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Comparative Performance of Microscopy and Nested PCR for the detection of Cryptosporidium Species in Patients living with HIV/AIdS in Abidjan (Côte d’Ivoire)

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American Journal of Molecular biology 2023年第1期13卷 18-31页

作者： N’docho Assi Fiacre-Tanguy gonédélé bi sery ernest Tuo Karim bialé Marina danielle datcho-Gouenet Ayaud N’Guessan Tiacoh landry Koffi Attoungbré Clément Koffi david Ako Ako Aristide Bérenger Vanga Bosson Abo Henriette Touré Offianan André department of Parasitology Mycology Institut Pasteur Abidjan C&#244 te d’Ivoire laboratory of Genetics and Species Improvement Félix Houphou&#235 t-Boigny University Abidjan C&#244 te d’Ivoire department of Parasitology Mycology UFR Pharmaceutical and Biological Sciences Félix Houphou&#235 t-Boigny University Abidjan C&#244 te d’Ivoire

Cryptosporidium spp. infection is one of the causes of diarrhea in people living with HIV/AIdS. The objective of this study is to compare the sensitivity of microscopy and molecular biology to determine the prevalence of Cryptosporidium spp. in Patients living With HIV (PlWH). This is a descriptive cross-sectional study conducted in three care centers for people living with HIV/AIdS in Abidjan. It took place from November 2018 to March 2020. Sociodemographic data were obtained via a questionnaire. Stool and blood samples were collected and analyzed for microscopy and Nested PCR detection of Cryptosporidium spp. Blood samples were analyzed for Cd4+ count. A total of 363 stool samples were collected from the three sites. Individuals aged 40 - 50 years (36.52%) were most likely to participate in the study. HIV Type 1 accounted for 86.22% of the study population. The samples collected consisted of 47.65% diarrheal stool. Microscopic examination of the stool yielded a prevalence of 3.86% for Cryptosporidium spp. while the prevalence was 3.96% with molecular identification. No statistically significant difference was observed between these two prevalences (χ2 = 0.26;p = 0.609). Cd4+ count was the factor associated with Cryptosporidium spp. infection for both microscopy (OR = 0.887, p = 0.001) and PCR (OR = 0.896, p = 0.001). This study demonstrated that Nested PCR improves the detection of Cryptosporidium spp. in patient diagnosis.

关键词： Cryptosporidium PCR Microscopy PlHIV Côte d’Ivoire

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