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Synsepalum dulcificum extracts exhibit cytotoxic activity on human colorectal cancer cells and upregulate c-fos and c-jun early apoptotic gene expression

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Asian Pacific Journal of Tropical Biomedicine 2018年第3期8卷 173-178页

作者： Jichang Seong glenn g.oyong Esperanza C.Cabrera Biology Department De La Salle University Molecular Science Unit Laboratory Center for Natural Science and Environmental Research De La Salle University

Objective: To explore cytotoxicity of Synsepalum dulcificum(S. dulcificum) Daniell(Sapotaceae) on human colon cancer(HCT-116 and HT-29), human monocytic leukemia(THP-1) and normal(HDFn) cell lines, and its effect on the expression of early apoptotic genes, c-fos and c-jun. Methods: Leaf, stem and berry of S. dulcificum were separately extracted by using 2 solvents, 10% ethanol(EtOH) and 80% methanol(MeOH). PrestoB lue~? cell viability assay and q RT-PCR assay were conducted to examine the above objectives respectively. Results: Stem MeOH, stem EtOH, and berry EtOH extracts of S. dulcificum were cytotoxic to HCT-116 and HT-29 human colon cancer cells. For HCT-116, IC_(50) values of these 3 extracts were not significantly different(P>0.05) from that of the positive control bleomycin(IC_(50) of 33.57 μg/mL), while for HT-29, IC_(50) values of these 3 extracts were significantly lower(Pg/mL). None of the extracts were cytotoxic to the THP-1 monocytic leukemia cells and HDFn normal human dermal fibroblasts. For both HCT-116 and HT-29, these extracts significantly up-regulated(Pgative control. Conclusions: The results of this study suggest that cytotoxicity of stem MeOH, stem EtOH, and berry EtOH extracts of S. dulcificum on HCT-116 and HT-29 colon cancer cells is due to the induced apoptosis which is caused by the up-regulation of the expression of early apoptotic genes, c-fos and c-jun.

关键词： Synsepalum dulcificum Colon cancer Monocytic leukemia Apoptosis c-fos c-jun

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Maternal reproductive health: Expression patterns of antioxidant enzyme selenoproteins of post-implantation embryos conceived by ethanol-treated murine mothers supplemented with α-tocopherol

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Asian pacific Journal of Reproduction 2017年第1期6卷 21-25页

作者： gliceria B Ramos Noel F Alfonso glenn g oyong Aleli Jillian L Sia Biology Department De La Salle UniversityTaft AvenueManilaPhilippines1004 University Science Foundation-University Research Coordination Office (USF-URCO)De La Salle UniversityManilaPhilippines Molecular Biology Unit CENSERDe La Salle UniversityTaft AvenueManilaPhilippines Department of Biology College of Arts and SciencesUniversity of the Philippines-Manila

Objective:To investigate if the protective effect ofα-tocopherol against the impact of ethanol on brain morphogenesis involved the activity of the selenoproteins phospholipid hydroperoxide glutathione peroxidase (PHgPx;gPx4) and selenoprotein P (SelPP) that have roles against oxidative ***:Forty female mice were randomly assigned into natural control (CON), positive control (ETOH), low-, medium-, and high-α-tocopherol-supplemented-ethanol groups (LTOC, MTOC, HTOC, respectively). CON received drinking water without ethanol while ETOH, LTOC, MTOC and HTOC groups received 20% ethanol in drinking water. The supplemented groups were given respective dosages ofα-tocopherol, 0.410, 0.819, and 1.640 mg/g body weight, at day 14 before mating onwards to the day 9 of gestation. At 10.5 ED of gestation (1100 h), the pregnant females were sacrificed by cervical dislocation and the embryos were harvested. Total RNA were extracted, cDNA synthesis and qRT- PCR analyses were carried ***: The level of expression of PHgPx in the positive control was significantly lower than that of the natural control. Among the threeα-tocopherol-supplemented groups, only the medium dose- group was significantly higher than the positive control. The level of expression of SelPP in the positive control was significantly lower than those of the natural control, the low- and medium- dose -tocopherol supplemented groups. In the high dose-α-tocopherol supplemented group, the level of expression was not significantly different from the positive control but significantly lower than the natural ***: The activity of the selenoproteins PHgPx and SelPP are involved in the internetwork of antioxidative enzymes with vitamin E when given up to a medium dose only and is one of the possible pathways of shielding embryonic development against the impact of ethanol on brain morphogenesis. This study strengthens the impact of dietaryα-tocopherol and Selenium supplement during the criti

关键词： α-tocopherol Dysmorphogenesis Ethanol PHgPx Postimplantation embryos Selenoproteins SelPP

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Antioxidant,cytotoxic,and anti-venom activity of Alstonia parvifolia ***

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Asian Pacific Journal of Tropical Biomedicine 2021年第10期11卷 460-468页

作者： Maria Carmen S.Tan Mary Stephanie S.Carranza Virgilio C.Linis Raymond S.Malabed Yves Ira A.Reyes Francisco C.Franco,Jr. glenn g.oyong Chemistry Department De La Salle University2401 Taft AvenueManila 1004Philippines Biology Department De La Salle University2401 Taft AvenueManila 1004Philippines Department of Chemistry Graduate School of ScienceOsaka UniversityOsaka 563-0034Japan Molecular Science Unit Laboratory Center for Natural Sciences and Environmental ResearchDe la Salle University2401 Taft AvenueManila 1004Philippines

Objective:To evaluate antioxidant,cytotoxic,and anti-venom capacity of crude bark extracts of Alstonia parvifolia ***:gas chromatography-mass spectrometry(gC-MS)and energy dispersive X-ray analyses were accomplished to characterize the chemical constituents of Alstonia *** characterization was evaluated using an inhibitory phospholipase A_(2)(PLA_(2))assay,DPPH,and cytotoxicity *** the constituents listed in the gC-MS analyses,molecular docking was conducted to inspect the binding energies between the chosen compounds and selected PLA_(2) ***:gC-MS analyses showed that the Alstonia parvifolia crude extract consisted predominantly of acetylmarinobufogenin(14.89%),γ-sitosterol(10.44%),3-O-methyl-D-glucose(5.88%),3,5-dimethoxy-4-hydroxyphenylacetic acid(5.30%),(2α,5α)-17-methoxyaspidofractinin-3-one(AFM)(4.08%),and 2,3,5,6,7,8,9-heptahydro-1-phenyl-5-(p-chlorophenylimino)-1H-benzo[e][1,4]thiazepine(HPT)(1.37%).The principal elemental components of Alstonia parvifolia were Ca(4.012%)and K(1.496%),as exhibited by energy dispersive X-ray *** parvifolia showed significant free radical scavenging ability(IC_(50):0.287 mg/mL)and was non-cytotoxic to normal HDFn cells(IC_(50)>100μg/mL).Moreover,Alstonia parvifolia was favorably cytotoxic to MCF-7(IC_(50):4.42µg/mL),followed by H69PR,HT-29,and THP-1,with IC_(50) values of 4.94,5.07,and 6.27µg/mL,*** parvifolia also displayed notable inhibition against PLA_(2) activity of Naja philippinensis Taylor venom with IC_(50) of(15.2±1.8)μg/*** and cluster analyses projected negative binding energies from AFM(−6.36 to−9.68 kcal/mol),HPT(−7.38 to−9.77 kcal/mol),and acetylmarinobufogenin(−7.22 to−9.59 kcal/mol).These calculations were for the particular interactions of Alstonia parvifolia constituents to PLA_(2) homologues where the utmost affinity was detected in HPT owing to the dipole interactions with amino acid ***:The bark e

关键词： Alstonia parvifolia Merr. Naja philippinensis Taylor gas chromatography-electron ionization-mass spectrometry Secretory phospholipase A_(2) Cytotoxicity assay Anti-venom

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