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Impact of Fractures and Diagenesis on Reservoir Potential of Inner Ramp Paleocene Carbonates Exposed in Western Part of the Lesser Himalayas of Pakistan

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Journal of Earth Science 2023年第2期34卷 536-555页

作者： Waleed Tariq gohar rehman Syed Ahsan Hussain Gardezi Nawaz Ikram National Center of Excellence in Geology University of PeshawarPeshawarKhyber Pakhtunkhwa 25120Pakistan Department of Geology University of PeshawarPeshawarKhyber Pakhtunkhwa 25120Pakistan Geological Survey of Pakistan Azad Jammu&Kashmir DirectorateMuzaffarabad 13100Pakistan Institute of Geology University of Azad Jammu&KashmirMuzaffarabad 13100Pakistan

The outcrop investigations provide a better comprehension to interrelate facies-diagenesis and fracture networks for the evaluation of reservoir potential of the carbonate rocks.In this paper,we targeted Kahi-Section(Nizampur Basin)and Peeran Tangai-Section(Kalachitta Range)to analyze structural-kinematics,Discrete Fracture Network Modelling,microfacies identification and diagenesis to interpret their impact on reservoir potential of Lockhart Limestone(Paleocene).The structural grain within the study area mostly represents the typical east-west trending tight to overturned folds and north-dipping thrust faults that mimic the north-south Indo-Eurasian collision.However,a second phase of deformation related to east-west compressions also identified which rotated the axes of preexisting structures.Fracture analysis revealed that extensional fractures are oriented at high angle to bedding and are differentiated into three orthogonal sets trending northeast-southwest,northwest-southeast and east-west,whereas,the shear fractures formed two conjugate sets trending northeastsouthwest.The Lockhart Limestone was deposited in the inner ramp setting and microfacies types are packstone,wackestone and wacke-packstone with seven sub-microfacies types.It has been identified that the Lockhart Limestone has the heterogeneous distribution of diagenetic and tectonic features throughout its extent.The observed diagenetic sequence is micritization,calcite cementation,dissolution,neomorphism,pyritization and compaction.The results highlight that open and partially filled fractures may provide an interconnected network to promote fluid mobility,leading to higher values of fracture permeability.The porosity values of the pore matrix were greater than fracture,resulting a significant impact on reservoir storage capacity.In contrast,a negative impact on reservoir potential has been shown by stylolites,veins and dissolution seams.However,based on the overall studies,the Lockhart Limestone revealed the prospect of a good reservoir unit in th

关键词： lockhart limestone discrete fracture network modelling facies-diagenesis reservoir potential Nizampur Basin petroleum geology

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Rice-wheat comparative genomics:Gains and gaps

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The Crop Journal 2024年第3期12卷 656-669页

作者： Akila Wijerathna-Yapa Ruchi Bishnoi Buddhini Ranawaka Manu Maya Magar Hafeez Ur rehman Swati G.Bharad Michal T.Lorenc Vinita Ramtekey Sasha gohar Charu Lata Md.Harun-Or-Rashid Maryam Razzaq Muhammad Sajjad Bhoja *** School of Agriculture and Food Sustainability The University of QueenslandSt LuciaQLD 4072Australia ARC Centre of Excellence for Plant Success in Nature and Agriculture The University of QueenslandSt LuciaQLD 4072Australia Department of Genetics and Plant Breeding College of AgricultureUmmedganj-KotaAgriculture UniversityKotaRajasthan 324001India Centre for Agriculture and the Bioeconomy Queensland University of Technology(QUT)2 George StreetBrisbaneQLD 4000Australia UWA School of Agriculture and Environment The University of Western Australia35 Stirling HighwayCrawleyWA 6009Australia Nepal Agriculture Research Council KhumaltarLalitpurNepal Department of Agronomy University of AgricultureFaisalabad 38040Pakistan Department of Agricultural Botany Dr Panjabrao Deshmukh Krishi VidyapeethAkola 444104MaharashtraIndia ICAR-Indian Institute of Seed Science MauUttar Pradesh 275103India School of Natural Sciences Macquarie UniversityMacquarie ParkNSW 2109Australia ICAR-Indian Institute of Wheat and Barley Research Flowrdale 54642ShimlaIndia International Maize and Wheat Improvement Center(CIMMYT)Bangladesh Office Gulshan-2Dhaka 1212Bangladesh Center for Seed Science University of AgricultureFaisalabad 38000Pakistan Department of Biosciences COMSATS University IslamabadPark RoadTarlai KalanIslamabad 45550Pakistan CGIAR’s Excellence in Breeding(EiB)Platform/Accelerated Breeding Initiative International Maize and Wheat Improvement Center(CIMMYT)El Batán 56237Mexico

Rice and wheat provide nearly 40%of human calorie and protein requirements.They share a common ancestor and belong to the Poaceae(grass)family.Characterizing their genetic homology is crucial for developing new cultivars with enhanced traits.Several wheat genes and gene families have been characterized based on their rice orthologs.Rice–wheat orthology can identify genetic regions that regulate similar traits in both crops.Rice–wheat comparative genomics can identify candidate wheat genes in a genomic region identified by association or QTL mapping,deduce their putative functions and biochemical pathways,and develop molecular markers for marker-assisted breeding.A knowledge of gene homology facilitates the transfer between crops of genes or genomic regions associated with desirable traits by genetic engineering,gene editing,or wide crossing.

关键词： Comparative genomics Orthologs Genes Synteny Genetic engineering Molecular breeding

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Prediction of promiscuous T-cell epitopes in the Zika virus polyprotein:An in silico approach

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Asian Pacific Journal of Tropical Medicine 2016年第9期9卷 822-828页

作者： Hamza Dar Tahreem Zaheer Muhammad Talha rehman Amjad Ali Aneela Javed gohar Ayub Khan Mustafeez Mujtaba Babar Yasir Waheed Atta-ur-Rahman School of Applied Biosciences(ASAB) National University of Sciences and Technology Shifa College of Pharmaceutical Sciences Shifa Tameer-e-Millat University Foundation University Medical College Foundation University Islamabad

Objective:To predict immunogenic promiscuous T-cell epitopes from the polyprotein of the Zika virus using a range of bioinformatics tools.To date,no epitope data are available for the Zika virus in the IEDB database.Methods:We retrieved nearly 54 full length polyprotein sequences of the Zika virus from the NCBI database belonging to different outbreaks.A consensus sequence was then used to predict the promiscuous T cell epitopes that bind MHC 1 and MHC II alleles using Propred1 and Propred immunoinformatic algorithms respectively.The antigencity predicted score was also calculated for each predicted epitope using the Vaxi Jen 2.0 tool.Results:By using Pro Pred1,23 antigenic epitopes for HLA class I and 48 antigenic epitopes for HLA class II were predicted from the consensus polyprotein sequence of Zika virus.The greatest number of MHC class I binding epitopes were projected within the NS5(21%),followed by Envelope(17%).For MHC class II,greatest number of predicted epitopes were in NS5(19%) followed by the Envelope,NS1 and NS2(17% each).A variety of epitopes with good binding affinity,promiscuity and antigenicity were predicted for both the HLA classes.Conclusion:The predicted conserved promiscuous T-cell epitopes examined in this study were reported for the first time and will contribute to the imminent design of Zika virus vaccine candidates,which will be able to induce a broad range of immune responses in a heterogeneous HLA population.However,our results can be verified and employed in future efficacious vaccine formulations only after successful experimental studies.

关键词： Zika Virus B-Cell Epitopes T-Cell Epitopes Vaccine Antigenicity

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