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Detection of plasmid-mediated IMP-1 metallo-β-lactamase and quinolone resistance determinants in an ertapenem-resistant Enterobacter cloacae isolate

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第5期10卷 348-354页

作者： Li-rong chen Hong-wei ZHOU Jia-chang CAI Rong ZHANG gong-xiang chen Clinical Laboratory Center the Second Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310009 China Cancer Institute Zhejiang University Hangzhou 310009 China

Objective： To investigate the mechanism of carbapenem resistance and the occurrence ofplasmid-mediated quinolone resistance determinants qnr and aac（6＇）-Ib-cr in a clinical isolate of Enterobacter cloacae. Methods： An ertapenem-resistant E. cloacae ZY106, which was isolated from liquor puris of a female gastric cancer patient in a Chinese hospital, was investigated. Antibiotic susceptibilities were determined by agar dilution method. Conjugation experiments, isoelectric focusing, polymerase chain reaction （PCR）, and DNA sequence analyses of plasmid-mediated carbapenemases and quinolone resistance determinants were preformed to confirm the genotype. Outer membrane proteins （OMPs） were examined by urea-sodium dodecyl sulfate- polyacrylamide gel electrophoresis （Urea-SDS-PAGE）. Results： Minimum inhibitory concentrations （MICs） of imipenem, mer- openem, and ertapenem for ZY 106 were 2, 4, and 16 pg/ml, respectively. Conjugation studies with Escherichia coli resulted in the transfer of significantly reduced carbapenem susceptibility. ZY106 produced IMP-1 metallo-β-lactamase and CTX-M-3 extended-spectrum β-1actamase, and E. coli transconjugant produced IMP-1. Plasrnid-mediated quinolone resistance determinant qnrS1 was detected in ZY106. Transfer of the qnrSl-encoding-plasmid into E. coli by conjugation resulted in intermediate resistance to ciprofloxacin in E. coli transconjugant. Urea-SDS-PAGE analysis of OMPs showed that ZYI06 lacked an OMP of approximately 38 kDa. Conclusion： It is the first IMP-l-producing Enterobacteriaceae in China and the first report of a clinical isolate that harbors both blalMP and qnrS genes as well. The blalMP-1, blaCTX-M-3, and qnrS1 are encoded at three different plasmids. IMP-1 combined with the loss of an OMP possibly resulted in ertapenem resistance and reduced imipenem and mero- penern susceptibility in E. cloacae.

关键词： Antibiotic resistance Carbapenem Enterobacteriaceae Outer membrane proteins (OMPs)

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Multi-source geospatial information integration and sharing in Virtual Globes

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Science China(Technological Sciences) 2010年第S1期53卷 1-6页

作者： gong JianYa,xiang LongGang,chen Jing & YUE Peng State Key Laboratory of Information Engineering in Surveying,Mapping and Remote Sensing,Wuhan University,Wuhan 430072,China Wuhan Univ State Key Lab Informat Engn Surveying Mapping & R Wuhan 430072 Peoples R China

Massive volumes of global geospatial information are being collected by newly designed acquisition devices and generated by various geospatial *** poses a critical challenge to efficiently organize and effectively integrate global multi-source geospatial *** paper introduces a global seamless data model for the organization of huge multi-source,multi-resolution and multi-temporal geospatial *** on this data model,the management of the global multi-resolution pyramid is discussed in *** integrate geospatial data and processing functions from various GIS systems,a service-oriented framework is presented through the technology of Virtual ***,a Virtual Globe system,GeoGlobe,is developed and illustrated.

关键词： Virtual Globe geospatial information multi-resolution pyramid Web service GeoGlobe

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Analysis-enhanced virtual globe for digital earth

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Science China(Technological Sciences) 2010年第S1期53卷 61-67页

作者： YUE Peng,gong JianYa,xiang LongGang & chen Jing State Key Laboratory of Information Engineering in Surveying,Mapping and Remote Sensing (LIESMARS),Wuhan University,Wuhan 430079,China 1. State Key Laboratory of Information Engineering in Surveying Mapping and Remote Sensing (LIESMARS) Wuhan University Wuhan 430079 China

Virtual globe has been recognized as an appealing technology to support the digital *** provides an intuitive and appealing way to visualize geospatial information,and is being used frequently to bring the globe-scale geospatial information to our *** easy access to virtual globes such as Google Earth,Microsoft Virtual Earth,and NASA World Wind allows both the general public users and professionals to use them for educational and research *** virtual globes provide a number of functions for geospatial data visualization and annotation,the geospatial analysis functions available in the current virtual globes are *** paper addresses an approach to the establishment of an analysis-enhanced virtual *** approach is based on the integration of virtual globe and interoperable geoprocessing *** an analysis-enhanced virtual globe combines the visualization and communication power of virtual globes with the conventional powerful analysis functions of GIS,thus meeting the analysis demands in digital earth.

关键词： virtual globe digital earth geoprocessing service GIS GeoGlobe GeoPW

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A novel broth enrichment method for screening of Carbapenem-Resistant Enterobacteriaceae from stool samples

A novel broth enrichment method for screening of Carbapenem-...

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2019年浙江省医学会医学微生物与免疫学分会学术大会

作者： Danxia Gu gong-xiang chen Rong Zhang Centre of Laboratory Medicine Zhejiang Provincial People's HospitalPeople's Hospital of Hangzhou Medical College Department of Clinical Laboratory Medicine Second Affiliated Hospital of Zhejiang UniversitySchool of MedicineZhejiang University

Objective:To evaluate a novel broth enrichment method for CRE screening from stool ***:A comprehensive comparison was conducted between In-house method and four other methods,including broth enrichment method recommended by CDC(CDC1),modified broth enrichment method(CDC2),direct disks screening method(MacD),and MacConkey agar with imipenem at 1 ug/ml(MacI).Direct detection of carbapenem resistant genes from stool samples after broth enrichment process by PCR and sequencing was referred to as gold *** clinical samples which contained 15 well-characterized CRE strains were subjected to analysis of the limit of detection(LOD) of CRE by distinct ***,442 non-duplicated clinical stool samples were collected and tested for performance of different *** of species was performed by matrix assisted laser desorption ionization-time of flight mass spectrometry(MALDI-TOF MS),and antimicrobial susceptibility testing was performed by micro-broth dilution ***:With simulated stool samples containing known CRE strains,In-house method detected all of the strains with the lowest limits of detection(LODs) ranging from 101-105 CFU/*** CDC1 method,13/15 of the simulated samples were correctly identified,and the LODs spans between 103-108 CFU/***2 method identified12/15 of the samples with LODs ranging from 104-108 CFU/*** for MacD,14/15 of the samples were identified with LODs of 105-108 CFU/*** method identified all of the 15 samples with LODs of 104-108 CFU/*** for the 442 clinical stool samples,22(4.98%) CRE strains were identified including ***(n=6),***(n=5),Klebsiella oxytoca(n=3),Citrobacter freundii(n=3),Enterobacter aerogenes(n=2),Halfnia alvei(n=2) and Klebsiella ornithinolytica(n=1).Among the identified CRE strains,the MIC50 and MIC90,respectively,were as follows:imipenem,16 μg/ml,64 μg/ml;meropenem,16 μg/ml,128 μg/ml;ertapenem,32 μg/ml,>128 μg/*** which,72.73%(16/22) carried blaN

关键词： carbapenem-resistant Enterobacteriaceae novel method screening sensitivity limit of detection interference

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A novel broth enrichment method for screening of Carbapenem-Resistant Enterobacteriaceae from stool samples

A novel broth enrichment method for screening of Carbapenem-...

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2019年浙江省检验医学学术大会

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Potassium and calcium channels in different nerve cells act as therapeutic targets in neurological disorders

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Neural Regeneration Research 2025年第5期20卷 1258-1276页

作者： Qing Qiu Mengting Yang Danfeng gong Haiying Liang Tingting chen Department of Pharmacology School of PharmacyNantong UniversityNantongJiangsu ProvinceChina Jiangsu Province Key Laboratory of Inflammation and Molecular Drug Target NantongJiangsu ProvinceChina Department of Pharmacy Longyan First Affiliated Hospital of Fujian Medical UniversityLongyanFujian ProvinceChina

The central nervous system, information integration center of the body, is mainly composed of neurons and glial cells. The neuron is one of the most basic and important structural and functional units of the central nervous system, with sensory stimulation and excitation conduction functions. Astrocytes and microglia belong to the glial cell family, which is the main source of cytokines and represents the main defense system of the central nervous system. Nerve cells undergo neurotransmission or gliotransmission, which regulates neuronal activity via the ion channels, receptors, or transporters expressed on nerve cell membranes. Ion channels, composed of large transmembrane proteins, play crucial roles in maintaining nerve cell homeostasis. These channels are also important for control of the membrane potential and in the secretion of neurotransmitters. A variety of cellular functions and life activities, including functional regulation of the central nervous system, the generation and conduction of nerve excitation, the occurrence of receptor potential, heart pulsation, smooth muscle peristalsis, skeletal muscle contraction, and hormone secretion, are closely related to ion channels associated with passive transmembrane transport. Two types of ion channels in the central nervous system, potassium channels and calcium channels, are closely related to various neurological disorders, including Alzheimer's disease, Parkinson's disease, and epilepsy. Accordingly, various drugs that can affect these ion channels have been explored deeply to provide new directions for the treatment of these neurological disorders. In this review, we focus on the functions of potassium and calcium ion channels in different nerve cells and their involvement in neurological disorders such as Parkinson's disease, Alzheimer's disease, depression, epilepsy, autism, and rare disorders. We also describe several clinical drugs that target potassium or calcium channels in nerve cells and could be u

关键词： astrocytes calcium channels central nervous system extracellular ion concentration microglia neurological disorders neurons potassium channels

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Engine Misfire Fault Detection Based on the Channel Attention Convolutional Model

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Computers, Materials & Continua 2025年第1期82卷 843-862页

作者： Feifei Yu Yongxian Huang Guoyan chen Xiaoqing Yang Canyi Du Yongkang gong School of Mechatronic Engineering Guangdong Polytechnic Normal UniversityGuangzhou510665China School of Automotive and Transportation Engineering Guangdong Polytechnic Normal UniversityGuangzhou510665China

To accurately diagnosemisfire faults in automotive engines,we propose a Channel Attention Convolutional Model,specifically the Squeeze-and-Excitation Networks(SENET),for classifying engine vibration signals and precisely pinpointing misfire *** the experiment,we established a total of 11 distinct states,encompassing the engine’s normal state,single-cylinder misfire faults,and dual-cylinder misfire faults for different *** collection was facilitated by a highly sensitive acceleration signal collector with a high sampling rate of 20,*** collected data were methodically divided into training and testing sets based on different experimental groups to ensure generalization and prevent overlap between the two *** results revealed that,with a vibration acceleration sequence of 1000 time steps(approximately 50 ms)as input,the SENET model achieved a misfire fault detection accuracy of 99.8%.For comparison,we also trained and tested several commonly used models,including Long Short-Term Memory(LSTM),Transformer,and Multi-Scale Residual Networks(MSRESNET),yielding accuracy rates of 84%,79%,and 95%,*** underscores the superior accuracy of the SENET model in detecting engine misfire faults compared to other ***,the F1 scores for each type of recognition in the SENET model surpassed 0.98,outperforming the baseline *** analysis indicated that the misclassified samples in the LSTM and Transformer models’predictions were primarily due to intra-class misidentifications between single-cylinder and dual-cylinder misfire *** delve deeper,we conducted a visual analysis of the features extracted by the LSTM and SENET models using T-distributed Stochastic Neighbor Embedding(T-SNE)*** findings revealed that,in the LSTMmodel,data points of the same type tended to cluster together with significant ***,in the SENET model,data points of various types were more widely and evenly dispersed,demonstra

关键词： Channel attention SENET model engine misfire fault fault detection

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Characteristic changes in astrocyte properties during astrocyte-to-neuron conversion induced by NeuroD1/Ascl1/Dlx2

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Neural Regeneration Research 2025年第6期20卷 1801-1815页

作者： Qing He Zhen Wang Yuchen Wang Mengjie Zhu Zhile Liang Kanghong Zhang Yuge Xu gong chen GHM Institute of CNS Regeneration Jinan UniversityGuangzhouGuangdong ProvinceChina

Direct in vivo conversion of astrocytes into functional new neurons induced by neural transcription factors has been recognized as a potential new therapeutic intervention for neural injury and degenerative disorders. However, a few recent studies have claimed that neural transcription factors cannot convert astrocytes into neurons, attributing the converted neurons to pre-existing neurons mis-expressing transgenes. In this study, we overexpressed three distinct neural transcription factors––NeuroD1, Ascl1, and Dlx2––in reactive astrocytes in mouse cortices subjected to stab injury, resulting in a series of significant changes in astrocyte properties. Initially, the three neural transcription factors were exclusively expressed in the nuclei of astrocytes. Over time, however, these astrocytes gradually adopted neuronal morphology, and the neural transcription factors was gradually observed in the nuclei of neuron-like cells instead of astrocytes. Furthermore,we noted that transcription factor-infected astrocytes showed a progressive decrease in the expression of astrocytic markers AQP4(astrocyte endfeet signal), CX43(gap junction signal), and S100β. Importantly, none of these changes could be attributed to transgene leakage into preexisting neurons. Therefore, our findings suggest that neural transcription factors such as NeuroD1, Ascl1, and Dlx2 can effectively convert reactive astrocytes into neurons in the adult mammalian brain.

关键词： aquaporin-4 Ascl1 astrocyte cortex Dlx2 gap junction glia-to-neuron conversion neural regeneration NeuroD1 reprogramming

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降雨条件下炭质泥岩-土分层路堤含水特征试验

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长安大学学报（自然科学版） 2016年第5期36卷 29-36页

作者：曾铃邱祥付宏渊周功科陈向阳长沙理工大学道路结构与材料交通行业重点实验室湖南长沙410076 长沙理工大学土木与建筑学院湖南长沙410004 长沙理工大学交通运输工程学院湖南长沙410004 现代公路交通基础设施先进建养技术湖南省协同创新中心湖南长沙410004

为研究降雨条件下炭质泥岩-土分层填筑路堤的含水特征,采用室内试验与数值模拟相结合的研究方法,开展人工模拟降雨的路堤物理模型试验,分析雨水入渗时路堤内部含水率的变化特征、暂态饱和区演化过程,并结合数值试验对试验结果进行了验... 详细信息

为研究降雨条件下炭质泥岩-土分层填筑路堤的含水特征,采用室内试验与数值模拟相结合的研究方法,开展人工模拟降雨的路堤物理模型试验,分析雨水入渗时路堤内部含水率的变化特征、暂态饱和区演化过程,并结合数值试验对试验结果进行了验证。研究结果表明:短时降雨对路堤表层土体含水率影响较小,随着降雨的持续,其影响程度增大;受填筑形式的影响,各截面含水率分布曲线呈"S"形或倒"S"形;路堤边坡坡面以下同一深度处土体含水率增大的幅度和速率与其高程呈反比,路堤内部同一高程处土体含水率增大的幅度和速率与其距坡面的距离呈反比;降雨入渗形成的暂态饱和区由坡面向路堤内部整体扩展,其上部扩展速度小于路堤下部,同时在预崩解炭质泥岩中的扩展速度快于土中的速度,当暂态饱和区扩展到一定深度后将引起地下水位的升高;模型试验人眼观察的局限性、填筑过程中水分的迁移及尺寸效应,使得模型试验与数值试验结果之间存在一定差异;路堤特征点、截面含水率变化特征以及暂态饱和区分布形式的研究试验成果,对开展炭质泥岩-土分层填筑路堤在降雨条件下的后续崩解机理及失稳模式研究具有参考意义。

关键词：道路工程炭质泥岩降雨条件下模型试验含水特征

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基于CPU_MIC_GPU异构架构的Roberts算法优化探究

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科学技术与工程 2016年第36期16卷 217-222页

作者：洪向共陈威熊镝南昌大学南昌330031

在高分辨率图像日益普及的情况下,Roberts边缘检测的处理速度急需进一步提高。在CPU表现不尽如人意的情况下,基于CPU/GPU和CPU/MIC的高度并行运算的研究愈加深入。在分析Roberts算法特点的基础上,将能并行的部分移植到GPU和MIC上进行。... 详细信息

在高分辨率图像日益普及的情况下,Roberts边缘检测的处理速度急需进一步提高。在CPU表现不尽如人意的情况下,基于CPU/GPU和CPU/MIC的高度并行运算的研究愈加深入。在分析Roberts算法特点的基础上,将能并行的部分移植到GPU和MIC上进行。完成基于CPU/GPU和CPU/MIC的异构架构上的Roberts算法实现,并针对CPU/MIC上将程序进行向量化优化。实验结果表明,在相同单精度浮点运算能力下,GPU处理低分辨率图像的速度更快、加速比更高,但处理高分辨率图像时MIC的加速比最高为23.52,高于GPU的21.43。

关键词： MIC GPU Roberts边缘检测 Gauss滤波并行运算图像处理

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