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Engineering hyaluronic acid-based nanoassemblies for monoclonal antibody delivery-design,characterization,and biological insights

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Nano Research 2024年第10期17卷 9111-9125页

作者： Ana M.López-Estévez Y.Zhang María Medel Iker Arriaga Lucía sanjurjo Cristian huck-Iriart Nicola G.A.Abrescia María J.Vicent Defang Ouyang Dolores Torres María José Alonso Center for Research in Molecular Medicine and Chronic Diseases(CiMUs) University of Santiago de CompostelaSantiago de Compostela 15782Spain Department of Pharmacology Pharmacy and Pharmaceutical TechnologySchool of PharmacyUniversity of Santiago de CompostelaSantiago de Compostela 15782Spain Health Research Institute of santiago de Compostela(IDIs) Santiago de Compostela 15782Spain state Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical Sciences(ICMS)University of MacaoMacao 999078China Polymer Therapeutics Laboratory and CIBERONC Prince Felipe Research Centre Valencia E-46012Spain structure and Cell Biology of Viruses Lab CIC bioGUNEBasque Research and Technology Alliance(BRTA)Derio 48160Spain ALBA synchrotron Light source Experiments DivisionCerdanyola del VallèsBarcelona 08290Spain IKERBAsQUE Basque Foundation for ScienceBilbao 48009Spain

The current spotlight of cancer therapeutics is shifting towards personalized medicine with the widespread use of monoclonal antibodies(mAbs).Despite their increasing potential,mAbs have an intrinsic limitation related to their inability to cross cell membranes and reach intracellular *** offers promising solutions to overcome this limitation,however,formulation challenges *** challenges are the limited loading capacity(often insufficient to achieve clinical dosing),the complex formulation methods,and the insufficient characterization of mAb-loaded ***,we present a new nanocarrier consisting of hyaluronic acid-based nanoassemblies(HANAs)specifically designed to entrap mAbs with a high efficiency and an outstanding loading capacity(50%,w/w).HANAs composed by an mAb,modified HA and phosphatidylcholine(PC)resulted in sizes of~100 nm and neutral surface *** modeling identified the principal factors governing the high affinity of mAbs with the amphiphilic HA and *** composition and structural configuration were analyzed using the orthogonal techniques cryogenic transmission electron microscopy(cryo-TEM),asymmetrical flow field-flow fractionation(AF4),and small-angle X-ray scattering(sAXs).These techniques provided evidence of the formation of core-shell nanostructures comprising an aqueous core surrounded by a bilayer consisting of phospholipids and amphiphilic *** vitro experiments in cancer cell lines and macrophages confirmed HANAs’low toxicity and ability to transport mAbs to the intracellular *** reproducibility of this assembling process at industrial-scale batch sizes and the long-term stability was *** conclusion,these results underscore the suitability of HANAs technology to load and deliver biologicals,which holds promise for future clinical translation.

关键词： monoclonal antibody nanoparticles hyaluronic acid characterization particle size computational modeling

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Enabling materials informatics for ^(29)si solid-state NMR of crystalline materials

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npj Computational Materials 2020年第1期6卷 1219-1225页

作者： He sun shyam Dwaraknath Handong Ling Xiaohui Qu Patrick huck Kristin A.Persson sophia E.Hayes Department of Chemistry Washington UniversitySt.LouisMO 63130USA Lawrence Berkeley National Laboratory BerkeleyCA 94720USA Department of Materials science and Engineering UC BerkeleyBerkeleyCA 94720USA

Nuclear magnetic resonance(NMR)spectroscopy is a powerful tool for obtaining precise information about the local bonding of materials,but difficult to interpret without a well-vetted dataset of reference *** ability to predict NMR parameters and connect them to three-dimensional local environments is critical for understanding more complex,long-range interactions.

关键词： spectra NMR crystalline

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F8TBT:Molecular Weight Influence on Mobility and solar Cells Performance

F8TBT:Molecular Weight Influence on Mobility and Solar Cells...

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全国第八届有机固体电子过程暨华人有机光电功能材料学术讨论会

作者： Wilhelm.T.s.huck Melville Laboratory for Polymer synthesis Department of ChemistryUniversity of Cambridge

This contribution presents molecular weight(Mn) controlling strategy to optimize F8TBT as electron donor in solar *** presented examples include four F8TBTs with Mn from 5K to 167K *** results show that this amorphous...

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High-throughput predictions of metal-organic framework electronic properties:theoretical challenges,graph neural networks,and data exploration

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npj Computational Materials 2022年第1期8卷 1053-1062页

作者： Andrew s.Rosen Victor Fung Patrick huck Cody T.O’Donnell Matthew K.Horton Donald G.Truhlar Kristin A.Persson Justin M.Notestein Randall Q.snurr Department of Materials science and Engineering University of CaliforniaBerkeleyCA94720USA Miller Institute for Basic Research in science University of CaliforniaBerkeleyBerkeleyCA94720USA Materials science Division Lawrence Berkeley National LaboratoryBerkeleyCA94720USA Department of Chemical and Biological Engineering Northwestern UniversityEvanstonIL60208USA Center for Nanophase Materials sciences Oak Ridge National LaboratoryOak RidgeTN37831USA Energy Technologies Area Lawrence Berkeley National LaboratoryBerkeleyCA94720USA Department of Chemistry Chemical Theory Centerand Minnesota Supercomputing InstituteUniversity of MinnesotaMinneapolisMN55455USA Molecular Foundry Lawrence Berkeley National LaboratoryBerkeleyCA94720USA

With the goal of accelerating the design and discovery of metal–organic frameworks(MOFs)for electronic,optoelectronic,and energy storage applications,we present a dataset of predicted electronic structure properties for thousands of MOFs carried out using multiple density functional *** to more accurate hybrid functionals,we find that the widely used PBE generalized gradient approximation(GGA)functional severely underpredicts MOF band gaps in a largely systematic manner for semi-conductors and insulators without magnetic ***,an even larger and less predictable disparity in the band gap prediction is present for MOFs with open-shell 3d transition metal *** regards to partial atomic charges,we find that different density functional approximations predict similar charges overall,although hybrid functionals tend to shift electron density away from the metal centers and onto the ligand environments compared to the GGA point of *** more significant differences in partial atomic charges are observed when comparing different charge partitioning *** conclude by using the dataset of computed MOF properties to train machine-learning models that can rapidly predict MOF band gaps for all four density functional approximations considered in this work,paving the way for future high-throughput screening *** encourage exploration and reuse of the theoretical calculations presented in this work,the curated data is made publicly available via an interactive and user-friendly web application on the Materials Project.

关键词： charges electronic centers

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Temperature-Programmed Reduction and Dispersive X-Ray Absorption spectroscopy studies of CeO<sub>2</sub>-Based Nanopowders for Intermediate-Temperature solid-Oxide Fuel Cell Anodes

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Materials sciences and Applications 2019年第10期10卷 631-642页

作者： Marina s. Bellora Joaquí n sacanell Cristiá n huck-Iriart Analí a L. soldati susana A. Larrondo Diego G. Lamas CONICET/Laboratorio de Cristalografía Aplicada Escuela de Ciencia y Tecnología Universidad Nacional de General San Martín San Martín Argentina Departamento de Física de la Materia Condensada Gerencia de Investigación y Aplicaciones Centro Atómico Constituyentes Comisión Nacional de Energía Atómica San Martín Argentina Instituto de Nanociencia y Nanotecnología (INN) CNEA-CONICET San Martín and San Carlos de Bariloche Argentina UNIDEF-CONICET-MINDEF Departamento de Investigaciones en Sólidos CITEDEF Villa Martelli Argentina Instituto de Investigación e Ingeniería Ambiental Universidad Nacional de General San Martín San Martín Argentina

In this work, we study the influence of the average crystallite size and dopant oxide on the reducibility of CeO2-based nanomaterials. samples were prepared from commercial Gd2O3-, sm2O3- and Y2O3-doped CeO2 powders by calcination at different temperatures ranging between 400°C and 900°C and characterized by X-ray powder diffraction, transmission electron microscopy and BET specific surface area. The reducibility of the samples was analyzed by temperature-programmed reduction and in situ dispersive X-ray absorption spectroscopy techniques. Our results clearly demonstrate that samples treated at lower temperatures, of smallest average crystallite size and highest specific surface areas, exhibit the best performance, while Gd2O3-doped ceria materials display higher reducibility than sm2O3- and Y2O3-doped CeO2.

关键词： Crystallite size CeO2-Based Nanomaterials sm2O3

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Imaging for deep brain stimulation:The zona incerta at 7 Tesla

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World Journal of Radiology 2013年第1期5卷 5-16页

作者： Hans U Kerl Lars Gerigk Marc A Brockmann sonia huck Mansour AlZghloul Christoph Groden Thomas Hauser Armin M Nagel Ingo s Nlte Department of Neuroradiology University Medical Center MannheimUniversity of Heidelberg68167 MannheimGermany Division of Radiology German Cancer Research Center69120 HeidelbergGermany Department of Medical Physics and Radiology German Cancer Research Center69120 HeidelbergGermany

AIM:To evaluate different promising magnetic resonance imaging(MRI) methods at 7.0 Tesla(T) for the pre-stereotactic visualization of the zona incerta(ZI).METHODs:Two neuroradiologists qualitatively and quantitatively examined T2-turbo spin-echo(T2-TsE),T1-weighted gradient-echo,as well as FLAsH2D-T2star and susceptibility-weighted imaging(sWI) for the visualization of the ZI at 7.0 T *** and image quality for the ZI were independently examined using a 6-scale grading ***-rater reliability using Cohen's kappa coefficient(κ) were ***-tonoise ratios(CNR),and signal-to-noise ratios(sNR) for the ZI were calculated for all *** in delineation,sNR,and CNR between the sequences were statistically assessed using a paired *** the anatomic validation the coronal FLAsH2D-T2star images were co-registered with a stereotactic atlas(schaltenbrand-Wahren).REsULTs:The rostral part of the ZI(rZI) could easily be identified and was best and reliably visualized in the coronal FLAsH2D-T2star *** caudal part was not definable in any of the *** major artifacts in the rZI were observed in any of the ***2D-T2star and sWI imaging offered significant higher CNR values for the rZI compared to T2-TsE images(P > 0.05).The co-registration of the coronal FLAsH2D-T2star images with the stereotactic atlas schema(schaltenbrand-Wahren) confirmed the correct localization of the ZI in all ***:FLAsH2D-T2star imaging(particularly coronal view) provides the reliable and currently optimal visualization of the rZI at 7.0 *** results can facilitate a better and more precise targeting of the caudal part of the ZI than ever before.

关键词： Deep brain stimulation Essential tremor Magnetic resonance imaging Parkinson’s disease Zona incerta 7 Tesla

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Plant fertility defects induced by the enhanced expression of microRNA167

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Cell Research 2006年第5期16卷 457-465页

作者： Peng Ru~1 Lin Xu~1 Hong Ma~(2,3) Hai Huang~(1,2) ~1National Laboratory of Plant Molecular Genetics,shanghai Institute of Plant Physiology and Ecology,shanghai Institute for Biological sciences,300 Fenglin Road,shanghai 200032,China ~2sIBs-sJTU-PsU Joint Center for Life sciences,shanghai Institute of Plant Physiology and Ecologgy,shanghai Institute for Biological sciences,300 Fenglin Road,shanghai 200032,China ~3Department of Biology and the huck Institute for the Life sciences,The Pennsylvania state University,University Park,PA 16802,UsA National Laboratory of Plant Molecular Genetics Shanghai Institute of Plant Physiology and Ecology Shanghai Institute for Biological Sciences Shanghai China sIBs-sJTU-PsU Joint Center for Life sciences Shanghai Institute of Plant Physiology and Ecology Shanghai Institute for Biological Sciences Shanghai China Department of Biology and the huck Institute for the Life sciences The Pennsylvania State University University Park USA

The plant hormone auxin plays a critical role in regulating plant growth and *** advances havebeen made in the understanding of auxin response pathways,primarily by the characterization of auxin response mu-tants in *** addition,microRNAs (miRNAs) have been shown to be critical regulators of genes importantfor normal plant development and ***,little is known about possible interactions between miRNAsand hormonal signaling during norlnal *** we show that an Arabidopsis microRNA,miR167,whichhas a complementary sequence to a portion of the A UXIN REsPONsE FACTOR6 (ARF6) and ARF8 mRNAs,can causetranscript degradation for ARFs,but not for *** report phenotypic characterizations of35s::MIR167b transgeniclines,and show that severe 35s::MIR167b transgenic lines had phenotypes similar to those of an arf6 arf8 double *** transgenic phenotypes suggest that miR167 may repress ARF6 at the level of *** demonstrate that thetransgenic plants are defective in all four whorls of floral *** the transgenic flowers,filaments were abnormallyshort,anthers could not properly release pollen,and pollen grains did not *** results provide an importantlink between the miRNA-mediated regulatory pathway of gene expression and the auxin signaling network promotingplant reproductive development.

关键词： Arabidopsis auxin response factor microRNA167 floral organ pollen germination

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Proteomic identification of potential target proteins regulated by an AsK1-mediated proteolysis pathway

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Cell Research 2006年第5期16卷 489-498页

作者： Kaiming Cao state Key Laboratory of Genetic Engineering Department of Biochemistry and Molecular Biology school of Life sciences Fudan University shanghai 200433 China

The AsK1 (ARABIDOPsIs sKP1-LIKE) protein is a critical component of the sCF (skpl-Cullin-F box protein)ubiquitin ligase complexes that recruit target proteins for degradation by the 26s *** investigate proteinsthat are affected by the AsK1-mediated proteolysis pathway in Arabidopsis flowers,we compared the proteomes of theArabidopsis wild type and ask1 mutant flower buds using two-dimensional electrophoresis (2-DE).Ten protein spots withhigher or lower abundance in the ask1 mutant flowers compared to wild type flowers were excised and subjected to furthermass spectrometry (Ms) *** results showed that they were proteins involved in photomorphogenesis,circadianoscillation,post-translation process,stress-responses and cell expansion or elongation,suggesting that those processeswere affected in the ask1 *** transcript levels of these genes were also compared based on the Affymetrix genechip microarray *** significant difference was observed for most of the genes,suggesting that the proteins withelevated levels of accumulation in the ask1 mutant could be candidate targets regulated by an AsKl-mediated *** results help to elucidate the pleiotropic functions of AsK1 in Arabidopsis developmental processes andalso demonstrate the importance and necessity of studying protein levels with respect to gene functions.

关键词： AsK1 two-dimensional polyacrylamide gel electrophoresis mass spectrometry proteolysis

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Rice Mitogen-activated Protein Kinase Gene Family and Its Role in Biotic and Abiotic stress Response

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Journal of Integrative Plant Biology 2007年第6期49卷 751-759页

作者： Jai s. Rohila Yinong Yang Department of Plant Pathology and the huck Institutes of the Life sciences Pennsylvania State University University Park PA 16802 USA

The mitogen-activated protein kinase （MAPK） cascade is an important signaling module that transduces extracellular stimuli into intracellular responses in eukaryotic organisms. An increasing body of evidence has shown that the MAPK-mediated cellular signaling is crucial to plant growth and development, as well as biotic and abiotic stress responses. To date, a total of 17 MAPK genes have been Identified from the rice genome. Expression profiling, biochemical characterization and/or functional analysis were carried out with many members of the rice MAPK gene family, especially those associated with biotic and abiotic stress responses. In this review, the phylogenetic relationship and classification of rice MAPK genes are discussed to facilitate a simple nomenclature and standard annotation of the rice MAPK gene family. Functional data relating to biotic and abiotic stress responses are reviewed for each MAPK group and show that despite overlapping in functionality, there is a certain level of functional specificity among different rice MAP kinases. The future challenges are to functionally characterize each MAPK, to identify their downstream substrates and upstream kinases, and to genetically manipulate the MAPK signaling pathway in rice crops for the Improvement of agronomically important traits.

关键词： abiotic stress tolerance disease resistance mitogen-activated protein kinase Oryza sativa signal transduction.

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Temperature-Programmed Reduction and Dispersive X-Ray Absorption spectroscopy studies of CeO2-Based Nanopowders for Intermediate-Temperature solid-Oxide Fuel Cell Anodes

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Materials sciences and Applications（材料科学与应用期刊） 2019年第10期

作者： Marina s. Bellora Joaquín sacanell Cristián huck-Iriart Analía L. soldati susana A. Larrondo Diego G. Lamas

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