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酸激发剂对固废材料活性激发效果的影响

硅酸盐通报 2024年第8期43卷 2941-2951页

作者：张会芳龚琳洋陈洁张玉栋曹慧刘哲颖李玉宽魏文博刘凯宏河北建筑工程学院土木工程学院张家口075000 河北省装配式建筑与地下工程技术创新中心张家口075000 张家口市住房和城乡建设局张家口075000 张家口市桥西区市政工程和地方道路服务中心张家口075000 河北智诚检验认证集团有限公司张家口075000

为了探究酸激发剂种类和浓度对固体废弃物的活性激发效果,采用控制变量法,选用三种酸溶液作为激发剂对粉煤灰和矿渣进行活性激发,激发后的粉煤灰和矿渣混合作为胶凝材料取代30%(质量分数,下同)的水泥,选用全铁尾矿砂和全再生骨料两种材... 详细信息

为了探究酸激发剂种类和浓度对固体废弃物的活性激发效果,采用控制变量法,选用三种酸溶液作为激发剂对粉煤灰和矿渣进行活性激发,激发后的粉煤灰和矿渣混合作为胶凝材料取代30%(质量分数,下同)的水泥,选用全铁尾矿砂和全再生骨料两种材料分别作为灌浆料试件的两种细骨料进行试验。试验结果表明:经适量浓度的酸激发剂浸泡激发的固废材料活性得到不同程度的提升;对比全铁尾矿砂试验组和全再生细骨料试验组的强度发现酸激发剂对再生骨料试验组的强度提升效果较好;对于同种细骨料的试验组,盐酸对试件强度提升效果较好。当酸溶液种类相同时,浓度为1%的草酸溶液、1%的醋酸溶液、2%的盐酸溶液对铁尾矿砂试验组的提升效果较好;浓度为1%的草酸溶液、3%的醋酸溶液、1.5%的盐酸溶液的再生骨料试验组提升效果较好。通过X射线衍射(XRD)和扫描电子显微镜(SEM)分析发现使用酸激发胶凝材料,试件中石英和钙矾石相对含量上升,说明酸激发能够促进水化反应的进行。

关键词：酸激发固体废弃物水泥基灌浆料铁尾矿砂再生细骨料力学性能

ICH-induced inflammatory response upregulats hepcidin expression inhibits brain iron clearance and its mechanism

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ICH-induced inflammatory response upregulats hepcidin expres...

成纤维细胞胶原合成及细胞内STAT6蛋白磷酸化与白细胞介素13的促进作用

中国神经科学学会第十二届全国学术会议

作者： Xiong xy Liu L Wang FX Yang YR Hao JW Wang PF Zhong Q Zhou K Xiong A Zhu WY Zhao T Meng ZY Wang YC gong QW Liao MF Wang J Yang QW Department of Neurology Xinqiao Hospital Third Military Medical University Department of Neurology Key Laboratory of Neurorepair and Regeneration Tianjin and Ministry of Education Tianjin Neurological Institute Tianjin Medical University General Hospital Department of Neurology Weihai Municipal Hospital Department of Anesthesiology/Critical Care Medicine School of Medicine Johns Hopkins University

Objective Reducing the deposited brain iron is one of important measures in treatment of intracerebral hemorrhage（ICH）. Currently, as the mechanism of brain iron metabolism and its regulating and controlling factors after ICH are not clear, the effect of the treatment of reducing iron simply by physical method has larger difference. So exploring the mechanism of brain iron metabolism following ICH and giving the targeted intervention have important roles in the ICH therapy. Methods Parabiosis and ICH models combined with in vivo and in vitro experiments were used to investigate the roles of hepcidin in brain iron metabolism after ICH. Results After ICH, hepcidin-25 was found in serum and primarily in astrocytes. As human hepcidin-25 peptide injected into WT ICH mice, which aggravated the brain iron efflux, oxidative brain injury, and cognition impairment, but not in Hepc-/-ICH mice. Data obtained in in vitro studies showed that increased hepcidin inhibited the intracellular iron efflux of brain microvascular endothelial cells but was rescued by a hepcidin antagonist, fursultiamine. Using parabiosis ICH models also shows that increased serum hepcidin prevents brain iron efflux. In addition, Toll-like receptor 4（TLR4）/My D88 signaling pathway increased hepcidin expression by promoting interleukin-6 expression and signal transducer and activator of transcription 3 phosphorylation. TLR4-/-and My D88-/-mice exhibited improvement in brain iron efflux at 7, 14, and 28 days after ICH, and the TLR4 antagonist（6 R）-6-[N-（2-chloro-4-fluorophenyl） sulfamoyl] cyclohex-1-ene-1-carboxylate significantly decreased brain iron levels at days 14 and 28 after ICH and improved cognition impairment at day 28. Conclusion This study further elucidate the mechanism of brain iron metabolism after ICH, and found TLR4 inhibitors could improve the effect of ICH treatment via inhibiting the hepcidin expression to reduce the deposited brain *** is expected to provide new methods for the tre

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中国组织工程研究与临床康复 2007年第2期11卷 275-277,281页

作者：熊丽霞石小玉李文林周莹龚妍春南昌大学基础医学院病理生理学教研室江西省南昌市330006 南昌大学基础医学院组织胚胎学教研室江西省南昌市330006 江西医学科学研究所江西省医学生物高技术重点实验室江西省南昌市330006

目的:观察重组白细胞介素13对成纤维细胞3T3的胶原合成作用,及其在3T3细胞胞内的信号转导途径,探讨白细胞介素13促纤维化的作用机制。方法:实验于2005-03/2006-03在江西医学科学研究所江西省医学生物高技术重点实验室进行。将3T3细胞分... 详细信息

目的:观察重组白细胞介素13对成纤维细胞3T3的胶原合成作用,及其在3T3细胞胞内的信号转导途径,探讨白细胞介素13促纤维化的作用机制。方法:实验于2005-03/2006-03在江西医学科学研究所江西省医学生物高技术重点实验室进行。将3T3细胞分为实验组和对照组,在进行实验前,两组细胞均用无血清DMEM培养12～16h,实验组加重组白细胞介素13(100 μg/L),作用24,48,72h后,应用胶原纤维特殊染色观察白细胞介素13作用下3T3细胞体外合成胶原纤维情况;上清液用羟脯氨酸试验检测细胞分泌的总胶原含量;用逆转录-聚合酶链反应检测3T3细胞白细胞介素13受体的表达;用免疫印迹技术检测白细胞介素13作用0,1,2,4h后STAT6蛋白磷酸化情况,组间比较采用t检验。结果:①实验组细胞胶原纤维合成增加及上清液分泌总胶原含量高于对照组(P<0.05)。②逆转录-聚合酶链反应检测到3T3细胞表达白细胞介素13受体α1。③免疫印迹技术检测到白细胞介素13作用1,2,4h均有磷酸化STAT6蛋白表达,且白细胞介素13作用2h磷酸化STAT6蛋白表达最强,4h衰减。结论:白细胞介素13受体α1在3T3细胞上表达,重组白细胞介素13促进3T3细胞胶原合成和STAT6蛋白磷酸化,可能在纤维化发病机制中发挥重要作用。

关键词：白细胞介素13 成纤维细胞白细胞介素受体转录激活转录因子

Origin and chromatin remodeling of young X/Y sex chromosomes in catfish with sexual plasticity

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National Science Review 2023年第2期10卷 91-106页

作者： Gaorui gong Yang Xiong Shijun Xiao Xi-Yin Li Peipei Huang Qian Liao Qingqing Han Qiaohong Lin Cheng Dan Li Zhou Fan Ren Qi Zhou Jian-Fang Gui Jie Mei Hubei Hongshan Laboratory College of Fisheries Huazhong Agricultural University Jiaxing Key Laboratory for New Germplasm Breeding of Economic Mycology State Key Laboratory of Freshwater Ecology and Biotechnology Hubei Hongshan Laboratory Institute of HydrobiologyChinese Academy of Sciences University of the Chinese Academy of Sciences School of Animal Science and Nutritional Engineering Wuhan Polytechnic University MOE Laboratory of Biosystems Homeostasis & Protection Life Sciences InstituteZhejiang University

Assembly of a complete Y chromosome is a significant challenge in animals with an XX/xy sex-determination *** cently,we created YY-supermale yellow catfish by crossing xy males with sex-reversed xy females,providing a valuable model for Y-chromosome assembly and ***,we assembled highly homomorphic Y and X chromosomes by sequencing genomes of the YY supermale and XX female in yellow catfish,reve aling their nucleotide divergences with only less than 1% and with the same gene *** sex-determining region(SDR) was identified to locate within a physical distance of0.3 Mb by ***,the incipient sex chromosomes were revealed to originate via autosome-autosome fusion and were characterized by a highly rearranged region with an SDR downstream of the fusion *** found that the Y chromosome was at a very early stage of differentiation,as no clear evidence of evolutionary strata and classical structure features of recombination suppression for a rather late stage of Y-chromosome evolution were ***,a number of sex-antagonistic mutations and the accumulation of repetitive elements were discovered in the SDR,which might be the main driver of the initial establishment of recombination suppression between young X and Y ***,distinct three-dimensional chromatin organizations of the Y and X chromosomes were identified in the YY supermales and XX females,as the X chromosome exhibited denser chromatin structure than the Y chromosome,while they respectively have significantly spatial interactions with female-and male-related genes compared with other *** chromatin configuration of the sex chromosomes as well as the nucleus spatial organization of the XX neomale were remodeled after sex reversal and similar to those in YY supermales,and a male-specific loop containing the SDR was found in the open chromatin *** results elucidate the origin of young sex chromosomes and the chromatin remodel

关键词： Y chromosome sex-chromosome evolution chromosome fusion chromatin organization sexual reversal

州市级动物疫控机构兽用生物制品储运管理应注意的问题

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中国兽药杂志 2009年第9期43卷 52-54页

作者：濮永华杨兴余龚兴建保山市动物疫病预防控制中心云南保山678000

从实际工作需要出发,以保山市动物疫病预防控制中心的工作实践为基础,本文较系统地分析总结了州市级动物疫控机构兽用生物制品储运管理应注意的问题。它具有较强的可操作性和实用性,对保证疫苗质量、提高免疫效果至关重要,为州市级动物... 详细信息

从实际工作需要出发,以保山市动物疫病预防控制中心的工作实践为基础,本文较系统地分析总结了州市级动物疫控机构兽用生物制品储运管理应注意的问题。它具有较强的可操作性和实用性,对保证疫苗质量、提高免疫效果至关重要,为州市级动物疫控机构加强兽用生物制品储运管理和从事该项工作但缺乏工作经验的专业人员提供了可操作的参考意见。

关键词：动物疫病预防兽用生物制品储运管理机构市级可操作性控制中心疫苗质量

外周血核型45,XO表型为男性性发育异常一例

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中华儿科杂志 2020年第3期58卷 243-244页

作者：秦淼巩纯秀国家儿童医学中心首都医科大学附属北京儿童医院内分泌代谢科100045

1例1岁9月龄患儿,社会性别男,以生后外阴异常为主诉,体格检查发现患儿尿道开口于会阴,伴有阴茎下弯,阴茎阴囊转位,左侧阴囊内可及睾丸,右侧阴囊及腹股沟区未触及似睾丸包块,但多次查外周血染色体核型为45,XO未检测到SRY基因,与临床表型... 详细信息

1例1岁9月龄患儿,社会性别男,以生后外阴异常为主诉,体格检查发现患儿尿道开口于会阴,伴有阴茎下弯,阴茎阴囊转位,左侧阴囊内可及睾丸,右侧阴囊及腹股沟区未触及似睾丸包块,但多次查外周血染色体核型为45,XO未检测到SRY基因,与临床表型不符。进一步行口腔黏膜、包皮组织及性腺组织FISH检测,发现为45,XO/46,xy/46,X ish der(Y)t(Y;Y)嵌合体。外周血染色体核型为45,XO,临床表型为男性的情况非常少见。

关键词：外周血染色体右侧阴囊临床表型左侧阴囊尿道开口腹股沟区性发育异常 SRY基因

Variant analysis of the chromodomain helicase dNA-binding protein 7 in pediatric disorders of sex development

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Pediatric Investigation 2019年第1期3卷 31-38页

作者： Beibei Zhang Yanning Song Wei Li Chunxiu gong National Center for Children’s Health BeijingChina Center of Endocrinology Genetics and MetabolismBeijing Children’s HospitalCapital Medical UniversityBeijingChina Beijing Key Laboratory for Genetics of Birth Defects BeijingChina Beijing Pediatric Research Institute BeijingChina

Importance:This study investigated the role of the chromodomain helicase DNA-binding protein 7(CHD7)in disorders of sex development(DSD).Objective:We aimed to present the potential pathogenicity of CHD7 variants in pediatric patients with ***:Choosing cases with CHD7 variants from DSD patients in Beijing Children’s Hospital to assess for the *** software tools were used to predict variant pathogenicity in these ***:Among the 113 DSD patients,22 cases had CHD7 ***-four different CHD7 variants were identified in the 22 DSD *** software combined with ClinVar database information and their clinical manifestations revealed that,of the 18 patients with 46,xy DSD,two had CHARGE syndrome and two had Kallmann *** of the variants were highly categorized as“likely to be pathogenic”and seven as“suspected to be pathogenic”.Of the four patients with 46,XX DSD,three had ovotesticular DSD(c.305A>G,c.2788G>A,and c.3098G>A)and one had testicular DSD(c.2831G>A).Interpretation:A high frequency of CHD7 variants was found in the DSD patients,especially those with 46,xy ***,the detection of a pathogenic CHD7 variant could suggest a diagnosis of hypogonadotropic hypogonadism for 46,xy DSD patients,but pre-pubescent patients should be reassessed in adolescence to confirm this *** study also suggests that DNA sequencing could help to identify pre-pubescent DSD *** data are required to determine the connection between CHD7 variants and sex-reversal in patients with 46,XX DSD,and the accumulation of these data is essential and necessary for DSD research.

关键词： Disorders of sex development CHD7 variants Genital abnormalities

面神经损伤后面神经核中黏附分子CD44表达与穴位电针刺激的影响

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中国组织工程研究与临床康复 2007年第27期11卷 5369-5373页

作者：李雷激骆文龙伍宗惠欧小毅杨利龚林杨朝鲜袁琼兰泸州医学院附属医院耳鼻咽喉头颈外科四川省泸州市646000 重庆医科大学第二临床学院耳鼻咽喉头颈外科重庆市410010 泸州医学院附属医院妇产科四川省泸州市646000 泸州医学院附属医院组织胚胎学教研室四川省泸州市646000 泸州医学院附属医院神经生物学实验室四川省泸州市646000

目的:观察穴位电针在面神经损伤后面神经再生中的作用及对黏附分子CD44表达的影响。方法:实验于2005-09/2006-02在泸州医学院神经生物学实验室完成。①实验材料:普通级6～8个月龄新西兰家兔36只,体质量2～2.75kg,雌雄不拘。②实验方法:... 详细信息

目的:观察穴位电针在面神经损伤后面神经再生中的作用及对黏附分子CD44表达的影响。方法:实验于2005-09/2006-02在泸州医学院神经生物学实验室完成。①实验材料:普通级6～8个月龄新西兰家兔36只,体质量2～2.75kg,雌雄不拘。②实验方法:采用随机数字法取兔32只,压榨损伤右侧面神经上颊支制备面神经损伤模型。③实验分组:随机分为手术对照组16只,不作穴位电针刺激,自然恢复;针刺组16只术后立即行翳风、颧髎、颊车、地仓、阳白、四白及合谷穴位电针治疗,1次/d,30min/次;另取4只家兔做正常对照组。④实验评估:于术后1,4,7,14d每组分别取4只兔麻醉后,经心灌注处死动物,苏木精-伊红染色观察面神经核的改变,免疫组织化学观察CD44的表达。结果:36只兔全部进入结果分析。①手术对照组和针刺组兔面神经核运动神经元的形态学改变:术后1d,两组细胞形态正常;术后4d,两组细胞肿胀、胞浆空泡化、染色质溶解、核仁偏移、尼氏小体消失;术后7d,手术对照组有明显神经元的死亡,针刺组仅有神经元变性表现,并出现核仁回归、尼氏小体重现;术后14d,手术对照组神经元的坏死更为明显,针刺组神经元肿胀及变性程度有明显改善。②各组CD44标记阳性面神经核运动神经元数目:正常组,面神经核中有CD44表达。术后1d,针刺组和手术对照组CD44有明显增加;术后4d,针刺组CD44阳性细胞数较手术对照组显著性增加(P<0.01);术后7d,手术对照组的CD44阳性细胞数目较针刺组增加(P<0.05)。术后14d,两组比较无差别,阳性细胞免疫染色着色不一。结论:穴位电刺激能促进黏附分子CD44在损伤面神经核的表达。

关键词：针刺学位面神经损伤抗原,CD44 组织构建

X连锁基因遗传平衡数学模型的建构及相关推论

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生物学教学 2019年第3期44卷 63-64页

作者：龚军辉河北省保定市第一中学保定071000

本文以xy染色体决定性别的种群为例,建构X连锁基因遗传平衡的数学模型,并提出几个有价值的推论。

关键词： X连锁基因遗传平衡模型建构