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AGK2 pre-treatment protects against thioacetamide-induced acute liver failure via regulating the MFN2-PERK axis and ferroptosis signaling pathway

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Hepatobiliary & Pancreatic Diseases International 2024年第1期23卷 43-51页

作者： Qing-Qi Zhang Qian Chen Pan Cao Chun-Xia Shi Lu-Yi Zhang Lu-wen Wang Zuo-Jiong gong Department of Infectious Diseases Renmin Hospital of Wuhan UniversityWuhan 430060China

Background:Acute liver failure(ALF)is an unpredictable and life-threatening critical *** pathological characteristic of ALF is massive necrosis of hepatocytes and lots of inflammatory cells infiltration which may lead to multiple organ ***:Animals were divided into 3 groups,normal,thioacetamide(TAA,ALF model)and TAA+*** L02 cells were divided into 5 groups,normal,TAA,TAA+mitofusin 2(MFN2)-siRNA,TAA+AGK2,and TAA+AGK2+MFN2-siRNA *** liver histology was evaluated with hematoxylin and eosin staining,inositol-requiring enzyme 1(IRE1),activating transcription factor 6β(ATF6β),protein kinase R(PKR)-like endoplasmic reticulum kinase(PERK)and phosphorylated-PERK(p-PERK).C/EBP homologous protein(CHOP),reactive oxygen species(ROS),MFN2 and glutathione peroxidase 4(GPX4)were measured with Western blotting,and cell viability and liver chemistry were also *** endoplasmic reticulum membranes(MAMs)were measured by ***:The liver tissue in the ALF group had massive inflammatory cell infiltration and hepatocytes necrosis,which were reduced by AGK2 *** comparison to the normal group,apoptosis rate and levels of IRE1,ATF6β,p-PERK,CHOP,ROS and Fe2+in the TAA-induced ALF model group were significantly increased,which were decreased by AGK2 *** levels of MFN2 and GPX4 were decreased in TAA-induced mice compared with the normal group,which were enhanced by AGK2 *** with the TAA-induced L02 cell,apoptosis rate and levels of IRE1,ATF6β,p-PERK,CHOP,ROS and Fe2+were further increased and levels of MFN2 and GPX4 were decreased in the MFN2-siRNA ***2 pre-treatment decreased the apoptosis rate and levels of IRE1,ATF6β,p-PERK,CHOP,ROS and Fe2+and enhanced the protein expression of MFN2 and GPX4 in MFN2-siRNA treated L02 *** observation showed that level of MAMs was promoted in the AGK2 pre-treatment group when compared with the TAA-induced gr

关键词： SIRT2 inhibitor AGK2 Acute liver failure MFN2 Ferroptosis

Gambogenic acid induces apoptosis via upregulation of Noxa in oral squamous cell carcinoma

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Chinese Journal of Natural Medicines 2024年第7期22卷 632-642页

作者： CHENG Xinran feng Mengyuan ZHANG Anjie GUO Jian gong Yunlai HU Xiaohui HAN Quanbin LI Shengbao YU Xianjun Department of Gastroenterology Taihe HospitalHubei University of MedicineShiyan 442000China Laboratory of Inflammation and Molecular Pharmacology School of Basic Medical Sciences&Biomedical Research InstituteHubei Key Laboratory of Wudang Local Chinese Medicine ResearchSchool of PharmacyHubei University of MedicineShiyan 442000China Inflammation-Cancer Transformation and Wudang Chinese Medicine Research Hubei Talent Introduction and Innovation Demonstration BaseHubei University of MedicineShiyan 442000China School of Chinese Medicine Hong Kong Baptist UniversityHong Kong 999077China

Gambogenic acid(GNA),a bioactive compound derived from the resin of Garcinia hanburyi,has demonstrated significant antitumor ***,its mechanisms of action in oral squamous cell carcinoma(OSCC)remain largely *** study aimed to elucidate the apoptotic effects of GNA on OSCC cell lines CAL-27 and *** results indicated that GNA induced apoptosis by upregulating the pro-apoptotic protein *** investigations revealed that GNA treatment led to the generation of reactive oxygen species(ROS),which activated endoplasmic reticulum(ER)stress,culminating in cell *** of ROS production and ER stress pathways significantly mitigated GNA-induced Noxa upregulation and subsequent ***,in vivo studies using a murine xenograft model demonstrated that GNA administration effectively inhibited the growth of CAL-27 ***,these findings underscore GNA’s potential as a therapeutic agent for the treatment of OSCC.

关键词： Oral squamous cell carcinoma Gambogenic acid Apoptosis Noxa ROS/ER stress

Establishing Modular Cell-Free Expression System for the Biosynthesis of Bicyclomycin from a Chemically Synthesized Cyclodipeptide

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Chinese Journal of Chemistry 2024年第4期42卷 384-390页

作者： Yi-Pei Liu Yu-Heng Zhao wen-Qi Zhang Lian Wu Linjuan Huang gong-Li Tang Jun-Bin He Shaanxi Natural Carbohydrate Resource Engineering Research Center College of Food Science and TechnologyNorthwest UniversityXi'anShaanxi710069 China State Key Laboratory of Chemical Biology Shanghai Institute of Organic ChemistryUniversity of Chinese Academy of Sciences(CAS)CASShanghai200032 China School of Chemistry and Materials Science Hangzhou Institute for Advanced StudyUniversity of CAS1 Sub-lane XiangshanHangzhouZhejiang310024 China

Cell-free expression systems have emerged as a versatile and powerful platform for metabolic engineering,biosynthesis and synthetic biology ***,successful examples of the synthesis of complex natural products using this system are still ***,a structurally unique and complex diketopiperazine alkaloid,is a clinically promising antibiotic that selectively inhibits the transcription termination factor ***,we established a modular cell-free expression system with cascade catalysis for the biosynthesis of bicyclomycin from a chemically synthesized *** six cell-free expressed biosynthetic enzymes,including five iron-andα-ketoglutarate-dependent dioxygenases and one cytochrome P450 monooxygenase,were active in converting their substrates to the corresponding *** co-expressed enzymes in the cell-free module were able to complete the related partial *** vitro biosynthesis of bicyclomycin was also achieved by reconstituting the entire biosynthetic pathways(i.e.,six enzymes)using the modular cell-free expression *** study demonstrates that the modular cell-free expression system can be used as a robust and promising platformforthe biosynthesis of complex antibiotics.

关键词： Natural products Bicyclomycin Biosynthesis Iron-andα-ketoglutarate-dependent dioxygenases Cytochrome P450 monooxygenase Cell-freeprotein synthesis Enzyme catalysis

维普期刊数据库评论

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维普期刊数据库

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基于GIS的帽儿山景观生态综合评价

水土保持研究 2010年第4期17卷 139-143页

作者：孔达龚文峰黑龙江大学水利电力学院哈尔滨150086

景观生态综合评价对区域区划有重要的意义,以黑龙江帽儿山为例,以2006年林相分布图、LandsatTM遥感数据、地形图、二类调查数据和其他数据材料为数据源,以GIS为技术手段,选取景观类型破碎度、景观形状指数、土壤侵蚀类型、地形因素(坡度... 详细信息

景观生态综合评价对区域区划有重要的意义,以黑龙江帽儿山为例,以2006年林相分布图、LandsatTM遥感数据、地形图、二类调查数据和其他数据材料为数据源,以GIS为技术手段,选取景观类型破碎度、景观形状指数、土壤侵蚀类型、地形因素(坡度)、土地利用类型、植被盖度指数与气象因子(年降水量)7个指标对研究区域进行景观生态综合评价,在此基础上进行景观生态区划。研究结果表明:(1)全面分析景观生态综合评价指数并进行分级处理和景观区划,分为Ⅰ、Ⅱ、Ⅲ、Ⅳ和Ⅴ级区域,分别代表好、较好、中、较差和差5个级别;(2)不同景观生态综合评价区域面积所占比例的排列顺序为:Ⅱ>Ⅲ>Ⅳ>Ⅰ>Ⅴ,Ⅱ级区域所占的比例最大,在整个研究区域中占据绝对的优势,区域景观生态质量属于较好水平;(3)景观生态质量垂直分异明显,第一、二高程带的景观质量较好,Ⅱ级区域在一、二高程带所占比例较大,在第二高程带达到最大,第三、四和五高程带的景观质量较差。可见基于DEM模型和景观生态综合评价揭示景观生态质量的空间特征。

关键词：景观指标景观生态综合评价景观综合评价模型生态环境质量

基于RS和GIS的嫩江下游土地沙漠化景观格局变化特征分析

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生态与农村环境学报 2009年第3期25卷 99-103页

作者：郭红龚文峰董隽王丹范文义哈尔滨学院地理系黑龙江哈尔滨150086 黑龙江大学水利电力学院黑龙江哈尔滨150086 东北林业大学林学院黑龙江哈尔滨150040

以2004、2008年的MODIS数据为主要数据源,在ArcGIS 9.0、ERDAS IMAGE 9.0等软件支持下,利用FRAGSTATS软件分析土地沙漠化的景观格局变化。结果表明,研究区域沙漠化土地以轻度和中度沙漠化土地为主;各景观要素的边缘密度和斑块密度呈现... 详细信息

以2004、2008年的MODIS数据为主要数据源,在ArcGIS 9.0、ERDAS IMAGE 9.0等软件支持下,利用FRAGSTATS软件分析土地沙漠化的景观格局变化。结果表明,研究区域沙漠化土地以轻度和中度沙漠化土地为主;各景观要素的边缘密度和斑块密度呈现一致性的减少,表明斑块形状趋向简单,景观整体破碎化程度降低,边缘效应影响减小;除水域景观外,其他景观要素的形状指数和分维数都呈现一致性的下降,表明整体景观的形状都向着简单化和稳定性方向发展,各景观要素的散布与并列指数和聚集度指数都呈现一致性的增加,反映出各要素斑块趋向大型化发展,景观整体连接度增强,破碎化程度降低,景观相互作用和空间关系增强。

关键词：景观格局土地沙漠化 MODIS 动态变化

Study on the clogging mechanism of punching screen in sand control by the punching structure parameters

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Petroleum Science 2024年第1期21卷 609-620页

作者： Fu-Cheng Deng Fu-Lin Gui Bai-Tao Fan Lei wen Sheng-Hong Chen Ning gong Yun-Chen Xiao Zhi-Hui Xu Yangtze University Jingzhou434023HubeiChina CNOOC Research Institute Ltd. Beijing100028China CNOOC Ener Tech-Drilling&Production Co. Tianjin300457China Tianjin Branch of CNOOC Ltd. Tianjin300459China

As an independent sand control unit or a common protective shell of a high-quality screen,the punching screen is the outermost sand retaining unit of the sand control pipe which is used in geothermal well or oil and gas ***,most screens only consider the influence of the internal sand retaining medium parameters in the sand control performance design while ignoring the influence of the plugging of the punching screen on the overall sand retaining performance of the *** explore the clogging mechanism of the punching screen,this paper established the clogging mechanism calculation model of a single punching screen sand control unit by using the computational fluid mechanics-discrete element method(CFD-DEM)combined *** to the combined motion of particles and fluids,the influence of the internal flow state on particle motion and accumulation was *** results showed that(1)the clogging process of the punching sand control unit is divided into three stages:initial clogging,aggravation of clogging and stability of *** the initial stage of blockage,coarse particles form a loose bridge structure,and blockage often occurs preferentially at the streamline gathering place below chamfering inside the sand control *** the stage of blockage intensification,the particle mass develops into a relatively complete sand bridge,which develops from both ends of the opening to the center of the *** the stable plugging stage,the sand deposits show a“fan shape”and form a“V-shaped”gully inside the punching slot element.(2)Under a certain reservoir particle-size distribution,The slit length and opening height have a large influence on the permeability and blockage rate,while the slit width size has little influence on the permeability and blockage *** microscopic clogging mechanism and its law of the punching screen prevention unit are proposed in this study,which has some field guidance significance for the design of punching screen

关键词： Punching screen Plugging CFD-DEM Size parameter Sand control

松花江流域哈尔滨城区段土壤侵蚀时空格局及动态变化研究

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水土保持通报 2009年第5期29卷 56-60,84+247页

作者：龚文峰蔡体久范文义黑龙江大学水利水电学院黑龙江哈尔滨150086 东北林业大学林学院黑龙江哈尔滨150040

以松花江流域哈尔滨城区段为研究区域,以ArcGIS为分析平台,对1995—2005年土壤侵蚀时空格局及其动态变化进行了研究。在DEM基础上,分析土壤侵蚀时空变化地貌特征的空间分布。结果表明,水力侵蚀是松花江流域土壤侵蚀的主要形式,以微度侵... 详细信息

以松花江流域哈尔滨城区段为研究区域,以ArcGIS为分析平台,对1995—2005年土壤侵蚀时空格局及其动态变化进行了研究。在DEM基础上,分析土壤侵蚀时空变化地貌特征的空间分布。结果表明,水力侵蚀是松花江流域土壤侵蚀的主要形式,以微度侵蚀为主;在0—400m高程范围内,微度—轻度、轻度—微度、轻度—中度和中度—强度侵蚀相互转换剧烈;土壤侵蚀变化随着坡度的增加而减少且变化主要集中在坡度小于25°的区域上,0°～6°的区域,土壤侵蚀主要集中在0.5°～5°的坡耕地,6°～15°区域,微度—轻度和轻度—微度侵蚀相互交换相对剧烈;土壤侵蚀变化的坡向特征呈现双峰现象,各种强度水力侵蚀的变化主要集中在阴坡(东北)与阳坡(西南)两个坡向,微度侵蚀变化量在平地的面积比重较大。

关键词：土壤侵蚀时空格局地形因子空间分析

Inonotus obliquus(Chaga)against HFD/STZ-induced glucolipid metabolism disorders and abnormal renal functions by regulating NOS-cGMP-PDE5 signaling pathway

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Chinese Journal of Natural Medicines 2024年第7期22卷 619-631页

作者： feng Yating LIU Jing gong Le HAN Zhaodi ZHANG Yan LI Rongshan LIAO Hui School of Pharmacy Shanxi Medical UniversityTaiyuan 030001China Drug Clinical Trial Institution The Fifth Hospital of Shanxi Medical University(Shanxi Provincial People’s Hospital)Taiyuan 030012China Department of Nephrology The Fifth Hospital of Shanxi Medical University(Shanxi Provincial People’s Hospital)Taiyuan 030012China

Our prior investigations have established that Inonotus obliquus(Chaga)possesses hypoglycemic *** hyperglycemia is known to precipitate renal function *** functionality of the kidneys is intricately linked to the levels of cyclic guanosine-3',5'-monophosphate(cGMP),which are influenced by the activities of nitric oxide synthase(NOS)and phosphodiesterase(PDE).Enhanced cGMP levels can be achieved either through the upregulation of NOS activity or the downregulation of PDE *** objective of the current study is to elucidate the effects of Chaga on disorders of glucolipid metabolism and renal abnormalities in rats with type 2 diabetes mellitus(T2DM),while concurrently examining the NOS-cGMP-PDE5 signaling pathway.A model of T2DM was developed in rats using a high-fat diet(HFD)combined with streptozotocin(STZ)administration,followed by treatment with Chaga extracts at doses of 50 and 100 mg·kg^(−1)for eight *** findings revealed that Chaga not only mitigated metabolic dysfunctions,evidenced by improvements in fasting blood glucose,total cholesterol,triglycerides,and insulin resistance,but also ameliorated renal function markers,including serum creatinine,urine creatinine(UCr),blood urea nitrogen,24-h urinary protein,and estimated creatinine ***,enhancements in glomerular volume,GBM thickness,podocyte foot process width(FPW),and the mRNA and protein expressions of podocyte markers,such as nephrin and wilms tumor-1,were *** was found to elevate cGMP levels in both serum and kidney tissues by increasing mRNA and protein expressions of renal endothelial NOS and neural NOS,while simultaneously reducing the expressions of renal inducible NOS and *** summary,Chaga counteracts HFD/STZ-induced glucolipid metabolism and renal function disturbances by modulating the NOS-cGMP-PDE5 signaling *** research supports the potential application of Chaga in the clinical prevention and treatment of T2DM and

关键词： Inonotus obliquus Diabetic nephropathy Glucolipid metabolism disorders Renal functions NOS-cGMP-PDE5 signaling

Tropical cyclone genesis over the western north Pacific in La Ni?a decay summers: Comparison between 2018 and 2021

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Science China Earth Sciences 2024年第10期67卷 3098-3109页

作者： Yunyun LIU Zhensong gong China Meteorological Administration Key Laboratory for Climate Prediction Studies National Climate CentreBeijing 100081China Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters Nanjing University of Information Science&TechnologyNanjing 210044China

As the primary interannual signal of variability in the tropical ocean-atmosphere interaction, the El Ni?o-Southern Oscillation has a considerable impact on tropical cyclone(TC) activity over the western North Pacific(WNP). Both 2018 and2021 were La Ni?a decay years, but TC activity over the WNP during the two summers(June–August) showed notable differences. In 2018, summer TC activity was unusually high with a total of 18 TCs, and the region of TC genesis was mainly in the central and eastern WNP. In contrast, only 9 TCs were generated in summer 2021, and the region of TC genesis was primarily in the western WNP. By comparing the characteristics of the large-scale environmental conditions over the regions of TC genesis, the thermal factors of the tropical oceans, and the activity of the Madden-Julian Oscillation(MJO), this study revealed the possible causes for the marked differences in TC genesis over the WNP during the two summers, which both had a similar background of La Ni?a decay. The Indian Ocean Basin Mode(IOBM) transitioned of a cold anomaly in the winter of 2017/2018and persisted until summer 2018. At the same time, the Pacific Meridional Mode(PMM) maintained a positive phase, leading to eastward and northward displacement of the Western Pacific Subtropical High in summer, and eastward extension of the tropical monsoon trough, which presented conditions conducive to TC genesis over the Northwest Pacific. Moreover, the days when the MJO stagnated in phases 5 and 6 in the summer of 2018 increased by approximately 150% relative to climatological state,providing dynamic conditions favorable for TC formation. In 2021, the IOBM quickly turned to a warm anomaly in March and persisted until summer, whereas the PMM became a negative phase in January and remained so until summer. At the same time,the MJO stagnated in phases 2 and 3 for up to 47 days, with the center of convection located over the western Maritime Continent, producing conditions unconducive to TC ge

关键词： Tropical cyclone La Nina events Indian Ocean Basin Mode Pacific Meridional Mode Madden-Julian Oscillation Western Pacific Subtropical High Monsoon trough