检索结果-南通市图书馆

柚皮苷改善束缚性应激引起的小鼠生化指标及行为变化的一氧化氮调节机制(英文)

在线全文

学校读者我要写书评

暂无评论

中西医结合学报 2011年第11期9卷 1254-1263页

作者： gollapalle l.viswanatha Hanumanthappa Shylaja K.Sadashiva Sandeep Rao Yathiraj Ashwini V.Ramaiah Santhosh Kumar C.Gangadharaiah Mohan Venkate Gowda Sunil M.Venkateshappa Sarvesh Kumar Subbanna Rajesh Department of Pharmacology People's Education Society College of PharmacyBangalore 560050India Department of Pharmacognosy People's Education Society College of PharmacyBangalore 560050India Biomedical Research Centre Sheffield Hallam UniversitySheffield S11 WUK Department of Biochemistry Kasturba Medical CollegeMangalore 575001India Department of Pharmacology Al-Ameen College of PharmacyBangalore 560027India Department of Pharmacognosy Government College of PharmacyBangalore 560027India Department of Biotechnology Oxford College of SciencesBangalore 560078India Department of Microbiology Indian Academy Centre for Research and Postgraduate StudiesBangalore 560040India

目的:研究柚皮苷对束缚性应激引起的小鼠生化指标改变、行为变化及线粒体功能紊乱的影响。方法:将小鼠按照体质量随机分组,分别给予不同药物治疗14 d。在第15天让所有小鼠接受束缚性应激刺激6 h,然后在接受各种不同的行为测试后处死。... 详细信息

目的:研究柚皮苷对束缚性应激引起的小鼠生化指标改变、行为变化及线粒体功能紊乱的影响。方法:将小鼠按照体质量随机分组,分别给予不同药物治疗14 d。在第15天让所有小鼠接受束缚性应激刺激6 h,然后在接受各种不同的行为测试后处死。取小鼠的大脑匀浆进行各种生化指标测量和线粒体功能分析。结果:6 h的急性束缚性应激刺激能显著改变小鼠的行为(焦虑和记忆),引起生化指标变化和线粒体功能紊乱。用柚皮苷预处理(50或100 mg/kg口服)14 d则能显著减轻急性束缚性应激刺激引起的小鼠行为和生化指标变化以及线粒体功能紊乱(P<0.05);而使用一氧化氮的前体左旋精氨酸预处理(50 mg/kg腹腔注射)可以拮抗柚皮苷的保护效应(P<0.05)。此外,采用左旋精氨酸甲酯预处理(5 mg/kg腹腔注射)则导致柚皮苷的保护效应增强。结论:柚皮苷对束缚性应激刺激引起的小鼠行为和生化指标变化以及线粒体功能紊乱的保护作用与氮能神经通路有关。

关键词：柚皮苷约束身体的应激一氧化氮脂质过氧化作用

Antioxidant and antimutagenic activities of bark extract of Terminalia arjuna

在线全文

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Medicine 2010年第12期3卷 965-970页

作者： gollapalle lakshminarayana shastry viswanatha Santosh Kumar Vaidya Ramesh C Nandakumar Krishnadas Srinath Rangappa Department of Pharmacology PES College of PharmacyBangalore-560050 India Department of Pharmacology St.John's College of PharmacyBangalore-560040 India Department of Pharmacology Manipal College of Pharmaceutical SciencesManipal-576104 India

Objective:The alcoholic extract of stem bark of Terminalia arjuna（AlTA） was screened for antioxidant and antimutagenic（anticlastogenic） ***:Antioxidant property was determined by l,l,Diphynyl,2-Picryl hydrazyl（DPPH） assay,super oxide radical scavenging activity,lipid peroxidation assay and total polyphenolic content was determined by Folin-Ciocalteau’s *** activity was evaluated using micronucleus test in mice. Results:The AlTA has shown potent antioxidant activity with EC50 of 2.491±0.160,50.110±0.150 & 71.000±0.250 in DPPH assay,superoxide radical scavenging activity and lipid peroxidation assay, which is comparable with ascorbic acid with EC50 of 2.471±0.140,40.500±0.390 and 63.000±0.360 *** micronucleus test,AlTA（100 & 200 mg/kg,p.o.） showed significant reduction in percentage of micronucleus in both polychromatic erythrocytes（PCE） and normochromatie erythrocytes（NCE） and also shown significant reduction in P/N ***:These results suggested that AlTA possess significant antioxidant and antimutagenic activity.

关键词： Terminalia arjuna Antioxidant activity Antimutagenic activity Micronuclease test

Methanolic extract of Momordica cymbalaria enhances glucose uptake in l6 myotubes in vitro by up-regulating PPAR-γ and GlUT-4

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Natural Medicines 2014年第12期12卷 895-900页

作者： Puttanarasaiah Mahesh Kumar Marikunte V Venkataranganna Kirangadur Manjunath gollapalle l.viswanatha Godavarthi Ashok Padmashree Institute of Information Sciences Connexios life Sciences Pvt ltd. J.P.Nagar Bangalore 560078 India Department of Microbiology and Biotechnology Bangalore University PES College of Pharmacy Radiant Research Services Pvt ltd.

The present study was undertaken to evaluate the influence of the methanolic fruit extract of Momordica cymbalaria （MFMC） on PPARγ, （Peroxisome Proliferator Activated Receptor gamma） and GlUT-4 （Glucose transporter-4） with respect to glucose transport. Various concentrations of MFMC ranging from 62.5 to 500 μg·ml^-1 were evaluated for glucose uptake activity in vitro using l6 myotubes, rosiglitazone was used as a reference standard. The MFMC showed significant and dose-dependent increase in glucose uptake at the tested concentrations, further, the glucose uptake activity of MFMC （500 μg·ml^-1） was comparable with rosigilitazone. Furthermore, MFMC has shown up-regulation of GlUT-4 and PPARy gene expressions in l6 myotubes. In addition, the MFMC when incubated along with cycloheximide （CHX）, which is a protein synthesis inhibitor, has shown complete blockade of glucose uptake. This indicates that new protein synthesis is required for increased GlUT-4 translocation. In conclusion, these findings suggest that MFMC is enhancing the glucose uptake significantly and dose dependently through the enhanced expression of PPARμ and GlUT-4 in vitro.

关键词： Momordica cymbalaria Diabetes Glucose transport PPARy l6 myotubes Antidiabetic activity

液相色谱-串联质谱法测定辣木及萝卜叶的提取物中所含的槲皮素、芦丁及山柰酚(英文)

在线全文

学校读者我要写书评

暂无评论

中西医结合学报 2011年第9期9卷 1022-1030页

作者： Venkatapura C. Devaraj Burdipad G. Krishna gollapalle l. viswanatha Department of Pharmacology Bioneeds Laboratory Animals and Preclinical Services Department of Pharmacognosy R.R College of Pharmacy Department of Pharmacology People's Education Society College of Pharmacy

目的：通过一种快速、敏感的液相色谱-串联质谱法测定辣木（Moringa oleifera lam.）及萝卜（Raphinus sativus linn.）叶的提取物中所含的槲皮素、芦丁及山柰酚。方法：使用冷浸法（90%乙醇）对辣木及萝卜叶进行提取。提取物与0.2%甲... 详细信息

目的：通过一种快速、敏感的液相色谱-串联质谱法测定辣木（Moringa oleifera lam.）及萝卜（Raphinus sativus linn.）叶的提取物中所含的槲皮素、芦丁及山柰酚。方法：使用冷浸法（90%乙醇）对辣木及萝卜叶进行提取。提取物与0.2%甲酸和氰化甲烷混合后以0.4ml/min的速度通过Phenomenex Gemini C18色谱层析柱,通过时间为5.01min。结果：串联质谱法测定的离子转换分别为槲皮素303.03~153.1,芦丁611.1~303.1,山柰酚287.1~153.2,内参180.1~110.1。对槲皮素、芦丁及山柰酚定量测量的最低浓度为5ng/ml,线性分布为5~2000ng/ml。槲皮素、芦丁及山柰酚的线性回归系数分别为0.9946、0.9951及0.9969。结论：本研究的结果证明了液相色谱-串联质谱法具有快速、灵敏的特点,对于同时测定辣木及萝卜叶的提取物中所含的槲皮素、芦丁及山柰酚有较好的特异性。

关键词：辣木萝卜植物提取物槲皮素芦丁山柰酚色谱法,液相串联质谱法

印度草药方NR/CAl/06的甲醇提取物对卵巢切除大鼠的抗骨质疏松作用(英文)

在线全文

学校读者我要写书评

暂无评论

中西医结合学报 2011年第10期9卷 1125-1132页

作者： Praveen Srikanta Shivaprasad H.Nagarajappa gollapalle l.viswanatha. Mukund Handral Rajesh Subbanna Rangappa Srinath Ganesh Hiremath Department of Pharmacology People's Education Society(PES)College of Pharmacy Natural Remedies Pvt.ltd Veerasandra Industrial Area

目的:证实印度草药方NR/CAl/06的甲醇提取物对卵巢切除大鼠的抗骨质疏松作用。方法:雌性Sprague-Drawley大鼠在无菌状态下行双侧卵巢切除术后被分为5组(n=10)。检测NR/CAl/06的甲醇提取物(200和400mg/kg口服)的抗骨质疏松作用并以雷洛昔... 详细信息

目的:证实印度草药方NR/CAl/06的甲醇提取物对卵巢切除大鼠的抗骨质疏松作用。方法:雌性Sprague-Drawley大鼠在无菌状态下行双侧卵巢切除术后被分为5组(n=10)。检测NR/CAl/06的甲醇提取物(200和400mg/kg口服)的抗骨质疏松作用并以雷洛昔芬(5.4mg/kg口服)作为标准对照药,治疗时间为90d。实验前后测量各组大鼠的体质量、卵巢质量、骨质量、骨矿物质含量、骨强度,血清及尿液中的钙、磷含量及血清碱性磷酸酶含量。电镜下观察大鼠股骨外径、长度及厚度。结果:双侧卵巢切除术后,大鼠骨质量、骨矿物质含量及骨强度均有下降;电镜观察到卵巢切除大鼠股骨骨质疏松、多孔、破碎,且骨长度及厚度均有所下降。与假手术组相比,卵巢切除大鼠的血清钙、磷及碱性磷酸酶含量均有所升高,体质量及脂肪含量升高,子宫质量降低。NR/CAl/06的甲醇提取物(200和400mg/kg口服)治疗90d能够剂量依赖性地改变大鼠因卵巢切除所引起的上述各项指标变化,并将骨质量、骨矿物质含量、骨强度,血清钙、磷及碱性磷酸酶含量,体质量及脂肪含量恢复至正常水平。此外,经NR/CAl/06的甲醇提取物(200和400mg/kg口服)治疗的大鼠与卵巢切除模型组大鼠相比,电镜下股骨骨质孔形成减少,骨密度增加。结论:本实验的结果证实了NR/CAl/06的甲醇提取物具有很好的抗骨质疏松作用,可用于骨质疏松症的治疗。

关键词：骨质疏松卵巢切除术植物提取物医学,印度传统显微镜检查,电子,扫描大鼠