(–11)to 4.2×10^(–11)mol/(m^(2)·s),based on the Si release during the initial 7 h of the *** dissolution rates are about two orders of magnitude lower than those reported for basaltic rocks under similar reaction *** study offers valuable insights into the potential utilization of andesite reservoirs for effective CO_(2) storage via mineralization.

关键词： andesite carbon sequestration geothermal reservoirs plagioclase dissolution

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eco-ableism and access circularity in natural building

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Frontiers of Architectural Research 2024年第2期13卷 235-248页

作者： grace Schleck Lola Ben-Alon Barnard College Department of Architecture New YorkUSA Columbia University Graduate School of Architecture Planningand Preservation(GSAPP)New YorkUSA

The climate crisis disproportionately impacts disabled people. Yet climate-related advocacy, planning, and policymaking often neglect to thoughtfully include disabled people. Responding to this gap, disabled and neurodivergent environmental activists coined the term eco-ableism to describe discrimination and silencing toward disabled and neurodivergent people (i.e., ableism) arising in environmental spaces (i.e., eco-ableism). Relatedly, building operations and construction practices contribute a significant percentage of global, energy-related CO_(2) emissions annually, which calls into question the relationships between the impending climate crisis, disability justice, and architecture. Climate-specific, natural building materials and methods present a potential pathway toward a more sustainable built future: low-carbon, locally sourced, minimally processed, and nontoxic materials. Despite a critical overlap, there is little published research on material access in the production phase and human access in the occupation phase of natural buildings. Applying eco-ableism and material circularity in an architectural framework, this research aims to investigate the gaps and possibilities of access, natural material applications, and resulting US natural buildings informed by scholarship in critical disability studies and semi-structured interviews with natural building professionals.

关键词： eco-ableism Critical disability studies Climate crisis and architecture Naturalmaterialsand methods

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An adaptive machine learning-based optimization method in the aerodynamic analysis of a finite wing under various cruise conditions

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Theoretical & Applied Mechanics Letters 2024年第1期14卷 27-34页

作者： Zilan Zhang Yu Ao Shaofan Li grace X.Gu Department of Mechanical engineering University of CaliforniaBerkeley 94720USA Department of engineering Peking UniversityBeijing 100871China Department of Civil and environmental engineering University of CaliforniaBerkeley 94720USA

Conventional wing aerodynamic optimization processes can be time-consuming and imprecise due to the complexity of versatile flight *** of existing literature has considered two-dimensional infinite airfoil optimization,while three-dimensional finite wing optimizations are subject to limited study because of high computational *** we create an adaptive optimization methodology built upon digitized wing shape deformation and deep learning algorithms,which enable the rapid formulation of finite wing designs for specific aerodynamic performance demands under different cruise *** methodology unfolds in three stages:radial basis function interpolated wing generation,collection of inputs from computational fluid dynamics simulations,and deep neural network that constructs the surrogate model for the optimal wing *** has been demonstrated that the proposed methodology can significantly reduce the computational cost of numerical *** also has the potential to optimize various aerial vehicles undergoing different mission environments,loading conditions,and safety requirements.

关键词： Aerodynamic optimization Computational fluid dynamics Radial basis function Finite wing Deep learning neural network

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Relative carcinogenicity of tacrolimus vs mycophenolate after solid organ transplantation and its implications for liver transplant care

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World Journal of Hepatology 2024年第4期16卷 650-660页

作者： Dorothy Liu Mark M Youssef Josephine A grace Marie Sinclair Department of Gastroenterology Austin HealthMelbourne 3084VictoriaAustralia Victorian Liver Transplant Unit Austin HealthMelbourne 3084VictoriaAustralia Department of Medicine University of MelbourneMelbourne 3084VictoriaAustralia

BACKGROUND De novo malignancy is a leading cause of late morbidity and mortality in liver transplant *** immunosuppression has been shown to contribute to post-transplant malignancy(PTM)*** is emerging evidence on the differential carcinogenic risk profile of individual immunosuppressive drugs,independent of the net effect of *** inhibitors such as tacrolimus may promote tumourigenesis,whereas mycophenolic acid(MPA),the active metabolite of mycophenolate mofetil,may limit tumour *** transplantation(LT)is relatively unique among solid organ transplantation in that immunosuppression monotherapy with either tacrolimus or MPA is often achievable,which makes careful consideration of the risk-benefit profile of these immunosuppression agents particularly relevant for this ***,there is limited clinical data on this subject in both LT and other solid organ transplant *** To investigate the relative carcinogenicity of tacrolimus and MPA in solid organ *** A literature search was conducted using MeDLINe and embase databases using the key terms“solid organ transplantation”,“tacrolimus”,“mycophenolic acid”,and“carcinogenicity”,in order to identify relevant articles published in english between 1st January 2002 to 11th August *** terms,synonyms and explosion of MeSH terms,Boolean operators and truncations were also utilised in the *** lists of retrieved articles were also reviewed to identify any additional *** duplicates,abstracts from 1230 records were screened by a single reviewer,whereby 31 records were reviewed in ***-text articles were assessed for eligibility based on pre-specified inclusion and exclusion *** A total of 6 studies were included in this *** studies were large population registries or cohort studies,which varied in transplant era,type of organ transplanted and immunosuppression protocol us

关键词： Immunosuppression Solid organ transplantation Liver transplantation Carcinogenicity Tacrolimus Mycophenolate

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Contraceptive behaviors and awareness in an urban slum community of south India

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Asian pacific Journal of Reproduction 2024年第4期13卷 160-168页

作者： Monica Albert Sekhar Shanthi edward Angeline grace Sushmitha G Shirley esther Priscilla Department of Community Medicine Sree Balaji Medical College and Hospital(SBMCH)ChennaiIndia

Objective:To assess the factors associated with knowledge,attitude and behavior of contraception use among urban slum population in ***:A cross-sectional study was conducted in the urban slum of Anakaputhur,*** couples between ages of 19-49 years and living in slum areas were taken up for the study by simple random *** women,postnatal and postmenopausal women were excluded from the *** collection was done using a pretested structured questionnaire focusing on details regarding contraception knowledge,attitude,and *** analysis was done using IBM SPSS version *** test like Chi square and odds ratio(OR)were used to identify association between knowledge,attitude,and behavior of contraception with the associated variables and enter method of logistic regression analysis was ***:Out of the 360 respondents,228 were females,with a mean age of(34±5)*** 43%demonstrated adequate knowledge of contraception use,and 91%were aware of at least one contraceptive ***,72%exhibited a positive attitude towards contraceptive ***,43%(155 individuals)did not utilize any contraceptive *** contraceptive users,intrauterine devices were the most commonly adopted,followed by barrier methods and female *** was significant association between knowledge of contraception and education(aOR 7.29,95%CI 2.93-18.10,Pe(aOR 2.04,95%CI 1.19-3.50,Peconomic class(aOR 3.66,95%CI 1.71-7.85,Pegarding attitude towards contraception use,education(aOR 8.54,95%CI 2.35-31.03,Pex(aOR 0.15,95%CI 0.06-0.37,Pe(aOR 0.53,95%CI 0.28-0.99,Peconomic class(aOR 4.42,95%CI 1.97-9.92,Per of children(aOR 10.04,95%CI 4.26-23.66,Pere the variables that had a significant ***:These findings underscore the imperative for enhanced d

关键词： Family planning Unmet need Sterilization Knowledge Practice Urban slum

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Robot-assisted uretero-enteric reimplantation for uretero-enteric anastomotic strictures following robot-assisted radical cystectomy: Surgical approach and outcomes over two decades

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Asian Journal of Urology 2024年第3期11卷 384-390页

作者： Abdul Wasay Mahmood grace Harrington Zhe Jing Qiang Li Ahmed A.Hussein Khurshid A.Guru Department of Urology Roswell Park Comprehensive Cancer CenterElm and Carlton StreetsBuffaloNew YorkNYUSA

Objective:We described the technique and outcomes of robot-assisted repair of uretero-enteric strictures(UeS)following robot-assisted radical cystectomy(RARC)and urinary ***:Retrospective review of our RARC database from November 2005 to August 2023 at Roswell Park Comprehensive Cancer center was *** who developed UeS and ultimately underwent robot-assisted uretero-enteric reimplantation(RUeR)were *** method was used to compute the cumulative incidence recurrence rate of UeS after RUeR.A multivariable regression model was used to identify variables associated with UeS ***:A total of 123(15%)out of 808 RARC patients developed UeS,of whom 52 underwent reimplantation(45 patients underwent RUeR[n=55 cases]and seven patients underwent open ureteroenteric reimplantation).The median time from RARC to UeS was 4.4(interquartile range 3.0e7.0)months,and the median time between UeS and RUeR was 5.2(interquartile range 3.2e8.9)*** 3-year recurrence rate after RUeR is about 29%.On multivariable analysis,longer hospital stay(hazard ratio 1.37,95%confidence interval 1.16e1.61,ped with recurrent UeS after ***:RUeR for UeS after RARC is feasible with durable outcomes although a notable subset of patients experienced postoperative complications and UeS recurrence.

关键词： Stricture Cystectomy Reimplantation Robot-assisted

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Acute Colonic Pseudo-Obstruction (ACPO): An expanding Colon with Unusual Risk Factors

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Open Journal of Internal Medicine 2024年第2期14卷 167-174页

作者： Kelly Schulte Alyson Terry grace Boyle Dmitriy Scherbak HCA Healthone Sky Ridge Medical Center Lone Tree Colorado USA

The distention of the colon without mechanical or anatomical obstruction, Acute Colonic Pseudo-obstruction (ACPO), is a common condition occurring in the critically ill. ACPO in the setting of an acute pulmonary embolism and embolic stroke is a rarity. A 76-year-old female with shortness of breath, left hemiparesis and right-sided paresthesias presented with acute pulmonary embolism and acute infarcts of the left caudate nucleus, thalamus and occipital lobe. Her hospitalization was complicated with persistent distention of the large bowel without dilation of the small bowel. empiric antibiotics were initiated without improvement and laboratory studies including Clostridium difficile were negative. She underwent nasogastric decompression and two decompressive colonoscopies with a resolution of her symptoms. This case illustrates an example of acute abdominal distension, without underlying etiology, in the setting of acute embolism of the pulmonary and cerebral vasculature. early identification and action with decompressive colonoscopy were key to preventing further bowel damage or rupture.

关键词： Acute Colonic Pseudo-Obstruction ACPO Ogilvie’s Syndrome Colonic Dilation Acute embolic Infarcts Cerebrovascular Accident Pulmonary embolism Pe

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