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Optical Sum Frequency Generation Image of Rice Grains

Journal of Biomaterials and Nanobiotechnology 2012年第2期3卷 286-291页

作者： Hongyan Li Yoshihiro Miyauchi Nguyen Anh Tuan goro mizutani Mikio Koyano Japan Advanced Institute of Science and Technology 1-1 Asahidai Nomi Ishikawa 923-1292 Japan

We observed optical sum frequency generation (SFG) images of cross-sections of glutinous rice grains, in order to test a possibility of the SFG microscopy as a tool for monitoring polysaccharide species in rice grains. The SFG response in the CH vibration range was the most intense in the crush cell layer at the edge of the endosperm adjacent to the embryo probably due to optical reflection and scattering effectby the rugged dielectric structure of the crush cell layer. The SFG spectra as a function of the infrared wavelength depended on the measurement position in the endosperm. The SFG results were compared with those by Raman and infrared spectroscopies for the same samples.

关键词： Optical Sum Frequency Generation Microscopy Oryzaglutinosa (Rice) Amylopectin Starch Crush Cell Layer Endosperm

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Optical Sum Frequency Generation Spectroscopy of Cracked Non-Glutinous Rice (Oryza sativa L.) Kernels

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Journal of Biomaterials and Nanobiotechnology 2016年第1期7卷 13-18页

作者： Wataru Kouyama Takahiro Nishida Khuat Thi Thu Hien goro mizutani Hiroshi Hasegawa Hiroaki Miyamura School of Materials Science Japan Advanced Institute of Science and Technology Nomi Japan School of Environmental Science University of Shiga Prefecture Hikone Japan Shiga Prefecture Agricultural Technology Promotion Center Omihachiman Japan

In order to study the correlation between the cracking of rice (Oryza sativa L.) kernels and the molecular structure of the amylopectin in them, we attempted optical sum frequency generation (SFG) spectroscopy in the C-H stretching vibration region for normal and cracked japonica non-glutinous rice kernels. The samples were Koshihikari and Nipponbare. In Nipponbare, the width of the SFG spectrum peak at 2915 cm- 1 of the cracked rice kernels was broader than that of the normal ones, while for Koshihikari there was no clear difference. The width of the 2915 cm- 1 peak is suggested to originate from the variety of the higher-order structure of the saccharide chains in amylopectin. Although this is a tentative result, this method is shown to have a potential of serving for preventing the cracking of the rice kernels.

关键词： Rice Kernels Cracking in Hot Weather Optical Sum Frequency Generation (SFG) Amylopectin Non-Glutinous Rice

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