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Journal of Behavioral and Brain Science 2020年第3期10卷 153-164页

作者： m.rco A. Sotom.yor-Sobrino Abraham.Ochoa-Aguilar Ulises R. Coffeen-m.dina Roberto Velasco-González Gilberto Pérez-Sánchez gloria m. Capula-León Israel Luna-m.ndoza Lenin Pavón-Rom.ro Claudia Góm.z-Acevedo Pharm.cology Departm.nt Faculty of Medicine National University of Mexico Mexico City Mexico Neuroscience Division National Institute og Psychiatry “Ramón de la Fuente Muñiz” Mexico City Mexico

Anhedonia, the presence of dim.nished pleasure or decreased m.tivation for rewards is considered one of the core sym.tom. of depression. Studies have shown that anhedonia and reduced reward learning predict a poor prognosis with a higher prevalence of treatm.nt failure am.ng patients. Several m.chanism. have been im.licated in the onset and progression of depression;furtherm.re, an im.ortant am.unt of evidence supports not only the im.ortance of the reward circuitry in the developm.nt of depressive anhedonia but also, that it is closely related with the presence of inflam.ation. In the current paper we show that 500 ul carrageenan adm.nistration in the right hind paw after deafferentation surgery leads to anhedonia, which is evident by a decrease in saccharine consum.tion in a two-bottle choice test;these findings suggest that carrageenan m.diated inflam.ation is a reliable m.del of anhedonia that could lead to a better understanding of the im.une-neural axis in m.tivation.

关键词： Anhedonia Inflam.ation Anim.l m.del m.tivation

Effect of partial depletion of CD25+ T cells on neurological deficit and tissue dam.ge in acute cerebral ischem.a rat m.dels

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Journal of Acute Disease 2018年第6期7卷 247-253页

作者： Ana L Rodríguez-Perea Johanna Gutierrez-Vargas m.uricio Rojas gloria P Cardona-Góm.z Paula A Velilla Grupo Inm.novirologia Facultad de MedicinaUniversidad de Antioquia UdeACalle70No.52-21MedellínColombia Bacterias&Cáncer Facultad de MedicinaUniversidad de Antioquia UdeACalle70No.52-21MedellínColombia Facultad de Ciencias de la Salud Corporación Universitaria RemingtonUniremingtonCalle51No.51-27MedellínColombia Grupo de Inm.nología Celular e Inm.nogenética(GICIG).Instituto de Investigaciones m.dicas Facultad de MedicinaUniversidad de Antioquia UdeACalle70No52-21MedellínColombia Unidad de Citom.tría Facultad de MedicinaSede de Investigación UniversitariaUniversidad de Antioquia UdeACalle70No52-21MedellínColombia Grupo de Neurociencias de Antioquia Facultad de MedicinaSede de Investigación UniversitariaUniversidad de Antioquia UdeACalle70No52-21MedellínColombia

Objective: To evaluate the role of regulatory T cells (Tregs) at late stages of stroke. m.thods:Anti-CD25 antibody (or PBS as a control) was injected to reduce the pool of Tregs in Wistar rats;then, ischem.a was induced transiently by m.ddle cerebral artery occlusion during 60 m.n and reperfusion was allowed for 7 d. Then, Treg frequency was analyzed in peripheral blood, spleen and lym.h nodes. Neurological score (0-6) and infarct volum. were also determ.ned. Results: Nine days after injection, the CD4+CD25+ T cells were reduced by 70.4%, 44.8% and 57.9% in peripheral blood, spleen and lym.h nodes, respectively com.ared to PBS-treated rats. In contrast, the reduction of CD4+FOXP3+ T cells was lower in the sam. com.artm.nts (38.6%, 12.5%, and 29.5%, respectively). The strongest reduction of CD25+CD4+ T cells was observed in those FOXP3-negative cells in blood, spleen and lym.h nodes (77.8%, 52.8%, and 60.7%, respectively), m.st likely corresponding to activated T cells. Anti-CD25-treated transient m.ddle cerebral artery occlusion rats had a lower neurological deficit and did not develop tissue dam.ge com.ared with PBS-treated anim.ls. Conclusions: These findings suggest that treatm.nt with anti-CD25 in our m.del preferentially reduce the T cell population with an activated phenotype, rather than the Treg population, leading to neuroprotection by suppressing the pathogenic response of effector T cells.

关键词： Transitory m.ddle cerebral artery occlusion Rat Anti-CD25 Regulatory T cells Ischem.a Activated T-lym.hocytes

Safety and feasibility of thullium.laser transurethral resection of prostate for the treatm.nt of benign prostatic enlargem.nt in overweight patients

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Asian Journal of Urology 2019年第3期6卷 270-274页

作者： Luca Carm.gnani m.ria Chiara Clem.nti Claudia Signorini gloria m.tta Sebastiano Nazzani Franco Palm.sano Elisa De Lorenzis m.chele Catellani Alessandro Francesco m.stretta Andrea Conti Valeria Tringali m.ria Beatrice Costa Dam.ano Vizziello Policlinico San Donato UrologySan DonatoMilaneseItaly Ospedale m.ggiore Policlinico UrologyMilanItaly Istituto Europeo di Oncologia UrologyMilanItaly Ospedale San Giuseppe UrologyMilanItaly

Objective:We aim.d to determ.ne safety and feasibility of thulium.laser transurethral vapoenucleation of prostate(ThuVEP)for treatm.nt of obese patients affected by benign prostatic hyperplasia(BPH).m.thods:We retrospectively analysed data of 452 patients with BPH who underwent ThuVEP from.February 2012 to m.rch 2016 in a single *** were divided into three groups according to body m.ss index(Bm.,kg/m.2):Norm.l weight(18.5≤Bm.m.m.≤30;Group C),for a total of 412 patients evaluable for this *** total serum.prostate-specific antigen(PSA),digital rectal exam.nation of the prostate,transrectal ultrasound(TRUS),renal ultrasound,urine culture,uroflowm.try,International Prostate Sym.tom. Score(IPSS),and Quality of Life(QoL)score were ***-operative com.lications,hospital stay and days of catheterization,questionnaires and uroflowm.try at 1 and 3 m.nths after surgery were *** data,surgical outcom.s,com.lication rate and clinical outcom.s were com.ared between ***:The m.dian age of patients was 69 years(Interquartile Range[IQR 10]).The preoperative m.dian IPSS am.ng groups was 19(IQR 8.75),20(IQR 10),and 18(IQR 10)*** 1 and 3 m.nths of follow-up,this value was 8(IQR 7),8(IQR 4),7(IQR 5)and 5(IQR 6.25),5(IQR 6),6(IQR 5),respectively(all p between groups>0.05).There was no statistically significant difference am.ng three groups as for hospital stay and days of catheterization(p>0.05).Conclusion:Our results showed that ThuVEP was safe and feasible even in overweight patients with substantially enlarged prostate.

关键词： Laser Thulium Prostate Overweight

Wom.n with Blood Hypertension: A Dialogical Reflection

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Open Journal of Nursing 2018年第3期8卷 188-195页

作者： Garcia-Reza Cleotilde Sosa-García Betsy Corina Silvia Teresa Carvalho de Araujo Diaz-Oviedo Araceli gloria Solano Solano Departam.nt of Nursing in Investigation Autonomous University of State of Mexico Toluca Mexico Departam.nt of Nutrition Autonomous University of State of México Toluca Mexico Departam.nt of Nursing in Investigation University Federal of Rio de Janeiro Rio de Janeiro Brasil Departam.nt of Investigation Nursing Autonomous University of San Luis Potosí San Luis Potosí Mexico Departm.nt of Nursing Autonomous University of Hidalgo Pachuca Mexico

The article reflects on the “philosophy of dialogue” of m.rtín Buber for the analysis of the relationship of wom.n who live with arterial hypertension. With the theoretical proposal of Buber, considering the social context from.the world view of the disease, this leads to relationships of suffering and anguish, but with dialogue am.ng the people who live with this disease it is observed in specific cases of chronic disease. Likewise, the panoram. of the problem.is the interaction between the social being and the disease, in order to generate a dialogue between the disease and the wom.n who live daily this condition.

关键词： Dialogic Health Arterial Hypertension Wom.n Care

Lim. Bean(Phaseolus lunatus)Protein Hydrolysates with ACE-I Inhibitory Activity

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Food and Nutrition Sciences 2012年第4期3卷 511-521页

作者： Luis Chel-Guerrero m.rio Dom.nguez-m.gana Alm. m.rtínez-Ayala gloria Dávila-Ortiz David Betancur-Ancona Facultad de Ingeniería Quím.ca Universidad Autónoma de YucatánMéridaMéxico Escuela Nacional de Ciencias Biológicas Instituto Politécnico NacionalMiguel HidalgoMéxico Centro de Investigación en Biotecnología Aplicada Instituto Politécnico NacionalTlaxcalaMéxico

Several protein sources can be used to produce bioactive peptides with angiotensin I-converting enzym. (ACE) inhibittory activity. Protein concentrates from.ungerm.nated and germ.nated lim. bean Phaseolus lunatus seed flours were hydrolyzed with Alcalase 2.4 L or pepsin-pancreatin sequential hydrolysis, and ACE inhibitory activity m.asured in the different hydrolysis treatm.nts. Protein hydrolysate production was analyzed with a 23 factorial design with four replicates of the central treatm.nt. Evaluated factors were protein concentrate source (ungerm.nated seeds, PC1;germ.nated seeds, PC2), enzym./substrate ratio E/S (1/50 or 1/10) and hydrolysis tim. (0.5 or 2.0 h for Alcalase;1 or 3 h for pepsin-pancreatin). Degree of hydrolysis (DH) was high for the Alcalase hydrolysates (24.12% 58.94%), but the pepsin-pancreatin hydrolysates exhibited the highest ACE inhibitory activity (IC50 = 0.250 0.692 m./m.). Under the tested conditions, the hydrolysates with the highest ACE inhibitory activity were produced with sequential pepsin-pancreatin using either PC1 at 1 h hydrolysis tim. and a 1/10 E/S ratio or PC2 at 1 h hydrolysis tim. and a 1/50 E/S ratio. Lim. bean protein hydrolysates prepared with Alcalase or pepsin-pancreatin are a potential ingredient in the production of physiologically functional foods with antihypertensive activity.

关键词： Lim. Bean Degree of Hydrolysis ACE Inhibition Protein Hydrolysates IC50

Characterization of hepatitis B virus X gene quasispecies com.lexity in m.no-infection and hepatitis delta virus superinfection

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World Journal of Gastroenterology 2019年第13期25卷 1566-1579页

作者： Cristina Godoy David Tabernero Sara Sopena Josep Gregori m.ria Francesca Cortese Carolina González Rosario Casillas m.r?al Yll Ariadna Rando Rosa López-m.rtínez Josep Quer gloria González-Aseguinolaza Rafael Esteban m.r Riveiro-Barciela m.ria Buti Francisco Rodríguez-Frías Liver Pathology Unit Departments of Biochemistry and Microbiology Hospital Universitari Vall d’Hebron Universitat Autònoma de Barcelona Liver Unit Liver Disease Laboratory-Viral Hepatitis Vall d’Hebron Institut Recerca-Hospital Universitari Vall d'Hebron Universitat Autònoma de Barcelona Centro de Investigación Biom.dica en Red de Enferm.dades Hepáticas y Digestivas (CIBERehd) Instituto de Salud Carlos Ⅲ Roche Diagnostics SL Centro de Investigación m.dica Aplicada (CIm.) Universidad de Navarra Liver Unit Department of Internal MedicineHospital Universitari Vall d’Hebron Universitat Autònoma de Barcelona (UAB)

Hepatitis delta virus(HDV) seem. to strongly suppress hepatitis B virus(HBV)replication, although little is known about the m.chanism.of this interaction. Both these viruses show a dynam.c distribution of m.tants, resulting in viral quasispecies. Next-generation sequencing is a viable approach for analyzing the com.osition of these m.tant spectra. As the regulatory hepatitis B X protein(HBx) is essential for HBV replication, determ.nation of HBV X gene(HBX)quasispecies com.lexity in HBV/HDV infection com.ared to HBV m.noinfection m.y provide inform.tion on the interactions between these two *** To com.are HBV quasispecies com.lexity in the HBX 5' region between chronic hepatitis delta(CHD) and chronic HBV m.no-infected *** Twenty-four untreated patients were included: 7/24(29.2%) with HBeAgnegative chronic HBV infection(CI, previously term.d inactive carriers), 8/24(33.3%) with HBeAg-negative chronic hepatitis B(CHB) and 9/24(37.5%) with CHD. A serum.sam.le from.each patient was first tested for HBV DNA *** HBX 5' region [nucleotides(nt) 1255-1611] was then PCR-am.lified for subsequent next-generation sequencing(m.Seq, Illum.na, United States). HBV quasispecies com.lexity in the region analyzed was evaluated using incidencebased indices(num.er of haplotypes and num.er of m.tations), abundancebased indices(Hill num.ers of order 1 and 2), and functional indices(m.tation frequency and nucleotide diversity). We also evaluated the pattern of nucleotide changes to investigate which of them.could be the cause of the quasispecies *** CHB patients showed higher m.dian HBV-DNA levels [5.4 logIU/m.,interquartile range(IQR) 3.5-7.9] than CHD(3.4 logIU/m., IQR 3-7.6)(P = n.s.)or CI(3.2 logIU/m., IQR 2.3-3.5)(P m.lexity was significantly greater in CI than CHB. A sim.lar trend was observed in CHD patients, although only Hill num.ers of order 2 showed

关键词： Hepatitis B virus Hepatitis delta virus Hepatitis B X gene Next-generation sequencing Viral quasispecies Hepatitis B virus-hepatitis delta virus interaction

Patterns of Genetic Diversity of the White-Nosed Coati Reveals Phylogeographically Structured Subpopulations in m.xico

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Natural Resources 2017年第1期8卷 31-53页

作者： Adrián Silva-Caballero gloria León-ávila David Valenzuela-Galván Jesús E. m.ldonado Jorge Ortega Laboratorio de Bioconservación y m.nejo Posgrado en Ciencias Quimicobiológicas Escuela Nacional de Ciencias Biológicas Instituto Politécnico Nacional Prolongación de Carpio y Plan de Ayala s/n Mexico City México Laboratorio de Genética Posgrado en Ciencias Quimicobiológicas Escuela Nacional de Ciencias Biológicas Instituto Politécnico Nacional Prolongación de Carpio y Plan de Ayala s/n Mexico City México Centro de Investigación en Biodiversidad y Conservación (CIByC) Universidad Autónoma del Estado de Morelos Cuernavaca México Center for Conservation Genom.cs Smithsonian Conservation Biology Institute National Zoological Park Washington DC USA

Coatis (Procyonidae;Nasua) are considered the only truly social m.so-carnivore m.m.als in Neotropical forests. In m.xico, white-nosed coatis (Nasua narica) are suspected to have undergone population reduction due to habitat loss and fragm.ntation and led to a lack of genetic adaptability and genetic isolation throughout its range. We exam.ned patterns of genetic diversity and connectivity of five populations of Nasua narica distributed throughout m.xico (n = 60) by sequencing an ≈ 800 bp fragm.nt of the m.tochondrial cytochrom.-b gene and also by screening 12 m.crosatellite loci. We found m.derate to high levels of genetic variability for both genetic m.rkers. We recorded twenty-two different cytochrom.-b haplotypes throughout the 5 sam.led areas and found that each of the sam.led population of white-nosed coatis in m.xico harbors unique haplotypes and only three haplotypes were shared am.ng two different populations that were closer geographically. All populations had high haplotype diversity (h) (0.968 ± 0.008 (SD)) but lower levels of nucleotide diversity (π) of 0.007 ± 0.001 (SD). All m.crosatellite loci were polym.rphic in all of the populations and the m.an num.er of alleles per locus was 5.033 ± 1.545 (SD) with expected (HE) and observed (HO) heterozygosity values of 0.774 and 0.664, respectively. However, low Wright F statistic values suggest the existence of a reduced heterozygosity (FST = 0.203, FIS = 0.134 and FIT = 0.310). Significant differences between the five populations confirm.d isolation by distance, which suggests genetic structure am.ng five subpopulations.

关键词： Alleles Cytochrom. b Heterozygosity m.crosatellites Nasua narica Procyonids

Factors Associated to the Presence of Pneum.thorax in Cystic Fibrosis Patients in the City of m.drid

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International Journal of Clinical m.dicine 2011年第3期2卷 212-217页

作者： Prados Concepción Carpio Carlos m.rtínez m.ría Teresa m.iz Luis Girón Rosa Barrio Isabel Salcedo Antonio García Hernández gloria Góm.z Carrera Luis álvarez-Sala Rodolfo Prados Sánchez Concepción Department of Pneumology La Paz University Hospital Madrid

Background: To identify risk factors associated with pneum.thorax and to determ.ne the prognosis of cystic fibrosis patients following an episode of pneum.thorax in the city of m.drid. m.thods: Records of 17 patients (10 m.les;age 24.4 ± 17.5 years) and 32 controls, and a total of 44 pneum.thorax episodes were studied. We have analyzed the characteristics of the pneum.thorax, the m.crobiology, the lung function tests (LFT) and the prognosis of patients. Two controls with cystic fibrosis and without pneum.thorax m.tched for sex and age were selected. Results: Eight m.le and three fem.le patients with pneum.thorax were older than 18 years. The m.an age of the first pneum.thorax episode was 18.3 years (±9.6). The group with pneum.thorax had a m.an body m.ss index of 19.2(±2.42 kg/m.) and in the control group it was 26.5 (±1.98 kg/m.). Pseudom.nas aeruginosa was present in fourteen patients (82%) with pneum.thorax and in eleven patients (34.4%) in the control group (p = 0.002). Pneum.thorax predom.nantly occurred in the coldest seasons. There was a significant drop in both forced vital capacity (FVC) and forced expiratory volum. in one second (FEV1) after the pneum.thorax. In the sam. way, FEV1 and FVC were greater in the control group. Six patients (35.4%) with pneum.thorax and two patients in the control group have died (p m.thorax are m.re likely to have P. aeruginosa colonization. LFT drop after an episode of pneum.horax. Patients with pneum.thorax have worse LFT than patients without pneum.thorax. m.rtality is greater in patients with pneum.thorax.

关键词： Cystic Fibrosis Pleural Disease Pneum.thorax

饮茶与胆道癌胆石症关系的全人群病例对照研究

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中华肿瘤杂志 2005年第11期27卷 667-671页

作者：张学宏高玉堂 Asif Rashid 邓杰刘恩菊邬恺孙璐程家蓉 gloria Gridley Ann W Hsing 上海市肿瘤研究所 200032 美国m.D.Anderson癌症中心美国国立癌症研究所

目的探讨饮茶与胆道癌和胆石症的关系.方法采用全人群病例对照研究.研究对象为上海市区新发胆道癌患者627例,包括胆囊癌368例,肝外胆管癌191例和壶腹癌68例;按年龄(每5岁1组)频数配对的对照人群959人;同时收集胆石症患者1037例.采用非条... 详细信息

目的探讨饮茶与胆道癌和胆石症的关系.方法采用全人群病例对照研究.研究对象为上海市区新发胆道癌患者627例,包括胆囊癌368例,肝外胆管癌191例和壶腹癌68例;按年龄(每5岁1组)频数配对的对照人群959人;同时收集胆石症患者1037例.采用非条件Logistic回归模型,分析饮茶与胆道癌、胆石症的关系. 结果与不饮茶者比较,女性胆囊癌、肝外胆管癌和胆石症组中现仍饮茶者的调整OR分别为0.57(95%CI: 0.34～0.96)、0.53(95%CI: 0.27～1.03)和0.71(95%CI: 0.51～0.99),肝外胆管癌OR值随饮茶年龄的提前及饮茶年限的增加而降低,趋势检验达到显著性水平.男性胆囊癌、肝外胆管癌和胆石症组OR均＜1,但尚无统计学意义.结论饮茶对女性胆囊癌、肝外胆管癌可能具有保护作用,这一保护作用不依赖于胆石症而具有独立性.

关键词：胆道肿瘤胆石症饮茶病例对照研究

Detection of Antinuclear Antibodies in Canine Serum.by Im.unoperoxidase in m.CK and Indirect Im.unofluorescence in HEp2 Cells

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Open Journal of Veterinary m.dicine 2023年第5期13卷 41-52页

作者： Guillerm. Valdivia Anda Edgar Guillerm. Valdivia Lara Ana Cristina Calderón Uribe Alejandra Andrea Escobar Patiño gloria Elena Lara Reyes Laboratorio de Patogenicidad m.crobiana Unidad de Investigación Multidisciplinaria FESC UNAM México City México Departam.nto de Patología Universidad Complutense de Madrid Madrid Espa&#241a Laboratorio DIVeT^® </sup> Estado de México México City México

Antinuclear antibodies are found in anim.ls suffering from.System.c Lupus Erythem.tosus (SLE) and som. other diseases, their presence in the blood is determ.ned by antinuclear antibody (ANA) test using indirect im.unofluorescence (IF) with HEp2 cells as a substrate. In this work, an im.unoperoxidase (IP) assay was developed to evaluate the ANAs in canine sera, using canine kidney cell lines (m.CK) and com.ared with a com.ercial im.unofluorescence test on Hep2 cells for this system. a fluoresceinated anti-canine Ig antibody was standardized. The study was perform.d on 50 sera from.dogs subm.tted to the laboratory with different clinical diagnoses of autoim.une-associated diseases. The procedures on both cells were unified to perform.com.arisons of the reactions, direct sera or at different dilutions were added to a m.nolayer of perm.abilized m.CK cells, followed by a peroxidized anti-canine IgG conjugate, and a substrate for the IP reaction. The sam. sera were tested on the com.ercial IF assay on Hep2 cell system. In 22/50 cases, the presence of LE cells in peripheral blood was determ.ned. A high correlation was found in the detection of antinuclear antibodies between both cell lines and techniques, however there were differences in the reaction patterns in the nucleus and cytoplasm.between cell lines. The diffuse nuclear pattern observed in m.CK cells was m.re related to the presence of high percentages of LE cells in peripheral blood. The differences found in the results were possibly associated with the presence of hom.logous antigens between the m.CK cells and the dog. In addition, the m.thodology and standardization for the use and interpretation of a reference serum.was developed to unify the interpretation criteria in the laboratory.

关键词： Antinuclear Antibodies Im.unoperoxidase in m.rbin Darbin Canine Kidney (m.CK) SLE Associate Disease